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1.
Public Health ; 225: 311-319, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-37972494

RESUMEN

OBJECTIVES: In 2020, Brazil experienced two concurrent public health challenges related to respiratory disease: wildfires and increased mortality due to the coronavirus (COVID-19) pandemic. Smoke from these wildfires contributed to a variety of air pollutants, including fine particulate matter (PM2.5). The present study aims to investigate the effects of environmental and socio-economic factors on COVID-19 hospitalisation in the Pantanal. STUDY DESIGN: Ecological retrospective study. METHODS: We applied a multilevel negative binomial model to relate monthly hospitalisation data with environmental variables. RESULTS: We showed that monthly PM2.5 concentration levels had the greatest influence on the increase in hospitalisations by COVID-19 in the elderly (23 % increase). The Gini index, a coefficient that reflects income inequalities, also had a positive association with COVID-19 hospitalisations (18 % increase). Higher temperatures and humidity were protective factors, showing a 15 % and 14 % decrease in hospitalisations, respectively. The results of the present study suggest that high PM2.5 exposure contributed to the increase in COVID-19 hospitalisations, as did the social inequalities of each municipality. CONCLUSIONS: The present study highlights the importance of gathering evidence supported by multiple information sources to guide decision-making and identify populations needing better public health systems.


Asunto(s)
COVID-19 , Incendios Forestales , Humanos , Anciano , Humo/efectos adversos , Humedales , Estudios Retrospectivos , Brasil/epidemiología , COVID-19/epidemiología , Material Particulado/análisis
2.
Measurement (Lond) ; 181: 109589, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-36540695

RESUMEN

The importance of measurement quality cannot be over emphasized in medical applications, as one is dealing with life issues and the wellbeing of society, from oncology to new-borns, and more recently to patients of the COVID-19 pandemic. In all these dire situations, the accuracy of fluid delivered according to a prescribed dose can be critical. Microflow applications are growing in importance for a wide variety of scientific fields, namely drug development and administration, Organ-on-a-Chip, or bioanalysis, but accurate and reliable measurements are a tough challenge in micro-to-femto flow operating ranges, from 2.78 × 10-4 mL/s down to 2.78 × 10-7 mL/s (1000 µL/h down to 1 µL/h). Several sources of error have been established such as the mass measurement, the fluid evaporation dependent on the gravimetric methodology implemented, the tube adsorption and the repeatability, believed to be closely related to the operating mode of the stepper motor and drive screw pitch of a syringe pump. In addition, the difficulty in dealing with microflow applications extends to the evaluation of measurement uncertainty which will qualify the quality of measurement. This is due to the conditions entailed when measuring very small values, close to zero, of a quantity such as the flow rate which is inherently positive. Alternative methods able to handle these features were developed and implemented, and their suitability will be discussed.

3.
Glob Environ Change ; 48: 97-107, 2018 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-29606806

RESUMEN

Extreme climatic events are likely to become more frequent owing to global warming. This may put additional stress on critical infrastructures with typically long life spans. However, little is known about the risks of multiple climate extremes on critical infrastructures at regional to continental scales. Here we show how single- and multi-hazard damage to energy, transport, industrial, and social critical infrastructures in Europe are likely to develop until the year 2100 under the influence of climate change. We combine a set of high-resolution climate hazard projections, a detailed representation of physical assets in various sectors and their sensitivity to the hazards, and more than 1100 records of losses from climate extremes in a prognostic modelling framework. We find that damages could triple by the 2020s, multiply six-fold by mid-century, and amount to more than 10 times present damage of €3.4 billion per year by the end of the century due only to climate change. Damage from heatwaves, droughts in southern Europe, and coastal floods shows the most dramatic rise, but the risks of inland flooding, windstorms, and forest fires will also increase in Europe, with varying degrees of change across regions. Economic losses are highest for the industry, transport, and energy sectors. Future losses will not be incurred equally across Europe. Southern and south-eastern European countries will be most affected and, as a result, will probably require higher costs of adaptation. The findings of this study could aid in prioritizing regional investments to address the unequal burden of impacts and differences in adaptation capacities across Europe.

