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1.
Genome Res ; 26(1): 130-9, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26560630

RESUMEN

We have generated an improved assembly and gene annotation of the pig X Chromosome, and a first draft assembly of the pig Y Chromosome, by sequencing BAC and fosmid clones from Duroc animals and incorporating information from optical mapping and fiber-FISH. The X Chromosome carries 1033 annotated genes, 690 of which are protein coding. Gene order closely matches that found in primates (including humans) and carnivores (including cats and dogs), which is inferred to be ancestral. Nevertheless, several protein-coding genes present on the human X Chromosome were absent from the pig, and 38 pig-specific X-chromosomal genes were annotated, 22 of which were olfactory receptors. The pig Y-specific Chromosome sequence generated here comprises 30 megabases (Mb). A 15-Mb subset of this sequence was assembled, revealing two clusters of male-specific low copy number genes, separated by an ampliconic region including the HSFY gene family, which together make up most of the short arm. Both clusters contain palindromes with high sequence identity, presumably maintained by gene conversion. Many of the ancestral X-related genes previously reported in at least one mammalian Y Chromosome are represented either as active genes or partial sequences. This sequencing project has allowed us to identify genes--both single copy and amplified--on the pig Y Chromosome, to compare the pig X and Y Chromosomes for homologous sequences, and thereby to reveal mechanisms underlying pig X and Y Chromosome evolution.


Asunto(s)
Cromosomas de los Mamíferos/genética , Evolución Molecular , Porcinos/genética , Cromosoma X/genética , Cromosoma Y/genética , Animales , Secuencia de Bases , Gatos/genética , Perros/genética , Femenino , Conversión Génica , Expresión Génica , Biblioteca de Genes , Orden Génico , Humanos , Masculino , Datos de Secuencia Molecular , Alineación de Secuencia , Análisis de Secuencia de ADN
2.
J Clin Microbiol ; 57(6)2019 06.
Artículo en Inglés | MEDLINE | ID: mdl-30894439

RESUMEN

There is growing evidence for the value of bacterial whole-genome sequencing in hospital outbreak investigations. Our aim was to develop methods that support efficient and accurate low-throughput clinical sequencing of methicillin-resistant Staphylococcus aureus (MRSA) isolates. Using a test panel of 25 MRSA isolates previously associated with outbreak investigations, we devised modifications to library preparation that reduced the processing time by 1 hour. We determined the maximum number of isolates that could be sequenced per run using an Illumina MiniSeq platform and a 13-hour (overnight) run time, which equated to 21 MRSA isolates and 3 controls (no template, positive, and negative). Repeatability and reproducibility assays based on this sequencing methodology demonstrated 100% accuracy in assigning species and sequence type (ST) and in detecting mecA Established genetic relatedness between isolates was recapitulated. Quality control (QC) metrics were evaluated over nine sequencing runs. Of the test panel MRSA genomes, 168/173 (97%) passed QC metrics based on the correct species assigned, detection of mecA and ST, and depth/coverage metrics. An evaluation of contamination in these 9 runs showed that positive and negative controls and test MRSA sequence files contained <0.14% and <0.48% of fragments that matched another species, respectively. Deliberate contamination experiments confirmed that this was insufficient to impact data interpretation. These methods support reliable and reproducible clinical MRSA sequencing with a turnaround time (from DNA extraction to availability of data files) of 24 hours.


Asunto(s)
Genoma Bacteriano , Staphylococcus aureus Resistente a Meticilina/genética , Infecciones Estafilocócicas/diagnóstico , Infecciones Estafilocócicas/microbiología , Secuenciación Completa del Genoma , Pruebas Diagnósticas de Rutina , Humanos , Laboratorios de Hospital , Staphylococcus aureus Resistente a Meticilina/aislamiento & purificación , Técnicas Microbiológicas , Tipificación de Secuencias Multilocus , Secuenciación Completa del Genoma/métodos
3.
Nature ; 496(7446): 498-503, 2013 Apr 25.
Artículo en Inglés | MEDLINE | ID: mdl-23594743

RESUMEN

Zebrafish have become a popular organism for the study of vertebrate gene function. The virtually transparent embryos of this species, and the ability to accelerate genetic studies by gene knockdown or overexpression, have led to the widespread use of zebrafish in the detailed investigation of vertebrate gene function and increasingly, the study of human genetic disease. However, for effective modelling of human genetic disease it is important to understand the extent to which zebrafish genes and gene structures are related to orthologous human genes. To examine this, we generated a high-quality sequence assembly of the zebrafish genome, made up of an overlapping set of completely sequenced large-insert clones that were ordered and oriented using a high-resolution high-density meiotic map. Detailed automatic and manual annotation provides evidence of more than 26,000 protein-coding genes, the largest gene set of any vertebrate so far sequenced. Comparison to the human reference genome shows that approximately 70% of human genes have at least one obvious zebrafish orthologue. In addition, the high quality of this genome assembly provides a clearer understanding of key genomic features such as a unique repeat content, a scarcity of pseudogenes, an enrichment of zebrafish-specific genes on chromosome 4 and chromosomal regions that influence sex determination.


