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J Cell Sci ; 129(13): 2493-9, 2016 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-27199373

RESUMEN

Mouse ovarian germ cells enter meiosis in a wave that propagates from anterior to posterior, but little is known about contribution of germ cells to initiation or propagation of meiosis. In a Ror2 mutant with diminished germ cell number and migration, we find that overall timing of meiotic initiation is delayed at the population level. We use chemotherapeutic depletion to exclude a profoundly reduced number of germ cells as a cause for meiotic delay. We rule out sex reversal or failure to specify somatic support cells as contributors to the meiotic phenotype. Instead, we find that anomalies in the distribution of germ cells as well as gonad shape in mutants contribute to aberrant initiation of meiosis. Our analysis supports a model of meiotic initiation via diffusible signal(s), excludes a role for germ cells in commencing the meiotic wave and furnishes the first phenotypic demonstration of the wave of meiotic entry. Finally, our studies underscore the importance of considering germ cell migration defects while studying meiosis to discern secondary effects resulting from positioning versus primary meiotic entry phenotypes.


Asunto(s)
Células Germinativas/metabolismo , Gónadas/patología , Meiosis/genética , Receptores Huérfanos Similares al Receptor Tirosina Quinasa/genética , Animales , Recuento de Células , Movimiento Celular/genética , Forma de la Célula/genética , Femenino , Células Germinativas/crecimiento & desarrollo , Células Germinativas/patología , Gónadas/crecimiento & desarrollo , Ratones , Mutación , Ovario/crecimiento & desarrollo , Ovario/metabolismo , Ovario/patología , Transducción de Señal/genética
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