Prognostic factors for deterioration of quality of life one year after admission to ICU for severe SARS-COV2 infection.

PURPOSE: Patients with COVID-19 present long-term symptoms collectively known as "post-COVID syndrome". Long-term manifestations are more frequent in patients requiring admission to Intensive Care Units (ICU), but the risk factors for their development are still unknown. This study explores the quality of life of patients with severe COVID-19 one year after their discharge from ICU. METHODS: Prospective observational study including 106 patients admitted to the ICU of a tertiary care hospital between March and August 2020. We analysed quality of life using the EuroQol-5D index (EQ-5D). We evaluated as possible risk factors associated with a worse value of the EQ-5D index the medical record, the clinical situation at hospital admission, the clinical situation at ICU admission and evolution in the ICU. As a secondary objective, we explored the presence of other frequent symptoms. RESULTS: Most patients (55.4%) reported that their quality of life worsened one year after admission. The mean perceived health status, on the EQ-VAS scale (0-100), was 70.4, with a median of 70 (RI 60-90). The median EQ-5D index was 0.91 (RI 0.76-1). The factors independently related to lower quality of life were female sex and duration of mechanical ventilation. Almost all (91%) of the patients had sequelae one year after discharge from the ICU. The most frequent manifestations were neuropsychiatric (71%). CONCLUSIONS: Critically ill COVID-19 patients worsen their quality of life more than one year after discharge. Female sex and duration of mechanical ventilation predict a lower quality of life assessed by the EQ-5D index.

Isolation of viable porcine islets by selective osmotic shock without enzymatic digestion.

Islet transplantation is a potential cure for type 1 diabetes, but clinical results have been disappointing. Currently, islet isolation is by enzymatic digestion of the pancreas which has significant pitfalls: warm ischemia exposure, collagenase-induced damage to the islet mass and viability, poor reproducibility, high cost, a relatively low number of islets obtained per whole pancreas, and selection of islets for collagenase resistance rather than for glucose responsiveness. In the present study we performed a series of experiments in a porcine model to demonstrate the feasibility of a new isolation method based on selective osmotic shock (SOS) using very high glucose solutions, doubling or tripling physiological osmotic strength. The SOS method can be carried out at room temperature or in the cold eliminating warm ischemia time which damages the islets. The SOS method does not depend on the texture of the pancreas so all pancreases can be processed identically and the process can be fully automated. The SOS method isolates all the islets of the pancreas regardless of size and shape allowing a greater number of islets to be harvested. The SOS method avoids exposure to toxins in collagenase solutions, is inexpensive and selects for islets with high concentrations of Glut 2 transporters, representing the best glucose responding islets. The SOS method showed a comparable recovery of islets from young pig pancreas and the islets showed improved viability. We conclude that the selective osmotic shock (SOS) method of separating islets from the pancreatic tissue is superior to the collagenase method.

Organ donation in an aging population: the experience of the last 8 years in Galicia.

BACKGROUND: Mean organ donor age has increased in recent years, conditioned by causes of death and population profile. We analyzed organ donation in Galicia during the last 8 years. MATERIALS AND METHODS: We analyzed donors in Galicia between 2000 and 2007, studying age, cause of death, donation effectiveness, and organs transplanted per donor. RESULTS: Donation rates fluctuated between 29 and 35 donors per million population (pmp) during the study period. Mean donor age increased from 51.6 years (Spain, 47.1 years) in 2000 to 63.3 years (Spain, 53.4 years) in 2007. Donors of 75 years or older increased from 7.5% to 27.2%. Cerebrovascular accident (CVA) as cause of death rose from 57% in 2000 to 75.3% in 2007. The variations in the proportions of organs transplanted between 2000 and 2007 were: kidney, 68.8% to 53.7%; liver, 86% to 88.9%; heart, 33.5% to 9.9%; and lung, 10.8% to 9.9%. Valid transplanted organs per donor decreased from 2.8 to 2.2, and effective donors from 93.5% (Spain, 90.3%) to 92.6% (Spain, 87.8%) with a mean of 94%. CONCLUSIONS: The population in Galicia has aged considerably and is widely dispersed. Despite the increased mean donor age, the percentage of effective donors has not diminished and donor rates have remained stable. This reflects the extremely conscientious attitude of transplant coordinators and the high degree of activity of transplant teams in Galicia.