Fabrication and characterization of gold nano-wires templated on virus-like arrays of tobacco mosaic virus coat proteins.

The rod-shaped plant virus tobacco mosaic virus (TMV) is widely used as a nano-fabrication template, and chimeric peptide expression on its major coat protein has extended its potential applications. Here we describe a simple bacterial expression system for production and rapid purification of recombinant chimeric TMV coat protein carrying C-terminal peptide tags. These proteins do not bind TMV RNA or form disks at pH 7. However, they retain the ability to self-assemble into virus-like arrays at acidic pH. C-terminal peptide tags in such arrays are exposed on the protein surface, allowing interaction with target species. We have utilized a C-terminal His-tag to create virus coat protein-templated nano-rods able to bind gold nanoparticles uniformly. These can be transformed into gold nano-wires by deposition of additional gold atoms from solution, followed by thermal annealing. The resistivity of a typical annealed wire created by this approach is significantly less than values reported for other nano-wires made using different bio-templates. This expression construct is therefore a useful additional tool for the creation of chimeric TMV-like nano-rods for bio-templating.

A stokes polarimetric light microscopy view of liquid crystal droplets.

The optical characteristics of materials, such as their magnetooptical effects, birefringence, optical activities, linear and circular dichroism, are probed via the polarisation states of light transmitted through or reflected from the specimens. As such, the measurements of the polarisation states play an important role in many research disciplines. Experimentally, Stokes parameters provide a full description of the polarisation states of light. We report the implementation of a dual- photoelastic modulator based polarimeter in a light microscope, enabling the determination of Stokes parameters at each pixel. As a case study, polarimetric images of liquid crystal droplets of different internal structures are obtained, showing their distinct polarisation characteristics. We demonstrate that the prototype Stokes polarimetric microscope allows the quantitative determination of the polarisation characteristics of light at the object plane and enables the access of the information of full polarisation states as compared to a conventional cross polariser microscope. This work shows that Stokes polarimetric microscopy may find potential applications in a wide range of research fields.

Rational screening of biomineralisation peptides for colour-selected one-pot gold nanoparticle syntheses.

Biomineralisation peptides that facilitate the one-pot synthesis of gold nanoparticles (AuNPs) with selected optical properties, were screened using a coherent peptide-spotted array consisting of a AuNP binding peptide library. As the biomineralised AuNPs were captured on each peptide spot, analysis of the images provided information on their collective optical properties.

Controlling transmembrane protein concentration and orientation in supported lipid bilayers.

The trans-membrane protein - proteorhodopsin (pR) has been incorporated into supported lipid bilayers (SLB). In-plane electric fields have been used to manipulate the orientation and concentration of these proteins, within the SLB, through electrophoresis leading to a 25-fold increase concentration of pR.

The physical demands of Super League rugby: Experiences of a newly promoted franchise.

The physical match demands for a newly promoted European Super League (ESL) squad were analysed over a full season using global positioning systems. Players were classified into four positional groups: outside backs (OB), pivots (PIV), middle unit forwards (MUF) and wide running forwards (WRF). MUF covered less total distance (4318 ± 570 m) than WRF (6408 ± 629 m), PIV (6549 ± 853) and OB (7246 ± 333 m) (P < 0.05) and less sprint distance (185 ± 58 m) than WRF (296 ± 82 m), PIV (306 ± 108) and OB (421 ± 89 m; P < 0.05), likely attributable to less playing time by MUF (47.8 ± 6.6 min) compared with WRF (77.0 ± 9.0 min), PIV (72.8 ± 10.6 min) and OB (86.7 ± 3.4 min; P < 0.05). Metres per minute were greater for MUF (90.8 ± 2.2 m.min(-1)) compared with OB (83.6 ± 2.8 m.min(-1)) and WRF (83.4 ± 2.4 m.min(-1); P = 0.001) although not different from PIV (90.2 ± 3.3 m.min(-1); P > 0.05). WRF (36 ± 5) and MUF (35 ± 6) were involved in more collisions than OB (20 ± 3) and PIV (23 ± 3; P < 0.05). The high-speed running and collision demands observed here were greater than that previously reported in the ESL, which may reflect increased demands placed on the lower ranked teams. The present data may be used to inform coaches if training provides the physical stimulus to adequately prepare their players for competition which may be especially pertinent for newly promoted franchises.

Discrete membrane arrays.

