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Am J Physiol Regul Integr Comp Physiol ; 306(2): R108-17, 2014 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-24305064

RESUMEN

Despite the fact that urogenito-sexual complications significantly impact the quality of life of diabetic patients, a robust in vivo experimental model is lacking. Bladder and erectile function in the Type 2 diabetic Goto-Kakizaki (GK) rat and responses to standard-of-care treatments for each disorder have been assessed. GK rats (n = 25, 18-wk-old, GK/Par colony) and age-matched Wistar rats (n = 23), characterized for their metabolic parameters, were used. Bladder function was assessed by cystometry in conscious rats treated by intravenous solifenacin (1 mg/kg). Subsequently, erectile function was assessed under anesthesia following electrical stimulation of the cavernous nerve in presence of intravenous sildenafil (0.3 mg/kg). GK rats displayed detrusor overactivity with a significant increase in frequency/amplitude of nonvoiding contractions during the filling phase, together with an increase in bladder capacity, intercontraction interval, voided volume, and maximal pressure of voiding contraction. Solifenacin significantly decreased parameters characterizing voiding contractions without modifying voiding efficiency. Erectile function in GK rats was markedly impaired and remained so after sildenafil treatment despite a significant improvement. GK rats display both bladder and erectile dysfunctions and respond at least partially to standard-of-care treatments for each disorder, thus representing a suitable model to investigate the pathophysiology and assess the efficacy of new therapeutic agents for Type 2 diabetes-associated bladder and erectile complications.


Asunto(s)
Complicaciones de la Diabetes/fisiopatología , Diabetes Mellitus Tipo 2/complicaciones , Disfunción Eréctil/etiología , Enfermedades de la Vejiga Urinaria/etiología , Animales , Diabetes Mellitus Tipo 2/fisiopatología , Masculino , Erección Peniana/efectos de los fármacos , Piperazinas/farmacología , Purinas/farmacología , Quinuclidinas/farmacología , Ratas , Ratas Wistar , Citrato de Sildenafil , Succinato de Solifenacina , Sulfonas/farmacología , Tetrahidroisoquinolinas/farmacología , Vejiga Urinaria/fisiología , Enfermedades de la Vejiga Urinaria/tratamiento farmacológico , Enfermedades de la Vejiga Urinaria/fisiopatología , Micción/efectos de los fármacos , Micción/fisiología , Agentes Urológicos/farmacología , Vasodilatadores/farmacología
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