RESUMEN
Angiotensin converting enzyme (ACE) activity contributes to the vascular response to injury because ACE inhibition limits neointima formation in rat carotid arteries after balloon injury. To investigate the mechanisms by which ACE may contribute to vascular smooth muscle cell (VSMC) proliferation, we studied expression of ACE in vivo after injury and in vitro after growth factor stimulation. ACE activity 14 d after injury was increased 3.6-fold in the injured vessel. ACE expression, measured by immunohistochemistry, became apparent at 7 d in the neointima and at 14 d was primarily in the most luminal neointimal cells. To characterize hormones that induce ACE in vivo, cultured VSMC were exposed to steroids and growth factors. Among steroids, only glucocorticoids stimulated ACE expression with an 8.0 +/- 2.1-fold increase in activity and a 6.5-fold increase in mRNA (30 nM dexamethasone for 72 h). Among growth factors tested, only fibroblast growth factor (FGF) stimulated ACE expression (4.2 +/- 0.7-fold increase in activity and 1.6-fold increase in mRNA in response to 10 ng/ml FGF for 24 h). Dexamethasone and FGF were synergistic at the indicated concentrations inducing 50.6 +/- 12.4-fold and 32.5-fold increases in activity and mRNA expression, respectively. In addition, when porcine iliac arteries were transfected with recombinant FGF-1 (in the absence of injury), ACE expression increased in neointimal VSMC, to the same extent as injured, nontransfected arteries. The data suggest a temporal sequence for the response to injury in which FGF induces ACE, ACE generates angiotensin II, and angiotensin II stimulates VSMC growth in concert with FGF.
Asunto(s)
Arterias/efectos de los fármacos , Factores de Crecimiento de Fibroblastos/farmacología , Regulación de la Expresión Génica , Músculo Liso Vascular/efectos de los fármacos , Peptidil-Dipeptidasa A/biosíntesis , Animales , Aorta/citología , Aorta/patología , Arterias/citología , Arterias/patología , Arterias Carótidas/efectos de los fármacos , Arterias Carótidas/patología , Células Cultivadas , Relación Dosis-Respuesta a Droga , Inducción Enzimática , Factores de Crecimiento de Fibroblastos/genética , Glucocorticoides , Masculino , Músculo Liso Vascular/enzimología , Músculo Liso Vascular/patología , Ratas , Proteínas Recombinantes/farmacología , Porcinos , TransfecciónRESUMEN
Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [3H]-benzo[alpha]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[alpha]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[alpha]pyrene metabolites are presented.
Asunto(s)
Benzopirenos/metabolismo , Macrófagos/metabolismo , Alveolos Pulmonares/metabolismo , Adulto , Células Cultivadas , Humanos , Neoplasias Pulmonares/metabolismoRESUMEN
We propose a model for signaling events induced by fluid shear stress that incorporates many of the features discussed in this paper (FIG. 4). First, heterotrimeric G-proteins, as well as a small G-proteins, are activated by flow. Indeed, a G protein appears to be required for ERK1/2 activation by flow because ERK1/2 activation is completely inhibited by GDP-beta S. Then, flow activates phospholipase C and generates IP3 and diacylglycerol (DG). IP3 releases Ca2+ from internal Ca2+ stores via IP3 receptor and DG activates PKC. Nollert and colleagues have shown that flow activates PLC and increases IP3. It is possible that several different PKC isozymes are activated by flow including both Ca(2+)-dependent and Ca(2+)-independent isozymes. These different isozymes may have specific downstream substrates. For example, PKC-epsilon may be involved in activation of ERK1/2, while the PKC isozyme responsible for activation of JNK remains unknown. It is also possible that these PKC isozymes may be important in gene transcription events. For example, PKC-zeta has been suggested to be involved in NF-kappa B-mediated gene transcription. Longer term changes in endothelial cell morphology and structure are likely to involve separate kinases. Important candidates for these changes include members of the c-Src and FAK families. c-Src is now considered to be a component of the focal adhesion complex and regulate focal adhesion formation and/or cytoskeletal rearrangement. Recently, stretch, another mechanostress, has been shown to activate c-Src in fetal rat lung cells. It has been clarified that ERK1/2 and JNK are regulated by the small G-proteins, Ras and Rac/Cdc42H, respectively, and their effectors in parallel with each other. Rac and Rho are also thought to be involved in membrane ruffling and/or cytoskeletal rearrangement. Fluid shear stress causes stress fiber formation and focal adhesion rearrangement. Recent study by Malek and Izumo suggested the importance of microtubules in shear stress-induced morphological change and actin stress fiber formation. It is clear that the focal adhesion complex plays an important role in shear stress-induced signal and it is interesting to speculate that shear stress-induced signaling has cross-talk with signaling induced by integrins. As a general model we propose that the integration between the rapid events stimulated by shear stress and the longer term events is mediated by tyrosine kinases that serve to regulate these multiple signal transduction pathways.
