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1.
Clin Genet ; 83(1): 73-7, 2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22335494

RESUMEN

Pitt-Hopkins syndrome (PTHS) is a neurodevelopmental disorder characterized by intellectual disability, unusual face and breathing abnormalities and can be caused by haploinsufficiency of TCF4. The majority of cases are sporadic. Somatic mosaicism was reported infrequently. We report on a proband with typical manifestations of PTHS and his younger brother with a less striking phenotype. In both, a heterozygous frameshift mutation (c.1901_1909delinsA, p.Ala634AspfsX67) was found in exon 19 of TCF4. The same mutation was found at low levels in DNA extracted from the mother's blood, urine and saliva. This report of familial recurrence with somatic mosaicism in a healthy mother has important consequences for genetic counseling. We suggest careful studies in parents of other patients with PTHS to determine the frequency of germline and somatic mosaicism for TCF4 mutations.


Asunto(s)
Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice/genética , Hiperventilación/genética , Discapacidad Intelectual/genética , Mosaicismo , Factores de Transcripción/genética , Adulto , Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice/sangre , Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice/orina , Niño , Preescolar , Facies , Femenino , Mutación del Sistema de Lectura , Asesoramiento Genético , Haploinsuficiencia/genética , Humanos , Hiperventilación/sangre , Hiperventilación/diagnóstico , Hiperventilación/orina , Discapacidad Intelectual/sangre , Discapacidad Intelectual/diagnóstico , Discapacidad Intelectual/orina , Masculino , Madres , Fenotipo , Factor de Transcripción 4 , Factores de Transcripción/sangre , Factores de Transcripción/orina
2.
Hum Reprod ; 28(8): 2308-11, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23760159

RESUMEN

STUDY QUESTION: Can we confirm in our population whether FMRI low sub-genotypes are associated with BRCA1/2 mutations, as recently proposed? SUMMARY ANSWER: Our results indicate that the distribution of the FMR1 sub-genotypes in female BRCA1/2-mutation carriers is significantly different from what has been reported previously and resembles that of the control population. FMRI low sub-genotypes are not associated with BRCA1/2 mutations and this association is also absent among male mutation carriers. WHAT IS KNOWN ALREADY: Recently, BRCA1 mutations were reported to be associated with primary ovarian insufficiency (POI) in female carriers. In animal models, BRCA2-deficiency also results in impaired oogenesis. A recent study has reported that the POI in BRCA1/2-mutation carriers is most likely due to low FMR1 sub-genotype (CGG n < 26) and the authors also suggested that low sub-genotypes of the FMR1 gene might be important to rescue the BRCA1/2 embryos, which would otherwise be embryonically-lethal. STUDY DESIGN, SIZE, DURATION: This retrospective study was performed in October and November of 2012, using genetic material of 464 patients who underwent genetic screening in our centre in the past. PARTICIPANTS/MATERIALS, SETTING, METHODS: We tested the FMR1 sub-genotypes in 60 female and 29 males with either BRCA1 or BRCA2 mutations and 375 controls by PCR amplification and size fragment analysis. MAIN RESULTS: We did not find any evidence for an association of FMR1 low sub-genotypes and BRCA1/2 mutations. LIMITATIONS, REASONS FOR CAUTION: This association study assumes that the female BRCA1/2 population tested has POI. WIDER IMPLICATIONS OF THE FINDINGS: Low FMR1 sub-genotypes are not responsible for the presumed rescue of embryos with BRCA1/2 mutations. Furthermore, the molecular mechanism of the POI in BRCA1/2-female carriers is not likely to be associated with low FMR1 sub-genotype. STUDY FUNDING/COMPETING INTEREST(S): The Department of Clinical Genetics of the Maastricht University Medical Centre supported the study. The authors do not have any competing interests to declare.


