RESUMEN
Brassinosteroids are a new group of phytohormones that are widely distributed in plants and play an important role in the processes of plant growth and development. Physiological concentrations of brassinosteroids in plants are extremely low, and their analysis in organs and tissues is very difficult. This study is devoted to the chemical aspects of elaboration and to bioanalytical parameters of an immunoenzymatic system for quantitative determination of the phytohormones 24-epicastasterone and 24-epibrassinolide.
Asunto(s)
Colestanoles/química , Reguladores del Crecimiento de las Plantas/química , Esteroides Heterocíclicos/química , Animales , Antígenos de Plantas/análisis , Antígenos de Plantas/inmunología , Brasinoesteroides , Colestanoles/análisis , Colestanoles/inmunología , Reacciones Cruzadas , Sueros Inmunes/aislamiento & purificación , Técnicas para Inmunoenzimas , Reguladores del Crecimiento de las Plantas/inmunología , Conejos , Esteroides Heterocíclicos/análisis , Esteroides Heterocíclicos/inmunologíaRESUMEN
A number of new steroidal 17-spirofuran derivatives of the 19-nor series containing Me, Et or (i)Pr-substituents in the heterocyclic moiety has been prepared, which are expected to have a strong progestagenic activity. The proposed approach made use of the 1-3-dipolar cycloaddition of low-molecular nitrile oxides with steroidal acetylenic alcohols followed by transformation of the isoxazole side chain.