Asunto(s)
Secuencia Conservada/genética , Genoma/genética , Pez Cebra/genética , Animales , Cromosomas/genética , Evolución Molecular , Femenino , Genes/genética , Genoma Humano/genética , Genómica , Humanos , Masculino , Meiosis/genética , Anotación de Secuencia Molecular , Seudogenes/genética , Estándares de Referencia , Procesos de Determinación del Sexo/genética , Proteínas de Pez Cebra/genética
4.
BMC Genomics ; 16: 442, 2015 Jun 09.
Artículo en Inglés | MEDLINE | ID: mdl-26055083

RESUMEN

BACKGROUND: Amplified gene families on sex chromosomes can harbour genes with important biological functions, especially relating to fertility. The Y-linked heat shock transcription factor (HSFY) family has become amplified on the Y chromosome of the domestic pig (Sus scrofa), in an apparently independent event to an HSFY expansion on the Y chromosome of cattle (Bos taurus). Although the biological functions of HSFY genes are poorly understood, they appear to be involved in gametogenesis in a number of mammalian species, and, in cattle, HSFY gene copy number may correlate with levels of fertility. RESULTS: We have investigated the HSFY family in domestic pig, and other suid species including warthog, bushpig, babirusa and peccaries. The domestic pig contains at least two amplified variants of HSFY, distinguished predominantly by presence or absence of a SINE within the intron. Both these variants are expressed in testis, and both are present in approximately 50 copies each in a single cluster on the short arm of the Y. The longer form has multiple nonsense mutations rendering it likely non-functional, but many of the shorter forms still have coding potential. Other suid species also have these two variants of HSFY, and estimates of copy number suggest the HSFY family may have amplified independently twice during suid evolution. CONCLUSIONS: The HSFY genes have become amplified in multiple species lineages independently. HSFY is predominantly expressed in testis in domestic pig, a pattern conserved with cattle, in which HSFY may play a role in fertility. Further investigation of the potential associations of HSFY with fertility and testis development may be of agricultural interest.


Asunto(s)
Expansión de las Repeticiones de ADN , Porcinos/genética , Factores de Transcripción/genética , Cromosoma Y/genética , Animales , Codón sin Sentido , Amplificación de Genes , Masculino , Familia de Multigenes , Elementos de Nucleótido Esparcido Corto , Sus scrofa , Porcinos/clasificación , Testículo/metabolismo , Factores de Transcripción/metabolismo
5.
Nature ; 462(7276): 1005-10, 2009 Dec 24.
Artículo en Inglés | MEDLINE | ID: mdl-20033038

RESUMEN

Multiple somatic rearrangements are often found in cancer genomes; however, the underlying processes of rearrangement and their contribution to cancer development are poorly characterized. Here we use a paired-end sequencing strategy to identify somatic rearrangements in breast cancer genomes. There are more rearrangements in some breast cancers than previously appreciated. Rearrangements are more frequent over gene footprints and most are intrachromosomal. Multiple rearrangement architectures are present, but tandem duplications are particularly common in some cancers, perhaps reflecting a specific defect in DNA maintenance. Short overlapping sequences at most rearrangement junctions indicate that these have been mediated by non-homologous end-joining DNA repair, although varying sequence patterns indicate that multiple processes of this type are operative. Several expressed in-frame fusion genes were identified but none was recurrent. The study provides a new perspective on cancer genomes, highlighting the diversity of somatic rearrangements and their potential contribution to cancer development.


Asunto(s)
Neoplasias de la Mama/genética , Aberraciones Cromosómicas , Reordenamiento Génico/genética , Genoma Humano/genética , Línea Celular Tumoral , Células Cultivadas , Roturas del ADN , Femenino , Biblioteca Genómica , Humanos , Análisis de Secuencia de ADN
6.
Nat Genet ; 38(7): 779-86, 2006 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-16804543

RESUMEN

We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.