Asunto(s)
Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil/genética , Genes BRCA1 , Genes BRCA2 , Genotipo , Heterocigoto , Insuficiencia Ovárica Primaria/genética , Embrión de Mamíferos , Femenino , Estudios de Asociación Genética , Humanos , Masculino , Mutación , Diagnóstico Preimplantación , Estudios Retrospectivos
3.
Br J Cancer ; 106(12): 2016-24, 2012 Jun 05.
Artículo en Inglés | MEDLINE | ID: mdl-22669161

RESUMEN

BACKGROUND: The variable penetrance of breast cancer in BRCA1/2 mutation carriers suggests that other genetic or environmental factors modify breast cancer risk. Two genes of special interest are prohibitin (PHB) and methylene-tetrahydrofolate reductase (MTHFR), both of which are important either directly or indirectly in maintaining genomic integrity. METHODS: To evaluate the potential role of genetic variants within PHB and MTHFR in breast and ovarian cancer risk, 4102 BRCA1 and 2093 BRCA2 mutation carriers, and 6211 BRCA1 and 2902 BRCA2 carriers from the Consortium of Investigators of Modifiers of BRCA1 and BRCA2 (CIMBA) were genotyped for the PHB 1630 C>T (rs6917) polymorphism and the MTHFR 677 C>T (rs1801133) polymorphism, respectively. RESULTS: There was no evidence of association between the PHB 1630 C>T and MTHFR 677 C>T polymorphisms with either disease for BRCA1 or BRCA2 mutation carriers when breast and ovarian cancer associations were evaluated separately. Analysis that evaluated associations for breast and ovarian cancer simultaneously showed some evidence that BRCA1 mutation carriers who had the rare homozygote genotype (TT) of the PHB 1630 C>T polymorphism were at increased risk of both breast and ovarian cancer (HR 1.50, 95%CI 1.10-2.04 and HR 2.16, 95%CI 1.24-3.76, respectively). However, there was no evidence of association under a multiplicative model for the effect of each minor allele. CONCLUSION: The PHB 1630TT genotype may modify breast and ovarian cancer risks in BRCA1 mutation carriers. This association need to be evaluated in larger series of BRCA1 mutation carriers.


Asunto(s)
Neoplasias de la Mama/genética , Genes BRCA1 , Genes BRCA2 , Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Neoplasias Ováricas/genética , Polimorfismo Genético , Proteínas Represoras/genética , Femenino , Predisposición Genética a la Enfermedad , Heterocigoto , Humanos , Mutación , Prohibitinas , Riesgo
4.
Breast Cancer Res Treat ; 133(1): 393-8, 2012 May.
Artículo en Inglés | MEDLINE | ID: mdl-22370629

RESUMEN

Despite extensive analysis of the BRCA1 and BRCA2 genes, germline mutations are detected in <20% of families with a presumed genetic predisposition for breast and ovarian cancer. Recent literature reported RAD51C as a new breast cancer susceptibility gene. In this study, we report the analysis of 410 patients from 351 unrelated pedigrees. All were referred for genetic testing and we selected families with at least one reported case of ovarian cancer in which BRCA1&2 mutations were previously ruled out. We analyzed the coding exons, intron-exons boundaries, and UTRs of RAD51C. Our mutation analysis did not reveal any unequivocal deleterious mutation. In total 12 unique sequence variations were identified of which two were novel. Our study and others suggest a low prevalence of RAD51C mutations with an exception for some founder populations. This observation is in favor of the rare allele hypothesis in the debate over the nature of the genetic contribution to individual susceptibility to breast and ovarian cancer and further genome-wide studies in high risk families are warranted.


Asunto(s)
Proteínas de Unión al ADN/genética , Síndrome de Cáncer de Mama y Ovario Hereditario/genética , Análisis Mutacional de ADN , Femenino , Frecuencia de los Genes , Estudios de Asociación Genética , Predisposición Genética a la Enfermedad , Pruebas Genéticas , Humanos , Masculino , Mutación Missense , Linaje , Polimorfismo de Nucleótido Simple
5.
NPJ Genom Med ; 6(1): 95, 2021 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-34782607