Asunto(s)
Endotelio Vascular/fisiopatología , Transducción de Señal , Animales , Arteriosclerosis/patología , Arteriosclerosis/fisiopatología , Endotelio Vascular/patología , Humanos , Estrés MecánicoRESUMEN
Fluid shear stress regulates endothelial cell function, but the signal transduction mechanisms involved in mechanotransduction remain unclear. Recent findings demonstrate that several intracellular kinases are activated by mechanical forces. In particular, members of the mitogen-activated protein (MAP) kinase family are stimulated by hyperosmolarity, stretch, and stress such as heat shock. We propose a model for mechanotransduction in endothelial cells involving calcium-dependent and calcium-independent protein kinase pathways. The calcium-dependent pathway involves activation of phospholipase C, hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), increases in intracellular calcium and stimulation of kinases such as calcium-calmodulin and C kinases (PKC). The calcium-independent pathway involves activation of a small GTP-binding protein and stimulation of calcium-independent PKC and MAP kinases. The calcium-dependent pathway mediates the rapid, transient response to fluid shear stress including activation of nitric oxide synthase (NOS) and ion transport. In contrast, the calcium-independent pathway mediates a slower response including the sustained activation of NOS and changes in cell morphology and gene expression. We propose that focal adhesion complexes link the calcium-dependent and calcium-independent pathways by regulating activity of phosphatidylinositol 4-phosphate (PIP) 5-kinase (which regulates PIP2 levels) and p125 focal adhesion kinase (FAK, which phosphorylates paxillin and interacts with cytoskeletal proteins). This model predicts that dynamic interactions between integrin molecules present in focal adhesion complexes and membrane events involved in mechanotransduction will be integrated by calcium-dependent and calcium-independent kinases to generate intracellular signals involved in the endothelial cell response to flow.
Asunto(s)
Calcio/fisiología , Endotelio Vascular/fisiología , Proteínas Quinasas/fisiología , Transducción de Señal/fisiología , Calmodulina/fisiología , Moléculas de Adhesión Celular/fisiología , Proteínas del Citoesqueleto/fisiología , Endotelio Vascular/citología , Quinasa 1 de Adhesión Focal , Proteína-Tirosina Quinasas de Adhesión Focal , Proteínas de Unión al GTP/fisiología , Expresión Génica , Humanos , Integrinas/fisiología , Transporte Iónico , Modelos Cardiovasculares , Óxido Nítrico Sintasa/fisiología , Concentración Osmolar , Paxillin , Fosfatidilinositol 4,5-Difosfato , Fosfatos de Fosfatidilinositol/fisiología , Fosfoproteínas/fisiología , Proteína Quinasa C/fisiología , Proteínas Tirosina Quinasas/fisiología , Receptor de Insulina/fisiología , Reología , Estrés Mecánico , Estrés Fisiológico/fisiopatología , Fosfolipasas de Tipo C/fisiologíaRESUMEN
A case of angina bullosa haemorrhagica is reported in which blood blisters appeared during crown preparation. The aetiology, differential diagnosis and management are discussed. Practitioners should be aware of this disorder and take precautions to minimise trauma during operative procedures.
