Biomonitoring of children and adolescents using orthodontic appliances made of acrylic resins through micronucleus testing of exfoliated buccal and palatal mucosa cells.

INTRODUCTION: The present study aimed to investigate the occurrence of micronuclei and the degenerative nuclear alterations indicative of apoptosis and necrosis in the exfoliated buccal and palatal mucosa cells of children and adolescents using orthodontic appliances made of self-curing acrylic resins. METHODS: The micronuclei and nuclear alterations were evaluated in a minimum of 2000 cells collected from the cheeks and palates of 30 patients of both sexes, aged between 6 and 12 years. The cell evaluations were performed before appliance installation and 15 to 21 days after installation. A conditional test was used. In all analyses, the significance level was 5%. RESULTS: No differences in the occurrence of micronuclei or the nuclear degenerative changes indicative of apoptosis and necrosis were observed in the cheek cells between the 2 time points (P > 0.05). However, evaluation of the palate cells revealed a significant increase in the occurrence of micronuclei and the nuclear alterations indicative of apoptosis (P < 0.01) but not of karyolysis (P > 0.05). CONCLUSIONS: Direct contact of orthodontic appliances made of acrylic resins with the oral mucosa increases the incidence of chromosomal damage and degenerative nuclear alterations.

Phonetic damage related to unilateral functional inhibition of the lingual nerve.

PURPOSE: The aim of this study was to analyze the effects of unilateral functional inhibition of the lingual nerve in phonetic function by perceptual and acoustic analyses of speech, which was recorded before and after neurosensory modification of the lingual nerve, and a comparison of results of these evaluations with a subject's self-perception. PATIENTS AND METHODS: The sample consisted of 10 volunteers who had the lingual nerve of the right side anesthetized. The analyzed recordings consisted of terms directly dependent on the tongue for production. In the perceptual analysis, pitch and loudness behaviors were observed, in addition to coarticulation and intelligibility. The acoustic analysis of 5 vowels and sibilant /s/ was realized. A questionnaire about the discomfort level in speech production was applied. RESULTS: Perceptual/auditive and acoustic parameters showed no significant differences. Although 3 informants declared some kind of discomfort related to speech production, they presented no acoustic or perceptual modification in sound quality. CONCLUSIONS: Although inhibition of sensory function of the lingual nerve generated considerable annoyance, there was no specific damage to phonetic function in the group under study, because no differences were observed in the quality of the speech of subjects after applying anesthesia to the lingual nerve.

Uso da radiografia panorâmica como fonte de informação para a identificação humana ­ relato de caso pericial / Use of panoramic radiograph as a source for human identification ­ forensic case report

Introdução: A odontologia legal permite a identificação humana por meio da comparação de dados observados em uma documentação odontológica ante mortem (AM) com as informações coletadas post mortem (PM), sendo os exames radiográficos grandes aliados neste processo. Objetivo: Demostrar a contribuição da radiografia panorâmica como fonte de informação para a identificação humana. Relato do caso: foi encaminhado um corpo carbonizado, com parte da região bucomaxilofacial preservada e que ao exame necroscópico era possível identificar a presença de restaurações e ausências dentais. A apresentação de radiografia panorâmica anterior à morte e a realização de exame radiográfico panorâmico no corpo carbonizado possibilitou a comparação de pontos coincidentes e divergências explicáveis, permitindo a identificação positiva do caso. Conclusão: Ao final da perícia foi determinada a identidade da vítima e foi comprovada a importância da radiografia panorâmica para a identificação humana com base em caracteres identificadores anatômicos e terapêuticos presentes no complexo bucomaxilofacial

Introduction: Forensic dentistry allows human identification through the comparison of data observed in ante-mortem (AM) dental documentation with information collected post-mortem (PM), with radiographic examinations being great allies in this process. Objective: To demonstrate the contribution of panoramic radiography as a source of information for human identification. Case report: a charred body was sent, with part of the oral and maxillofacial region preserved and upon necroscopic examination it was possible to identify the presence of restorations and missing teeth. The presentation of a panoramic radiograph prior to death and the performance of a panoramic radiographic examination of the charred body made it possible to compare coincident points and explainable divergences, allowing positive identification of the case. Conclusion: At the end of the forensic examination of the case, the identity of the victim was determined and the importance of panoramic radiography for human identification based on anatomical and therapeutic identifying characters present in the oral and maxillofacial complex was proven

