RESUMEN
OBJECTIVE: We studied the relationship between uninstructed, unstructured movements and neural activity in three epilepsy patients with intracranial electroencephalographic (iEEG) recordings. APPROACH: We used a custom system to continuously record high definition video precisely time-aligned to clinical iEEG data. From these video recordings, movement periods were annotated via semi-automatic tracking based on dense optical flow. MAIN RESULTS: We found that neural signal features (8-32 Hz and 76-100 Hz power) previously identified from task-based experiments are also modulated before and during a variety of movement behaviors. These movement behaviors are coarsely labeled by time period and movement side (e.g. 'Idle' and 'Move', 'Right' and 'Left'); movements within a label can include a wide variety of uninstructed behaviors. A rigorous nested cross-validation framework was used to classify both movement onset and lateralization with statistical significance for all subjects. SIGNIFICANCE: We demonstrate an evaluation framework to study neural activity related to natural movements not evoked by a task, annotated over hours of video. This work further establishes the feasibility to study neural correlates of unstructured behavior through continuous recording in the epilepsy monitoring unit. The insights gained from such studies may advance our understanding of how the brain naturally controls movement, which may inform the development of more robust and generalizable brain-computer interfaces.
Asunto(s)
Encéfalo/fisiología , Electrocorticografía/métodos , Epilepsia/fisiopatología , Movimiento/fisiología , Grabación en Video/métodos , Adolescente , Epilepsia/diagnóstico , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Substance P (SP) is known to be a peptide that facilitates epileptic activity of principal cells in the hippocampus. Paradoxically, in other models, it was found to be protective against seizures by activating substance P receptor (SPR)-expressing interneurons. Thus, these cells appear to play an important role in the generation and regulation of epileptic seizures. The number, distribution, morphological features and input characteristics of SPR-immunoreactive cells were analyzed in surgically removed hippocampi of 28 temporal lobe epileptic patients and eight control hippocampi in order to examine their changes in epileptic tissues. SPR is expressed in a subset of inhibitory cells in the control human hippocampus, they are multipolar interneurons with smooth dendrites, present in all hippocampal subfields. This cell population is considerably different from SPR-positive cells of the rat hippocampus. The CA1 (cornu Ammonis subfield 1) region was chosen for the detailed morphological analysis of the SPR-immunoreactive cells because of its extreme vulnerability in epilepsy. The presence of various neurochemical markers identifies functionally distinct interneuron types, such as those responsible for perisomatic, dendritic or interneuron-selective inhibition. We found considerable colocalization of SPR with calbindin but not with parvalbumin, calretinin, cholecystokinin and somatostatin, therefore we suppose that SPR-positive cells participate mainly in dendritic inhibition. In the non-sclerotic CA1 region they are mainly preserved, whereas their number is decreased in the sclerotic cases. In the epileptic samples their morphology is considerably altered, they possessed more dendritic branches, which often became beaded. Analyses of synaptic coverage revealed that the ratio of symmetric synaptic input of SPR-immunoreactive cells has increased in epileptic samples. Our results suggest that SPR-positive cells are preserved while principal cells are present in the CA1 region, but show reactive changes in epilepsy including intense branching and growth of their dendritic arborization.
