Natural sanitizer potential of Cuminum cyminum and applicable approach for calculation of Kováts retention index of its compounds.

The demand for natural agents instead of chemicals in terms of food and health safety is increasing day by day. This study aimed to investigate the potential of the methanolic extract of Cuminum cyminum (C. cyminum) in the fight against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus)and Candida (C. albicans). The chemical composition of the methanolic extract of C. cyminum was analyzed using GS-MS. Also, Kováts retention indices were calculated for the detected compounds using an applicable formula. The most basic substance was cuminic aldehyde (27.86%) and p-(Dimethoxymethyl)-isopropylbenze (18.32%). The Minimum Inhibitory Concentration (MIC) of the extract was 0.1 g/mL for S. aureus and C. albicans while it was > 0.1 for E. coli. Although the methanol extract of C. cyminum acts against all three microorganisms, the most lasting effect was on S. aureus, indicating that it can be recommended as a strong antibacterial disinfectant for S. aureus.

Antimicrobial activity of Stachys rupestris Montbret et Aucher ex Benth. and inactivation of the pathogens inoculated on lab-made skin by the essential oil.

The aim of this study was to investigate the antimicrobial activity of Stachys rupestris essential oil and inactivation of the pathogens on lab-made skin the oil in the fight against Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, Candida albicans and Candida tropicalis. S. rupestris EO was extracted by hydrodistillation, and its contents were analyzed by GCMS. Logarithmic reduction of the pathogens inoculated on the artificial skin surface by S. rupestris EO was studied for the first time. The highest inhibition zone was 22.1 mm on C. tropicalis, while the lowest IZ was 0.1 mm on E. coli. The other zones were 20.01 mm for Acinetobacter baumannii, 20.02 mm for Enterococcus faecalis, 20.01 mm for Staphylococcus aureus, 22.03 for Candida albicans (p < 0.05). As a result, S. rupestris essential oil was effective on most of the microorganisms and might be increased to use in the treatment of skin infections in the future.

Antibacterial activity and antibacterial mechanism of ethanol extracts of Lentinula edodes (Shiitake) and Agaricus bisporus (button mushroom).

The aim of the present work was the comparison of antimicrobial activity, mechanism and components of the ethanol extract (EE) from Lentinula edodes Berk (Pegler) and Agaricus bisporus Sing (Lange). The main component of EE of A. bisporus was dianhydromannitol (20.1%), while isosorbide/dianhydromannitol (21.8%) was detected at a high rate in L. edodes ethanol extract by GC-MS . The common phenolic acids were determined as chlorogenic acid, syringic acid, rutin, p-coumaric acid, ferulic acid, 2-hydroxy cinnamic acid, protocatechuic acid, abscisic acid, and trans-cinnamic acid in both mushroom extract by HPLC-MWD . The MICs (minimum inhibitory concentration) of L. edodes EE on Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Acinetobacter baumannii were between 5.1 mg ml-1 and 6.01 mg ml-1, while MICs of A. bisporus EE on the pathogens were between 5.8 mg ml-1 and 9.54 mg ml-1. The highest DRA decrease was in E. faecalis (69.1%) for L. edodes and S. aureus (71.0%) for A. bisporus in the 20th minute. As a result, L. edodes and A. bisporus have a similar antibacterial effect on the pathogens, and this inhibition effect caused DNA, protein leakage and destruction of permeability of bacterial cell membrane by bioactive molecules in mushroom extract.

Evaluation of Citrus reticulata essential oil: Chemical composition and antibacterial effectiveness incorporated gelatin on E. coli and S. aureus.

In the present study, we evaluated the antibacterial activity of Citrus reticulata (C. reticulata) essential oil-incorporated gelatin film solution against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The minimum inhibitory concentration (MIC) of C. reticulata essential oil (CrEO) on E. coli and S. aureus were found to be 10.1 and 9.1 mg mL-1, respectively, using spectrophotometric microdilution technique. The antimicrobial effect of CrEO alone and incorporated gelatin-based film solutions (CrEO-F) against these pathogens were determined by the disc diffusion method. While the inhibition zones of CrEO were 2.4 mm and 10.05 mm, CrEO-F was reported as 1.5 and 7.8 mm against E. coli and S. aureus, respectively. These findings demonstrated that the CrEO can compete with coating agents with antimicrobial additives and it can find a place in the application areas. Besides, the chemical composition of the CrEO was determined by GC-MS.