This review describes various methods for the attachment of phospholipid bilayers to solid supports. The simplest approach involves vesicle unrolling onto a surface that has been previously modified with a continuous self-assembled monolayer (SAM). The choice of a suitable SAM can lead to the formation of attached bilayers that have the desired biomimetic properties and are suitable for studying transmembrane proteins. However, there are intrinsic problems associated with this approach if one is interested in studying ion transport phenomena. In particular, the relatively low resistance values found for such bilayers do not permit studies of single ion channels. For such studies to be carried out the background leakage through the lipid film must be greatly reduced. In an attempt to reduce the problems of leakage we have formed patterned SAMs in which a blocking, hydrophobic, layer covers 90% of the electrode surface. The remaining portion of the surface, which is hydrophilic, supports the formation of a bilayer. This approach has led to an improvement in the quality of the bilayers formed but has still not provided bilayers with sufficiently high specific resistances to study single ion channels. Finally, we describe new approaches based on the formation of bilayers suspended over small apertures. These 'suspended' bilayers are similar in structure to those used in black lipid membrane experiments and give rise to highly blocking bilayer membranes. Unfortunately, this approach requires the use of solvents to create the suspended bilayer and they are relatively fragile.

Measurement of textural changes of food by MRI relaxometry.

This article summarises the current status of, and future prospects for, the use of magnetic resonance imaging (MRI) to evaluate the texture of a range of foods, and changes therein which accompany pathogen infection, natural ageing, damage, ripening and processing. The basic concept is that the magnetic resonance parameters of water are sufficiently sensitive to the texture of the food matrix, that magnetic resonance images of the spatial distribution of those parameters are effectively maps of the structural status of the foodstuff. This is illustrated in the context of the effects of pathogen infection in cucumbers, internal necrosis of melons, bruising in peaches, ripening of pineapples, and the effect of freeze-thawing on meat and fish.

Quantitative magnetic resonance imaging of fresh and frozen-thawed trout.

Magnetic resonance imaging (MRI) has been used to visualise the major organs and muscular-skeletal frame-work of fresh rainbow trout (Salmo gairdneri) in two dimensions, and to identify the spatial distribution of lipid- and collagen-rich tissues. Quantitative MRI provides the MR parameters (T1, T2, M0, T1sat, Msat/M0, and the Magnetisation Transfer (MT) rate) for the tissue water; variations in those parameters enable distinction to be made between a freshly killed trout and one which has been frozen-thawed. The effects of freezing method, repeat freeze-thawing, and storage time on the MR parameters are discussed.

Drug-induced hyperthermia.

Drug-related causes of hyperthermia can often be overlooked in the setting of elevated body temperature. This article reviews the pathophysiology, presentation, and treatment of several drug-induced hyperthermia syndromes: malignant hyperthermia, neuroleptic malignant syndrome, sympathomimetic poisoning, and anticholinergic toxicity. Although the general approach is similar, specific management strategies may be required for each syndrome.

Surface energy of ethylene-co-1-butene copolymers determined by contact angle methods.

Wilhelmy plate measurements of contact angles with a series of test liquids are used to calculate the surface energies of two poly(ethylene-co-1-butene) random copolymers. Results from five methods of calculation are reported: one-liquid (Good-Girifalco and Neumann), two-liquid (harmonic mean and geometric mean), and three-liquid (Lifshitz-van der Waals acid-base) methods. We find that all five methods are sensitive to the choice of test liquids used for contact angle measurements, as previously reported, but consistent results are obtained if recommended combinations of liquids are used. The mean results of the three-liquid acid-base method are judged to be the most reliable and informative, leading to surface energies of 30.8 mJ/m2 for poly(ethylene-co-1-butene) copolymer composed of 92 mol% ethylene and 30.2 mJ/m2 for copolymer composed of 88 mol% ethylene.

Chemical manipulation by X-rays of functionalized thiolate self-assembled monolayers on Au.

The chemical modification caused by prolonged exposure to X-rays on a series of para-substituted phenyl moieties (-NO2, -CN, -CHO, -COOH, -CO2Me, and -CO2(1)Bu) at the surface of thiolate-Au self-assembled monolayers (SAMs) has been investigated by X-ray photoelectron spectroscopy (XPS). Furthermore, the influence that the phenyl group has on the chemical modification induced by the X-ray irradiation on the SAMs was investigated by comparing the XPS results obtained from irradiation on a NO2-aromatic-terminated SAM (6-(4-nitro-phenoxy)-hexane-1-thiolate (NPHT)) and NO2-aliphatic-terminated SAM (thioacetic acid S-(12-nitrododecyl) ester (TNDDE)). The NPHT and TNDDE SAMs have been shown to behave differently to X-ray exposure. The irradiation of the NPHT SAM led to the reduction of the nitro (-NO2) moiety to the amine (-NH2) moiety, as shown by the decrease in the intensity of the N 1s photoelectron peak for -NO2 (406 eV) in the XPS spectra with the concomitant increase in the N 1s photoelectron peak for -NH2 (399 eV). On the TNDDE SAM, XPS showed the -NO2 photoelectron peak again decreasing with prolonged X-ray irradiation whereas no peak was observed at 399 eV; therefore, the -NO2 moieties are selectively cleaved. No change was observed on the other functionalized monolayers apart from the -CO2(t)Bu-functionalized monolayer, where after 100 min of X-ray irradiation approximately 11% of the carbon content was lost. The S 2p and O 1s spectra remained unchanged during the irradiation suggesting the conversion of the -CO2(t)Bu to the -COOH moiety, although the conversion was not complete because the tertiary butyl moiety contributes 25% to the total carbon content of the SAM. Also, there was no evidence of the molecules desorbing from the substrate for any of the SAMs studied during the X-ray irradiation as shown by no change in the S 2p and C 1s XPS spectra taken during the X-ray irradiation.