Asunto(s)
Epilepsia/patología , Hipocampo/patología , Interneuronas/metabolismo , Interneuronas/patología , Sustancia P/metabolismo , Sinapsis/patología , Adulto , Anciano , Recuento de Células/métodos , Dendritas/metabolismo , Dendritas/ultraestructura , Femenino , Humanos , Inmunohistoquímica/métodos , Interneuronas/clasificación , Interneuronas/ultraestructura , Masculino , Microscopía Inmunoelectrónica/métodos , Persona de Mediana Edad , Proteínas del Tejido Nervioso/metabolismo , Cambios Post Mortem , Sinapsis/clasificación , Sinapsis/metabolismo , Sinapsis/ultraestructuraRESUMEN
It is known that epilepsy patients diagnosed with neocortical temporal lobe epilepsy (NTLE), differ from those diagnosed with mesial temporal lobe epilepsy (MTLE), e.g., in hippocampal (HPC) pathology. In the present studies, we tested the hypothesis that NTLE and MTLE subtypes of human epilepsy might differ in regards to their HPC monoamine neurochemistry. Monoamine neurotransmitters were studied in separate signals and within s with semiderivative microvoltammetry, used in combination with stearate indicator, Ag-AgCl reference and stainless steel auxiliary microelectrodes. Anterior HPC specimens from the patients' epileptogenic zone, defined by electrocorticography, were resected neurosurgically from 13 consecutive patients with intractable temporal lobe epilepsy. Four patients were diagnosed with NTLE and nine with MTLE. The criteria for the diagnosis of NTLE versus MTLE was absence versus presence of HPC sclerosis, respectively, based on MRI examination of resected tissue. In addition, NTLE patients demonstrated seizure onset in anterolateral temporal neocortex on electroencephalography (EEG). HPC subparcellations studied were: (a) Granular Cells of the Dentate Gyrus (DG), (b) Polymorphic Layer of DG and (c) Pyramidal Layer: subfields, CA1 and CA2. Dopamine (DA), serotonin (5-HT), norepinephrine (NE) and ascorbic acid (AA) (co-factor in DA to NE synthesis), exhibited separate and characteristic half-wave potentials in millivolts. Each half-wave potential, i.e., the potential at which maximum current was generated, was experimentally established in vitro. Concentrations of neurotransmitters found in HPC subparcellations were interpolated from calibration curves derived in vitro from electrochemical detection of monoamines and AA in saline phosphate buffer. Significant differences between subtypes in concentration of monoamines were analyzed by the Mann Whitney rank sum test and those differences in probability distribution of monoamines were analyzed by the Fisher Exact test; in each case, P<0.01 was the criteria selected for determining statistical significance. DA concentrations were higher in NTLE compared with MTLE in each HPC subparcellation [P=0.037, 0.024 and 0.007, respectively (P<0.01)] and DA occurred more frequently in NTLE in the Pyramidal Layer [P=0.077 (P<0.01)]. AA was present in one NTLE patient. NE concentrations were higher in MTLE vs. NTLE in each subparcellation [P=0.012, 0.067 and 0.07, respectively (P<0.01)] and NE occurred more frequently in MTLE in Granular Cells of DG and Pyramidal Layer [P=0.052 and 0.014, respectively (P<0.01)]. In MTLE, NE concentrations in the CA1 subfield of the Pyramidal Layer were decreased vs. the CA2 subfield [P=0.063 (P<0.01)]. Serotonin was found in every HPC subparcellation of each subtype but 5-HT concentrations were higher in NTLE vs. MTLE in the Granular Cells of DG and the Pyramidal Layer (CA1 subfield) [P=0.076 and 0.095, respectively (P<0.01)]. Thus, this preliminary study showed that marked differences in HPC monoamine neurochemistry occurred in NTLE patients as compared with MTLE patients.
Asunto(s)
Monoaminas Biogénicas/metabolismo , Epilepsia del Lóbulo Temporal/fisiopatología , Hipocampo/metabolismo , Neocórtex/fisiopatología , Neurotransmisores/metabolismo , Lóbulo Temporal/fisiopatología , Adulto , Ácido Ascórbico/metabolismo , Giro Dentado/metabolismo , Giro Dentado/patología , Dopamina/metabolismo , Electrofisiología/métodos , Epilepsia del Lóbulo Temporal/metabolismo , Epilepsia del Lóbulo Temporal/patología , Femenino , Humanos , Masculino , Norepinefrina/metabolismo , Células Piramidales/metabolismo , Serotonina/metabolismo , Distribución TisularRESUMEN
Biogenic amines in well defined subtypes of human temporal lobe epilepsy (TLE) have not been well characterized. Specimens from five patients with neocortical TLE (NTLE) and nine with mesial TLE (MTLE) were immediately placed in Ringer's lactate; stearate indicator microelectrodes were placed in temporal gray matter, Ag/AgCl reference microelectrodes and auxiliary microelectrodes were placed 3-7 mm contralaterally to the indicator microelectrode. Dopamine (DA), ascorbic acid (AA), norepinephrine (NE) and serotonin (5-HT) were identified by their characteristic oxidative potentials in vitro. Four of five patients with NTLE had NE depletion in temporal neocortex while eight of nine patients with MTLE had high concentrations of NE (chi-square P<0.01). Significant concentrations of DA were present in the temporal lobes of three of five NTLE patients but in only one of the nine MTLE patients (chi-square P<0.05). 5-HT was present in the neocortex of both NTLE and MTLE patients in similar concentrations. AA was found in the neocortex of one NTLE patient. These data support an association between NE depletion and NTLE. The relative NE deficiency along with the consistent presence of DA in NTLE patients suggest an impairment in the catecholamine pathway. The presence of AA, a co-factor in NE synthesis, in the neocortex of one NTLE patient may also be related since AA is a cofactor in NE synthesis.