RESUMEN

Neurofibromatosis type 1 (NF1) is caused by loss-of-function variants in the NF1 gene. Approximately 10% of these variants affect RNA splicing and are either missed by conventional DNA diagnostics or are misinterpreted by in silico splicing predictions. Therefore, a targeted RNAseq-based approach was designed to detect pathogenic RNA splicing and associated pathogenic DNA variants. For this method RNA was extracted from lymphocytes, followed by targeted RNAseq. Next, an in-house developed tool (QURNAs) was used to calculate the enrichment score (ERS) for each splicing event. This method was thoroughly tested using two different patient cohorts with known pathogenic splice-variants in NF1. In both cohorts all 56 normal reference transcript exon splice junctions, 24 previously described and 45 novel non-reference splicing events were detected. Additionally, all expected pathogenic splice-variants were detected. Eleven patients with NF1 symptoms were subsequently tested, three of which have a known NF1 DNA variant with a putative effect on RNA splicing. This effect could be confirmed for all 3. The other eight patients were previously without any molecular confirmation of their NF1-diagnosis. A deep-intronic pathogenic splice variant could now be identified for two of them (25%). These results suggest that targeted RNAseq can be successfully used to detect pathogenic RNA splicing variants in NF1.

6.
J Med Genet ; 46(11): 776-85, 2009 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19578034

RESUMEN

BACKGROUND: Mutations in the DNA polymerase-gamma (POLG) gene are a major cause of clinically heterogeneous mitochondrial diseases, associated with mtDNA depletion and multiple deletions. OBJECTIVE: To determine the spectrum of POLG mutations in our Dutch patient cohort, to evaluate the pathogenicity of novel mutations, and to establish genotype-phenotype correlations. RESULTS: The authors identified 64 predominantly recessive mutations in 37 patients from a total of 232 patients, consisting of 23 different mutations. The substitution p.A467T was most frequently observed (n = 23), but was as frequent in childhood cases as in adult cases. Five new pathogenic recessive mutations, p.Lys925ArgfsX42, p.R275X, p.G426S, p.A804T and p.R869Q were identified. The known dominant chronic progressive external ophthalmoplegia (CPEO) mutation p.R943H was for the first time associated with premature ovarian failure as well. In 19 patients the authors identified only a single recessive mutation, or a sequence variant with unclear clinical significance. The data substantiate earlier observations that in POLG patients a fatal status epilepticus and liver failure can be triggered by sodium valproate. It is therefore important to exclude POLG mutations before administering this treatment. CONCLUSION: The clinical features of the patient are the most important features to select putative POLG mutation carriers and not the presence of mtDNA deletions or OXPHOS (oxidative phosphorylation) activity. The authors conclude that POLG mutations are an important cause of heterogeneous mitochondrial pathology and that more accurate genotype-phenotype correlations allow a more rapid genetic diagnosis and improved prognosis for mutation carriers.


Asunto(s)
ADN Polimerasa Dirigida por ADN/genética , Mutación , Adolescente , Adulto , Anciano , Secuencia de Aminoácidos , Niño , Preescolar , Estudios de Cohortes , Simulación por Computador , Análisis Mutacional de ADN , ADN Polimerasa gamma , ADN Mitocondrial/genética , Femenino , Frecuencia de los Genes , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Oftalmoplejía Externa Progresiva Crónica/genética , Linaje , Fenotipo , Insuficiencia Ovárica Primaria/genética , Alineación de Secuencia
7.
Fam Cancer ; 18(2): 273-280, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30607672

RESUMEN

Early-onset breast cancer may be due to Li-Fraumeni Syndrome (LFS). Current national and international guidelines recommend that TP53 genetic testing should be considered for women with breast cancer diagnosed before the age of 31 years. However, large studies investigating TP53 mutation prevalence in this population are scarce. We collected nationwide laboratory records for all young breast cancer patients tested for TP53 mutations in the Netherlands. Between 2005 and 2016, 370 women diagnosed with breast cancer younger than 30 years of age were tested for TP53 germline mutations, and eight (2.2%) were found to carry a (likely) pathogenic TP53 sequence variant. Among BRCA1/BRCA2 mutation negative women without a family history suggestive of LFS or a personal history of multiple LFS-related tumours, the TP53 mutation frequency was < 1% (2/233). Taking into consideration that TP53 mutation prevalence was comparable or even higher in some studies selecting patients with breast cancer onset at older ages or HER2-positive breast cancers, raises the question of whether a very early age of onset is an appropriate single TP53 genetic testing criterion.


