An integrative genomic approach identifies p73 and p63 as activators of miR-200 microRNA family transcription.

Although microRNAs (miRNAs) are important regulators of gene expression, the transcriptional regulation of miRNAs themselves is not well understood. We employed an integrative computational pipeline to dissect the transcription factors (TFs) responsible for altered miRNA expression in ovarian carcinoma. Using experimental data and computational predictions to define miRNA promoters across the human genome, we identified TFs with binding sites significantly overrepresented among miRNA genes overexpressed in ovarian carcinoma. This pipeline nominated TFs of the p53/p63/p73 family as candidate drivers of miRNA overexpression. Analysis of data from an independent set of 253 ovarian carcinomas in The Cancer Genome Atlas showed that p73 and p63 expression is significantly correlated with expression of miRNAs whose promoters contain p53/p63/p73 family binding sites. In experimental validation of specific miRNAs predicted by the analysis to be regulated by p73 and p63, we found that p53/p63/p73 family binding sites modulate promoter activity of miRNAs of the miR-200 family, which are known regulators of cancer stem cells and epithelial-mesenchymal transitions. Furthermore, in chromatin immunoprecipitation studies both p73 and p63 directly associated with the miR-200b/a/429 promoter. This study delineates an integrative approach that can be applied to discover transcriptional regulatory mechanisms in other biological settings where analogous genomic data are available.

Identification and characterization of compounds from Chrysosporium multifidum, a fungus with moderate antimicrobial activity isolated from Hermetia illucens gut microbiota.

The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of Hermetia illucens larvae. Extract from C. multifidum culture broth supernatant showed moderate activity against a strain of methicillin-resistant Staphylococcus aureus (MRSA). Bioguided isolation of the extract resulted in the characterization of six α-pyrone derivatives (1-6) and one diketopiperazine (7). Of these compounds, 5,6-dihydro-4-methoxy-6-(1-oxopentyl)-2H-pyran-2-one (4) showed the greatest activity (IC50 = 11.4 ± 0.7 µg/mL and MIC = 62.5 µg/mL) against MRSA.

Barreras de acceso del sistema de salud colombiano en zonas rurales: percepciones de usuarios del régimen subsidiado / Access barriers to the colombian health system in rural areas: perceptions of users of the subsidized regime / Barreiras de aceso do sistema de saúde colombiano em zonas rurais: percepções de usuarios do (régime subsidiado) no brasil (sus) sistema único de saúde

Objetivo: Explorar la percepción las barreras de acceso a los servicios de salud percibidas por usuarios del régimen subsidiado del municipio de Sonsón para el año 2016. Metodología: se realizó una investigación cualitativa con enfoque histórico hermenéutico, donde participaron 16 informantes, quienes fueron entrevistados y sus relatos transcritos, codificados y analizados con base en la teoría fundamentada. Resultados: Se identificaron barreras económicas y administrativas que limitan el acceso de las personas a los servicios de salud; representadas en las demoras para reclamar medicamentos y su dispensación incompleta, lo que lleva a que las personas asuman gastos de bolsillo o desistan del tratamiento. Conclusiones: estas barreras son más recalcitrantes en poblaciones pobres y vulnerables, pues su condición coarta sus posibilidades para hacer frente a las eventualidades derivadas de las barreras existentes en el sistema.

Objective: To explore the perception users of the subsidized regime of the municipality of Sonsón, Colombia had in 2016 about the barriers to the access to health services. Methods: Qualitative research with a hermeneutic historical approach with the participation of 16 informants who were interviewed and their stories were transcribed, codified and analyzed based on the Grounded Theory. Results: Economic and administrative barriers that limit the access of people to health services were identified represented by delays in claiming medicines and their incomplete distribution, leading people to assume out-of-pocket costs or giving up on treatment. Conclusions: These barriers are more recalcitrant in poor and vulnerable populations since their condition limits their possibilities to face the eventualities derived from the existing barriers in the system.

Objetivo: Explorar a percepção às barreiras de acesso aos serviços de saúde percebidas por usuários do (Regímen subsidiado) Sistema único de saúde do município de Sonsón para o ano 2016. Metodologia: Realizou-se uma pesquisa qualitativo com enfoque histórico hermenêutico, onde participaram 16 informantes, quem foram entrevistados e seus relatos transcritos, codificados e analisados com base na teoria fundamentada. Resultados: Identificaram-se barreiras econômicas e administrativas que limitam o acesso das pessoas aos serviços de saúde; representadas nas demoras para obter medicamentos e sua dispensação incompleta, o que leva a que as pessoas assumam gastos básicos ou desistam do tratamento. Conclusões: estas barreiras são mais recalcitrantes em população pobre e vulnerável, já que sua condição coarcta suas possibilidades para fazer frente às eventualidades derivadas das barreiras existentes no sistema

Barreras de acceso del sistema de salud colombiano en zonas rurales: percepciones de usuarios del régimen subsidiado / Access barriers to the colombian health system in rural areas: perceptions of users of the subsidized regime / Barreiras de aceso do sistema de saúde colombiano em zonas rurais: percepções de usuarios do (régime subsidiado) no brasil (sus) sistema único de saúde

Resumen Objetivo: Explorar la percepción las barreras de acceso a los servicios de salud percibidas por usuarios del régimen subsidiado del municipio de Sonsón para el año 2016. Metodología: se realizó una investigación cualitativa con enfoque histórico hermenéutico, donde participaron 16 informantes, quienes fueron entrevistados y sus relatos transcritos, codificados y analizados con base en la teoría fundamentada. Resultados: Se identificaron barreras económicas y administrativas que limitan el acceso de las personas a los servicios de salud; representadas en las demoras para reclamar medicamentos y su dispensación incompleta, lo que lleva a que las personas asuman gastos de bolsillo o desistan del tratamiento. Conclusiones: estas barreras son más recalcitrantes en poblaciones pobres y vulnerables, pues su condición coarta sus posibilidades para hacer frente a las eventualidades derivadas de las barreras existentes en el sistema.

Abstract Objective: To explore the perception users of the subsidized regime of the municipality of Sonsón, Colombia had in 2016 about the barriers to the access to health services. Methods: Qualitative research with a hermeneutic historical approach with the participation of 16 informants who were interviewed and their stories were transcribed, codified and analyzed based on the Grounded Theory. Results: Economic and administrative barriers that limit the access of people to health services were identified represented by delays in claiming medicines and their incomplete distribution, leading people to assume out-of-pocket costs or giving up on treatment. Conclusions: These barriers are more recalcitrant in poor and vulnerable populations since their condition limits their possibilities to face the eventualities derived from the existing barriers in the system.

Resumo Objetivo: Explorar a percepção às barreiras de acesso aos serviços de saúde percebidas por usuários do (Regímen subsidiado) Sistema único de saúde do município de Sonsón para o ano 2016. Metodologia: Realizou-se uma pesquisa qualitativo com enfoque histórico hermenêutico, onde participaram 16 informantes, quem foram entrevistados e seus relatos transcritos, codificados e analisados com base na teoria fundamentada. Resultados: Identificaram-se barreiras econômicas e administrativas que limitam o acesso das pessoas aos serviços de saúde; representadas nas demoras para obter medicamentos e sua dispensação incompleta, o que leva a que as pessoas assumam gastos básicos ou desistam do tratamento. Conclusões: estas barreiras são mais recalcitrantes em população pobre e vulnerável, já que sua condição coarcta suas possibilidades para fazer frente às eventualidades derivadas das barreiras existentes no sistema.