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Aminas Biogénicas/metabolismo , Epilepsia del Lóbulo Temporal/metabolismo , Neocórtex/metabolismo , Distribución de Chi-Cuadrado , Electroquímica/instrumentación , Electroquímica/estadística & datos numéricos , Epilepsia del Lóbulo Temporal/patología , Epilepsia del Lóbulo Temporal/cirugía , Humanos , Microelectrodos , Neocórtex/patología , Neocórtex/cirugía , Estadísticas no ParamétricasRESUMEN
Pediatric meningiomas are uncommon. Those presenting in the third ventricle are rare; there are only 15 cases reported in the literature. We report an additional third ventricular meningioma in a 6-year-old boy. The tumor was resected via an anterior transcallosal interfornicial approach. Postoperatively, the patient exhibited a transient episode of mutism. Unlike previous reports, the pathological diagnosis of this lesion was an atypical meningioma. The presentation, management, pathological features, and postoperative course of our case are discussed and compared with previous reports.
Asunto(s)
Neoplasias del Ventrículo Cerebral/cirugía , Neoplasias Meníngeas/cirugía , Meningioma/cirugía , Neoplasias del Ventrículo Cerebral/patología , Ventrículos Cerebrales/patología , Niño , Humanos , Masculino , Neoplasias Meníngeas/patología , Meningioma/patologíaRESUMEN
OBJECTIVE: Indwelling intrathecal drug delivery systems are becoming increasingly important as a method of neuromodulation within the nervous system. In particular, intrathecal baclofen therapy has shown efficacy and safety in the management of spasticity and dystonia in children. The most common complications leading to explantation of the pumps are skin breakdown and infection at the pump implantation site. The pediatric population poses particular challenges with regard to these complications because appropriate candidates for intrathecal baclofen therapy are often undernourished and thus have a dearth of soft tissue mass to cover a subcutaneously implanted baclofen pump. We report a technique of subfascial implantation that provides greater soft tissue coverage of the pump, thereby reducing the potential for skin breakdown and improving the cosmetic appearance of the implantation site. METHODS: Eighteen consecutively treated children (average age, 8 yr, 7 mo) with spasticity and/or dystonia underwent subfascial implantation of a baclofen pump. These children's mean weight of 42.9 lb is less than the expected weight for a group of children in this age group, ranging from 4 years, 8 months, to 15 years, 7 months. In all patients, the pump was inserted into a pocket surgically constructed between the rectus abdominus and the external oblique muscles and the respective anterior fascial layers. RESULTS: At an average follow-up of 13.7 months, no infection or skin breakdown had occurred at the pump surgical site in any of the 18 patients. CONCLUSION: At this early follow-up, the subfascial implantation technique was associated with a reduced rate of local wound and pump infections and provided optimal cosmetic results as compared with that observed in retrospective cases.
Asunto(s)
Baclofeno/uso terapéutico , Distonía/tratamiento farmacológico , Bombas de Infusión Implantables , Relajantes Musculares Centrales/uso terapéutico , Espasticidad Muscular/tratamiento farmacológico , Adolescente , Baclofeno/administración & dosificación , Niño , Preescolar , Estudios de Seguimiento , Humanos , Inyecciones Espinales , Monitoreo Intraoperatorio , Relajantes Musculares Centrales/administración & dosificaciónRESUMEN
OBJECT: Because appropriate patient selection is essential for achieving successful outcomes after epilepsy surgery, the need for more robust methods of predicting postoperative seizure control has been created. Standard multivariate techniques have been only 75 to 80% accurate in this regard. Recent use of artificial intelligence techniques, including neural networks, for analyzing multivariate clinical data has been successful in predicting medical outcome. METHODS: The authors applied neural network techniques to 80 consecutive patients undergoing epilepsy surgery in whom data on demographic, seizure, operative, and clinical variables to predict postoperative seizures were collected. Neural networks could be used to predict postoperative seizures in up to 98% of cases. Student's t-tests or chi-square analysis performed on individual variables revealed that only the preoperative medication index was significantly different (p = 0.02) between the two outcome groups. Six different combinations of input variables were used to train the networks. Neural network accuracies differed in their ability to predict seizures: using all data (96%); all data minus electroencephalography concordance and operative side (93%); all data except intra- or postoperative variables such as tissue pathological category (98%); all data excluding pathological category, intelligence quotient (IQ) data, and Wada results (84%); only demographics and tissue pathological category (65%); and only IQ data (63%). CONCLUSIONS: Analysis of the results reveals that several networks that are trained with the usual accepted variables characterizing the typical evaluation of epilepsy patients can predict postoperative seizures with greater than 95% accuracy.