Asunto(s)
Neoplasias de la Mama/genética , Asesoramiento Genético/normas , Pruebas Genéticas/normas , Síndrome de Li-Fraumeni/genética , Proteína p53 Supresora de Tumor/genética , Adolescente , Adulto , Factores de Edad , Edad de Inicio , Proteína BRCA1/genética , Proteína BRCA2/genética , Neoplasias de la Mama/diagnóstico , Neoplasias de la Mama/epidemiología , Análisis Mutacional de ADN , Femenino , Asesoramiento Genético/estadística & datos numéricos , Predisposición Genética a la Enfermedad , Pruebas Genéticas/estadística & datos numéricos , Mutación de Línea Germinal , Humanos , Síndrome de Li-Fraumeni/diagnóstico , Síndrome de Li-Fraumeni/epidemiología , Anamnesis , Países Bajos/epidemiología , Guías de Práctica Clínica como Asunto , Estudios Retrospectivos , Adulto Joven
8.
J Med Genet ; 44(4): e74, 2007 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-17400793

RESUMEN

BACKGROUND: Detection of mutations in the mitochondrial DNA (mtDNA) is usually limited to common mutations and the transfer RNA genes. However, mutations in other mtDNA regions can be an important cause of oxidative phosphorylation (OXPHOS) disease as well. OBJECTIVE: To investigate whether regions in the mtDNA are preferentially mutated in patients with OXPHOS disease. METHODS: Screening of the mtDNA for heteroplasmic mutations was performed by denaturing high-performance liquid chromatography analysis of 116 patients with OXPHOS disease but without the common mtDNA mutations. RESULTS: An mtDNA sequence variant was detected in 15 patients, 5 of which were present in the ND5 gene. One sequence variant was new and three were known, one of which was found twice. The novel sequence variant m.13511A-->T occurred in a patient with a Leigh-like syndrome. The known mutation m.13513G-->A, associated with mitochondrial encephalomyopathy lactic acidosis and stroke-like syndrome (MELAS) and MELAS/Leigh/Leber hereditary optic neuropathy overlap syndrome, was found in a relatively low percentage in two patients from two different families, one with a MELAS/Leigh phenotype and one with a MELAS/chronic progressive external ophthalmoplegia phenotype. The known mutation m.13042G-->A, detected previously in a patient with a MELAS/myoclonic epilepsy, ragged red fibres phenotype and in a family with a prevalent ocular phenotype, was now found in a patient with a Leigh-like phenotype. The sequence variant m.12622G-->A was reported once in a control database as a polymorphism, but is reported in this paper as heteroplasmic in three brothers, all with infantile encephalopathy (Leigh syndrome) fatal within the first 15 days of life. Therefore, a causal relationship between the presence of this sequence variant and the onset of mitochondrial disease cannot be entirely excluded at this moment. CONCLUSIONS: Mutation screening of the ND5 gene is advised for routine diagnostics of patients with OXPHOS disease, especially for those with MELAS- and Leigh-like syndrome with a complex I deficiency.


Asunto(s)
Complejo I de Transporte de Electrón/genética , Enfermedades Mitocondriales/genética , Proteínas Mitocondriales/genética , Fosforilación Oxidativa , Secuencia de Aminoácidos , Animales , Encéfalo/anomalías , Niño , Cromatografía Líquida de Alta Presión , Análisis Mutacional de ADN , ADN Mitocondrial/genética , Enfermedades en Gemelos , Complejo I de Transporte de Electrón/química , Complejo I de Transporte de Electrón/deficiencia , Complejo I de Transporte de Electrón/fisiología , Resultado Fatal , Femenino , Pruebas Genéticas , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Recién Nacido , Enfermedad de Leigh/genética , Síndrome MELAS/genética , Masculino , Mitocondrias Musculares/enzimología , Proteínas Mitocondriales/química , Proteínas Mitocondriales/deficiencia , Proteínas Mitocondriales/fisiología , Datos de Secuencia Molecular , Mutación Missense , Fenotipo , Subunidades de Proteína , Alineación de Secuencia , Homología de Secuencia de Aminoácido
9.
Transplantation ; 67(9): 1274-7, 1999 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-10342321

