RESUMEN
Sea turtles are vulnerable to climate change since their reproductive output is influenced by incubating temperatures, with warmer temperatures causing lower hatching success and increased feminization of embryos. Their ability to cope with projected increases in ambient temperatures will depend on their capacity to adapt to shifts in climatic regimes. Here, we assessed the extent to which phenological shifts could mitigate impacts from increases in ambient temperatures (from 1.5 to 3°C in air temperatures and from 1.4 to 2.3°C in sea surface temperatures by 2100 at our sites) on four species of sea turtles, under a "middle of the road" scenario (SSP2-4.5). Sand temperatures at sea turtle nesting sites are projected to increase from 0.58 to 4.17°C by 2100 and expected shifts in nesting of 26-43 days earlier will not be sufficient to maintain current incubation temperatures at 7 (29%) of our sites, hatching success rates at 10 (42%) of our sites, with current trends in hatchling sex ratio being able to be maintained at half of the sites. We also calculated the phenological shifts that would be required (both backward for an earlier shift in nesting and forward for a later shift) to keep up with present-day incubation temperatures, hatching success rates, and sex ratios. The required shifts backward in nesting for incubation temperatures ranged from -20 to -191 days, whereas the required shifts forward ranged from +54 to +180 days. However, for half of the sites, no matter the shift the median incubation temperature will always be warmer than the 75th percentile of current ranges. Given that phenological shifts will not be able to ameliorate predicted changes in temperature, hatching success and sex ratio at most sites, turtles may need to use other adaptive responses and/or there is the need to enhance sea turtle resilience to climate warming.
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Tortugas , Animales , Tortugas/fisiología , Temperatura , Cambio Climático , Reproducción , Razón de MasculinidadRESUMEN
For a Rydberg atom-based sensor to change its sensing frequency, the wavelength of the Rydberg state excitation laser must be altered. The wavelength shifts required can be on the order of 10â nm. A fast-tunable narrow-linewidth laser with broadband tuning capability is required. Here, we present a demonstration of a laser system that can rapidly switch a coupling laser as much as 8â nm in less than 50â µs. The laser system comprises a frequency-stabilized continuous wave laser and an electro-optic frequency comb. A filter enables selection of individual comb lines. A high-speed electro-optic modulator is used to tune the selected comb line to a specific frequency, i.e., an atomic transition. Through Rydberg atom-based sensing experiments, we demonstrate frequency hopping between two Rydberg states and a fast switching time of 400â µs, which we show can be reduced to â¼50â µs with a ping-pong scheme. If updating the RF frequency is not required during frequency hopping, a 200â ns switching time can be achieved. These results showcase the potential of the laser system for advanced Rydberg atom-based radio frequency sensing applications, like communications and radar.
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We demonstrate laser frequency stabilization with at least 6 GHz of offset tunability using an in-phase/quadrature (IQ) modulator to generate electronic sidebands (ESB) on a titanium sapphire laser at 714 nm and we apply this technique to perform isotope shift spectroscopy of 226Ra and 225Ra. By locking the laser to a single resonance of a high finesse optical cavity and adjusting the lock offset, we determine the frequency difference between the magneto-optical trap (MOT) transitions in the two isotopes to be 2630.0 ± 0.3 MHz, a factor of 29 more precise than the previously available data. Using the known value of the hyperfine splitting of the 3P1 level, we calculate the isotope shift for the 1S0 to 3P1 transition to be 2267.0 ± 2.2 MHz, a factor of 8 more precise than the best available value. Our technique could be applied to countless other atomic systems to provide unprecedented precision in isotope shift spectroscopy and other relative frequency comparisons.