Asunto(s)
Anticonvulsivantes/uso terapéutico , Epilepsia/tratamiento farmacológico , Epilepsia/cirugía , Redes Neurales de la Computación , Cuidados Preoperatorios , Electroencefalografía , Epilepsia/diagnóstico , Epilepsia/patología , Epilepsia/psicología , Predicción , Humanos , Pruebas Neuropsicológicas , Complicaciones Posoperatorias , Convulsiones/etiología , Resultado del TratamientoRESUMEN
Positron emission tomography (PET) with rubidium-82 (82Rb) and fluorine-18-fluorodeoxyglucose (18F-FDG) was used to diagnose active tumor recurrence and to differentiate this from radiation injury after interstitial irradiation of malignant gliomas. Patients were studied when they presented with radiological or clinical deterioration after an initial period of posttreatment stabilization. Forty studies were performed in 34 patients. The 82Rb was used as a blood-brain barrier tracer to localize the lesion. Uptake of 18F-FDG by the lesion was then compared to uptake by adjacent brain in order to make a diagnosis of active tumor recurrence (higher or equal lesion uptake) or no active tumor (lower uptake). Radiation injury was diagnosed by the exclusion of active tumor. A retrospective clinical diagnosis was established in 38 cases by following the patients' progress for 8 to 142 weeks after the PET study. In two cases, no follow-up diagnosis could be determined. The PET results agreed with the follow-up diagnosis in 15 of 17 cases of active tumor and 17 of 21 cases of radiation injury. Histological examination of surgically resected tissue obtained after the PET study was performed in 18 patients (nine with tumor regrowth and nine with radiation injury). This showed apparently viable tumor as well as necrosis in all cases, regardless of eventual clinical outcome. Some cells from the irradiated volume may appear morphologically intact, but have little or no metabolic or clinical activity. The functional nature of the PET-FDG technique allows diagnosis of tumor activity, which cannot be demonstrated by anatomic imaging studies or by histological examination. The addition of a blood-brain barrier tracer to the 18F-FDG study aids in differentiating normal brain uptake from tumor activity and improves the accuracy of the technique.
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Astrocitoma/radioterapia , Braquiterapia , Neoplasias Encefálicas/radioterapia , Glioblastoma/radioterapia , Glioma/radioterapia , Tomografía Computarizada de Emisión , Astrocitoma/diagnóstico por imagen , Astrocitoma/metabolismo , Neoplasias Encefálicas/diagnóstico por imagen , Neoplasias Encefálicas/metabolismo , Desoxiglucosa/análogos & derivados , Desoxiglucosa/metabolismo , Diagnóstico Diferencial , Radioisótopos de Flúor , Fluorodesoxiglucosa F18 , Glioblastoma/diagnóstico por imagen , Glioblastoma/metabolismo , Glioma/diagnóstico por imagen , Glioma/metabolismo , Humanos , Recurrencia Local de Neoplasia/diagnóstico , Traumatismos por Radiación/diagnóstico , Radioisótopos de RubidioRESUMEN
We reviewed the seizure frequencies of 38 patients with medically refractory epilepsy 6 weeks before (baseline) and 3 weeks after withdrawing their antiepileptic drugs (AEDs) for video-EEG monitoring. Seizure frequency during the first 3 weeks after restarting AEDs was diminished compared with baseline (P < 0.05). We found no correlation between seizure frequency and patient age, specific antiepileptic drugs, number of seizures during the video-EEG monitoring, number of days without AEDs, or partial vs total withdrawal of AEDs. Medication tachyphylaxis, functional tolerance, or long term post-ictal depression of the seizure threshold is hypothesized. The diminished seizure frequency after reinstitution of medications in four of five patients who did not have seizures during their hospitalization suggests that drug tachyphylaxis is a relevant mechanism.