RESUMEN

BACKGROUND: The early detection of human cytomegalovirus infection after organ transplantation is a prerequisite for effective antiviral therapy. We evaluated the diagnostic value of monitoring the viral immediate-early (IE) 1 mRNA expression in blood leukocytes by nucleic acid sequence-based amplification (NASBA). METHODS: Nucleic acids were isolated from 489 blood samples collected from 42 kidney transplant recipients and subjected to amplification by IE NASBA. The IE NASBA results were compared to those from pp67 NASBA, pp65 antigenemia, cell culture (DEAFF and CPE), and serology. RESULTS: IE NASBA proved to be the most sensitive assay which detected the onset of both primary and secondary cytomegalovirus infection significantly earlier than the other assays. CONCLUSIONS: The early detection of cytomegalovirus infection with IE NASBA would enable the start of effective antiviral therapy at an early state of infection to prevent cytomegalovirus disease in patients at risk.


Asunto(s)
Infecciones por Citomegalovirus/diagnóstico , Citomegalovirus , Amplificación de Genes , Trasplante de Riñón/efectos adversos , Ácidos Nucleicos/sangre , Infecciones por Citomegalovirus/sangre , Infecciones por Citomegalovirus/prevención & control , ADN Viral/sangre , Humanos , Proteínas Inmediatas-Precoces/biosíntesis , Estudios Prospectivos , ARN Mensajero/biosíntesis , ARN Mensajero/sangre , Sensibilidad y Especificidad , Análisis de Secuencia de ADN/métodos , Proteínas Virales/biosíntesis
10.
Transplantation ; 71(9): 1257-61, 2001 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-11397959

RESUMEN

BACKGROUND: Cytomegalovirus (CMV) infection has been linked to acute and chronic rejection. We have previously shown that concomitant rat cytomegalovirus (RCMV) infection increases portal inflammation and bile duct destruction in rejecting rat liver allografts. Many of the pro-inflammatory effects of CMV have been attributed to the immediate early (IE) proteins of CMV. We wanted to investigate whether RCMV and IE-1 gene expression persist in the liver graft in our model. METHODS: Liver transplantations were performed from PVG (RT1c) into BN (RT1n) rats. One day after transplantation, the rats were infected with RCMV. No immunosuppression was given. The graft infection was studied by viral culture, immunofluorescence, DNA in situ hybridization and RT-PCR for the detection of IE-1 mRNA at various time points. RESULTS: RCMV caused an active infection from 5 days to 2 weeks after transplantation, during which infectious virus was found in the graft. Thereafter the cultures were negative. RCMV antigens and DNA were found in hepatocytes, endothelial, inflammatory, and bile duct cells during the active infection. At 4 weeks, RCMV DNA positive hepatocytes, endothelial, inflamma tory, and bile duct cells could still be found, but in much smaller quantities. IE-1 mRNA expression was, however, only detected during the active infection, not at 4 weeks postinfection. CONCLUSIONS: RCMV IE-1 expression does not persist in the graft after the active infection, although some viral DNA can be detected in the graft up to 4 weeks. In our model, the CMV-induced increase in graft damage does not seem to require the continued expression of IE-1.