Alterações dimensionais em marcas de mordidas humanas simuladas em alimentos: estudo in vitro / Dimensional changes in simulated human bite marks on food: in vitro study

Objetivo: avaliar alterações dimensionais em marcas de mordidas simuladas em um tipo de queijo e de goiabada com o decorrer do tempo. Materiais e Métodos: mordidas foram simuladas em 20 amostras-padrão formato retangular nas medidas 30 x 60 mm e espessura de 20 mm de queijo tipo muçarela (n=10) e goiabada (n=10) com a utilização de um manequim odontológico montado em oclusão normal e acoplado à máquina universal de ensaios mecânicos. Amostras foram mantidas em superfície de vidro em ambiente com temperatura e umidade controladas seguida da aferição das distâncias intercaninas das marcas a cada hora com auxílio de paquímetro digital, durante 120 horas de experimentação. A temperatura e umidade mantiveram-se constantes durante todo período. As medidas obtidas foram estatisticamente analisadas por meio dos testes de Friedman, de Wilcoxon, coeficiente de correlação de Spearman e a partir da análise de regressão linear simples. O nível de significância adotado foi de 5%. Resultados: os alimentos testados mantiveram o padrão das medidas lineares nas primeiras quatro horas (p>0,05). Após esse período, ocorreu uma deformação progressiva, levando ao aumento significativo das medidas (p<0,05). Conclusão: a marca de mordida em queijo tipo muçarela e goiabada apresentou-se dimensionalmente estável nas primeiras quatro horas, pós mordida. Após esse período, as marcas já não apresentaram características favoráveis para serem utilizadas como registro para fins de comparação.

Objective: to evaluate dimensional changes in simulated bite marks in a type of cheese and guava paste over time. Materials and Methods: Bites were simulated on 20 standard rectangular samples. in the measures 30 x 60 mm and thickness of 20 mm of mozzarella cheese (n=10) and guava paste (n=10) using a dental manikin mounted on normal occlusion and coupled to the universal mechanical testing machine. The samples were kept on a glass surface in an environment with controlled temperature and humidity , followed by the measurement of intercanine distances of the marks, every hour, with the aid of a caliper digital, during 120 hours of experimentation. The temperature and humidity remained constant throughout the period. The measurements obtained were statistically analyzed using the Friedman, Wilcoxon, Spearman's correlation coefficient and from the linear regression analysis simple (IBM SPSS. 21.0, 2012, Armonk, NY: IBM Corp.). The level of significance adopted was 5%. Results: the foods tested maintained the pattern of linear measurements in the first four hours (p>0.05). After this period, a progressive deformation occurred, leading to the significant increase in measurements (p<0.05). Conclusion: the bite mark in mozzarella cheese and guava paste it was dimensionally stable in the first four hours, post-bite. After this period, brands no longer have favorable characteristics to be used as a record for comparison purposes.

Evaluation of the Genotoxicity of Endodontic Materials for Deciduous Teeth Using the Comet Assay

ABSTRACT Objective: To evaluate genotoxicity of zinc oxide, P. A. calcium hydroxide, mineral trioxide aggregate and an iodoform paste using comet assay on human lymphocytes. Material and Methods: Two positive controls were used: methyl-methanesulfonate for the P.A. calcium hydroxide and mineral trioxide aggregate; and doxorubicin for the iodoform paste and zinc oxide. There were also two negative controls: distilled water for the P.A. calcium hydroxide and mineral trioxide aggregate; and DMSO for the iodoform paste and zinc oxide. Comets were identified using fluorescence microscopy and 100 of them were counted on each of the three slides analyzed per drug test. A damage index was established, taking into consideration the score pattern that had previously been determined from the size and intensity of the comet tail. Analysis of variance, followed by Tukey's test, was used to compare the means of the DNA damage indices. Results: The DNA damage index observed for mineral trioxide aggregate (7.08 to 8.58) and P.A. calcium hydroxide (6.50 to 8.33), which were similar to negative control index. On the other hand, damage index for zinc oxide (104.7 to 218.50) and iodoform paste (115.7 to 210.7) were similar to positive control index. Conclusion: Iodoform paste and zinc oxide showed genotoxicity at all concentrations used.

Does the facial pattern give individuals a profile of a crime suspect? / O padrão facial confere aos indivíduos o perfil suspeito a cometer um crime?

