RESUMEN
OBJECTIVES: To investigate the value of 2-dimensional (2D) speckle-tracking echocardiography for assessing right ventricular (RV) systolic function in patients with chronic pulmonary heart disease (CPHD) and the correlation of its parameters with the right ventricular ejection fraction (RVEF) on cardiac magnetic resonance imaging (MRI). METHODS: According to pulmonary arterial systolic pressure, 80 patients with CPHD and tricuspid regurgitation were divided into 2 groups: 42 with mild pulmonary hypertension (PH; 30-50 mm Hg) and 38 with moderate or severe PH (≥50 mm Hg); 41 control participants were recruited. All participants underwent 2D speckle-tracking echocardiography and cardiac MRI. The longitudinal peak systolic strain and longitudinal peak systolic strain rate were measured by echocardiography in each segment of the RV free wall and interventricular septum and compared with the RVEF on cardiac MRI. RESULTS: Strain values in all segments of the RV free wall and interventricular septum were lower in the mild PH group than the control group (P < .05). Strain rate values in the apical segment of the RV free wall and basal segment of the interventricular septum were lower in the mild PH group than the control group (P< .05). Strain and strain rate values in all segments of the RV free wall and interventricular septum were lower in the moderate or severe PH group than the control group (P < .05). Strain and strain rate values in all segments of the RV free wall and interventricular septum were lower in the moderate or severe PH group than the mild PH group (P< .05). Strain and strain rate values in all segments of the RV free wall and the interventricular septum correlated with the RVEF (P < .001). CONCLUSIONS: The ability of speckle-tracking echocardiography to directly monitor RV myocardial function may allow early sensitive detection of subclinical myocardial dysfunction in patients with CPHD, with better risk stratification and timely institution of therapy.
Asunto(s)
Ecocardiografía , Enfermedad Cardiopulmonar/complicaciones , Disfunción Ventricular Derecha/complicaciones , Disfunción Ventricular Derecha/diagnóstico por imagen , Adulto , Anciano , Enfermedad Crónica , Femenino , Ventrículos Cardíacos/diagnóstico por imagen , Ventrículos Cardíacos/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Enfermedad Cardiopulmonar/fisiopatología , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Disfunción Ventricular Derecha/fisiopatologíaRESUMEN
OBJECTIVES: To investigate the effect of acupuncture at PC6 on cardiac hypertrophy in isoproterenol (ISO)-treated mice. METHODS: 48 male C57BL/6 mice underwent subcutaneous injection of ISO for 14 days and were randomly divided into four groups (n=12 each) that remained untreated (ISO group), received verum manual acupuncture (MA) treatment at PC6 (ISO+MA(PC6) group), sham MA at location on the tail not corresponding to any traditional acupuncture point (ISO+MA(tail) group), or propranolol (ISO+PR group). An additional 12 mice were given an injection of phosphate-buffered saline (PBS) and formed a healthy control (Normal) group. After performing echocardiography and measuring the ratio of heart weight (HW)/tibia length (TL) at 14 days, all mice were euthanased. Morphological examination was performed following haematoxylin and eosin and Masson's staining of heart tissues. Ultrastructural changes were observed by electron microscopy. Cardiac protein expression of atrial natriuretic peptide (ANP) and tumour necrosis factor α (TNFα) were measured by immunohistochemical (IHC) staining and Western blotting. RESULTS: Compared with the untreated model group, acupuncture at PC6 lowered the heart rate, reduced the ratio of HW/TL, improved the left ventricular (LV) anterior wall thickness (LVAWd), LV end-diastolic anterior wall thickness (LVAWs), LV end-systolic posterior wall thickness (LVPWd), LV end-diastolic posterior wall thickness (LVPWs), and fractional shortening (FS) as observed by echocardiography (ISO+MA(PC6) vs. ISO groups: P<0.05). Moreover, evidence from morphological studies demonstrated that acupuncture at PC6 inhibited myocardial hypertrophy and collagen deposition, and normalised the ultrastructural changes. In addition, ANP and TNFα expression were attenuated in the verum acupuncture group compared with the untreated model group (ISO+MA(PC6) vs. ISO groups: P<0.05). CONCLUSIONS: The results demonstrated that acupuncture at PC6 attenuates sympathetic overactivity. Additionally, it may improve cardiac performance by reversing adverse cardiac remodelling. Acupuncture has potential as a treatment for sympathetic hypertension.