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Metallothioneins (MTs) are small, cysteine-rich proteins characterized by a high affinity for monovalent and divalent cations, such as copper and zinc. Of the four known MT isoforms, only, members of the MT 1 and 2 subfamilies are widely expressed, acting as metal chaperones whose primary role is to mediate intracellular zinc homoeostasis. Metallothioneins are potently induced by heavy metals and other sources of oxidative stress where they facilitate metal binding and detoxification as well as free radical scavenging. Metallothionein expression is well documented in the context of viral infection; however, it remains uncertain whether MTs possess specific antiviral roles or whether induction is merely a consequence of cellular stress. To better understand the role of MTs following hepatitis C virus (HCV) infection, we examined MT expression and localization in vitro and in vivo and used a siRNA knockdown approach to ascertain their antiviral efficacy. We confirmed HCV-driven MT induction in vitro and demonstrated MT accumulation in the nucleus of HCV-infected hepatocytes by immunofluorescence. Using a pan-MT siRNA to knock down all members of the MT1 and MT2 subfamilies, we demonstrate that they are mildly antiviral against the JFH1 strain of HCV in vitro (~1.4 fold increase in viral RNA, P < .05). Furthermore, the antiviral effect of zinc treatment against HCV in vitro was mediated through MT induction (P < .05). Our data suggest a potential benefit of using zinc as a low-cost adjunct to current HCV antiviral therapies and suggest that zinc may facilitate the antiviral role of MTs against other viruses.
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Antivirales/metabolismo , Hepatitis C/inmunología , Metalotioneína/metabolismo , Zinc/metabolismo , Antivirales/análisis , Línea Celular , Perfilación de la Expresión Génica , Técnicas de Silenciamiento del Gen , Hepatocitos/química , Hepatocitos/virología , Humanos , Metalotioneína/análisis , Microscopía Fluorescente , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
Multiple Sclerosis (MS) is a neurological condition driven in part by immune cells from the peripheral circulation, the targets for current successful therapies. The autoimmune and MS risk gene ZMIZ1 is underexpressed in blood in people with MS. We show that, from three independent sets of transcriptomic data, expression of ZMIZ1 is tightly correlated with that of hundreds of other genes. Further we show expression is partially heritable (heritability 0.26), relatively stable over time, predominantly in plasmacytoid dendritic cells and non-classical monocytes, and that levels of ZMIZ1 protein expression are reduced in MS. ZMIZ1 gene expression is increased in response to calcipotriol (1,25 Vitamin D3) (p < 0.0003) and associated with Epstein Barr Virus (EBV) EBNA-1 antibody titre (p < 0.004). MS therapies fingolimod and dimethyl fumarate altered blood ZMIZ1 gene expression compared to untreated MS. The phenotype indicates susceptibility to MS, and may correspond with clinical response and represent a novel clinical target.
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Autoinmunidad/genética , Esclerosis Múltiple/etiología , Esclerosis Múltiple/metabolismo , Fenotipo , Factores de Transcripción/genética , Vitamina D/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Biomarcadores , Estudios de Casos y Controles , Células Dendríticas/inmunología , Células Dendríticas/metabolismo , Susceptibilidad a Enfermedades , Infecciones por Virus de Epstein-Barr/complicaciones , Infecciones por Virus de Epstein-Barr/inmunología , Femenino , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Genotipo , Herpesvirus Humano 4/inmunología , Humanos , Patrón de Herencia , Masculino , Persona de Mediana Edad , Monocitos/inmunología , Monocitos/metabolismo , Esclerosis Múltiple/patología , Polimorfismo de Nucleótido Simple , Estaciones del Año , Factores de Transcripción/metabolismo , Vitamina D/farmacología , Adulto JovenRESUMEN
The vitamin D receptor (VDR) is a ligand-activated transcription factor that regulates gene expression in many cell types, including immune cells. It requires binding of 1,25 dihydroxy vitamin D3 (1,25D3) for activation. Many autoimmune diseases show latitude-dependent prevalence and/or association with vitamin D deficiency, and vitamin D supplementation is commonly used in their clinical management. 1,25D3 is regulated by genes associated with the risk of autoimmune diseases and predominantly expressed in myeloid cells. We determined the VDR cistrome in monocytes and monocyte-derived inflammatory (DC1) and tolerogenic dendritic cells (DC2). VDR motifs were highly overrepresented in ChIP-Seq peaks in stimulated monocyte (40%), DC1 (21%) and DC2 (47%), PAsunto(s)