Asunto(s)
Infecciones por Citomegalovirus , Citomegalovirus/genética , ADN Viral/metabolismo , Genes Inmediatos-Precoces/genética , Genes Virales/genética , Trasplante de Hígado/inmunología , Animales , Antígenos Virales/análisis , Citomegalovirus/inmunología , Infecciones por Citomegalovirus/complicaciones , Efecto Citopatogénico Viral , Expresión Génica , Rechazo de Injerto/genética , Rechazo de Injerto/virología , Hibridación in Situ , ARN Mensajero/metabolismo , Ratas , Ratas Endogámicas BN
11.
Fam Cancer ; 9(2): 193-201, 2010 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-19949876

RESUMEN

Considerable differences exist amongst countries in the mutation probability methods and thresholds used to select patients for BRCA1/2 genetic screening. In order to assess the added value of mutation probability methods, we have retrospectively calculated the BRCAPRO and Myriad II probabilities in 306 probands who had previously been selected for DNA-analysis according to criteria based on familial history of cancer. DNA-analysis identified 52 mutations (16.9%) and 11 unclassified variants (UVs, 3.6%). Compared to cancer history, a threshold > or = 10% with BRCAPRO or with Myriad II excluded about 40% of the patients from analysis, including four with a mutation and probabilities <10% with both programs. All four probands had a BRCA2 mutation. BRCAPRO and Myriad II showed similar specificity at 10% threshold, overall BRCAPRO was more sensitive than Myriad II for the detection of mutations. Only two of the probands with an UV had probabilities >20% with BRCAPRO and Myriad II. In summary, BRCAPRO and Myriad II are more efficient than cancer history alone to exclude patients without a mutation. BRCAPRO performs better for the detection of BRCA1 mutations than of BRCA2 mutations. The Myriad II scores provided no additional information than the BRCAPRO scores alone for the detection of patients with a mutation. The use of thresholds excluded from analysis the majority of patients carrying an UV.


Asunto(s)
Neoplasias de la Mama/diagnóstico , Tamización de Portadores Genéticos/métodos , Predisposición Genética a la Enfermedad/epidemiología , Pruebas Genéticas/métodos , Linaje , Algoritmos , Proteína BRCA1/genética , Neoplasias de la Mama/epidemiología , Femenino , Genes BRCA1/fisiología , Asesoramiento Genético/métodos , Variación Genética , Humanos , Masculino , Mutación , Probabilidad
15.
Arch Virol ; 150(12): 2593-9, 2005 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-16052287

RESUMEN

We have identified and characterized two antisense transcripts from the rat cytomegalovirus (RCMV) major immediate early (MIE) region. These transcripts, designated IE-AS1 and IE-AS2, are complementary to part of the sense IE1 transcript. The IE-AS transcripts were first detected in peripheral blood leukocytes (PBL) of RCMV-infected rats at 7 days post-infection (pi) in the absence of IE1 transcription. Nevertheless, both the IE1 and IE-AS transcripts were found at the same time in the salivary glands of RCMV-infected rats at 7 and 120 days pi as well as in RCMV-infected rat embryo fibroblasts (REFs) at 48 h pi.


Asunto(s)
Antígenos Virales/genética , Proteínas Inmediatas-Precoces/genética , Muromegalovirus/genética , ARN sin Sentido/análisis , ARN Mensajero/análisis , ARN Viral/análisis , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Fibroblastos/virología , Leucocitos/virología , Datos de Secuencia Molecular , ARN sin Sentido/genética , ARN Complementario/genética , ARN Mensajero/genética , ARN Viral/genética , Ratas , Glándulas Salivales/virología
16.
Virology ; 227(1): 189-97, 1997 Jan 06.
Artículo en Inglés | MEDLINE | ID: mdl-9007072

RESUMEN

A reconstituted transcription system composed of the Rift Valley fever phlebovirus (Bunyaviridae family) proteins L and N expressed via recombinant vaccinia viruses and an S-like model RNA containing the CAT gene in the antisense orientation, has been described previously by Lopez et al. (J. Virol., 1995, 69, 3972-3979). We extended the use of this in vivo system to determine the sequence at the 3' end of the ambisense S segment recognized by the transcription complex. A mutational analysis of the sequences at the 3' end of the S-like genomic or antigenomic RNA was undertaken. The data indicated that the minimal sequence required for transcription resides in the 13 first 3' nucleotides of the genomic or antigenomic RNA. In these sequences, two regions appeared crucial: the bases at positions 3 to 8 and the purine at position 13. In addition, the terminal repeat ...GU could be deleted without affecting significantly the template activity of the RNA. These data support the prime and realign mechanism proposed recently for Bunya- and Arenaviruses