Asunto(s)
Vesícula/etiología , Coronas/efectos adversos , Preparación de la Cavidad Dental/efectos adversos , Mucosa Bucal/lesiones , Hemorragia Bucal/etiología , Adulto , Diagnóstico Diferencial , Femenino , Estudios de Seguimiento , Humanos , Enfermedades de los Labios/etiología , Úlcera/etiologíaRESUMEN
An understanding of a broader concept of health is increasingly important for all health professionals, including dentists, and has recently been incorporated as a key principle in the Government White Paper, The New NHS. This aims to deliver a dependable, high quality, egalitarian health service. In the past, performance measurements in the UK have often relied simply on those areas which are most easily quantified. For example, within the hospital service, performance was measured in terms of the cost and the number of finished consultant episodes, from which the 'purchaser efficiency index' was calculated. This tended to produce a driving force rewarding those doing more rather than those doing more better. It is analogous to the system which has been the backbone of NHS dental practice for many years, 'fee per item of service', where throughout is rewarded rather than outcome. However, the White Paper has signalled a move away from simply counting activity. From April 1999 within the hospital service the purchaser efficiency index has been replaced with more rounded measures, reflecting the changing concepts of health, in a new broader performance framework to determine what really counts for patients. It will focus on measuring health improvement, fairer access, better quality and outcome, including the views of patients.
Asunto(s)
Atención Odontológica , Salud Bucal , Indicadores de Salud , Humanos , Calidad de Vida , Odontología Estatal , Medicina Estatal , Reino UnidoRESUMEN
The vascular tree is a highly specialized organ that has developed a complex and highly orchestrated mechanism of response to injury and stress. After PTCA-like injury many of these mechanisms are activated to maintain vessel integrity. Similar but clearly different growth mechanisms are activated by hypertension and hemodynamic stress. In this light, restenosis is merely a reparative response that has gone on too long or to a greater extent than necessary. The number of growth factors that affect this system is enormous, and their temporal and spatial distribution suggests that therapy targeted to any single factor is unlikely to be successful. In contrast, therapies directed at common mediators such as extracellular matrix and downstream cellular effectors may be more effective. Increasing knowledge of these interactions should point the way to new therapies based on cell-cell and cell-matrix interactions that are tissue specific and focused in time and that take advantage of the common mechanisms by which vessels normally adapt to stress.
Asunto(s)
Vasos Sanguíneos/fisiología , Sustancias de Crecimiento/fisiología , Animales , Endotelio Vascular/fisiología , Factores de Crecimiento de Fibroblastos/fisiología , Humanos , Óxido Nítrico/fisiología , Factor de Crecimiento Transformador beta/fisiologíaRESUMEN
In this review, the role of tyrosine kinases in angiotensin II-mediated signal transduction pathways in vascular smooth muscle is discussed. Angiotensin II was isolated by virtue of its vasoconstrictor abilities and has long been thought to play a critical role in hypertension. However, recent studies indicate important roles for angiotensin II in inflammation, atherosclerosis, and congestive heart failure. The expanding role of angiotensin II indicates that multiple signal transduction pathways are likely to be activated in a tissue-specific manner. Exciting recent data show that angiotensin II directly stimulates tyrosine kinases, including pp60(c-src) kinase (c-Src), focal adhesion kinase (FAK), and Janus kinases (JAK2 and TYK2). Angiotensin II may activate receptor tyrosine kinases, such as Axl and platelet-derived growth factor, by as-yet-undefined autocrine mechanisms. Finally, unknown tyrosine kinases may mediate tyrosine phosphorylation of Shc, Raf, and phospholipase C-gamma after angiotensin II stimulation. These angiotensin II-regulated tyrosine kinases appear to be required for angiotensin II effects, such as vasoconstriction, proto-oncogene expression, and protein synthesis, on the basis of studies with tyrosine kinase inhibitors. Thus, understanding angiotensin II-stimulated signaling events, especially those related to tyrosine kinase activity, may form the basis for the development of new therapies for cardiovascular diseases.