4.
Glob Chang Biol ; 23(2): 767-781, 2017 02.
Artículo en Inglés | MEDLINE | ID: mdl-27474896

RESUMEN

Understanding uncertainties in land cover projections is critical to investigating land-based climate mitigation policies, assessing the potential of climate adaptation strategies and quantifying the impacts of land cover change on the climate system. Here, we identify and quantify uncertainties in global and European land cover projections over a diverse range of model types and scenarios, extending the analysis beyond the agro-economic models included in previous comparisons. The results from 75 simulations over 18 models are analysed and show a large range in land cover area projections, with the highest variability occurring in future cropland areas. We demonstrate systematic differences in land cover areas associated with the characteristics of the modelling approach, which is at least as great as the differences attributed to the scenario variations. The results lead us to conclude that a higher degree of uncertainty exists in land use projections than currently included in climate or earth system projections. To account for land use uncertainty, it is recommended to use a diverse set of models and approaches when assessing the potential impacts of land cover change on future climate. Additionally, further work is needed to better understand the assumptions driving land use model results and reveal the causes of uncertainty in more depth, to help reduce model uncertainty and improve the projections of land cover.


Asunto(s)
Cambio Climático , Incertidumbre , Clima , Planeta Tierra , Predicción , Plantas
5.
J Dairy Sci ; 99(3): 2010-2015, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26778314

RESUMEN

The aim of this study was to compare pregnancy per artificial insemination (P/AI) from service sires used on artificial insemination after estrus detection (EAI) or timed artificial insemination (TAI) breedings. Confirmed artificial insemination outcome records from 3 national data centers were merged and used as a data source. Criteria edits were herd's overall P/AI within 20 and 60%, a minimum of 30 breedings reported per herd-year, service sires that were used in at least 10 different herds with no more than 40% of the breedings performed in a single herd, breeding records from lactating Holstein cows receiving their first to fifth postpartum breedings occurring within 45 to 375 d in milk, and cows with 1 to 5 lactations producing a minimum of 6,804 kg. Initially 1,142,859 breeding records were available for analysis. After editing, a subset of the data (n=857,539) was used to classify breeding codes into either EAI or TAI based on weekly insemination profile in each individual herd. The procedure HPMIXED of SAS was used and took into account effects of state, farm, cow identification, breeding month, year, parity, days in milk at breeding, and service sire. This model was used independently for the 2 types osires f breeding codes (EAI vs. TAI), and service sire P/AI rankings within each breeding code were performed for sires with >700 breedings (94 sires) and for with >1,000 breedings (n=56 sires) following both EAI and TAI. Correlation for service sire fertility rankings following EAI and TAI was performed with the PROC CORR of SAS. Service sire P/AI rankings produced with EAI and TAI were 0.81 (for sires with >700 breedings) and 0.84 (for sires with >1,000 breedings). In addition, important changes occurred in service sire P/AI ranking to EAI and TAI for sires with less than 10,000 recorded artificial inseminations. In conclusion, the type of breeding strategy (EAI or TAI) was associated with some changes in service sire P/AI ranking, but ranking changes declined as number of breedings per service sire increased. Future randomized studies need to explore whether changes in P/AI ranking to EAI versus TAI are due to specific semen characteristics.


Asunto(s)
Bovinos/fisiología , Fertilidad , Inseminación Artificial/veterinaria , Animales , Cruzamiento , Estro , Detección del Estro , Femenino , Inseminación Artificial/métodos , Lactancia , Masculino , Leche , Embarazo , Semen
6.
Genet Mol Res ; 15(2)2016 May 25.
Artículo en Inglés | MEDLINE | ID: mdl-27323091

RESUMEN

This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated. COX III mRNA expression in breast muscle was higher in the group fed with 12% glycerol (0.863 AU); no differences were observed in the expression of this gene between the muscle of animals fed diets without glycerol (0.357 AU) and 8% glycerol (0.415 AU). Quails that showed greater COX III mRNA expression also showed the lowest feed efficiency. These results show that there is a difference in COX III mRNA expression in breast muscle of 28-day-old quail fed diets different concentrations of glycerol.