Asunto(s)
Regulación Viral de la Expresión Génica , Nucleoproteínas/genética , ARN Viral/genética , Secuencias Reguladoras de Ácidos Nucleicos , Virus de la Fiebre del Valle del Rift/genética , Proteínas no Estructurales Virales/genética , Proteínas Virales/genética , Cloranfenicol O-Acetiltransferasa/genética , Células HeLa , Humanos , Moldes Genéticos , Transcripción Genética
17.
J Clin Microbiol ; 38(12): 4485-91, 2000 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-11101584

RESUMEN

Nucleic acid sequence-based amplification (NASBA) was used for detection of the human cytomegalovirus (CMV) immediate early-1 (IE) and the late pp67 mRNA in 353 blood samples collected from 34 liver transplant patients. The diagnostic value of these assays was compared to that of the pp65 antigenemia assay. Overall, 95 and 42% of the antigenemia-positive samples were IE NASBA and pp67 NASBA positive, respectively. Although the results from pp67 NASBA and the antigenemia assay appeared to correspond poorly, a clear correlation was seen between pp67 NASBA-negative results and low numbers of pp65 antigen-positive cells. Twenty patients (59%) were treated with ganciclovir after the diagnosis of symptomatic CMV infection. Before initiation of the antiviral therapy, the antigenemia assay detected the onset of symptomatic infection in all patients, whereas 95 and 60% of these patients were IE NASBA and pp67 NASBA positive, respectively. Although the sensitivity of IE NASBA was very high, the positive predictive value (PPV) of this assay for the onset of a symptomatic infection was only 63%. The PPV of the antigenemia assay as well as pp67 NASBA was considerably higher (80 and 86%, respectively). Thus, the detection of IE mRNA using NASBA appears to be particularly useful as a marker for early initiation of antiviral therapy in patients at high risk for the development of a symptomatic infection. Also, IE NASBA was found to be more sensitive than the antigenemia assay for monitoring CMV infection during antiviral therapy. On the contrary, pp67 NASBA did not appear to have additional diagnostic value compared to the antigenemia assay.


Asunto(s)
Antígenos Virales/genética , Citomegalovirus/aislamiento & purificación , Amplificación de Genes , Proteínas Inmediatas-Precoces/genética , Trasplante de Hígado/efectos adversos , ARN Mensajero/análisis , Proteínas Virales/genética , Viremia/virología , Adulto , Citomegalovirus/efectos de los fármacos , Ganciclovir/farmacología , Humanos , Fosfoproteínas/genética
18.
J Virol ; 74(2): 1045-50, 2000 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-10623772

RESUMEN

The rat cytomegalovirus (RCMV) r144 gene encodes a polypeptide homologous to major histocompatibility complex class I heavy chains. To study the role of r144 in virus replication, an RCMV r144 null mutant strain (RCMVDeltar144) was generated. This strain replicated with efficiency similar to that of wild-type (WT) RCMV in vitro. Additionally, WT RCMV and RCMVDeltar144 were found not to differ in their replication characteristics in vivo. First, the survival rate was similar among groups of immunosuppressed rats infected with either RCMVDeltar144 or WT RCMV. Second, the dissemination of virus did not differ in either RCMVDeltar144- or WT RCMV-infected, immunosuppressed rats, either in the acute phase of infection or approximately 1 year after infection. These data indicate that the RCMV r144 gene is essential neither for virus replication in the acute phase of infection nor for long-term infection in immunocompromised rats. Interestingly, in a local infection model in which footpads of immunosuppressed rats were inoculated with virus, a significantly higher number of infiltrating macrophage cells as well as of CD8(+) T cells was observed in WT RCMV-infected paws than in RCMVDeltar144-infected paws. This suggests that r144 might function in the interaction with these leukocytes in vivo.