To evaluate the influence of mesofacial, brachyfacial and dolichofacial facial patterns on giving an individual the profile of a crime suspect in the eyes of public security agents.This study had a cross-sectional design, conducted with public security agents of both sexes (n=100), where images of facial composites (police sketches) of individuals with different facial patterns (mesofacial, brachyfacial and dolichofacial) were used. With these images in hand, a questionnaire was created, divided into three parts: the first in which all the images were presented together, allowing comparison among them; the second, in which each image was evaluated separately followed by questions and the third that consisted on a visual analog scale that presented a bar with marks going from 0 to 100, where 0 represented the untrustworthy individual, 50 the individual who could be trusted, and 100 a very trustworthy individual. When all the data had been obtained statistical analyses were performed using the Chi-square and Friedman tests. The level of significance adopted was 5% (α=0.05). The dolichofacial individual was associated with security agents as the most prone to commit crimes and became more insecure and distrustful when compared to the mesofacial and brachyfacial individuals (p <0.001). The dolichofacial profile had a negative influence on the judgment of security agents who attributed to it, a character suspected of a crime and a low level of trustworthiness

Avaliar a influência dos padrões faciais mesofacial, braquifacial e dolicofacial em conceder um perfil de suspeito a cometer crimes aos olhos dos agentes de segurança pública. Este estudo teve delineamento transversal, realizado com agentes de segurança pública de ambos os sexos (n = 100), onde imagens de retratos- falados de indivíduos com diferentes padrões faciais (mesofacial, braquifacial e dolicofacial) foram utilizadas. De posse dessas imagens, foi criado um questionário, dividido em três partes: a primeira em que todas as imagens foram apresentadas juntas, permitindo a comparação entre elas; a segunda, em que cada imagem foi avaliada separadamente seguida de perguntas e a terceira consistiu em uma escala analógica visual que apresentava uma barra com marcações de 0 a 100, onde 0 representava o indivíduo não confiável, 50 o indivíduo em quem se podia confiar, e 100 um indivíduo muito confiável. Após a obtenção dos dados, foram realizadas análises estatísticas utilizando os testes Qui-quadrado e Friedman. O nível de significância adotado foi de 5% (α = 0,05). O indivíduo dolicofacial foi associado pelos agentes de segurança como o mais propenso a cometer crimes e passou mais insegurança e desconfiança, quando comparado aos indivíduos mesofacial e braquifacial (p<0,001). O perfil dolicofacial influenciou negativamente no julgamento dos agentes de segurança que atribuíram-lhe caráter suspeito a cometer crimes e baixo grau de confiabilidade.

Documentação ortodôntica digitalizada – ainda precisamos de papel? / Electronic orthodontic records – we still need papers?

Muitos dos processos de diagnóstico e planejamento no consultório de Ortodontia contemporâneo têm ocorrido de maneira digitalizada. Exames, anteriormente gerados apenas em suporte de papel, têm sido entregues ao profissional em formato digital e até enviados pela internet. Acompanhando essas transformações técnicas, muitos ortodontistas sonham em ter um consultório inteiramente informatizado, com todos os dados disponíveis em qualquer momento por acesso remoto. Com os avanços tecnológicos, a possibilidade da digitalização do prontuário físico, bem como do uso dos sistemas informatizados, se apresentam como excelentes opções para a manutenção da documentação odontológica. Além da evidente relevância clínica e administrativa, uma boa documentação é reconhecida como a melhor forma de defesa de um profissional quando sua conduta é colocada à prova. Neste contexto, é preciso que o ortodontista entenda os critérios técnicos e legais para incorporação dos meios digitais à sua rotina. O objetivo deste trabalho foi discutir as exigências legais da utilização de prontuários eletrônicos por especialistas em Ortodontia, bem como apresentar os aspectos práticos a serem considerados no caso da digitalização de documentos elaborados na clínica com o objetivo de armazenamento...

Many of the diagnostic and planning processes in contemporary orthodontic office has been digitized and many exams previously generated on paper has been delivered entirely digital and sent over the internet. Accompanying these technical changes, many orthodontists dream of having a fully digitized office, with all the information available at any time via remote access. With technological advances, the possibility of scanning physical records and the use of computerized systems, stand as excellent options for maintaining dental records. Apart from the obvious clinical and administrative relevance, a good documentation is recognized as the best form of defense of a professional when their conduct is questioned. In this context, it is necessary that the orthodontist understand the technical and legal criteria for incorporation of digital media to your routine. The objective of this paper is to discuss the legal requirements the use of electronic medical records by orthodontic specialists, as well as presenting practical aspects to be considered in the case of document scanning developed in the clinic with the purpose of storage...