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Anticonvulsivantes/efectos adversos , Electroencefalografía/efectos de los fármacos , Epilepsias Parciales/tratamiento farmacológico , Síndrome de Abstinencia a Sustancias/fisiopatología , Adolescente , Adulto , Anticonvulsivantes/administración & dosificación , Esquema de Medicación , Quimioterapia Combinada , Tolerancia a Medicamentos , Epilepsias Parciales/fisiopatología , Potenciales Evocados/efectos de los fármacos , Potenciales Evocados/fisiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Monitoreo Fisiológico , Procesamiento de Señales Asistido por Computador , Síndrome de Abstinencia a Sustancias/tratamiento farmacológico , Grabación en VideoRESUMEN
Our experience with a very low end interactive image-directed (IIDS) neurosurgical system is presented. The system was developed by the author and consists of a personal desktop computer and a magnetic field digitizer. This low cost solution was pursued as an alternative to available commercial devices which were expensive and not readily modifiable for novel ideas and new applications targeted for Epilepsy surgery. The rationale and description of the system was presented last year at Medicine Meets Virtual Reality III. Included in that detailed report were the fundamental mathematics forming the basics of transformation between the surgical and the digital data spaces. Since then the system has been used in an additional 20 cases now totaling 40 in all. Its advantages and short comings will be described. The theoretical advantages of magnetic field technology over other localization methods is reviewed. Also, our experience with alternative low cost off-the-shelf interfacing devices and other related modifications are described. We have accumulated clinical data to suggest that craniotomy sizes have been reduced, electrode placement has been improved, and that interactive image-directed techniques offer advantages over other common intra-operative localization modalities such as ultrasound. Our conclusion is that interactive image-directed techniques improve neurosurgery and that inexpensive enabling technology is already available providing the technological substrate for low cost devices using virtual reality notions for surgery and medicine. This particular technology offers advantages to traditional surgical techniques demonstrating the attractiveness of rudimentary virtual reality medical applications.
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Simulación por Computador , Diagnóstico por Imagen/instrumentación , Epilepsia/cirugía , Procesamiento de Imagen Asistido por Computador/instrumentación , Neurocirugia/instrumentación , Técnicas Estereotáxicas/instrumentación , Mapeo Encefálico/instrumentación , Neoplasias Encefálicas/cirugía , Periféricos de Computador , Craneotomía/instrumentación , Humanos , MicrocomputadoresRESUMEN
Right hemisphere functions were examined during cortical stimulation in six patients undergoing epilepsy surgery. Two patients showed mild hemispatial neglect and constructional disability with stimulation of several sites in the inferior parietal lobule and posterior temporal lobe. Two other patients showed no disruption of visuospatial functions with stimulation of similar parietal or posterior temporal sites. Nonverbal acoustic perception of environmental sounds was not disrupted with stimulation of right superior temporal cortex in one patient, and musical abilities were not affected by right superior temporal stimulation in another patient. Nondominant hemisphere functions may be more widely distributed and less localized than linguistic functions in the dominant hemisphere.
RESUMEN
Few studies have examined the clinical features of neocortical temporal lobe epilepsy (NTLE) in carefully selected patients. We reviewed records from 21 patients with NTLE, defined by intracranial electroencephalogram (EEG), who have been seizure free for 1 year or more following temporal lobectomy. The mean age of onset at the time of first seizure was 14 years (range, 1-41 years). Febrile seizures were reported in only 2 patients (9.5%). In contrast to prior mesial temporal lobe epilepsy (MTLE) studies, seizure-free intervals between the initial cerebral insult or first seizure and habitual seizures were uncommon. Possible or known risk factors for epilepsy were reported in 13 of 21 patients (62%). Fifteen (71%) patients reported auras, with experiential phenomena being the most common type. Magnetic resonance imaging was normal or nonspecific in 15 patients, revealed mild hippocampal atrophy in 2, tumors in 2, and heterotopic gray matter and hippocampal atrophy in 1, and cortical dysgenesis in 1. Neuropsychological testing showed deficits consistent with the seizure focus in 13 patients (62%), and Wada test showed ipsilateral memory deficits in 10 (48%). The most common behavioral manifestation was a motionless stare at ictal onset (48%). In contrast to prior studies of MTLE, only 1 NTLE patient had frequent independent, contralateral temporal lobe epileptiform spikes on scalp EEG.