Asunto(s)
Complejo IV de Transporte de Electrones/biosíntesis , Glicerol/administración & dosificación , Músculo Esquelético/efectos de los fármacos , Codorniz/genética , Alimentación Animal , Animales , Dieta , Complejo IV de Transporte de Electrones/genética , Regulación de la Expresión Génica/efectos de los fármacos , Músculo Esquelético/crecimiento & desarrollo , Músculo Esquelético/metabolismo , Codorniz/metabolismo , ARN Mensajero/biosíntesis , ARN Mensajero/genética
7.
Asian-Australas J Anim Sci ; 29(12): 1710-1718, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-27004813

RESUMEN

The objective of this study was to evaluate the effects of supplements with different crude protein (CP) contents on grazing cattle intake, digestibility, ruminal fermentation pattern, and nitrogen (N) metabolism characteristics during the rainy season. Five ruminal and abomasal cannulated Holstein×Zebu steers (296 kg body weight, BW) were used in a 5×5 Latin square design. The animals grazed five signal grass paddocks (0.34 ha). The five treatments evaluated were: Control (no supplement) and 1.0 g of supplement/kg BW with 0, 330, 660, and 1,000 g of CP/kg as-fed. The supplement was composed of starch, soybean meal, urea, and ammonium sulphate. There was a positive linear effect (p≤0.033) of the CP content in the supplements on the organic matter (OM), CP, and digested OM intakes. The provision of supplements did not increase (p≥0.158), on average, total and ruminal digestibilities of OM and CP. However, the increase in CP content in the supplements caused a positive linear effect (p≤0.018) on ruminal digestibilities of OM and CP. Additionally, a quadratic effect of the CP contents of the supplements were observed (p = 0.041) for the ruminal digestibility of neutral detergent fiber corrected for ash and protein, with the highest estimate obtained with the CP content of 670 g/kg. The supply of supplements increased (p<0.001) the ruminal ammonia N concentration, which also changed linearly and positively (p<0.001) according to increase in CP content in the supplements. The apparent N balance and relative N balance (g/g N intake) were not, on average, changed (p≥0.164) by the supplements supply. However, both showed a tendency of a linear increase (p≤0.099) with increasing supplement CP content. The supplements increased (p = 0.007) microbial N production in the rumen, which also changed linearly and positively (p = 0.016) with increasing supplement CP content. In conclusion, protein supplementation in grazing cattle during the rainy season, while stimulating voluntary forage intake, results in higher efficiency of N utilization when compared to energy supplementation. This is a possible response to increased microbial protein synthesis in the rumen and improved N status in the animal body.

8.
Br Poult Sci ; 56(1): 6-14, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25490970

RESUMEN

1. The objective of this study was to estimate growth parameters of carcass components (wing, thighs and drumsticks, back and breast) and organs (heart, liver, gizzard and gut) in males and females of one meat-type quail strain (Coturnix coturnix coturnix) and two laying strains (Coturnix coturnix japonica) designated either yellow or red. 2. A total of 1350 quail from 1 to 42 d old were distributed in a completely randomised design, with 5 replicates of each strain. The carcass component weights and body organs were analysed weekly and evaluated using the Gompertz function; growth rates were evaluated through derivative equations. 3. The meat-type strain presented the highest growth rates in carcass components and organs. Across strains, females showed the highest weight of internal organs at maturity compared to males. 4. Females had greater growth potential in breast, wings and back than males for both yellow and red laying quail.


