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1.
Biochim Biophys Acta ; 1838(10): 2656-61, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24998361

RESUMEN

Polyphenols are well known as antioxidant agents and by their effects on the hydration layers of lipid interphases. Among them, gallic acid and its derivatives are able to decrease the dipole potential and to act in water as a strong antioxidant. In this work we have studied both effects on lipid interphases in monolayers and bilayers of dimyristoylphosphatidylcholine. The results show that gallic acid (GA) increases the negative surface charges of large unilamellar vesicles (LUVs) and decreases the dipole potential of the lipid interphase. As a result, positively charged radical species such as ABTS(+) are able to penetrate the membrane forming an association with GA. These results allow discussing the antiradical activity (ARA) of GA at the membrane phase which may be taking place in water spaces between the lipids.


Asunto(s)
Dimiristoilfosfatidilcolina/química , Depuradores de Radicales Libres/química , Ácido Gálico/química , Membrana Dobles de Lípidos/química
2.
J Phys Chem B ; 119(31): 10060-5, 2015 Aug 06.
Artículo en Inglés | MEDLINE | ID: mdl-26217964

RESUMEN

We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25 °C) and in liquid crystalline states (50 °C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does not produce significant changes in MC 540 monomer concentration in DPPC LUVs at 50 °C. In contrast, it significantly decreases the monomer adsorption in defects present in DPPC LUVs at 25 °C. When DPPC LUVs were subjected to hypertonic stress, dehydration caused more defects, and in this case phenylalanine is also able to block such defects.


Asunto(s)
Lípidos de la Membrana/química , Presión Osmótica , Fenilalanina/química , Liposomas Unilamelares/química , 1,2-Dipalmitoilfosfatidilcolina/química , Geles/química , Estructura Molecular
3.
J Phys Chem B ; 119(52): 15844-7, 2015 Dec 31.
Artículo en Inglés | MEDLINE | ID: mdl-26636175

RESUMEN

The negative free energy previously reported is explained by the stabilization of a PC-Phe (phosphocholine-phenylalanine) complex in the presence of water shown by the decrease in the symmetric stretching frequency of the phosphate group of the lipid (PO2(-)). An entropic contribution due to the disruption of the water network around the phenyl and in the membrane defect may be invoked. The dipole potential decrease is explained by the orientation of the carboxylate opposing to the CO of the lipids with oxygen moiety toward the low hydrated hydrocarbon core. The symmetric bending frequency of NH3(+) group of Phe, decreases in 5.2 cm(-1) in relation to water congruent with zeta potential shift to positive values. The Phe to DPPC dissociation constant is Kd = 2.23 ± 0.09 mM, from which the free energy change is about -4.54 kcal/mol at 25 °C. This may be due to hydrophobic contributions and two hydrogen bonds.


Asunto(s)
1,2-Dipalmitoilfosfatidilcolina/análogos & derivados , Fenilalanina/química , 1,2-Dipalmitoilfosfatidilcolina/química , Entropía , Enlace de Hidrógeno , Interacciones Hidrofóbicas e Hidrofílicas , Modelos Moleculares , Agua/química
4.
Colloids Surf B Biointerfaces ; 135: 504-509, 2015 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-26283499

RESUMEN

The influence of Phe on the surface pressure of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers at the air-water interface was studied at different initial surface pressures (26 and 40 mN/m) and two pHs (5.0 and 7.3) at constant temperature (20 °C). Changes produced by the aminoacid added to the subphase on the surface pressure and on the dipole potential of lipid monolayers were measured at a fixed area. Compressibility properties of the monolayers at different pHs were studied by (π-A) isotherms. The results suggest that Phe intercalates into a DPPC film at the air-water interface at pH 5 and forms a different arrangement at pH 7.3. The possible relevance of these results of the effect of Phe in physiological conditions is discussed.


Asunto(s)
Lípidos/química , Fenilalanina/química , 1,2-Dipalmitoilfosfatidilcolina/química , Algoritmos , Concentración de Iones de Hidrógeno , Membranas Artificiales , Propiedades de Superficie , Temperatura , Termodinámica
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