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Encéfalo/patología , Electroencefalografía , Epilepsia del Lóbulo Temporal/fisiopatología , Epilepsia del Lóbulo Temporal/cirugía , Adolescente , Adulto , Edad de Inicio , Supervivencia sin Enfermedad , Epilepsia del Lóbulo Temporal/patología , Femenino , Fiebre , Humanos , Imagen por Resonancia Magnética , Masculino , Pruebas Neuropsicológicas , Estudios Retrospectivos , Factores de Riesgo , ConvulsionesRESUMEN
The phosphorylation state of the proteins, regulated by phosphatases and kinases, plays an important role in signal transduction and long-term changes in neuronal excitability. In neurons, cAMP-dependent protein kinase (PKA), protein kinase C (PKC) and calcineurin (CN) are attached to a scaffold protein, A kinase anchoring protein (AKAP), thought to anchor these three enzymes to specific sites of action. However, the localization of AKAP, and the predicted sites of linked phosphatase and kinase activities, are still unknown at the fine structural level. In the present study, we investigated the distribution of AKAP79 in the hippocampus from postmortem human brains and lobectomy samples from patients with intractable epilepsy, using preembedding immunoperoxidase and immunogold histochemical methods. AKAP79 was found in the CA1, presubicular and subicular regions, mostly in pyramidal cell dendrites, whereas pyramidal cells in the CA3, CA2 regions and dentate granule cells were negative both in postmortem and in surgical samples. In some epileptic cases, the dentate molecular layer and hilar interneurons also became immunoreactive. At the subcellular level, AKAP79 immunoreactivity was present in postsynaptic profiles near, but not attached to, the postsynaptic density of asymmetrical (presumed excitatory) synapses. We conclude that the spatial selectivity for the action of certain kinases and phosphatases regulating various ligand- and voltage-gated channels may be ensured by the selective presence of their anchoring protein, AKAP79, at the majority of glutamatergic synapses in the CA1, but not in the CA2/CA3 regions, suggesting profound differences in signal transduction and long-term synaptic plasticity between these regions of the human hippocampus.
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Proteínas Adaptadoras Transductoras de Señales , Proteínas Portadoras/análisis , Hipocampo/química , Proteínas de Anclaje a la Quinasa A , Animales , Anticuerpos , Calcineurina/análisis , Proteínas Portadoras/inmunología , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Dendritas/química , Dendritas/enzimología , Dendritas/ultraestructura , Epilepsia/metabolismo , Femenino , Ácido Glutámico/fisiología , Hipocampo/citología , Humanos , Técnicas para Inmunoenzimas , Inmunohistoquímica , Interneuronas/química , Interneuronas/enzimología , Interneuronas/ultraestructura , Masculino , Microscopía Electrónica , Células Piramidales/química , Células Piramidales/enzimología , Células Piramidales/ultraestructura , Conejos , Sinapsis/química , Sinapsis/enzimología , Sinapsis/ultraestructura , Adhesión del Tejido , Ácido gamma-Aminobutírico/fisiologíaRESUMEN
Nine radiation-treated brain tumor patients were studied by positron emission tomography (PET) in an attempt to differentiate tumor recurrence from radiation necrosis. Rubidium-82 was used to define the region of absent or disturbed blood-brain barrier and [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) was used to evaluate the metabolic state of the brain. By comparing glucose utilization in the pathologic region with utilization in the adjacent tissue, a diagnosis of recurrent tumor (increased [18F]FDG accumulation) or necrosis (decreased FDG accumulation) was made. In the seven patients who underwent surgery the PET diagnosis was confirmed by histologic examination of resected tissue. The two patients who did not undergo surgery have had a clinical course consistent with the PET diagnosis of necrosis. Dynamic 82Rb imaging showed that the rate of 82Rb accumulation was greater in tumor than in normal brain. However, this finding alone did not differentiate tumor from necrosis, as some necrotic tissue also showed high rates of 82Rb accumulation, and washout kinetics were similarly nonspecific. The differentiation of radiation necrosis from tumor recurrence is reliably achieved by [18F]FDG PET examination and is aided by information obtained from a 82Rb PET study done immediately prior to the [18F]FDG injection.