Asunto(s)
Clostridioides difficile/genética , Clostridioides difficile/patogenicidad , Adaptación Fisiológica , Proteínas Bacterianas/genética , Secuencia de Bases , Clostridioides difficile/efectos de los fármacos , Clostridioides difficile/fisiología , Conjugación Genética , Elementos Transponibles de ADN/genética , ADN Bacteriano/genética , Farmacorresistencia Bacteriana Múltiple/genética , Enterocolitis Seudomembranosa/etiología , Enterocolitis Seudomembranosa/microbiología , Tracto Gastrointestinal/microbiología , Genoma Bacteriano , Humanos , Datos de Secuencia Molecular , Mosaicismo , Análisis de Secuencia por Matrices de Oligonucleótidos , Esporas Bacterianas/fisiología , Virulencia/genética
7.
PLoS Pathog ; 7(4): e1002018, 2011 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-21490962

RESUMEN

Citrobacter rodentium is a natural mouse pathogen that causes attaching and effacing (A/E) lesions. It shares a common virulence strategy with the clinically significant human A/E pathogens enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) and is widely used to model this route of pathogenesis. We previously reported the complete genome sequence of C. rodentium ICC168, where we found that the genome displayed many characteristics of a newly evolved pathogen. In this study, through PFGE, sequencing of isolates showing variation, whole genome transcriptome analysis and examination of the mobile genetic elements, we found that, consistent with our previous hypothesis, the genome of C. rodentium is unstable as a result of repeat-mediated, large-scale genome recombination and because of active transposition of mobile genetic elements such as the prophages. We sequenced an additional C. rodentium strain, EX-33, to reveal that the reference strain ICC168 is representative of the species and that most of the inactivating mutations were common to both isolates and likely to have occurred early on in the evolution of this pathogen. We draw parallels with the evolution of other bacterial pathogens and conclude that C. rodentium is a recently evolved pathogen that may have emerged alongside the development of inbred mice as a model for human disease.


Asunto(s)
Citrobacter rodentium/genética , Citrobacter rodentium/patogenicidad , Genoma Bacteriano , Animales , Citrobacter rodentium/clasificación , ADN Bacteriano/genética , Modelos Animales de Enfermedad , Electroforesis en Gel de Campo Pulsado , Infecciones por Enterobacteriaceae/patología , Femenino , Perfilación de la Expresión Génica , Reordenamiento Génico , Inestabilidad Genómica , Humanos , Secuencias Repetitivas Esparcidas , Ratones , Ratones Endogámicos C57BL , Plásmidos/genética , Profagos/genética , Análisis de Secuencia de ADN , Virulencia
8.
Proc Natl Acad Sci U S A ; 107(16): 7527-32, 2010 Apr 20.
Artículo en Inglés | MEDLINE | ID: mdl-20368420

RESUMEN

Clostridium difficile has rapidly emerged as the leading cause of antibiotic-associated diarrheal disease, with the transcontinental spread of various PCR ribotypes, including 001, 017, 027 and 078. However, the genetic basis for the emergence of C. difficile as a human pathogen is unclear. Whole genome sequencing was used to analyze genetic variation and virulence of a diverse collection of thirty C. difficile isolates, to determine both macro and microevolution of the species. Horizontal gene transfer and large-scale recombination of core genes has shaped the C. difficile genome over both short and long time scales. Phylogenetic analysis demonstrates C. difficile is a genetically diverse species, which has evolved within the last 1.1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages.


Asunto(s)
Clostridioides difficile/genética , Evolución Molecular , Biología Computacional , Regulación Bacteriana de la Expresión Génica , Técnicas de Transferencia de Gen , Genoma Bacteriano , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Polimorfismo de Nucleótido Simple , Recombinación Genética , Análisis de Secuencia de ADN , Especificidad de la Especie , Factores de Tiempo , Virulencia
9.
Nat Genet ; 35(1): 32-40, 2003 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-12910271

RESUMEN

Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica are closely related Gram-negative beta-proteobacteria that colonize the respiratory tracts of mammals. B. pertussis is a strict human pathogen of recent evolutionary origin and is the primary etiologic agent of whooping cough. B. parapertussis can also cause whooping cough, and B. bronchiseptica causes chronic respiratory infections in a wide range of animals. We sequenced the genomes of B. bronchiseptica RB50 (5,338,400 bp; 5,007 predicted genes), B. parapertussis 12822 (4,773,551 bp; 4,404 genes) and B. pertussis Tohama I (4,086,186 bp; 3,816 genes). Our analysis indicates that B. parapertussis and B. pertussis are independent derivatives of B. bronchiseptica-like ancestors. During the evolution of these two host-restricted species there was large-scale gene loss and inactivation; host adaptation seems to be a consequence of loss, not gain, of function, and differences in virulence may be related to loss of regulatory or control functions.