Artritis Reumatoide/genética
, Esclerosis Múltiple/genética
, Receptores de Calcitriol/genética
, Artritis Reumatoide/inmunología
, Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/genética
, Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/metabolismo
, Estudios de Casos y Controles
, Células Dendríticas/metabolismo
, Humanos
, Monocitos/metabolismo
, Esclerosis Múltiple/inmunología
, Polimorfismo Genético
, Receptores de Calcitriol/metabolismo
, Elementos de Respuesta
, Vitamina D/metabolismo
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Single-nucleotide polymorphisms near the interferon lambda 3 (IFNL3) gene predict outcomes to infection and anti-viral treatment in hepatitis C virus (HCV) infection. To identify IFNL3 genotype effects on peripheral blood, we collected phenotype data on 400 patients with genotype 1 chronic hepatitis C (CHC). The IFNL3 responder genotype predicted significantly lower white blood cells (WBCs), as well as lower absolute numbers of monocytes, neutrophils and lymphocytes for both rs8099917 and rs12979860. We sought to define the WBC subsets driving this association using flow cytometry of 67 untreated CHC individuals. Genotype-associated differences were seen in the ratio of CD4CD45RO+ to CD4CD45RO-; CD8CD45RO+ to CD8CD45RO-, NK CD56 dim to bright and monocyte numbers and percentages. Whole blood expression levels of IFNL3, IFNLR1 (interferon lambda receptor 1), IFNLR1-mem (a membrane-associated receptor), IFNLR1-sol (a truncated soluble receptor), MxA and T- and NK (natural killer) cell transcription factors TBX21, GATA3, RORC, FOXP3 and EOMES in two subjects were also determined. CHC patients demonstrated endogenous IFN activation with higher levels of MxA, IFNLR1, IFNLR1-mem and IFNLR1-sol, and IFNL3 genotype-associated differences in transcription factors. Taken together, these data provide evidence of an IFNL3 genotype association with differences in monocyte, T- and NK cell levels in the peripheral blood of patients with CHC. This could underpin genotype associations with spontaneous and treatment-induced HCV clearance and hepatic necroinflammation.
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Hepatitis C Crónica/inmunología , Interleucinas/genética , Antígenos de Diferenciación/metabolismo , Estudios de Cohortes , Citometría de Flujo , Genotipo , Hepacivirus , Humanos , Interferones , Células Asesinas Naturales/citología , Monocitos/citología , Linfocitos T/citología , Factores de Transcripción/metabolismo , Carga ViralRESUMEN
Temperature is arguably the most important abiotic factor influencing the life history of ectotherms. It limits survival and affects all physiological and metabolic processes, including energy and nutrient procurement and processing, development and growth rates, locomotion ability and ultimately reproductive success. However, the influence of temperature on the energetic cost of development has not been thoroughly investigated. We show that in the diamondback moth [Plutella xylostella L. (Lepidoptera: Plutellidae)] rearing temperature (range 10-30°C) affected growth and development rates, the energetic cost of development and fecundity. Rearing at lower temperatures increased development times and slowed growth rate, but resulted in larger adult mass. Fecundity was lowest at 10°C, highest at 15°C and intermediate at temperatures of 20°C and above. At a given rearing temperature fecundity was correlated with pupal mass and most eggs were laid on the first day of oviposition, there was no correlation between total eggs laid and adult longevity. The highest production cost was incurred at 10°C; this decreased with increasing temperature, was minimized in the range 20-25°C, and then increased again at 30°C. These minimized production costs occurred at temperatures close to the intrinsic optimum temperature for this species and may reflect the rearing temperature for optimal fitness. Thus at sub-optimal temperatures greater food resources are required during the development period. Predicted increased temperatures at the margins of the current core distribution of P. xylostella could ameliorate current seasonal effects on fecundity, thereby increasing the probability of winter survival leading to more resilient range expansion and an increased probability of pest outbreaks.