Asunto(s)
Angiotensina II/fisiología , Músculo Liso Vascular/citología , Proteínas Tirosina Quinasas/fisiología , Transducción de Señal/fisiología , Moléculas de Adhesión Celular/fisiología , Células Cultivadas , Quinasa 1 de Adhesión Focal , Proteína-Tirosina Quinasas de Adhesión Focal , Humanos , Desarrollo de Músculos , Músculo Liso Vascular/crecimiento & desarrollo , Fosforilación , Factor de Crecimiento Derivado de Plaquetas/fisiología , Proto-Oncogenes Mas , Familia-src Quinasas/fisiologíaRESUMEN
Integrin-mediated substrate adhesion of endothelial cells leads to intracellular signaling, including the activation of ERK 1/2 (extracellular regulated kinases 1 and 2), members of the mitogen-activated protein kinase (MAPK) family. MKP-1 is a dual-specificity protein phosphatase that may play an important role in regulating MAPK activity through dephosphorylation of threonine and tyrosine. Adhesion of human umbilical vein endothelial cells to fibronectin increased MKP-1 protein and mRNA levels, which reached a maximum at 60 min, while MAPK activity was maximal at 30 min. The MEK inhibitor PD98059 blocked activation of MAPK as well as the induction of MKP-1 during adhesion. The transcription inhibitor actinomycin D blocked MKP-1 induction and produced prolonged MAPK activation during adhesion. In contrast, endothelial adhesion to poly-L-lysine did not alter MAPK activity or MKP-1 levels. These findings demonstrate that integrin-mediated adhesion of endothelial cells to fibronectin results in transcriptional activation of MKP-1 through a MAPK-dependent mechanism. Regulation of MKP-1 by MAPK likely represents an important negative-feedback mechanism.
Asunto(s)
Adhesión Celular/fisiología , Proteínas de Ciclo Celular , Endotelio Vascular/citología , Endotelio Vascular/metabolismo , Fibronectinas/metabolismo , Proteínas Inmediatas-Precoces/metabolismo , Fosfoproteínas Fosfatasas , Proteínas Tirosina Fosfatasas/metabolismo , Células Cultivadas , Dactinomicina/farmacología , Fosfatasa 1 de Especificidad Dual , Endotelio Vascular/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Flavonoides/farmacología , Humanos , Proteínas Inmediatas-Precoces/genética , Integrinas/metabolismo , Quinasas de Proteína Quinasa Activadas por Mitógenos/antagonistas & inhibidores , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Proteína Fosfatasa 1 , Proteínas Tirosina Fosfatasas/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Transcripción Genética/efectos de los fármacosRESUMEN
Proliferation of smooth muscle cells in arteries is associated with contractile hypersensitivity to serotonin (5-HT). A possible explanation is that smooth muscle cells express increased numbers of phospholipase C (PLC)-coupled 5-HT receptors (5-HTR), which could mediate contractile and mitogenic signals via phosphatidylinositol turnover. To test this hypothesis, we performed a molecular characterization of 5-HTR subtypes in normal aorta and passaged rat aortic smooth muscle cells (RASM) in culture. Northern blot analysis revealed that growth-arrested cultured cells expressed 5-HT2R mRNA at 50-fold greater levels than aorta. 5-HT1CR mRNA was not detected in either case. 5-HT stimulated intracellular Ca2+ mobilization (fivefold peak increase) and c-fos mRNA induction (10-fold peak increase); both responses were strongly inhibited by selective 5-HT2R antagonists. Specific agonists for the 5-HT1AR, 5-HT1BR, and 5-HT1DR failed to induce c-fos mRNA. Although 5-HT (10 microM) increased [3H]thymidine incorporation (28% relative to 10% calf serum), it was a weak mitogen for cultured RASM based on cell counts. Thus there is high level expression of 5-HT2R mRNA by cultured RASM relative to aorta, and the 5-HT2R appears to be the only 5-HTR subtype mediating early growth signals in these cells. These data suggest that, following arterial injury in vivo, smooth muscle cells may overexpress the 5-HT2R, resulting in 5-HT contractile hypersensitivity and increased responsiveness to other growth factors.