Asunto(s)
Bordetella bronchiseptica/genética , Bordetella pertussis/genética , Bordetella/genética , Genoma Bacteriano , Secuencia de Bases , Bordetella/metabolismo , Bordetella/patogenicidad , Bordetella bronchiseptica/metabolismo , Bordetella bronchiseptica/patogenicidad , Bordetella pertussis/metabolismo , Bordetella pertussis/patogenicidad , ADN Bacteriano , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Especificidad de la Especie
10.
J Bacteriol ; 194(6): 1494-504, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22247511

RESUMEN

Integrative and conjugative elements (ICEs) are self-mobile genetic elements found in the genomes of some bacteria. These elements may confer a fitness advantage upon their host bacteria through the cargo genes that they carry. Salmonella pathogenicity island 7 (SPI-7), found within some pathogenic strains of Salmonella enterica, possesses features indicative of an ICE and carries genes implicated in virulence. We aimed to identify and fully analyze ICEs related to SPI-7 within the genus Salmonella and other Enterobacteriaceae. We report the sequence of two novel SPI-7-like elements, found within strains of Salmonella bongori, which share 97% nucleotide identity over conserved regions with SPI-7 and with each other. Although SPI-7 within Salmonella enterica serovar Typhi appears to be fixed within the chromosome, we present evidence that these novel elements are capable of excision and self-mobility. Phylogenetic analyses show that these Salmonella mobile elements share an ancestor which existed approximately 3.6 to 15.8 million years ago. Additionally, we identified more distantly related ICEs, with distinct cargo regions, within other strains of Salmonella as well as within Citrobacter, Erwinia, Escherichia, Photorhabdus, and Yersinia species. In total, we report on a collection of 17 SPI-7 related ICEs within enterobacterial species, of which six are novel. Using comparative and mutational studies, we have defined a core of 27 genes essential for conjugation. We present a growing family of SPI-7-related ICEs whose mobility, abundance, and cargo variability indicate that these elements may have had a large impact on the evolution of the Enterobacteriaceae.


Asunto(s)
Proteínas Bacterianas/genética , Enterobacteriaceae/genética , Enterobacteriaceae/patogenicidad , Islas Genómicas , Secuencias Repetitivas Esparcidas , Factores de Virulencia/genética , Análisis por Conglomerados , Análisis Mutacional de ADN , ADN Bacteriano/química , ADN Bacteriano/genética , Evolución Molecular , Transferencia de Gen Horizontal , Datos de Secuencia Molecular , Filogenia , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico
11.
BMC Microbiol ; 11: 7, 2011 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-21219646

RESUMEN

BACKGROUND: The gut microbiota is thought to play a key role in the development of the inflammatory bowel diseases Crohn's disease (CD) and ulcerative colitis (UC). Shifts in the composition of resident bacteria have been postulated to drive the chronic inflammation seen in both diseases (the "dysbiosis" hypothesis). We therefore specifically sought to compare the mucosa-associated microbiota from both inflamed and non-inflamed sites of the colon in CD and UC patients to that from non-IBD controls and to detect disease-specific profiles. RESULTS: Paired mucosal biopsies of inflamed and non-inflamed intestinal tissue from 6 CD (n = 12) and 6 UC (n = 12) patients were compared to biopsies from 5 healthy controls (n = 5) by in-depth sequencing of over 10,000 near full-length bacterial 16S rRNA genes. The results indicate that mucosal microbial diversity is reduced in IBD, particularly in CD, and that the species composition is disturbed. Firmicutes were reduced in IBD samples and there were concurrent increases in Bacteroidetes, and in CD only, Enterobacteriaceae. There were also significant differences in microbial community structure between inflamed and non-inflamed mucosal sites. However, these differences varied greatly between individuals, meaning there was no obvious bacterial signature that was positively associated with the inflamed gut. CONCLUSIONS: These results may support the hypothesis that the overall dysbiosis observed in inflammatory bowel disease patients relative to non-IBD controls might to some extent be a result of the disturbed gut environment rather than the direct cause of disease. Nonetheless, the observed shifts in microbiota composition may be important factors in disease maintenance and severity.