Asunto(s)
Composición Corporal , Coturnix/crecimiento & desarrollo , Animales , Coturnix/genética , Femenino , Masculino , Carne , Modelos Biológicos , Tamaño de los Órganos , Factores Sexuales
9.
Int Endod J ; 47(9): 827-34, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24298979

RESUMEN

AIM: To evaluate the effects of topical Resolvin E1 (RvE1) application on infected dental pulps. METHODOLOGY: Forty-two male Wistar rats (n = 6 per three groups/and two time periods) were used. To induce inflammation, pulps in mandibular right first molars were accessed and then left exposed to the oral environment for 24 h. After this period, topical medication with a corticosteroid/antibiotic blend, or RvE1, or its vehicle (Ethanol 0.1%) was directly applied onto the pulp tissue and teeth were restored with silver amalgam. The effects of the protocols were evaluated histologically and compared with control pulps not exposed to the oral environment. The inflammatory changes after 24 and 72 h were assessed through a scoring method and analysed using the Kruskal-Wallis test followed by Dunn's. Differences were considered significant if P < 0.05 (CI = 95%). RESULTS: Ethanol and corticosteroid/antibiotic treatment were not effective in arresting severe inflammatory alterations of exposed pulps at 24 and 72 h (P < 0.05, CI = 95%). At both time periods, RvE1 treatment led to a reduction of tissue cellularity and extent of inflammation, whose changes were not different from control pulps (P > 0.05, CI = 95%). CONCLUSIONS: A protective role for RvE1 in pulp inflammation was observed even in the presence of contamination, suggesting that it may be a candidate for a novel therapeutic strategy for conservative dental pulp treatment.


Asunto(s)
Pulpa Dental/efectos de los fármacos , Ácido Eicosapentaenoico/análogos & derivados , Corticoesteroides/administración & dosificación , Corticoesteroides/farmacología , Animales , Antibacterianos/administración & dosificación , Antibacterianos/farmacología , Pulpa Dental/patología , Ácido Eicosapentaenoico/farmacología , Masculino , Ratas , Ratas Wistar
10.
Genet Mol Res ; 13(3): 4940-8, 2014 Jul 04.
Artículo en Inglés | MEDLINE | ID: mdl-25062481

RESUMEN

We evaluated the adenine nucleotide translocator (ANT) and cytochrome oxidase subunit III (COX III) mRNA expressions in the muscle and liver of Japanese quails presenting high and low feed efficiency (FE), and subjected them to three different environmental temperatures: comfort, heat stress (38°C), and cold stress (10°C). ANT mRNA expression was lower in the liver of heat-stressed animals. In the muscle, higher ANT and COX III mRNA expressions were observed in high-FE and cold-stressed animals. In the liver, much higher expression of COX III mRNA was observed in cold-stressed animals. These results suggest a possible correlation between the genes involved in energy production by the mitochondria and FE phenotypes, and that environmental temperature can affect the ANT and COX III mRNA expressions. Japanese quails presenting different FE levels respond differently to environmental stimuli.


Asunto(s)
Coturnix/genética , Complejo IV de Transporte de Electrones/genética , Hígado/enzimología , Mitocondrias/genética , Translocasas Mitocondriales de ADP y ATP/genética , Músculo Esquelético/enzimología , Animales , Frío , Coturnix/metabolismo , Ingestión de Alimentos/genética , Complejo IV de Transporte de Electrones/metabolismo , Metabolismo Energético/genética , Regulación de la Expresión Génica , Calor , Mitocondrias/metabolismo , Translocasas Mitocondriales de ADP y ATP/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Estrés Fisiológico
11.
Reprod Domest Anim ; 49(6): 1015-20, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25291989