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Mariposas Nocturnas/fisiología , Temperatura , Análisis de Varianza , Animales , Tamaño Corporal , Metabolismo Energético , Fertilidad , Larva/crecimiento & desarrollo , Larva/metabolismo , Larva/fisiología , Longevidad , Mariposas Nocturnas/crecimiento & desarrollo , Mariposas Nocturnas/metabolismo , Consumo de Oxígeno , Pupa/crecimiento & desarrollo , Pupa/metabolismo , Pupa/fisiología , Estaciones del AñoRESUMEN
The ability of two common, site-attached coral-reef fishes to return to their home corals after displacement was investigated in a series of field experiments at One Tree Island, southern Great Barrier Reef. The humbug Dascyllus aruanus was displaced up to 250 m, with 42% of individuals returning home, irrespective of body size, displacement, direction (up or across currents) and route complexity, while for the lemon damselfish Pomacentrus moluccensis 35% of individuals returned overall, with 33% from the greatest displacement, 100 m along a reef edge. Given that the home range of both species is <1 m(2) , over their 10+ year life span, the mechanisms and motivations for such homing ability are unclear but it may allow resilience if fishes are displaced by storm events, allowing rapid return to home corals.
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Arrecifes de Coral , Fenómenos de Retorno al Lugar Habitual , Perciformes , Animales , Antozoos , Peces , QueenslandRESUMEN
The life-history traits of two species of pipefish (Syngnathidae) from seagrass meadows in New South Wales, Australia, were examined to understand whether they enhance resilience to habitat degradation. The spotted pipefish Stigmatopora argus and wide-bodied pipefish Stigmatopora nigra exhibit some of the shortest life spans known for vertebrates (longevity up to 150 days) and rapid maturity (male S. argus 35 days after hatching (DAH) and male S. nigra at 16-19 DAH), key characteristics of opportunistic species. Growth rates of both species were extremely rapid (up to 2 mm day(-1) ), with seasonal and sex differences in growth rate. It is argued that short life spans and high growth rates may be advantageous for these species, which inhabit one of the most threatened marine ecosystems on earth.
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Ecosistema , Smegmamorpha/crecimiento & desarrollo , Animales , Australia , Femenino , Longevidad , Masculino , Nueva Gales del Sur , Maduración SexualRESUMEN
Increasing human population and intensive land use combined with a warming climate and chronically diminished snowpacks are putting more strain on water resources in the western United States. Properly functioning riparian systems slow runoff and store water, thus regulating extreme flows; however, riparian areas across the west are in a degraded condition with a majority of riparian systems not in proper functioning condition, and with widespread catastrophic erosion of water-storing peat and organic soils. Headcuts are the leading edge of catastrophic channel erosion. We used aerial imagery (1.4-3.3-cm pixel) to locate 163 headcuts in riparian areas in the Sweetwater subbasin of central Wyoming. We found 1-m-the generally available standard resolution for land management-and 30-cm pixel imagery to be inadequate for headcut identification. We also used Structure-from-Motion models built from ground-acquired imagery to model 18 headcuts from which we measured soil loss of 425-720 m3. Normalized by channel length, this represents a loss of 1.1-1.8 m3 m(-1) channel. Monitoring headcuts, either from ground or aerial imagery, provides an objective indicator of sustainable riparian land management and identifies priority disturbance-mitigation areas. Image-based headcut monitoring must use data on the order of 3.3 cm ground sample distance, or greater resolution, to effectively capture the information needed for accurate assessments of riparian conditions.