Asunto(s)
Colitis Ulcerosa/microbiología , Colon/microbiología , Enfermedad de Crohn/microbiología , Biblioteca de Genes , Mucosa Intestinal/microbiología , Metagenoma , Adulto , Anciano , Carga Bacteriana , Biopsia , Estudios de Casos y Controles , Enterobacteriaceae/clasificación , Enterobacteriaceae/genética , Femenino , Humanos , Masculino , Persona de Mediana Edad , ARN Bacteriano/genética , ARN Ribosómico 16S/genética , Adulto Joven
12.
Nature ; 433(7028): 865-8, 2005 Feb 24.
Artículo en Inglés | MEDLINE | ID: mdl-15729342

RESUMEN

Entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries. Here we present the genome of E. histolytica, which reveals a variety of metabolic adaptations shared with two other amitochondrial protist pathogens: Giardia lamblia and Trichomonas vaginalis. These adaptations include reduction or elimination of most mitochondrial metabolic pathways and the use of oxidative stress enzymes generally associated with anaerobic prokaryotes. Phylogenomic analysis identifies evidence for lateral gene transfer of bacterial genes into the E. histolytica genome, the effects of which centre on expanding aspects of E. histolytica's metabolic repertoire. The presence of these genes and the potential for novel metabolic pathways in E. histolytica may allow for the development of new chemotherapeutic agents. The genome encodes a large number of novel receptor kinases and contains expansions of a variety of gene families, including those associated with virulence. Additional genome features include an abundance of tandemly repeated transfer-RNA-containing arrays, which may have a structural function in the genome. Analysis of the genome provides new insights into the workings and genome evolution of a major human pathogen.


Asunto(s)
Entamoeba histolytica/genética , Genoma de Protozoos , Parásitos/genética , Animales , Entamoeba histolytica/metabolismo , Entamoeba histolytica/patogenicidad , Evolución Molecular , Fermentación , Transferencia de Gen Horizontal/genética , Glucólisis , Estrés Oxidativo/genética , Parásitos/metabolismo , Parásitos/patogenicidad , Filogenia , Transducción de Señal , Virulencia/genética
13.
BMC Genomics ; 11: 438, 2010 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-20642822

RESUMEN

BACKGROUND: The pig genome is being sequenced and characterised under the auspices of the Swine Genome Sequencing Consortium. The sequencing strategy followed a hybrid approach combining hierarchical shotgun sequencing of BAC clones and whole genome shotgun sequencing. RESULTS: Assemblies of the BAC clone derived genome sequence have been annotated using the Pre-Ensembl and Ensembl automated pipelines and made accessible through the Pre-Ensembl/Ensembl browsers. The current annotated genome assembly (Sscrofa9) was released with Ensembl 56 in September 2009. A revised assembly (Sscrofa10) is under construction and will incorporate whole genome shotgun sequence (WGS) data providing > 30x genome coverage. The WGS sequence, most of which comprise short Illumina/Solexa reads, were generated from DNA from the same single Duroc sow as the source of the BAC library from which clones were preferentially selected for sequencing. In accordance with the Bermuda and Fort Lauderdale agreements and the more recent Toronto Statement the data have been released into public sequence repositories (Genbank/EMBL, NCBI/Ensembl trace repositories) in a timely manner and in advance of publication. CONCLUSIONS: In this marker paper, the Swine Genome Sequencing Consortium (SGSC) sets outs its plans for analysis of the pig genome sequence, for the application and publication of the results.


Asunto(s)
Genoma , Sus scrofa/genética , Animales , Publicaciones , Análisis de Secuencia de ADN
14.
Ann Surg ; 252(1): 90-8, 2010 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-20562611

RESUMEN

OBJECTIVE: To identify, compare, and contrast the microbiota in patients with and without pouchitis after restorative proctocolectomy (RPC) for ulcerative colitis (UC) and familial adenomatous polyposis (FAP). SUMMARY BACKGROUND DATA: Pouchitis is the most common complication following RPC. An abnormal host-microbial interaction has been implicated. We investigated the pouch microbiota in patients with and without pouchitis undergoing restorative proctocolectomy for UC and FAP. METHODS: Mucosal pouch biopsies, taken from 16 UC (pouchitis 8) and 8 FAP (pouchitis 3) patients were analyzed to the species (or phylotype) level by cloning and sequencing of 3184 full-length bacterial 16S rRNA genes. RESULTS: There was a significant increase in Proteobacteria (P = 0.019) and a significant decrease in Bacteroidetes (P = 0.001) and Faecalibacterium prausnitzii (P = 0.029) in the total UC compared with the total FAP cohort, but only limited differences were found between the UC nonpouchitis and pouchitis groups and the FAP pouchitis and nonpouchitis groups. Bacterial diversity in the FAP nonpouchitis group was significantly greater than in UC nonpouchitis (P = 0.019) and significantly greater in UC nonpouchitis compared with UC pouchitis (P = 0.009). No individual species or phylotype specifically associated with either UC or FAP pouchitis was found. CONCLUSIONS: UC pouch patients have a different, less diverse, gut microbiota than FAP patients. A further reduction in bacterial diversity but no significant dysbiosis occurs in those with pouchitis. The study suggests that a dysbiosis occurs in the ileal pouch of UC RPC patients which predisposes to, but may not directly cause, pouchitis.