Asunto(s)
Aorta/metabolismo , Músculo Liso Vascular/metabolismo , ARN Mensajero/metabolismo , Receptores de Serotonina/genética , Animales , Aorta/citología , Calcio/metabolismo , División Celular , Células Cultivadas , ADN/metabolismo , Masculino , Músculo Liso Vascular/citología , Proteínas Proto-Oncogénicas c-fos/metabolismo , Ratas , Ratas Endogámicas , Valores de Referencia , Serotonina/farmacologíaRESUMEN
Endothelial cells release nitric oxide (NO) acutely in response to increased "flow" or fluid shear stress (FSS), and the increase in NO production is correlated with enhanced phosphorylation and activation of endothelial nitric oxide synthase (eNOS). Both vascular endothelial growth factor and FSS activate endothelial protein kinase B (PKB) by way of incompletely understood pathway(s), and, in turn, PKB phosphorylates eNOS at Ser-1179, causing its activation. In this study, we found that either FSS or insulin stimulated insulin receptor substrate-1 (IRS-1) tyrosine and serine phosphorylation and increased IRS-1-associated phosphatidylinositol 3-kinase activity, phosphorylation of PKB Ser-473, phosphorylation of eNOS Ser-1179, and NO production. Brief pretreatment of bovine aortic endothelial cells with tumor necrosis factor-alpha (TNF-alpha) inhibited the above described FSS- or insulin-stimulated protein phosphorylation events and almost totally inhibited FSS- or insulin-stimulated NO production. These data indicate that FSS and insulin regulate eNOS phosphorylation and NO production by overlapping mechanisms. This study suggests one potential mechanism for the development of endothelial dysfunction in disease states with alterations in insulin regulation and increased TNF-alpha levels.
Asunto(s)
Factores de Crecimiento Endotelial/farmacología , Endotelio Vascular/fisiología , Insulina/farmacología , Linfocinas/farmacología , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico/metabolismo , Proteínas Serina-Treonina Quinasas , Proteínas Proto-Oncogénicas/metabolismo , Transducción de Señal/fisiología , Factor de Necrosis Tumoral alfa/farmacología , Animales , Aorta , Bovinos , Células Cultivadas , Endotelio Vascular/citología , Endotelio Vascular/efectos de los fármacos , Activación Enzimática , Proteínas Sustrato del Receptor de Insulina , Cinética , Óxido Nítrico Sintasa/efectos de los fármacos , Óxido Nítrico Sintasa de Tipo III , Fosfoproteínas/metabolismo , Fosforilación , Fosfoserina/metabolismo , Fosfotreonina/metabolismo , Fosfotirosina/metabolismo , Proteínas Proto-Oncogénicas c-akt , Transducción de Señal/efectos de los fármacos , Estrés Mecánico , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial VascularRESUMEN
Recent data suggest that uric acid is generated locally in the vessel wall by the action of xanthine oxidase. This enzyme, activated during ischemia/reperfusion by proteolytic conversion of xanthine dehydrogenase, catalyzes the oxidation of xanthine, thereby generating free radicals and uric acid. Because of the potential role of ischemia/reperfusion in vascular disease, we studied the effects of uric acid on rat aortic vascular smooth muscle cell (VSMC) growth. Uric acid stimulated VSMC DNA synthesis, as measured by [3H]thymidine incorporation, in a concentration-dependent manner with half-maximal activity at 150 microM. Maximal induction of DNA synthesis by uric acid (250 microM) was approximately 70% of 10% calf serum and equal to 10 ng/ml platelet-derived growth factor (PDGF) AB or 20 ng/ml fibroblast growth factor. Neither uric acid precursors (xanthine and hypoxanthine) nor antioxidants (ascorbic acid, glutathione, and alpha-tocopherol) were mitogenic for VSMC. Uric acid was mitogenic for VSMC but not for fibroblasts or renal epithelial cells. The time course for uric acid stimulation of VSMC growth was slower than serum, suggesting induction of an autocrine growth mechanism. Exposure of quiescent VSMC to uric acid stimulated accumulation of PDGF A-chain mRNA (greater than 5-fold at 8 h) and secretion of PDGF-like material in conditioned medium (greater than 10-fold at 24 h). Uric acid-induced [3H]thymidine incorporation was markedly inhibited by incubation with anti-PDGF A-chain polyclonal antibodies. Thus uric acid stimulates VSMC growth via an autocrine mechanism involving PDGF A-chain. These findings suggest that generation of uric acid during ischemia/reperfusion contributes to atherogenesis and intimal proliferation following arterial injury.
