RESUMEN
In our animal experiments the hypothesis was tested that a high-fiber (HF) diet reduces tumor promotion by interruption of the enterohepatic circulation resulting in lowered estrogen exposure of the estrogen-sensitive tissue. In the first experiment the development of N-nitrosomethylurea (NMU) induced mammary tumors was investigated. One group of rats (HF) was fed a HF diet (11% fiber, based on wheat bran), the other group (LF) fed a low-fiber diet (0.5% fiber, based on white wheat flour). Tumor incidence (90 and 80%, respectively) and latency (121 and 128 days, respectively) were similar in the HF and LF groups. Compared to the LF group, HF rats had lower tumor weights (0.16 vs 0.55 g; P less than 0.01) and a slightly lower tumor multiplicity (1.8 vs 2.8 tumors per tumor-bearing rat). These differences were reduced after adjustment for body weight. In a second experiment rats, not treated with the carcinogen, were kept on the same HF and LF diets. From these rats 24-h urine and feces and orbital blood samples were collected for analysis of (un)conjugated estrogens. The excretion of both free and conjugated estrogens in fecal samples was about 3-fold higher in HF rats than in LF rats. During the basal period of the cycle urinary excretion of estrone was lower in HF rats (mean 9.7 ng/day) than in LF rats (mean 13.0 ng/day; P less than 0.05). It is concluded that wheat bran interrupts the enterohepatic circulation of estrogens, but plasma levels are not affected. Whether the development of mammary tumors is reduced by the introduction of specific components of wheat bran, or by a reduced body weight due to a lower (effective) energy intake remains to be determined.
Asunto(s)
Fibras de la Dieta/farmacología , Estrógenos/sangre , Neoplasias Mamarias Experimentales/prevención & control , Animales , Estrógenos/orina , Heces/enzimología , Femenino , Glucuronidasa/metabolismo , Neoplasias Mamarias Experimentales/sangre , Neoplasias Mamarias Experimentales/patología , Metilnitrosourea , Ratas , Ratas Endogámicas F344 , TriticumRESUMEN
We have studied the effects of short-term exercise on the degradation rate of estradiol (E2) measured as the metabolic clearance rate (MCRE2). Six young women (mean age 20.7 yr) volunteered for this study in which we investigated the influence of a submaximal bicycle ergometer load on the MCRE2. All measurements were done in the morning of the 7th to 10th day of the menstrual cycle. [3H]estradiol 17 beta ([3H]E2) was administered intravenously at a constant rate by an infusion pump. During the exercise period on the bicycle ergometer (70% VO2max, 10 min) and the recovery period (25% VO2max, 30 min), several blood samples were taken in which the [3H]E2 concentration was estimated. The results showed a strong decrease in the MCRE2 (range 18-67%) at the end of the work load for all the volunteers. At the end of the recovery period, the MCR was still lower than the basal value (range 30-50%). The possible mechanisms and relevance of these exercise-induced MCR changes of estradiol are discussed.
Asunto(s)
Estradiol/metabolismo , Esfuerzo Físico , Adulto , Femenino , Humanos , MenstruaciónRESUMEN
Dietary factors are now considered to be among the most important environmental risk determinants for cancer. In addition to epidemiological studies, experimental animal studies are an important tool to investigate dietary modulation in carcinogenesis. Results of recent experimental studies on the effect of some nutrients indicate that vitamin A did show an inverse relation with the occurrence of preneoplastic respiratory lesions but not with respiratory tract tumors in benzo[a]pyrene-induced respiratory carcinogenesis. Dietary fat increases respiratory tract tumors and preneoplastic lesions. In colon carcinogenesis, a fat-fiber interrelation was noticed in 1,2-dimethyl-hydrazine- and N-methyl-N'-nitro-N-nitrosoguanidine-induced tumors. Preliminary results in prostate carcinogenesis indicate that dietary fat did not influence the incidence of prostate cancer in a recently developed rat model. Some possible mechanisms in colon and prostate carcinogenesis are discussed.
Asunto(s)
Neoplasias del Colon/etiología , Dieta , Modelos Animales de Enfermedad , Neoplasias Pulmonares/etiología , Neoplasias de la Próstata/etiología , 1,2-Dimetilhidrazina , Andrógenos/sangre , Animales , Benzo(a)pireno , Neoplasias del Colon/inducido químicamente , Grasas de la Dieta/efectos adversos , Fibras de la Dieta/farmacología , Proteínas en la Dieta/farmacología , Dimetilhidrazinas , Sinergismo Farmacológico , Métodos Epidemiológicos , Humanos , Neoplasias Pulmonares/inducido químicamente , Masculino , Metilnitronitrosoguanidina , Modelos Biológicos , Neoplasias de la Próstata/inducido químicamente , Vitamina A/farmacologíaRESUMEN
The influence of amount and type of dietary fat on circulating concentrations of prolactin and estradiol-17 beta in female F344 rats from which blood was sampled by decapitation under ether anesthesia was compared with that in rats from which blood was collected without anesthesia. The animals were fed isonutrient (adjusted for differences in energy density) semipurified diets containing 5% or 20% (by weight) sunflower seed oil or lard. Blood was sampled by decapitation with or without standardized ether anesthesia during the afternoon of proestrus-estrus or the morning of metestrus-diestrus, as determined by examination of vaginal smears. Plasma hormone concentrations were measured by radioimmunoassay. Prolactin levels were lower during proestrus-estrus in rats fed a low-fat diet than in animals fed a high-fat diet, statistically independent of the type of dietary fat, but only when blood was sampled by decapitation under ether anesthesia [p = 0.0384, 2-way analysis of variance (ANOVA)]. No such difference was found in rats decapitated without anesthesia. This effect of amount of dietary fat on prolactin in proestrus-estrus animals anesthetized with ether was predominantly present in animals fed polyunsaturated fat (p < 0.05, 1-way ANOVA and Tukey's test) and was statistically not significant in rats fed saturated fat diets. During metestrus-diestrus, prolactin levels were significantly lower in animals fed a high-saturated fat diet than in those fed low-saturated fat, low-unsaturated fat, or high-unsaturated fat diets, independent of the blood sampling conditions (p < 0.05, 2-way ANOVA and Tukey's test). No consistent effects on estradiol-17 beta levels were found in type or amount of dietary fat or in presence or absence of ether anesthesia before decapitation. Growth, apparent digestibility of fat, and caloric intake were similar in all four dietary groups, but food consumption was higher and food conversion efficiency was lower in animals fed low-fat diets than in those fed high-fat diets. This study confirms the hypothesis that effects of dietary fat, particularly polyunsaturated fat, on circulating prolactin occur only during (ether) stress. Because stress is a frequent and normal phenomenon, this observation implies that the mammary glands of animals with a high dietary intake of polyunsaturated fat are frequently exposed to higher circulating prolactin concentrations than rats fed a low-fat diet, which may be a major mechanism by which dietary fat enhances rat mammary carcinogenesis.