RESUMEN
We have previously shown that the LAZ3/BCL6 gene encoding a potential transcription factor, is disrupted in B-diffuse large cell non-Hodgkin's lymphomas (NHL) with 3q27 chromosomal abnormalities involving the immunoglobulin (IG) genes. However, LAZ3 rearrangement also occurs in NHL bearing 3q27 translocations without involvement of the IG genes: for example the VAl cell line exhibits t(3;4)(q27;p11). In the present work we have used a RT-PCR method to detect and to sequence the LAZ3 mRNA products from the VAL cell line. We report that the consequence of the t(3;4) is the expression of a chimeric transcript of LAZ3 with a new gene encoding a small G-like protein, termed TTF (Translocation Three Four). Nucleotide sequence analysis of a 1.4 kb cDNA predicts that the TTF gene encodes a protein of 191 amino-acids similar to members of the RAS superfamily including HRAS (27% identical), RAB1A (30% identical) and RHO proteins: the human RAC1, RHOB and CDC42Hs proteins (respectively 43, 44 and 45% identical) and the yeast RHO2 protein (44% identical). Unlike most other small G proteins which are expressed ubiquitously, TTF was transcribed only in hemopoietic cells as a 2.2 kb transcript. TTF may define a new subgroup of RHO-like proteins.
Asunto(s)
Clonación Molecular , Proteínas de Unión al ADN/genética , Proteínas de Unión al GTP/genética , Factores de Transcripción/genética , Translocación Genética , Secuencia de Aminoácidos , Secuencia de Bases , Cromosomas Humanos Par 3 , Cromosomas Humanos Par 4 , ADN Complementario , Humanos , Datos de Secuencia Molecular , Filogenia , Proteínas Proto-Oncogénicas c-bcl-6 , ARN Mensajero/genética , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Células Tumorales CultivadasRESUMEN
Isolation of homologous ras-related genes from sugar beet has never been reported. Screening cDNA library from Beta vulgaris L. Hilma resulted in the isolation of 3 ras-homologous clones. Two of these genes (rab1Bv and rab2Bv) belong to the Rab/Ypt group. The deduced polypeptidic sequences from them show strong homologies to Ara3 (93% with Rab1Bv) of Arabidopsis thaliana and Rgp2 (97% with Rab2Bv) of Oryza sativa. The third gene (rho1Bv) belongs to the Rho family. The homology of Rho1Bv protein with Rho1Ps from Pisum is very high (98%). Rho1Bv is the second representative plant Rho protein described in the literature. The homologies of all these 3 small GTP-binding proteins indicate that these proteins are conserved in plant families like Chenopodiaceae, Brassicaceae, Fabaceae and Poaceae and could control important transductional pathways conserved along the processes of evolution.