RESUMEN

This study was performed to evaluate plasma concentrations of anti-Mullerian hormone (AMH) and the ovarian antral follicle population (AFP) in different genetic groups. Cyclic heifers (13 Bubalus bubalis [Murrah]; 15 Bos taurus [Holstein] and 10 Bos indicus [Gyr]) were maintained under the same management and were synchronized with two doses of 150 µg IM d-cloprostenol administered 14 days apart. After the second d-cloprostenol treatment, heifers had their ovaries scanned daily by ultrasound to define the day of ovulation. On the same day, the AFP was determined and a plasma sample was collected to measure AMH. Murrah heifers had less AFP (25.6 ± 2.1 follicles; p = 0.01) and plasma AMH concentration (0.18 ± 0.03 ng/ml; p < 0.001) than Gyr (60.0 ± 12.2 follicles and 0.60 ± 0.12 ng/ml of AMH); however, data were similar when compared to Holstein (35.9 ± 6.8 follicles and 0.24 ± 0.06 ng/ml of AMH) heifers. Regardless of genetic background, there was a positive relationship between the AFP and plasmatic AMH concentration (Murrah [r = 0.62; p < 0.01], Holstein [r = 0.66; p < 0.001] and Gyr [r = 0.88; p < 0.001]). Also, when heifers were classified according to high- or low-AMH concentration based on the average within each genetic group, high-AMH heifers had greater (p < 0.0001) AFP than low-AMH heifers. In conclusion, both Murrah and Holstein heifers presented lower plasma AMH concentration and AFP when compared to Gyr.


Asunto(s)
Hormona Antimülleriana/metabolismo , Búfalos/metabolismo , Bovinos/metabolismo , Cloprostenol/farmacología , Folículo Ovárico/efectos de los fármacos , Crianza de Animales Domésticos , Animales , Búfalos/sangre , Bovinos/sangre , Sincronización del Estro/métodos , Femenino , Folículo Ovárico/fisiología , Especificidad de la Especie
12.
Reprod Domest Anim ; 49(3): 448-52, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24689827

RESUMEN

In Bos taurus cattle, antimullerian hormone (AMH) has been demonstrated to have a high degree of correlation with ovarian antral follicle count and the number of healthy follicles and oocytes. To document the correlation between the plasma concentration of AMH and follicular number in Bos indicus and Bos taurus heifers, Nelore (Bos indicus, n = 16) and Holstein heifers (Bos taurus, n = 16) had their ovarian follicular waves synchronized. After synchronization, ovarian antral follicular population (AFP) was evaluated three times at 60-day (d) intervals (T-120 d, 120 days before plasma AMH determination; T-60 d, 60 days before; and T0, at the time of plasma AMH determination). The plasma AMH concentration was positively correlated with the number of ovarian follicles on the day of the follicular wave emergence in Bos indicus (Nelore) and Bos taurus (Holstein) heifers at each evaluation time (p < 0.05). The AFP was higher in Bos indicus (Nelore) than in Bos taurus (Holstein) heifers (p < 0.05). Similarly, the AMH concentration was higher in Bos indicus (Nelore) than in Bos taurus (Holstein) heifers (p < 0.0001). When heifers were classified as to present high or low AFP according to the mean of the AFP within each genetic group, high-AFP heifers presented a greater (p < 0.0001) AMH concentration than low-AFP heifers, regardless of the genetic group. In conclusion, the AFP is positively correlated with plasma AMH concentration in both Bos indicus (Nelore) and Bos taurus (Holstein) heifers. Furthermore, Bos indicus (Nelore) heifers presented both greater plasma AMH concentrations and AFP than Bos taurus (Holstein) heifers.


Asunto(s)
Hormona Antimülleriana/sangre , Bovinos/sangre , Folículo Ovárico/anatomía & histología , Animales , Femenino , Folículo Ovárico/diagnóstico por imagen , Especificidad de la Especie , Ultrasonografía
13.
Br Poult Sci ; 55(4): 466-73, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24848692