Asunto(s)
Reservoritis/microbiología , ARN Ribosómico 16S/genética , Poliposis Adenomatosa del Colon/microbiología , Poliposis Adenomatosa del Colon/cirugía , Adulto , Bacteroidetes/genética , Bacteroidetes/aislamiento & purificación , Secuencia de Bases , Biopsia , Clonación Molecular , Colitis Ulcerosa/microbiología , Colitis Ulcerosa/cirugía , Reservorios Cólicos/microbiología , ADN Bacteriano/análisis , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Proctocolectomía Restauradora , Proteobacteria/genética , Proteobacteria/aislamiento & purificación
15.
PLoS Genet ; 3(2): e23, 2007 Feb 16.
Artículo en Inglés | MEDLINE | ID: mdl-17305430

RESUMEN

The bacterium Neisseria meningitidis is commonly found harmlessly colonising the mucosal surfaces of the human nasopharynx. Occasionally strains can invade host tissues causing septicaemia and meningitis, making the bacterium a major cause of morbidity and mortality in both the developed and developing world. The species is known to be diverse in many ways, as a product of its natural transformability and of a range of recombination and mutation-based systems. Previous work on pathogenic Neisseria has identified several mechanisms for the generation of diversity of surface structures, including phase variation based on slippage-like mechanisms and sequence conversion of expressed genes using information from silent loci. Comparison of the genome sequences of two N. meningitidis strains, serogroup B MC58 and serogroup A Z2491, suggested further mechanisms of variation, including C-terminal exchange in specific genes and enhanced localised recombination and variation related to repeat arrays. We have sequenced the genome of N. meningitidis strain FAM18, a representative of the ST-11/ET-37 complex, providing the first genome sequence for the disease-causing serogroup C meningococci; it has 1,976 predicted genes, of which 60 do not have orthologues in the previously sequenced serogroup A or B strains. Through genome comparison with Z2491 and MC58 we have further characterised specific mechanisms of genetic variation in N. meningitidis, describing specialised loci for generation of cell surface protein variants and measuring the association between noncoding repeat arrays and sequence variation in flanking genes. Here we provide a detailed view of novel genetic diversification mechanisms in N. meningitidis. Our analysis provides evidence for the hypothesis that the noncoding repeat arrays in neisserial genomes (neisserial intergenic mosaic elements) provide a crucial mechanism for the generation of surface antigen variants. Such variation will have an impact on the interaction with the host tissues, and understanding these mechanisms is important to aid our understanding of the intimate and complex relationship between the human nasopharynx and the meningococcus.


Asunto(s)
Variación Genética , Neisseria meningitidis Serogrupo C/genética , Proteínas Bacterianas/genética , Composición de Base/genética , Reordenamiento Génico , Genes Bacterianos , Humanos , Análisis de Secuencia por Matrices de Oligonucleótidos , Sistemas de Lectura Abierta/genética , Secuencias Repetitivas de Ácidos Nucleicos/genética , Homología de Secuencia de Ácido Nucleico , Sintenía/genética
16.
J Bacteriol ; 191(1): 261-77, 2009 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-18931103

RESUMEN

Bacterial infections of the lungs of cystic fibrosis (CF) patients cause major complications in the treatment of this common genetic disease. Burkholderia cenocepacia infection is particularly problematic since this organism has high levels of antibiotic resistance, making it difficult to eradicate; the resulting chronic infections are associated with severe declines in lung function and increased mortality rates. B. cenocepacia strain J2315 was isolated from a CF patient and is a member of the epidemic ET12 lineage that originated in Canada or the United Kingdom and spread to Europe. The 8.06-Mb genome of this highly transmissible pathogen comprises three circular chromosomes and a plasmid and encodes a broad array of functions typical of this metabolically versatile genus, as well as numerous virulence and drug resistance functions. Although B. cenocepacia strains can be isolated from soil and can be pathogenic to both plants and man, J2315 is representative of a lineage of B. cenocepacia rarely isolated from the environment and which spreads between CF patients. Comparative analysis revealed that ca. 21% of the genome is unique in comparison to other strains of B. cenocepacia, highlighting the genomic plasticity of this species. Pseudogenes in virulence determinants suggest that the pathogenic response of J2315 may have been recently selected to promote persistence in the CF lung. The J2315 genome contains evidence that its unique and highly adapted genetic content has played a significant role in its success as an epidemic CF pathogen.