Asunto(s)
Músculo Liso Vascular/citología , Factor de Crecimiento Derivado de Plaquetas/biosíntesis , Ácido Úrico , Animales , Anticuerpos , Northern Blotting , División Celular , Células Cultivadas , ADN/biosíntesis , Cinética , Masculino , Músculo Liso Vascular/metabolismo , Factor de Crecimiento Derivado de Plaquetas/inmunología , Factor de Crecimiento Derivado de Plaquetas/metabolismo , Ratas , Ratas EndogámicasRESUMEN
Dental pathology and/or treatment have been linked to a small number of brain abscesses as possible sources of infection. A further case is presented, in which a dental site is implicated. A review of the evidence was undertaken. A wide range of dental procedures had been implicated. In some cases the brain isolate was not of dental origin. In many, the diagnosis was one of exclusion. In order to confirm the role of odontogenic infection in the pathogenesis of brain abscess, modern sampling techniques should be used to precisely identify the isolates. The causal organism should be identified in both oral and cranial sites.
Asunto(s)
Absceso Encefálico/etiología , Infección Focal Dental/complicaciones , Absceso Periodontal/complicaciones , Absceso Encefálico/diagnóstico por imagen , Absceso Encefálico/microbiología , Infección Focal Dental/microbiología , Hemiplejía/etiología , Humanos , Masculino , Persona de Mediana Edad , Absceso Periodontal/microbiología , Streptococcus sanguis/aislamiento & purificación , Tomografía Computarizada por Rayos XRESUMEN
Vasoconstrictors such as angiotensin II (ang II) stimulate vascular smooth muscle cell growth and share many signal transduction mechanisms with growth factors. Recently, growth factors have been shown to stimulate mitogen-activated protein (MAP) kinases, a family of serine/threonine protein kinases which phosphorylate pp90rsk, a cytosolic kinase that phosphorylates ribosomal S6 protein. We examined the effect of ang II on MAP kinase activity and phosphorylation. Ang II stimulated MAP kinase activity by 4-fold after 5 min exposure and also increased tyrosine phosphorylation of 42 kDa (74 +/- 41%) and 44 kDa (263 +/- 85%) proteins, shown to be pp42mapk and pp44mapk by Western blot analysis using a MAP kinase antibody. These results suggest that ang II-stimulated protein synthesis is mediated by a MAP kinase dependent pathway.
Asunto(s)
Angiotensina II/farmacología , Aorta/enzimología , Músculo Liso Vascular/enzimología , Fosfoproteínas/metabolismo , Proteínas Quinasas/metabolismo , Animales , Proteínas Quinasas Dependientes de Calcio-Calmodulina , Células Cultivadas , Electroforesis en Gel de Poliacrilamida , Activación Enzimática , Cinética , Masculino , Peso Molecular , Fosfoproteínas/aislamiento & purificación , Fosforilación , Fosfotirosina , Ratas , Ratas Sprague-Dawley , Tirosina/análogos & derivados , Tirosina/análisisRESUMEN
To study thrombin's receptor-mediated effects on vascular cells, we cloned and characterized a cDNA encoding a rat smooth muscle cell thrombin receptor. A rat aortic smooth muscle (RASM) cell cDNA library was screened with a 500-base pair (bp) sequence from the human thrombin receptor, obtained by polymerase chain reaction (PCR) amplification of cDNA synthesized from human erythropoietic leukemia (HEL) cell mRNA with PCR primers based on the published human thrombin receptor sequence. Clone pRTHR17 contains a 3418-bp insert that includes 50 bp of the 5'-untranslated region and the entire coding and 3'-untranslated regions of the RASM cell thrombin receptor. The sequence of pRTHR17 is 85% similar, at the nucleotide level, and 78% similar, at the deduced amino acid level, to the human thrombin receptor. Although the putative thrombin cleavage and binding sites are present, there are significant differences between the rat and human receptors in their amino-terminal sequences. Detectable signals (consisting of a single band of 3.45 kb) are present by Northern analysis of mRNA from RASM cells, and rat lung, kidney, and testes, but not in aorta or other tissues probed. The results of Southern analysis of rat genomic DNA are consistent with the existence of a single copy of the gene encoding this receptor. The steady state thrombin receptor mRNA level is low in cultured growth-arrested RASM cells and not detectable in rat aorta. To determine whether regulation of the RASM cell thrombin receptor occurs under growth-stimulating conditions, growth-arrested RASM cells were treated with basic fibroblast growth factor (bFGF, recently proposed to be a major mitogen controlling vascular smooth muscle cell growth following injury (Lindner, V., and Reidy, M. A. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 3739-3743)). There was a significant increase in thrombin receptor mRNA following the addition of bFGF. These data demonstrate that: 1) mRNA for a thrombin receptor similar to that reported from human megakaryocyte and hamster fibroblast cell lines is present in proliferating primary culture rat smooth muscle cells, 2) the most significant sequence differences are present in the amino-terminal tail of the thrombin receptor, and 3) the mRNA level for this receptor is regulated under growth-stimulating conditions in vitro.