RESUMEN

1. A study was conducted to test the hypothesis that feed efficiency (FE) correlated with the expression of genes from the somatotropic axis and mitochondrial genes involved in energy production, and that the environment to which the birds are exposed influenced the expression of such genes. 2. Quails were divided into High-FE and low-FE groups and maintained in a comfortable or heat stress (HS) (38°C for 24 h) environment to evaluate changes in insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), adenine nucleotide translocator (ANT), uncoupling protein (UCP) and cytochrome oxidase subunit III (COX III) messenger ribonucleic acid (mRNA) expression in liver and muscle tissues. 3. High-FE quails (0.28 g/g) presented a higher final body weight, greater weight gain and a better feed conversion ratio than low-FE birds (0.18 g/g). High-FE birds showed greater IGF-I mRNA expression in the liver and muscle and greater GHR mRNA expression in the muscle. 4. Environmental effects only affected GHR expression in the liver, with quails under comfortable conditions exhibiting greater GHR expression than quails subjected to HS. 5. There was a significant interaction between FE and environmental temperature on ANT mRNA expression in the liver. The greatest ANT mRNA expression was observed for high FE-birds that remained under comfortable conditions. 6. In the liver, UCP mRNA expression did not differ among the quails and was not affected by environment or efficiency. However, comparisons of the low- and high-FE birds revealed higher levels of UCP mRNA in the muscle of low-FE birds. 7. COX III mRNA expression in the liver was dependent on environmental temperature and FE. Higher COX III mRNA expression was observed in animals that remained under comfortable conditions, and high-FE birds exhibited higher expression levels compared to low-FE birds. 8. These results suggest a correlation between IGF-I, GHR, ANT, UCP and COX III gene expression and FE and that environmental temperature could affect the expression of some of these genes.


Asunto(s)
Proteínas Aviares/genética , Coturnix/fisiología , Regulación de la Expresión Génica , Respuesta al Choque Térmico , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Proteínas Aviares/metabolismo , Coturnix/genética , Dieta , Femenino , Hígado/metabolismo , Músculo Esquelético/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo
14.
Phys Chem Chem Phys ; 15(39): 16542-56, 2013 Oct 21.
Artículo en Inglés | MEDLINE | ID: mdl-23949215

RESUMEN

A transferable potential energy function for describing the interaction between water molecules is presented. The electrostatic interaction is described rigorously using a multipole expansion. Only one expansion center is used per molecule to avoid the introduction of monopoles. This single center approach turns out to converge and give close agreement with ab initio calculations when carried out up to and including the hexadecapole. Both dipole and quadrupole polarizability are included. All parameters in the electrostatic interaction as well as the dispersion interaction are taken from ab initio calculations or experimental measurements of a single water molecule. The repulsive part of the interaction is parametrized to fit ab initio calculations of small water clusters and experimental measurements of ice Ih. The parametrized potential function was then used to simulate liquid water and the results agree well with experiment, even better than simulations using some of the point charge potentials fitted to liquid water. The evaluation of the new interaction potential for condensed phases is fast because point charges are not present and the interaction can, to a good approximation, be truncated at a finite range.


Asunto(s)
Hielo , Agua/química , Dimerización , Modelos Moleculares , Electricidad Estática
15.
PLoS One ; 18(10): e0287063, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37831658

RESUMEN

The publication of tourism statistics often does not keep up with the highly dynamic tourism demand trends, especially critical during crises. Alternative data sources such as digital traces and web searches represent an important source to potentially fill this gap, since they are generally timely, and available at detailed spatial scale. In this study we explore the potential of human mobility data from the Google Community Mobility Reports to nowcast the number of monthly nights spent at sub-national scale across 11 European countries in 2020, 2021, and the first half of 2022. Using a machine learning implementation, we found that this novel data source is able to predict the tourism demand with high accuracy, and we compare its potential in the tourism domain to web search and mobile phone data. This result paves the way for a more frequent and timely production of tourism statistics by researchers and statistical entities, and their usage to support tourism monitoring and management, although privacy and surveillance concerns still hinder an actual data innovation transition.


