Inducing pluripotency in vitro: recent advances and highlights in induced pluripotent stem cells generation and pluripotency reprogramming.

Induced pluripotent stem cells (iPSCs) are considered patient-specific counterparts of embryonic stem cells as they originate from somatic cells after forced expression of pluripotency reprogramming factors Oct4, Sox2, Klf4 and c-Myc. iPSCs offer unprecedented opportunity for personalized cell therapies in regenerative medicine. In recent years, iPSC technology has undergone substantial improvement to overcome slow and inefficient reprogramming protocols, and to ensure clinical-grade iPSCs and their functional derivatives. Recent developments in iPSC technology include better reprogramming methods employing novel delivery systems such as non-integrating viral and non-viral vectors, and characterization of alternative reprogramming factors. Concurrently, small chemical molecules (inhibitors of specific signalling or epigenetic regulators) have become crucial to iPSC reprogramming; they have the ability to replace putative reprogramming factors and boost reprogramming processes. Moreover, common dietary supplements, such as vitamin C and antioxidants, when introduced into reprogramming media, have been found to improve genomic and epigenomic profiles of iPSCs. In this article, we review the most recent advances in the iPSC field and potent application of iPSCs, in terms of cell therapy and tissue engineering.

Paget's sarcoma in Tasmania.

Six proven cases of Paget's sarcoma occurred in Tasmania in the decade 1965 to 1974. The incidence was 0-16 cases per 100,000 population per year. The sex ratio was two male: one female. The average age was 61-2 years. All cases occurred in the pelvis or adjacent femur. Average time of survival after onset of symptoms was one year. Two patients had lymph node metastases. One patient had no pulmonary metastases; a further patient may have had no pulmonary metastases. All patients were born in Australia (State of Tasmania) of predominantly Anglo-Saxon stock.

Reliability of barium enema in detecting colonic neoplasia.

Three hundred and seven unselected patients were examined by double contrast barium enemas. Twenty-four patients (7.8%) had carcinomas; 11 patients (3.6%) had polyps; six patients (2%) had possible polyps, not confirmed by colonoscopy; and in four patients (1.3%) the radiological findings were misleading or wrong. No carcinoma was missed by barium enema X-ray examination, to be diagnosed by other means. Two patients had radiological lesions, not demonstrable endoscopically, but confirmed surgically. Extensive follow-up of 286 patients (93.2%) revealed only one subsequent case of carcinoma. The shortcomings of colonoscopy are briefly discussed. It is recommended that barium enema examination precede colonoscopy in the investigation of suspected large bowel neoplasia, and that both be used alternately to screen high-risk patients.

General model of microbial growth and decomposition in aquatic ecosystems.

A model capable of simulating freely suspended and attached decomposers, particulate organic matter, labile and refractory dissolved organic matter, inorganic nitrogen, and phosphate in the open-water portion of lakes is presented. Examples are given showing the utility of the model when coupled to the whole-ecosystem model CLEANER.