Asunto(s)
Factor 2 de Crecimiento de Fibroblastos/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Músculo Liso Vascular/fisiología , Receptores de Superficie Celular/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Cricetinae , Biblioteca de Genes , Humanos , Leucemia Eritroblástica Aguda/genética , Masculino , Datos de Secuencia Molecular , Especificidad de Órganos , Reacción en Cadena de la Polimerasa/métodos , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Receptores de Trombina , Mapeo Restrictivo , Homología de Secuencia de Ácido Nucleico , Trombina/metabolismoRESUMEN
At near-threshold substance P concentrations, the isometric tension response of bovine tracheal strips is almost completely abolished by atropine, indicating mediation of contraction via substance P-stimulated release of acetylcholine from prejunctional nerve terminals. At near-maximal concentrations, the atropine-inhibited component of the tension response is less than 25%, indicating mainly direct activation. Under conditions in which activation by substance P is direct, peak tension is reached in approximately 11 min. Immunoblot analysis of the time course of phosphorylation of the 20-kDa myosin light chain (LC20) reveals incorporation of approximately 0.5 mol phosphate/mol light chain at 10 min. Two-dimensional tryptic phosphopeptide analysis of phosphorylated light chain reveals a single major phosphopeptide. The peptide migrates identically with that produced by myosin light chain kinase phosphorylation of purified tracheal myosin in vitro. Contraction stimulated by acetylcholine is more rapid, with attainment of peak tension in 2.5 min and a peak LC20 phosphorylation of 0.65 mol/mol. These results indicate that 1) substance P mediates contraction of bovine trachea both directly and indirectly, and 2) under conditions in which activation is direct, the tension and phosphorylation responses qualitatively resemble those observed with acetylcholine.
Asunto(s)
Contracción Muscular/efectos de los fármacos , Músculo Liso/fisiología , Quinasa de Cadena Ligera de Miosina/metabolismo , Sustancia P/farmacología , Tráquea/fisiología , Animales , Atropina/farmacología , Calcio/farmacología , Bovinos , Activación Enzimática , Glicopéptidos/farmacología , Técnicas In Vitro , Indometacina/farmacología , Ketanserina/farmacología , Cinética , Masoprocol/farmacología , Músculo Liso/efectos de los fármacos , Músculo Liso/enzimología , Subfragmentos de Miosina/aislamiento & purificación , Subfragmentos de Miosina/metabolismo , Mapeo Peptídico , Fosforilación , Cloruro de Potasio/farmacología , Prazosina/farmacología , Propranolol/farmacología , Pirilamina/farmacología , Tráquea/efectos de los fármacos , Tráquea/enzimologíaRESUMEN
Fluid shear stress is one of the most important mechanical forces acting upon vascular endothelium, because of its location at the interface between the bloodstream and vascular wall. Recent evidence indicates that several intracellular signaling events are stimulated in endothelial cells in response to shear stress. Through these events, shear stress modulates endothelial cell function and vascular structure, but the molecular basis of shear stress mechanotransduction remains to be elucidated. In our research we have focused on three temporal signal responses to shear stress: (1) production of nitric oxide (NO) as an immediate response; (2) activation of extracellular-regulated kinases (ERK1/2; p44/p42 mitogen-activated protein (MAP) kinases) as a rapid response, and (3) tyrosine phosphorylation of focal adhesion kinase (FAK) as a sustained response. In terms of vessel biology, NO production, and ERK1/2 and FAK activation seem to be correlated with vascular homeostasis, gene expression and cytoskeletal rearrangement, respectively. In this review, we discuss the mechanisms that establish the temporal order of shear stress-stimulated responses based on a hierarchy for assembly of signal transduction molecules at the cell plasma membrane.