Asunto(s)
Complejo Burkholderia cepacia/genética , Complejo Burkholderia cepacia/patogenicidad , Burkholderia/genética , Burkholderia/patogenicidad , Fibrosis Quística/microbiología , Genoma Bacteriano , Complejo Burkholderia cepacia/efectos de los fármacos , Complejo Burkholderia cepacia/aislamiento & purificación , Mapeo Cromosómico , Cromosomas Bacterianos/genética , Cartilla de ADN , ADN Bacteriano/genética , ADN Circular/genética , Farmacorresistencia Microbiana , Amplificación de Genes , Humanos , Plantas/microbiología , Plásmidos , Reacción en Cadena de la Polimerasa , Esputo/microbiología
17.
BMC Genomics ; 10: 302, 2009 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-19583835

RESUMEN

BACKGROUND: The Gram-negative bacterium Photorhabdus asymbiotica (Pa) has been recovered from human infections in both North America and Australia. Recently, Pa has been shown to have a nematode vector that can also infect insects, like its sister species the insect pathogen P. luminescens (Pl). To understand the relationship between pathogenicity to insects and humans in Photorhabdus we have sequenced the complete genome of Pa strain ATCC43949 from North America. This strain (formerly referred to as Xenorhabdus luminescens strain 2) was isolated in 1977 from the blood of an 80 year old female patient with endocarditis, in Maryland, USA. Here we compare the complete genome of Pa ATCC43949 with that of the previously sequenced insect pathogen P. luminescens strain TT01 which was isolated from its entomopathogenic nematode vector collected from soil in Trinidad and Tobago. RESULTS: We found that the human pathogen Pa had a smaller genome (5,064,808 bp) than that of the insect pathogen Pl (5,688,987 bp) but that each pathogen carries approximately one megabase of DNA that is unique to each strain. The reduced size of the Pa genome is associated with a smaller diversity in insecticidal genes such as those encoding the Toxin complexes (Tc's), Makes caterpillars floppy (Mcf) toxins and the Photorhabdus Virulence Cassettes (PVCs). The Pa genome, however, also shows the addition of a plasmid related to pMT1 from Yersinia pestis and several novel pathogenicity islands including a novel Type Three Secretion System (TTSS) encoding island. Together these data suggest that Pa may show virulence against man via the acquisition of the pMT1-like plasmid and specific effectors, such as SopB, that promote its persistence inside human macrophages. Interestingly the loss of insecticidal genes in Pa is not reflected by a loss of pathogenicity towards insects. CONCLUSION: Our results suggest that North American isolates of Pa have acquired virulence against man via the acquisition of a plasmid and specific virulence factors with similarity to those shown to play roles in pathogenicity against humans in other bacteria.


Asunto(s)
Hibridación Genómica Comparativa , Genoma Bacteriano , Photorhabdus/genética , Photorhabdus/patogenicidad , Animales , Línea Celular , Enfermedades Transmisibles Emergentes/microbiología , ADN Bacteriano/genética , Infecciones por Enterobacteriaceae/microbiología , Islas Genómicas , Genómica , Humanos , Ratones , Mariposas Nocturnas/microbiología , América del Norte , Photorhabdus/aislamiento & purificación , Plásmidos , Análisis de Secuencia de ADN , Especificidad de la Especie , Virulencia
18.
PLoS Genet ; 2(12): e206, 2006 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-17173484

RESUMEN

The human enteropathogen, Yersinia enterocolitica, is a significant link in the range of Yersinia pathologies extending from mild gastroenteritis to bubonic plague. Comparison at the genomic level is a key step in our understanding of the genetic basis for this pathogenicity spectrum. Here we report the genome of Y. enterocolitica strain 8081 (serotype 0:8; biotype 1B) and extensive microarray data relating to the genetic diversity of the Y. enterocolitica species. Our analysis reveals that the genome of Y. enterocolitica strain 8081 is a patchwork of horizontally acquired genetic loci, including a plasticity zone of 199 kb containing an extraordinarily high density of virulence genes. Microarray analysis has provided insights into species-specific Y. enterocolitica gene functions and the intraspecies differences between the high, low, and nonpathogenic Y. enterocolitica biotypes. Through comparative genome sequence analysis we provide new information on the evolution of the Yersinia. We identify numerous loci that represent ancestral clusters of genes potentially important in enteric survival and pathogenesis, which have been lost or are in the process of being lost, in the other sequenced Yersinia lineages. Our analysis also highlights large metabolic operons in Y. enterocolitica that are absent in the related enteropathogen, Yersinia pseudotuberculosis, indicating major differences in niche and nutrients used within the mammalian gut. These include clusters directing, the production of hydrogenases, tetrathionate respiration, cobalamin synthesis, and propanediol utilisation. Along with ancestral gene clusters, the genome of Y. enterocolitica has revealed species-specific and enteropathogen-specific loci. This has provided important insights into the pathology of this bacterium and, more broadly, into the evolution of the genus. Moreover, wider investigations looking at the patterns of gene loss and gain in the Yersinia have highlighted common themes in the genome evolution of other human enteropathogens.