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Conservación de los Recursos Naturales , Monitoreo del Ambiente/métodos , Sistemas de Información Geográfica , Geografía , Suelo , Humedales , WyomingRESUMEN
OBJECTIVE: The natural course of chronic hepatitis C varies widely. To improve the profiling of patients at risk of developing advanced liver disease, we assessed the relative contribution of factors for liver fibrosis progression in hepatitis C. DESIGN: We analysed 1461 patients with chronic hepatitis C with an estimated date of infection and at least one liver biopsy. Risk factors for accelerated fibrosis progression rate (FPR), defined as ≥ 0.13 Metavir fibrosis units per year, were identified by logistic regression. Examined factors included age at infection, sex, route of infection, HCV genotype, body mass index (BMI), significant alcohol drinking (≥ 20 g/day for ≥ 5 years), HIV coinfection and diabetes. In a subgroup of 575 patients, we assessed the impact of single nucleotide polymorphisms previously associated with fibrosis progression in genome-wide association studies. Results were expressed as attributable fraction (AF) of risk for accelerated FPR. RESULTS: Age at infection (AF 28.7%), sex (AF 8.2%), route of infection (AF 16.5%) and HCV genotype (AF 7.9%) contributed to accelerated FPR in the Swiss Hepatitis C Cohort Study, whereas significant alcohol drinking, anti-HIV, diabetes and BMI did not. In genotyped patients, variants at rs9380516 (TULP1), rs738409 (PNPLA3), rs4374383 (MERTK) (AF 19.2%) and rs910049 (major histocompatibility complex region) significantly added to the risk of accelerated FPR. Results were replicated in three additional independent cohorts, and a meta-analysis confirmed the role of age at infection, sex, route of infection, HCV genotype, rs738409, rs4374383 and rs910049 in accelerating FPR. CONCLUSIONS: Most factors accelerating liver fibrosis progression in chronic hepatitis C are unmodifiable.
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Hepacivirus/genética , Hepatitis C Crónica/complicaciones , Cirrosis Hepática/etiología , Polimorfismo de Nucleótido Simple , ARN Viral/análisis , Medición de Riesgo/métodos , Biopsia , Progresión de la Enfermedad , Femenino , Estudio de Asociación del Genoma Completo , Hepatitis C Crónica/virología , Humanos , Incidencia , Cirrosis Hepática/diagnóstico , Cirrosis Hepática/epidemiología , Masculino , Estudios Retrospectivos , Factores de Riesgo , Suiza/epidemiología , Factores de TiempoRESUMEN
The observation of strongly interacting many-body phenomena in atomic gases typically requires ultracold samples. Here we show that the strong interaction potentials between Rydberg atoms enable the observation of many-body effects in an atomic vapor, even at room temperature. We excite Rydberg atoms in cesium vapor and observe in real time an out-of-equilibrium excitation dynamics that is consistent with an aggregation mechanism. The experimental observations show qualitative and quantitative agreement with a microscopic theoretical model. Numerical simulations reveal that the strongly correlated growth of the emerging aggregates is reminiscent of soft-matter type systems.
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The IFNL3 genotype predicts the clearance of hepatitis C virus (HCV), spontaneously and with interferon (IFN)-based therapy. The responder genotype is associated with lower expression of interferon stimulated genes (ISGs) in liver biopsies from chronic hepatitis C patients. However, ISGs represent many interacting molecular pathways, and we hypothesised that the IFNL3 genotype may produce a characteristic pattern of ISG expression explaining the effect of genotype on viral clearance. For the first time, we identified an association between a cluster of ISGs, the metallothioneins (MTs) and IFNL3 genotype. Importantly, MTs were significantly upregulated (in contrast to most other ISGs) in HCV-infected liver biopsies of rs8099917 responders. An association between lower fibrosis scores and higher MT levels was demonstrated underlying clinical relevance of this association. As expected, overall ISGs were significantly downregulated in biopsies from subjects with the IFNL3 rs8099917 responder genotype (P=2.38 × 10(-7)). Peripheral blood analysis revealed paradoxical and not previously described findings with upregulation of ISGs seen in the responder genotype (P=1.00 × 10(-4)). The higher MT expression in responders may contribute to their improved viral clearance and MT-inducing agents may be useful adjuncts to therapy for HCV. Upregulation of immune cell ISGs in responders may also contribute to the IFNL3 genotype effect.
