RESUMEN
The authors studied the ability for anaphylactic contraction in vitro of musculus plantaris, diaphragmatic muscle and musculus soleus of guinea pig. The muscles of double sensitized pigs were denervated and 4 weeks after the nerve cut the muscles' specimens were studied for anaphylactic contraction. The addition of the resolving dose of the specific antigen to incubation medium resulted in anaphylactic contraction of the diaphragmatic muscle and musculus soleus. Musculus plantaris did not respond. Mechanisms of anaphylactic contraction of the skeletal muscle are discussed.
Asunto(s)
Anafilaxia/fisiopatología , Contracción Muscular/fisiología , Músculo Esquelético/fisiopatología , Anafilaxia/etiología , Anafilaxia/metabolismo , Animales , Cobayas , Inmunización/métodos , Inmunohistoquímica , Técnicas In Vitro , Masculino , Desnervación Muscular/métodos , Fibras Musculares Esqueléticas/metabolismo , Músculo Esquelético/metabolismo , Anafilaxis Cutánea Pasiva , FenotipoRESUMEN
The immunohistochemical profile of intact and denervated soleus muscle of guinea pigs after sensibilization was studied. It is shown, that intact soleus muscle consists of slow fibers, which have low ATP-ase activity and don't react with monoclonal antibodies against fast myosin heavy chain. No changes of immunohistochemical profile were found after denervation or sensibilization. At the same time, the fibers, reacting with monoclonal antibodies against fast myosin heavy chain and having low ATP-ase activity, were found in denervated muscles after sensibilization. It is concluded, that the synthesis of fast myosin is induced after sensibilization of denervated muscles. Validity of myosin ATP-ase histochemistry for muscle fibers typing is discussed.
Asunto(s)
Músculos/metabolismo , Miosinas/biosíntesis , Animales , Anticuerpos Monoclonales/administración & dosificación , Desnervación , Cobayas , Inmunohistoquímica , Masculino , Músculos/inervación , Miosinas/metabolismoRESUMEN
The influence of the serotin biological activity on forming the "shock" lung syndrome was revealed in experiments on rats. Tachyhyperpnea with predominance of functional emphysema and a small number of atelectic tissue areas were observed in the animals with traumatic "shock" during the serotonin hypersecretion. Tachyhypopnea with a significant predominance of atelectiv areas was seen during the serotonin hypoproduction.