Asunto(s)
Genoma Bacteriano , Yersinia enterocolitica/genética , Evolución Molecular , Genómica , Análisis por Micromatrices , Datos de Secuencia Molecular , Yersinia enterocolitica/clasificación , Yersinia enterocolitica/patogenicidad
19.
J Bacteriol ; 190(11): 4027-37, 2008 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-18375554

RESUMEN

The gram-negative enteric bacterium Proteus mirabilis is a frequent cause of urinary tract infections in individuals with long-term indwelling catheters or with complicated urinary tracts (e.g., due to spinal cord injury or anatomic abnormality). P. mirabilis bacteriuria may lead to acute pyelonephritis, fever, and bacteremia. Most notoriously, this pathogen uses urease to catalyze the formation of kidney and bladder stones or to encrust or obstruct indwelling urinary catheters. Here we report the complete genome sequence of P. mirabilis HI4320, a representative strain cultured in our laboratory from the urine of a nursing home patient with a long-term (> or =30 days) indwelling urinary catheter. The genome is 4.063 Mb long and has a G+C content of 38.88%. There is a single plasmid consisting of 36,289 nucleotides. Annotation of the genome identified 3,685 coding sequences and seven rRNA loci. Analysis of the sequence confirmed the presence of previously identified virulence determinants, as well as a contiguous 54-kb flagellar regulon and 17 types of fimbriae. Genes encoding a potential type III secretion system were identified on a low-G+C-content genomic island containing 24 intact genes that appear to encode all components necessary to assemble a type III secretion system needle complex. In addition, the P. mirabilis HI4320 genome possesses four tandem copies of the zapE metalloprotease gene, genes encoding six putative autotransporters, an extension of the atf fimbrial operon to six genes, including an mrpJ homolog, and genes encoding at least five iron uptake mechanisms, two potential type IV secretion systems, and 16 two-component regulators.


Asunto(s)
Adhesión Bacteriana/genética , Genoma Bacteriano , Proteus mirabilis/genética , Animales , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Quimiotaxis/genética , Cromosomas Bacterianos , Femenino , Fimbrias Bacterianas/genética , Regulación Bacteriana de la Expresión Génica/fisiología , Humanos , Ratones , Ratones Endogámicos CBA , Datos de Secuencia Molecular , Movimiento/fisiología , Plásmidos/genética , Infecciones por Proteus/microbiología , Proteus mirabilis/patogenicidad , Proteus mirabilis/fisiología , Infecciones Urinarias/microbiología , Virulencia/genética , Factores de Virulencia/genética
20.
BMC Genomics ; 9: 616, 2008 Dec 19.
Artículo en Inglés | MEDLINE | ID: mdl-19099551

RESUMEN

BACKGROUND: The fish pathogen Aliivibrio salmonicida is the causative agent of cold-water vibriosis in marine aquaculture. The Gram-negative bacterium causes tissue degradation, hemolysis and sepsis in vivo. RESULTS: In total, 4 286 protein coding sequences were identified, and the 4.6 Mb genome of A. salmonicida has a six partite architecture with two chromosomes and four plasmids. Sequence analysis revealed a highly fragmented genome structure caused by the insertion of an extensive number of insertion sequence (IS) elements. The IS elements can be related to important evolutionary events such as gene acquisition, gene loss and chromosomal rearrangements. New A. salmonicida functional capabilities that may have been aquired through horizontal DNA transfer include genes involved in iron-acquisition, and protein secretion and play potential roles in pathogenicity. On the other hand, the degeneration of 370 genes and consequent loss of specific functions suggest that A. salmonicida has a reduced metabolic and physiological capacity in comparison to related Vibrionaceae species. CONCLUSION: Most prominent is the loss of several genes involved in the utilisation of the polysaccharide chitin. In particular, the disruption of three extracellular chitinases responsible for enzymatic breakdown of chitin makes A. salmonicida unable to grow on the polymer form of chitin. These, and other losses could restrict the variety of carrier organisms A. salmonicida can attach to, and associate with. Gene acquisition and gene loss may be related to the emergence of A. salmonicida as a fish pathogen.


Asunto(s)
Aliivibrio salmonicida/genética , Peces/microbiología , Genoma Bacteriano , Animales , Cromosomas Bacterianos/genética , Elementos Transponibles de ADN , ADN Bacteriano/genética , Genómica , Plásmidos/genética , Alineación de Secuencia , Análisis de Secuencia de ADN
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