Asunto(s)
Infecciones por Citomegalovirus/virología , Citomegalovirus/genética , Proteínas Virales/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Citomegalovirus/fisiología , Infecciones por Citomegalovirus/inmunología , ADN Viral , Genes MHC Clase I , Genes Virales , Humanos , Huésped Inmunocomprometido , Cadenas Pesadas de Inmunoglobulina/química , Cadenas Pesadas de Inmunoglobulina/genética , Datos de Secuencia Molecular , Mutagénesis , Ratas , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Factores de Tiempo , Proteínas Virales/fisiología , Replicación Viral/fisiología
19.
Arch Virol ; 147(4): 813-24, 2002 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-12038690

RESUMEN

We previously generated an RCMV strain in which the r144 gene, encoding a major histocompatibility complex class I homolog, had been deleted (RCMVdelta r144). To investigate the role of r144 during acute infection of neonatal rats, we infected three days-old neonatal rats with either RCMVdelta r144 or wild type (wt) RCMV and the presence of infectious virus as well as viral DNA in various organs was determined at either 3, 5 or 21 days p.i.. In addition, we assessed both type and number of inflammatory cells in these organs. Interestingly, a significantly lower concentration of infectious virus as well as viral DNA was found in spleens of RCMVdelta r144-infected rats than in those of wt RCMV-infected animals at 3 days p.i.. At the same time point, a significantly lower amount of infiltrating NK cells and monocytes/macrophages was seen in the spleens of RCMVdelta r144-infected rats than in spleens of rats infected with wt RCMV. At 21 days p.i., RCMVdelta r144-infected rats were found to have lower virus titers in the salivary glands than wt RCMV-infected animals. Significant differences between RCMVdelta r144- and wt RCMV-infected rats were detected neither at other time points nor at other sites. We conclude that after infection of neonatal rats, the replication of RCMVdelta r144 is severely restricted compared to wt RCMV.


Asunto(s)
Genes MHC Clase I/genética , Infecciones por Herpesviridae/virología , Muromegalovirus/genética , Proteínas Virales/genética , Enfermedad Aguda , Animales , Animales Recién Nacidos , Recuento de Células , ADN Viral/análisis , Modelos Animales de Enfermedad , Femenino , Eliminación de Gen , Infecciones por Herpesviridae/inmunología , Leucocitos/virología , Macrófagos/virología , Masculino , Muromegalovirus/química , Ratas , Glándulas Salivales/virología , Bazo/inmunología , Bazo/virología , Factores de Tiempo
20.
Transpl Int ; 11 Suppl 1: S107-9, 1998.
Artículo en Inglés | MEDLINE | ID: mdl-9664957

RESUMEN

Early detection of active cytomegalovirus (CMV) infection after organ transplantation is necessary to start effective antiviral treatment. In the present study, blood specimens of kidney transplant recipients (n = 38) were monitored for the expression of CMV immediate early (IE) and late (L) mRNA using nucleic acid sequence-based amplification (NASBA). Results were compared with virus isolation, pp65 antigenemia and serology. In patients developing active CMV infection, pp65 antigen and L mRNA were detected simultaneously. At the same time, positive cell culture results could be reported to the clinic. CMV was detected significantly earlier with IE NASBA than with the other assays. However, the specificity of IE NASBA is lower than that of antigenemia, late NASBA and cell culture. Early detection of IE mRNA is especially useful for patients at high risk of developing symptomatic CMV infection in order that early, adequate antiviral therapy may be started. Late NASBA can be used to monitor further development of CMV infection, comparable to antigenemia.


Asunto(s)
Infecciones por Citomegalovirus/diagnóstico , Trasplante de Riñón , Complicaciones Posoperatorias/diagnóstico , Complicaciones Posoperatorias/virología , Humanos , Valor Predictivo de las Pruebas , Sensibilidad y Especificidad , Factores de Tiempo
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