Asunto(s)
Turismo , Humanos , Europa (Continente)
16.
Braz J Med Biol Res ; 56: e12391, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37851789

RESUMEN

Rupture of Achilles tendon is a common accident affecting professional and recreational athletes. Acute and chronic pain are symptoms commonly observed in patients with rupture. However, few studies have investigated whether Achilles tendon rupture is able to promote disorders in the central nervous system (CNS). Therefore, the current study aimed to evaluate nociceptive alterations and inflammatory response in the L5 lumbar segment of Balb/c mice spinal cord after Achilles tendon rupture. We found increased algesia in the paw of the ruptured group on the 7th and 14th days post-tenotomy compared with the control group. This phenomenon was accompanied by overexpression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase-2 (NOS-2) as well as hyperactivation of astrocytes and microglia in nociceptive areas of L5 spinal cord as evidenced by intense GFAP and IBA-1 immunostaining, respectively. Biochemical studies also demonstrated increased levels of nitrite in the L5 spinal cord of tenotomized animals compared with the control group. Thus, we have demonstrated for the first time that total rupture of the Achilles tendon induced inflammatory response and nitrergic and glial activation in the CNS in the L5 spinal cord region.


Asunto(s)
Tendón Calcáneo , Humanos , Ratones , Animales , Médula Espinal , Astrocitos , Microglía , Tenotomía
17.
Genet Mol Res ; 11(3): 3063-8, 2012 Aug 31.
Artículo en Inglés | MEDLINE | ID: mdl-23007983

RESUMEN

Glycerol can be used as a substitute for corn for feeding poultry, but there are concerns about how it may affect growth performance and health of the birds. We evaluated the expression of mRNA of growth hormone (GH) and insulin-like growth factor I (IGF-I) in 35-day-old Japanese quails fed different glycerol levels (0, 4, and 8% dietary glycerol instead of corn). Total RNA was extracted from the breast muscle and cDNA was amplified with the use of specific primers for these genes using real-time PCR. Quails fed the diet with 8% glycerol supplementation had significantly lower GH mRNA and IGF-I mRNA expression than those fed no glycerol or 4% glycerol. No significant effect of the treatments was found on quail weight gain or feed intake. Feed conversion ratio was influenced by dietary glycerol levels: the group fed 8% glycerol displayed the worst feed conversion ratio (2.54) compared with that of quail fed the control diet (2.35) or 4% glycerol (2.36). Considering quail performance and the expression of the genes GH and IGF-I, a level of 4% glycerol can be used in quail feeding without any harmful effects.


Asunto(s)
Coturnix/crecimiento & desarrollo , Coturnix/genética , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Glicerol/farmacología , Hormona del Crecimiento/genética , Factor I del Crecimiento Similar a la Insulina/genética , Animales , Dieta , Conducta Alimentaria/efectos de los fármacos , Hormona del Crecimiento/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Aumento de Peso/efectos de los fármacos , Aumento de Peso/genética
18.
Br Poult Sci ; 53(4): 497-507, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23130585

RESUMEN

1. A study was carried out to evaluate the expression of growth hormone (GH), insulin-like growth factor I (IGF-I), mitochondrial adenine nucleotide translocase (ANT), cytochrome oxidase III (COX III) and avian uncoupling protein (avUCP) genes in 14-d-old Japanese quail that were fed different levels of glycerol (0%, 4%, and 12% dietary glycerol) which replaced maize as an energy source. 2. Total RNA was extracted from the breast muscle, and cDNA was amplified using real-time PCR with primers specific to the examined genes. 3. Quail fed the diet with 12% glycerol supplementation presented higher growth hormone (GH) mRNA expression than did those fed 0% glycerol. Supplementation with 12% glycerol negatively influenced IGF-I mRNA expression and reduced ANT mRNA expression in comparison with the treatment with no glycerol. COX III mRNA expression in the pectoralis superficialis muscle was decreased by 26% in quail fed 12% glycerol compared with those fed 0 and 4% glycerol. 4. There was no difference in UCP mRNA expression between quail fed 0 and 4% glycerol; however, UCP expression was reduced (73%) in birds fed the 12% glycerol diet compared with the level in those fed the 4% glycerol diet. 5. The inclusion of 4% glycerol in the diet produced results similar to those of the diet with no glycerol. Based on quail performance and the expression of the GH, IGF-I, ANT, COX III and UCP genes, 4% glycerol can be used in quail feeding without any harmful effects.