Asunto(s)
Endotelio Vascular/fisiología , Proteínas Quinasas Activadas por Mitógenos , Transducción de Señal , Animales , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Moléculas de Adhesión Celular/metabolismo , Endotelio Vascular/citología , Quinasa 1 de Adhesión Focal , Proteína-Tirosina Quinasas de Adhesión Focal , Humanos , Proteína Quinasa 1 Activada por Mitógenos , Proteína Quinasa 3 Activada por Mitógenos , Óxido Nítrico/biosíntesis , Fosforilación , Proteínas Tirosina Quinasas/metabolismo , Estrés Mecánico , Tirosina/metabolismoRESUMEN
BACKGROUND: This case report describes a rare benign tumour, which presented as discrete areas of gingival hyperplasia affecting both the mandible and the maxilla. METHOD: Surgical excision of the lesions was carried out under local anaesthetic. Histopathological examination confirmed the diagnosis of oral myofibromatosis. RESULTS: The condition responded to surgical excision and appears to have limited growth potential. It affects a wide spectrum of ages and can be alarming due to rapid enlargement and ulceration, so careful diagnosis is important to avoid unnecessary aggressive treatment.
Asunto(s)
Hiperplasia Gingival/etiología , Neoplasias Gingivales/complicaciones , Miofibromatosis/complicaciones , Actinas/análisis , Adolescente , Diagnóstico Diferencial , Fibroblastos/patología , Neoplasias Gingivales/patología , Humanos , Masculino , Músculo Liso/patología , Miofibromatosis/patologíaRESUMEN
Endothelial nitric oxide synthase (eNOS) has been shown to be regulated both transcriptionally and posttranslationally in cultured endothelial cells, but eNOS regulatory mechanisms in vivo have not been elucidated. Because one of the strongest stimuli for eNOS expression in tissue culture is cell proliferation and because increased NO production would be beneficial in the setting of arterial injury, we hypothesized that eNOS expression should be increased in regenerating endothelium after a denuding injury. Rat aortas underwent partial endothelial denudation by passage of a deflated balloon catheter, and eNOS expression was studied 48 hours after injury. Immunohistochemistry with eNOS monoclonal antibody, NADPH diaphorase activity assay under conditions specific for eNOS, and mRNA hybridization were performed in situ on perfusion-fixed rat aortic segments. The vessels were studied en face to enhance visualization compared with cross sections. eNOS protein and mRNA expression were significantly increased in regenerating and migrating endothelial cells at the wound edge, with translocation of eNOS to the plasma membrane at the leading edge. Similar results were obtained when endothelial cells were studied in a tissue culture wound model. An important role for transforming growth factor (TGF)-beta1 in regulating eNOS expression was suggested by the ability of a TGF-beta1-neutralizing antibody to limit induction of eNOS at the wound edge. Increased eNOS expression after wounding appears to be related to signal events associated with cell migration as well as proliferation, because eNOS expression in vivo increased in nonproliferating cells and TGF-beta1-neutralizing antibody inhibited eNOS expression but stimulated proliferation. The current study is the first to suggest an important role in vivo for increased eNOS, and perhaps NO production, in the process of endothelial regeneration and wound repair.