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Hepatitis C Crónica/tratamiento farmacológico , Interleucinas/genética , Metalotioneína/biosíntesis , Carga Viral/genética , Genotipo , Hepacivirus , Humanos , Factores Reguladores del Interferón/genética , Interferón-alfa/uso terapéutico , Interferones , Hígado/patología , Hígado/virología , Cirrosis Hepática/genética , Polietilenglicoles/uso terapéutico , Polimorfismo de Nucleótido Simple , Proteínas Recombinantes/uso terapéutico , Ribavirina/uso terapéutico , Resultado del Tratamiento , Regulación hacia ArribaRESUMEN
The effect of water temperature on growth responses of three common seagrass fish species that co-occur as juveniles in the estuaries in Sydney (34° S) but have differing latitudinal ranges was measured: Pelates sexlineatus (subtropical to warm temperate: 27-35°S), Centropogon australis (primarily subtropical to warm temperate: 24-37°S) and Acanthaluteres spilomelanurus (warm to cool temperate: below 32°S). Replicate individuals of each species were acclimated over a 7 day period in one of three temperature treatments (control: 22°C, low: 18°C and high: 26°C) and their somatic growth was assessed within treatments over 10 days. Growth of all three species was affected by water temperature, with the highest growth of both northern species (P. sexlineatus and C. australis) at 22 and 26°C, whereas growth of the southern ranging species (A. spilomelanurus) was reduced at temperatures higher than 18°C, suggesting that predicted increase in estuarine water temperatures through climate change may change relative performance of seagrass fish assemblages.
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Cambio Climático , Ecosistema , Perciformes/crecimiento & desarrollo , Temperatura , Animales , Australia , EstuariosRESUMEN
This study provides the first assessment of fish associations with oil and gas structures located in deep water (85-175 m) on Australia's north-west continental shelf, using rare oil industry video footage obtained from remotely operated vehicles. A diverse range of taxa were observed associating with the structures, including reef-dependent species and transient pelagic species. Ten commercially fished species were observed, the most abundant of which was Lutjanus argentimaculatus, with an estimated biomass for the two deepest structures (Goodwyn and Echo) of 109 kg.
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Biota , Industria Procesadora y de Extracción , Peces , Animales , Australia , Biomasa , Industria Procesadora y de Extracción/instrumentación , Peces/clasificación , Dinámica Poblacional , Grabación en VideoRESUMEN
IL28B polymorphisms strongly predict spontaneous and treatment-induced clearance of hepatitis C virus (HCV) infection. A recent study proposed a 32-base pair deletion in the CC-chemokine receptor 5 (CCR5) gene (CCR5-Δ32) interacting with the IL28B polymorphisms to influence spontaneous HCV clearance. The aim of this study was to clarify the role of CCR5-Δ32 in treatment-induced clearance of chronic hepatitis C (CHC). A cross-sectional cohort of 813 Caucasian patients with CHC genotype 1 (365 responders and 448 non-responders) who had received standard of care dual therapy with interferon (IFN)-α and ribavirin (RBV) was genotyped for the CCR5-Δ32 and IL28B polymorphisms to examine their interaction with respect to treatment response. CCR5-Δ32 did not influence treatment-induced recovery to IFN-α/RBV in CHC, and did not improve prediction of sustained virological response in the context of the IL28B polymorphisms in a multivariate model. CCR5-Δ32 homozygotes were significantly more frequent in those with CHC than healthy controls in the European cohorts (2.9% vs 0.4%, P<0.0001), but not in Australians of European ancestry. In conclusion, CCR5-Δ32 does not influence treatment response in the context of IL28B polymorphisms. Although CCR5-Δ32 may affect viral clearance within closely controlled geographical and genetic environments, we found no effect in larger cohorts treated with dual therapy.