Asunto(s)
Coturnix/crecimiento & desarrollo , Coturnix/genética , Suplementos Dietéticos/análisis , Regulación de la Expresión Génica/efectos de los fármacos , Glicerol/farmacología , Músculos Pectorales/metabolismo , Alimentación Animal , Animales , Proteínas Aviares/genética , Proteínas Aviares/metabolismo , ADN Complementario/metabolismo , Relación Dosis-Respuesta a Droga , Glicerol/administración & dosificación , Hormona del Crecimiento/genética , Hormona del Crecimiento/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Distribución Aleatoria , Reacción en Cadena en Tiempo Real de la Polimerasa , Factores de Tiempo
19.
Cien Saude Colet ; 27(12): 4371-4380, 2022 Dec.
Artículo en Portugués, Inglés | MEDLINE | ID: mdl-36383850

RESUMEN

A document containing a prison policy management model was examined during a study of the governmental categories established to address confinement in Brazil. Based on Bourdieu and Hertz, an attempt was made to develop an ethnography attentive to the expressions used to refer to populations and institutions so that they could reveal ongoing terminological changes and maintenance. Deprivation of liberty is traversed by public policies and vice versa, pointing to prisons as "intersectoral spaces" and prisoners as "people deprived of liberty".


Ao longo de um estudo sobre as categorias governamentais criadas para abordar o fenômeno do confinamento no Brasil, um documento contendo um modelo de gestão da política prisional foi examinado. Com base em Bourdieu e Hertz, procurou-se desenvolver uma etnografia atenta às expressões para se referir a populações e estabelecimentos, de maneira que possam revelar mudanças e manutenções terminológicas em curso. Nota-se que a privação de liberdade é atravessada pelas políticas públicas e vice-versa, apontando os cárceres como "espaços intersetoriais" e os presos como "pessoas em privação de liberdade".


Asunto(s)
Prisioneros , Prisiones , Humanos , Política de Salud , Brasil
20.
Cien Saude Colet ; 27(1): 111-121, 2022 Jan.
Artículo en Portugués, Inglés | MEDLINE | ID: mdl-35043891

RESUMEN

A fundamental government initiative to change the living conditions of deinstitutionalized Brazilian people, the De Volta para Casa (Back Home) Program is formed by some elements, including the psychosocial rehabilitation financial aid. Thirty-four state and academic documents from ordinances to papers were gathered to identify which elements of the Program permeate the records and debates about it throughout the Brazilian Psychiatric Reform, converging with the studies of Juarez Pereira Furtado and Ligia Maria Vieira-da-Silva about the dynamics of scientific and bureaucratic fields, such as mental health. Furthermore, we ethnographed five events that gathered institutionalized people and other agents from this field. We noticed the remarkable presence of the Therapeutic Residential Services (SRT) as an element of the referred Program both in the records and the scientific debates, while the mentioned financial aid was highlighted mainly in the government records.


O Programa de Volta para Casa, iniciativa governamental fundamental para modificar as condições de vida de egressos de internação psiquiátrica no Brasil, é formado por alguns componentes, entre eles o auxílio-reabilitação psicossocial. Com o objetivo de identificar quais componentes do referido Programa estão presentes nos registros e debates sobre ele ao longo do processo de Reforma Psiquiátrica Brasileira, 34 documentos foram reunidos, de portarias a artigos, em convergência com os estudos de Juarez Pereira Furtado e Ligia Maria Vieira-da-Silva sobre a dinâmica de campos ao mesmo tempo científicos e burocráticos, como o da saúde mental. Além disso, cinco eventos que reuniam egressos de internação psiquiátrica e outros agentes desse campo foram etnografados. Notamos a presença marcante do Serviço Residencial Terapêutico como componente do referido Programa nos registros e debates científicos, enquanto o mencionado auxílio foi destacado principalmente nos registros governamentais.


Asunto(s)
Desinstitucionalización , Servicios de Salud Mental , Pobreza , Brasil , Humanos , Servicios de Salud Mental/economía
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