Chondrocyte deformation induces mitochondrial distortion and heterogeneous intracellular strain fields.

Chondrocyte mechanotransduction is poorly understood but may involve cell deformation and associated distortion of intracellular structures and organelles. This study quantifies the intracellular displacement and strain fields associated with chondrocyte deformation and in particular the distortion of the mitochondria network, which may have a role in mechanotransduction. Isolated articular chondrocytes were compressed in agarose constructs and simultaneously visualised using confocal microscopy. An optimised digital image correlation technique was developed to calculate the local intracellular displacement and strain fields using confocal images of fluorescently labelled mitochondria. The mitochondria formed a dynamic fibrous network or reticulum, which co-localised with microtubules and vimentin intermediate filaments. Cell deformation induced distortion of the mitochondria, which collapsed in the axis of compression with a resulting loss of volume. Compression generated heterogeneous intracellular strain fields indicating mechanical heterogeneity within the cytoplasm. The study provides evidence supporting the potential involvement of mitochondrial deformation in chondrocyte mechanotransduction, possibly involving strain-mediated release of reactive oxygen species. Furthermore the heterogeneous strain fields, which appear to be influenced by intracellular structure and organisation, may generate significant heterogeneity in mechanotransduction behaviour for cells subjected to identical levels of deformation.

Modelling Tumor Treating Fields for the treatment of lung-based tumors.

Tumor Treating Fields (TTFields), low-intensity electric fields in the frequency range of 100-500 kHz, exhibit antimitotic activity in cancer cells. TTFields were approved by the U. S. Food and Drug Administration for the treatment of recurrent glioblastoma in 2011. Preclinical evidence and pilot studies suggest that TTFields could be effective for treating certain types of lung cancer, and that treatment efficacy depends on the electric field intensity. To optimize TTFields delivery to the lungs, it is important to understand how TTFields distribute within the chest. Here we present simulations showing how TTFields are distributed in the thorax and torso, and demonstrate how the electric field distribution within the body can be controlled by personalizing the layout of the arrays used to deliver the field.

Computer-generated space-variant polarization elements with subwavelength metal stripes.

A novel method of performing two-dimensional space-variant polarization operations is presented. The method is based on determining the local direction and period of subwavelength metal-stripe gratings by use of vectorial optics to obtain any desired continuous polarization change. We demonstrate our approach with specific computer-generated space-variant polarization elements for laser radiation at 10.6mum. The polarization properties are verified with complete space-variant polarization analysis and measurement.

Pancharatnam--Berry phase in space-variant polarization-state manipulations with subwavelength gratings.

We report the appearance of a geometrical phase in space-variant polarization-state manipulations. This phase is related to the classic Pancharatnam-Berry phase. We show a method with which to calculate it and experimentally demonstrate its effect, using subwavelength metal stripe space-variant gratings. The experiment is based on a unique grating for converting circularly polarized light at a wavelength of 10.6 mum into an azimuthally polarized beam. Our experimental evidence relies on analysis of far-field images of the resultant polarization.

Spatial Fourier-transform polarimetry using space-variant subwavelength metal-stripe polarizers.

A novel method for rapid polarization measurement is suggested. The method is based on a periodic space-variant polarizer that can be realized by use of subwavelength metal-stripe gratings. The Stokes parameters of the incident beam are determined by Fourier analysis of the space-variant intensity transmitted through the grating, thus permitting real-time polarization measurement. We discuss the design and realization of such polarizers and demonstrate our technique with polarization measurements of CO(2)-laser radiation at a wavelength of 10.6mum.