Enhanced extracellular vesicles mediated uttroside B (Utt-B) delivery to Hepatocellular carcinoma cell: Pharmacokinetics based on PBPK modelling.

Our prior investigation has confirmed that the anti-hepatocellular carcinoma activity of the plant saponin, specifically Uttroside B (Utt-B), derived from the leaves of Solanum nigrum Linn. This study concentrated on formulating a novel biocompatible nanocarrier utilizing Extracellular vesicles (EVs) to enhance the delivery of plant saponin into cells. The physicochemical attributes of Extracellular Vesicles/UttrosideB (EVs/Utt-B) were comprehensively characterized through techniques such as Transmission Electron Microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Despite the promising therapeutic potential of this uttroside B, mechanistic know-how about its entry into cells is still in its infancy. Our research sheds light on the extracellular vesicle-mediated mechanism facilitating the entry of the saponin into cells, a phenomenon confirmed through the use of by confocal microscopy. We further analysed drug-releasing kinetics and simulated the Pharmacokinetics by PBPK modelling. The simulated pharmacokinetics revealed the bioavailability of Uttroside-B in oral administration against intravenous administration.

Nano-remediation of toxic heavy metal contamination: Hexavalent chromium [Cr(VI)].

The inexorable industrialization and modern agricultural practices to meet the needs of the increasing population have polluted the environment with toxic heavy metals such as Cr(VI), Cu2+, Cd2+, Pb2+, and Zn2+. Among the hazardous heavy metal(loid)s contamination in agricultural soil, water, and air, hexavalent chromium [Cr(VI)] is the most virulent carcinogen. The metallurgic industries, tanneries, paint manufacturing, petroleum refineries are among various such human activities that discharge Cr(VI) into the environment. Various methods have been employed to reduce the concentration of Cr(VI) contamination with nano and bioremediation being the recent advancement to achieve recovery at low cost and higher efficiency. Bioremediation is the process of using biological sources such as plant extracts, microorganisms, and algae to reduce the heavy metals while the nano-remediation uses nanoparticles to adsorb heavy metals. In this review, we discuss the various activities that liberate Cr(VI). We then discuss the various conventional, nano-remediation, and bioremediation methods to keep Cr(VI) concentration in check and further discuss their efficiencies. We also discuss the mechanism of nano-remediation techniques for better insight into the process.