Chemical composition, antifungal activity, antifungal mechanism and interaction manner of the fatty acid of Prunus mahaleb L. with fluconazole.

The interaction manner of Prunus mahaleb L. (P. mahaleb) seed oil (MSO) and fluconazole (FLC: antimycotic) combinations (MSO*FLC) against C. albicans and C. parapsilosis were evaluated using the microdilution technique. The most representative compounds of mahaleb oil were found to be conjugated linolenic acid (34.39%), oleic acid (31.76%), and linoleic acid (25.54%) by GC-MS. In antimicrobial activity study, P. mahaleb had an inhibition zone (IZ) of C. albicans and C. parapsilosis with 6.89 mm and 11.39 mm and a minimum inhibitory concentration (MIC) with 35.3 µgmL-1 and 23.9 µgmL-1, respectively. The strongest indifferent effect was observed as 57.14% for C. albicans and 100% for C. parapsilosis in fluconazole-mahaleb oil combinations. An increase in DNA and protein leakage was observed when yeast was exposed to the FA. The destruction on the cell surface was visualized using Scanning Electron Microscopy (SEM) analysis.

Antimicrobial Activity and Mechanism of Essential Oil of Endemic Salvia hypargeia Finc. & Mey. in Turkey.

In this study, the aerial parts of Salvia hypargeia were subjected to hydrodistillation and the resulting compounds were analyzed in GC-MS. Antimicrobial activity of S. hypargeia essential oil (EO) against A. baumannii , S. aureus, and C. tropicalis were determined by agar well diffusion assay and microdilution method. Antimicrobial mechanism of the EO were investigated based on change of TTC-dehydrogenase relative, leakages of intracellular protein, DNA and potassium ion (K +). The main components of the EO were ß-pinene, 1,8-cineole, camphor, α-pinene, 4-terpineol, and 4-thujanol. The MICs of the EO against the microorganisms were 15.2 mg/mL for S. aureus, 17.5 mg/mL for C. tropicalis and 28.8 mg/mL for A. baumannii. The inhibition zones were 18.16 mm, 25.01 mm, and 27.01 mm for A. baumannii, S. aureus, and C. tropicalis, respectively (p < 0.05). An increase in DNA, protein and K+ leakage was observed when microorganisms were exposed to the EO. The TTC-DRA of the treated microorganism cells was also significantly decreased because of slowing the respiration. The present study provided an experimental basis of practical application of S. hypargeia EO as a natural agent. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-021-00939-1.

Green synthesis of walnut shell hydrochar, its antimicrobial activity and mechanism on some pathogens as a natural sanitizer.

Hydrochar of waste walnut shells (WSH) was synthesized in the eco-friendly subcritical water medium (SWM) and its potential to fight against Klebsiella pneumoniae (K. pneumoniae), Staphylococcus aureus (S. aureus), Candida albicans (C. albicans) and Candida parapsilosis (C. parapsilosis) was investigated. Minimum Inhibitory Concentration (MIC) values of the WSH were 3.01 g/mL, 2.06 g/mL, 1.95 g/mL, and 3.12 g/mL for K. pneumoniae, S. aureus, C. albicans and C. parapsilosis, respectively. Survival of the pathogens was investigated by 3 min surface disinfection test exposure to WSH. While the highest inhibition was seen for C. parapsilosis (96.67%) on paper surface with 0.3 g/mL of bovine serum albumin (BSA), the lowest inhibition was determined for C. albicans (6.44%) on the plastic glass surface with 3 g/mL of BSA. An increase in protein, DNA, and potassium ion (K+) leakage was observed after microorganisms were incubated with WSH. This study provided an experimental basis for the practical application of WSH as a natural sanitizer agent.