RESUMEN
Our aim is to study selected cerebrospinal fluid (CSF) glycerophospholipids (GP) that are important in brain pathophysiology. We recruited cognitively healthy (CH), minimally cognitively impaired (MCI), and late onset Alzheimer's disease (LOAD) study participants and collected their CSF. After fractionation into nanometer particles (NP) and supernatant fluids (SF), we studied the lipid composition of these compartments. LC-MS/MS studies reveal that both CSF fractions from CH subjects have N-acyl phosphatidylethanolamine, 1-radyl-2-acyl-sn-glycerophosphoethanolamine (PE), 1-radyl-2-acyl-sn-glycerophosphocholine (PC), 1,2-diacyl-sn-glycerophosphoserine (PS), platelet-activating factor-like lipids, and lysophosphatidylcholine (LPC). In the NP fraction, GPs are enriched with a mixture of saturated, monounsaturated, and polyunsaturated fatty acid species, while PE and PS in the SF fractions are enriched with PUFA-containing molecular species. PC, PE, and PS levels in CSF fractions decrease progressively in participants from CH to MCI, and then to LOAD. Whereas most PC species decrease equally in LOAD, plasmalogen species account for most of the decrease in PE. A significant increase in the LPC-to-PC ratio and PLA2 activity accompanies the GP decrease in LOAD. These studies reveal that CSF supernatant fluid and nanometer particles have different GP composition, and that PLA2 activity accounts for altered GPs in these fractions as neurodegeneration progresses.
Asunto(s)
Enfermedad de Alzheimer/líquido cefalorraquídeo , Glicerofosfolípidos/líquido cefalorraquídeo , Fosfolipasas A2/líquido cefalorraquídeo , Anciano , Anciano de 80 o más Años , Enfermedad de Alzheimer/enzimología , Enfermedad de Alzheimer/patología , Femenino , Glicerofosfolípidos/aislamiento & purificación , Humanos , Metabolismo de los Lípidos , Masculino , Espectrometría de Masa por Ionización de Electrospray , Espectrometría de Masas en TándemRESUMEN
BACKGROUND: Type 2 diabetes mellitus may affect profoundly the quality of life of patients. AIM: To assess health related quality of life among patients with Type 2 Diabetes Mellitus. MATERIAL AND METHODS: The Diabetes Quality of Life (DQOL) questionnaire was applied to 296 patients with diabetes mellitus aged 63 ± lO years (201 women) seen in primary health care centers. RESULTS: The concern about the future effects of diabetes was the worst evaluated domain. Women perceived a lower health related quality of life than men. There was an inverse correlation between age and satisfaction with treatment, concern about vocational, social and future effects of the disease. CONCLUSIONS: Type 2 diabetes affects health related quality of life, especially in some specific domains such as perception of the future.
Asunto(s)
Diabetes Mellitus Tipo 2/psicología , Estado de Salud , Calidad de Vida/psicología , Adulto , Anciano , Anciano de 80 o más Años , Complicaciones de la Diabetes/psicología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Satisfacción del Paciente , Autoinforme , Factores Socioeconómicos , Encuestas y Cuestionarios , Adulto JovenRESUMEN
Background: Type 2 diabetes mellitus may affect profoundly the quality oflife of patients. Aim: To assess health related quality oflife among patients with Type 2 Diabetes Mellitus. Material ana Methods: The Diabetes Quality ofLife (DQOL) questionnaire was applied to 296patients with diabetes mellitus aged 63 ± lOyears (201 women) seen in primary health care centers. Results: The concern about the future effects of diabetes was the worst evaluated domain. Women perceived a lower health related quality oflife than men. There was an inverse correlation between age and satisfaction with treatment, concern about vocational, social and future effects of the disease. Conclusions: Type 2 diabetes affects health related quality of life, especially in some specific domains such as perception of the future.