RESUMEN
The proposed phiKZ genus of myoviruses has 21 members. Phages are virulent, lyse Pseudomonas bacteria, and are characterized by very large heads and correspondingly high DNA contents. The genome of the type virus, phiKZ, has 306 ORFs and over 280 kbp and is the second-largest phage genome known. The phiKZ genus has very few relationships to other phages and includes three species and one possible species.
Asunto(s)
Myoviridae/clasificación , Myoviridae/genética , Myoviridae/patogenicidad , Fagos Pseudomonas/clasificación , Fagos Pseudomonas/genética , Fagos Pseudomonas/patogenicidad , Composición de Base , Secuencia de Bases , Mapeo Cromosómico , Cromosomas , ADN Circular , ADN Intergénico , ADN Viral/análisis , ADN Viral/aislamiento & purificación , Genoma Viral , Calor , Myoviridae/química , Myoviridae/aislamiento & purificación , Myoviridae/ultraestructura , Sistemas de Lectura Abierta , Fagos Pseudomonas/química , Fagos Pseudomonas/aislamiento & purificación , Fagos Pseudomonas/ultraestructura , ARN de Transferencia/genética , Proteínas Virales/análisis , Virión/química , Virión/ultraestructura , VirulenciaRESUMEN
BACKGROUND: Hepatitis C virus (HCV) is a major cause of hepatitis in hemodialysis (HD) patients. Routes other than blood transfusion play a role in the spread of HCV in HD patients. Molecular studies of HCV implicate nosocomial transmission of the virus in HD units. We conducted a clinicovirological study in our HD unit to investigate if the hands of dialysis personnel could represent a mode of transmission of HCV among HD patients. METHODS: One liter of sterile water was used for each handwashing of dialysis personnel. The washing was collected in a sterile container and tested for HCV-RNA by polymerase chain reaction (PCR) within 3 h of collection. Eighty handwashings from nurses dialyzing HCV-positive patients (groupe A) and 100 handwashing from nurses dialyzing HCV-negative patients (group B) were tested for HCV-RNA. As a control, 60 handwashings were collected from the dialysis personnel before entering the dialysis unit (group C) and tested for HCV-RNA. RESULTS: HCV-RNA was positive in 19 (23.75%) of samples of group A, in 8 (8%) of samples of group B (p < 0.003) and in 2 (3.3%) of samples of group C (p < 0. 35). These two positive samples of group C were from nurses who had dialyzed HCV-negative patients. CONCLUSION: These results indicate the presence of HCV-RNA on the hands of some dialysis personnel in our HD unit, in spite fo adherence to the standard precautions. The hands of dialysis personnel are therefore a potential mode for facilitating transmission of HCV between HD patients.
Asunto(s)
Mano/virología , Hepacivirus/aislamiento & purificación , Hepatitis C/transmisión , Transmisión de Enfermedad Infecciosa de Paciente a Profesional , Enfermeras Practicantes , Diálisis Renal , Infección Hospitalaria/prevención & control , Infección Hospitalaria/transmisión , Infección Hospitalaria/virología , Cartilla de ADN/química , Desinfección de las Manos , Unidades de Hemodiálisis en Hospital , Hepacivirus/genética , Hepatitis C/virología , Anticuerpos contra la Hepatitis C/análisis , Humanos , ARN Viral/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
[35S]Methionine-labelled envelope polypeptides of herpes simplex virus type 1, strain F, propagated in mammalian cell culture of various origins, were separated by ion-exchange high-performance liquid chromatography on a TSK DEAE-3SW column. Analysis of the fractions by radioimmunoprecipitation followed by sodium dodecyl sulphate polyacrylamide gel electrophoresis of the immunoprecipitates showed similarities as well as distinct differences in the number, migration patterns and molecular mass of the synthesized polypeptides, depending on the host cell. The results show that this method can be used to demonstrate species-specific or organ-specific differences in the processing of virus-specified polypeptides synthesized in host cells.