Asunto(s)
Terapia por Acupuntura , Cardiomegalia/terapia , Isoproterenol/efectos adversos , Puntos de Acupuntura , Animales , Factor Natriurético Atrial/genética , Factor Natriurético Atrial/metabolismo , Cardiomegalia/inducido químicamente , Cardiomegalia/tratamiento farmacológico , Cardiomegalia/metabolismo , Modelos Animales de Enfermedad , Humanos , Inyecciones Subcutáneas , Isoproterenol/administración & dosificación , Masculino , Ratones , Ratones Endogámicos C57BL , Propranolol/administración & dosificación , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
The 4[(Tetrahydro2Hpyran2yl) oxy] phenol (XGd) hydroquinone analog, is found in Vaccinium vitisidaea L. Although it is known for its antioxidant properties and high level of safety, its antitumor activity remains to be elucidated. In the present study, the anticancer effect of XGd was determined in vitro and in vivo. The cytotoxicity of XGd against C26 murine colon carcinoma cells was found to occur in a time and concentrationdependent manner, whereas little effect was observed in the two normal cell lines (HK2 and L02) investigated. Oral administration of XGd (100 mg/kg) had effects on the tumor growth of tumorbearing mice. Furthermore, marked apoptosis was observed using Hoechst 33258 staining and flow cytometric analysis with annexin V/propidium iodide double staining. XGd also downregulated the expression of Bcell lymphoma 2 (Bcl2), increased the expression levels of Bcl2associated X protein and activated caspase9, caspase3 and poly(adenosine diphosphateribose) polymerase. The present study demonstrated for the first time, to the best of our knowledge, that XGd inhibited cancer cell growth via the induction of apoptosis and was also able to inhibit tumor growth in vivo. These results demonstrated that XGd may be used as a potential natural agent for cancer therapy with low toxicity.
Asunto(s)
Antineoplásicos Fitogénicos/toxicidad , Proliferación Celular/efectos de los fármacos , Hidroquinonas/química , Fenoles/toxicidad , Piranos/toxicidad , Administración Oral , Animales , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/uso terapéutico , Apoptosis/efectos de los fármacos , Caspasa 3/metabolismo , Caspasa 9/metabolismo , Línea Celular Tumoral , Neoplasias del Colon/tratamiento farmacológico , Neoplasias del Colon/metabolismo , Neoplasias del Colon/patología , Femenino , Puntos de Control de la Fase G1 del Ciclo Celular/efectos de los fármacos , Células HT29 , Humanos , Hidroquinonas/uso terapéutico , Hidroquinonas/toxicidad , Ratones , Ratones Endogámicos BALB C , Microscopía Electrónica de Transmisión , Fenoles/química , Fenoles/uso terapéutico , Poli(ADP-Ribosa) Polimerasas/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Piranos/química , Piranos/uso terapéutico , Vaccinium/química , Vaccinium/metabolismo , Ensayos Antitumor por Modelo de Xenoinjerto , Proteína X Asociada a bcl-2/metabolismoRESUMEN
Drug-resistant bacterial infections and lack of available antibacterial agents in clinical practice are becoming serious risks to public health. We synthesized a new class of haloemodins by modifying a traditional Chinese medicine component, emodin. The novel haloemodin exerts strong inhibitory activity on bacterial topoisomerase I and DNA gyrase, and not on the topoisomerases of human origin. In principle, it shows remarkable antibacterial activities against laboratory and clinically isolated Gram-positive bacteria, including vancomycin-resistant Enterococcus faecium and methicillin-resistant Staphylococcus aureus. We further expanded its antibacterial spectrum into against Gram-negative bacteria with the assistance of polymyxin B nonapeptide, which helps haloemodin to penetrate through the bacterial outer membrane. Finally, the therapeutic effect of haloemodin in vivo was confirmed in curing S. aureus-induced keratitis on rabbit model. With distinctive structural difference from the antibiotics we used, the haloemodins are of value as promising antibacterial pharmacophore, especially for combat the infections caused by drug-resistant pathogens.