RESUMEN
To determine the role of the reactive stroma in cancer progression, we investigated decorin (DCN) and transforming growth factor-beta (TGF-beta expression, and matrix metalloproteinase-2 (MMP-2) activity in the tumorous esophagus. We found statistically insignificantly decreased levels of DCN expression in the pathological tissues. No obvious alterations in TGF-beta expression were noticed. The highly significant increase in MMP-2 activity in cancers did not result in elevated levels of TGF-beta dimers. Therefore, the system of TGF-beta liberation from its complex with DCN by activated MMP-2 does not seem to contribute to esophageal cancerogenesis, although this hypothesis should be reevaluated with a larger study group.
Asunto(s)
Carcinoma de Células Escamosas/química , Neoplasias Esofágicas/química , Proteínas de la Matriz Extracelular/análisis , Metaloproteinasa 2 de la Matriz/análisis , Proteoglicanos/análisis , Factor de Crecimiento Transformador beta/análisis , Adulto , Anciano , Carcinoma de Células Escamosas/enzimología , Carcinoma de Células Escamosas/genética , Decorina , Activación Enzimática , Neoplasias Esofágicas/enzimología , Neoplasias Esofágicas/genética , Proteínas de la Matriz Extracelular/genética , Femenino , Regulación Enzimológica de la Expresión Génica , Regulación Neoplásica de la Expresión Génica , Humanos , Inmunohistoquímica , Masculino , Metaloproteinasa 2 de la Matriz/genética , Persona de Mediana Edad , Isoformas de Proteínas , Proteoglicanos/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células del Estroma/química , Factor de Crecimiento Transformador beta/genética , Factor de Crecimiento Transformador beta1/análisis , Factor de Crecimiento Transformador beta2/análisis , Factor de Crecimiento Transformador beta3/análisisRESUMEN
The aim of this paper was to examine participation of the epidermal growth factor receptor (EGF-R) signal pathway in the pathogenesis of Dupuytren's disease. The study showed changes in the ratio of membrane EGF-R to its intracellular level during the different clinical stages of Dupuytren's contracture progression. Our observations of a high ratio of surface to intracellular EGF-R in the palmar aponeurosis of patients with second degree of Dupuytren's disease (Iselin's classification), which was significantly higher than this ratio in control palmar fascia (P=0.022), would suggest that EGF-R has a role in the involutional phase of the disease.