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Antivirales/uso terapéutico , Hepatitis C Crónica/tratamiento farmacológico , Interleucinas/genética , Polimorfismo de Nucleótido Simple , Receptores CCR5/genética , Eliminación de Secuencia , Adulto , Australia , Secuencia de Bases , Estudios de Cohortes , Estudios Transversales , Quimioterapia Combinada , Epistasis Genética , Europa (Continente) , Femenino , Genotipo , Hepatitis C Crónica/etnología , Hepatitis C Crónica/genética , Humanos , Interferón-alfa/uso terapéutico , Interferones , Masculino , Persona de Mediana Edad , Análisis Multivariante , Pronóstico , Ribavirina/uso terapéutico , Resultado del Tratamiento , Población Blanca/genéticaRESUMEN
We previously found an association between faster CD4+ T-cell recovery in HIV-infected patients receiving combination antiretroviral therapy (cART) and interleukin-7 receptor-α (IL-7Rα) haplotype-2 in a predominantly Caucasian cohort. This study aims to determine whether this association was also significant in Africans. Patients were recruited from the Uganda AIDS Rural Treatment Outcomes (UARTO) cohort (n=352). We used survival analysis and linear mixed modelling (LMM) to determine factors associated with CD4 T-cell recovery. Eight IL-7Rα single-nucleotide polymorphisms (SNPs) were genotyped in both Africans and Caucasians (n=57). Soluble (s)IL-7Rα levels were measured by ELISA. In UARTO, IL-7Rα haplotype-2 was associated with slower CD4 T-cell recovery following cART by using survival analysis (P=0.020) and no association was found with LMM (P=0.958). The tagging-SNP for IL-7Rα haplotype-2 (rs6897932) was associated with decreased sIL-7Rα (P<0.001). The haplotypes for the IL-7Rα were significantly different in Africans and Caucasians. Using IL-7Rα genotypes we found slower CD4 T-cell recovery in UARTO patients was still associated with rs6897932 (P=0.009) and rs3194051 was associated with faster CD4 T-cell recovery (P=0.006). Unlike Caucasians, we did not demonstrate a significant association between IL-7Rα haplotype 2 and faster CD4 T-cell recovery in Africans. The IL-7Rα SNPs associated with CD4 T-cell recovery following cART differ in African and Caucasian cohorts.
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Linfocitos T CD4-Positivos/inmunología , Infecciones por VIH/genética , Infecciones por VIH/inmunología , Polimorfismo de Nucleótido Simple , Receptores de Interleucina-7/genética , Adulto , Terapia Antirretroviral Altamente Activa , Población Negra/genética , Recuento de Linfocito CD4 , Linfocitos T CD4-Positivos/metabolismo , Femenino , Frecuencia de los Genes , Estudios de Asociación Genética , Infecciones por VIH/tratamiento farmacológico , Haplotipos , Humanos , Masculino , Pronóstico , Receptores de Interleucina-7/sangre , Análisis de Supervivencia , Población Blanca/genéticaRESUMEN
We observe ultralong-range blueshifted Cs(2) molecular states near ns(1/2) Rydberg states in an optical dipole trap, where 31≤n≤34. The accidental near degeneracy of (n-4)l and ns Rydberg states for l>2 in Cs, due to the small fractional ns quantum defect, leads to nonadiabatic coupling among these states, producing potential wells above the ns thresholds. Two important consequences of admixing high angular momentum states with ns states are the formation of large permanent dipole moments, ~15-100 Debye, and accessibility of these states via two-photon association. The observed states are in excellent agreement with theory.
RESUMEN
A mathematical model based on conservation of energy has been developed and used to simulate the temperature responses of cones of the Australian cycads Macrozamia lucida and Macrozamia. macleayi during their daily thermogenic cycle. These cones generate diel midday thermogenic temperature increases as large as 12 °C above ambient during their approximately two week pollination period. The cone temperature response model is shown to accurately predict the cones' temperatures over multiple days as based on simulations of experimental results from 28 thermogenic events from 3 different cones, each simulated for either 9 or 10 sequential days. The verified model is then used as the foundation of a new, parameter estimation based technique (termed inverse calorimetry) that estimates the cones' daily metabolic heating rates from temperature measurements alone. The inverse calorimetry technique's predictions of the major features of the cones' thermogenic metabolism compare favorably with the estimates from conventional respirometry (indirect calorimetry). Because the new technique uses only temperature measurements, and does not require measurements of oxygen consumption, it provides a simple, inexpensive and portable complement to conventional respirometry for estimating metabolic heating rates. It thus provides an additional tool to facilitate field and laboratory investigations of the bio-physics of thermogenic plants.