Magnetic field enhanced nano-tip fabrication for four-probe STM studies.

We present an improved method for fabricating tungsten STM tips for measuring nanoscale objects using a multiprobe STM. The tips were prepared by a 'drop-off' technique combined with a magnetic field. For magnetic field strengths above 150 Oe the electrolyte was seen to rotate about the tungsten anode. This rotation reduced the adhesion of bubbles and led to a reduction in the cone angle of the tips produced. This property is advantageous for multiprobe measurements as it allows one to bring multiple tips into close proximity.

Four-probe electrical characterization of Pt-coated TMV-based nanostructures.

The electrical transport and structural properties of tobacco mosaic virus (TMV)-based nanostructures have been studied. Electroless deposition was used to coat the TMV outer surface with a 13 nm thick homogeneous Pt layer. SEM, TEM and electrical characterization of the obtained nanostructures has been performed. Using four independently controlled scanning tunnelling microscope tips we were able to perform four-point probe resistance measurements on linear virus assemblies and demonstrate the continuous nature of the metallic coating. The measured resistivity values of the virial nanowires exceeded the bulk value by 10-100 times; notwithstanding this the coated structure allowed high current densities, of the order of 10(5)-10(8) A cm(-2). The four-probe technique proved to be useful for analysing the electrical properties of bio-inorganic nanowires.

Ellipsometric study of adsorption on nanopatterned block copolymer substrates.

We report ellipsometrically obtained adsorption isotherms for a carefully chosen test liquid on block copolymer films of Kraton G1650, compared with adsorption isotherms on homogeneous films of the constituent polymers. Standard atomic force microscopy images imply the outer surface of Kraton G1650 is chemically patterned on the nanoscale, but this could instead be a reflection of structure buried beneath a 10 nm layer of the lower energy component. Our test liquid was chosen on the basis that it did not dissolve in either component and in addition that it was nonwetting on the lower energy polymer while forming thick adsorbed films on pure substrates of the higher energy component. Our ellipsometry data for Kraton G1650 rule out the presence of segregation by the lower energy constituent to the outer surface, implying a mixed surface consistent with Cassie's law. We discuss implications of our findings and related work for the outer surface structures of block copolymer films.

Nitrate Leaching in Dryland Agroecosystems as Influenced by Soil and Climate Gradients.

Farmers in dryland agriculture areas of the Central Great Plains have characteristically practiced alternate crop-fallow to stabilize yields. Large amounts of N released soon after sodbreaking and more recent fertilizer additions may have contributed to N movement below crop root zones. Improved water conservation techniques during fallow periods increases the possibility of NO3 leaching below root zones in modern-day crop-fallow systems. Soil topography and water supply may affect leaching potential. This study was conducted to test hypotheses regarding landscape position effects and potential evapotranspiration on depth of water and NO3 penetration. Three sites in eastern Colorado with equal annual precipitation, but with mean potential evapotranspiration varying from 1000 to 1900 mm yr-1 were evaluated. A soil catena was sampled at each site to test landscape effects. All sites were previously managed under tilled wheat-fallow systems for at least 50 yr. All summit (upland) sites had higher water and NO3 contents below their crop root zones than nearby native prairie sites. Soil water and NO3 content of soil profiles to a depth of 9 m were not related to slope position, but there was an inverse relationship between water and NO3 content of soil profiles and potential evapotranspiration. The total NO3 -N in cultivated profiles below the normal root zone of crops varied from 126 kg ha-1 at the northern site to 47 kg ha-1 at the southern site. Although some NO3 leaching had occurred, it did not appear to be a major problem for any particular landscape position or climatic zone on cultivated soils.

Spectrophotometric measurements of haemoglobin saturation and concentration in skin during the tuberculin reaction in normal human subjects.

A non-invasive technique employing light-guide spectrophotometry is described for the measurement of haemoglobin concentration and oxygenation in human skin. Measurements were carried out in the visible wavelength range (500-620 nm) and a series of experiments were carried out in vitro and in vivo in order to calibrate the system. Indices were derived for the measurement of relative haemoglobin concentration and absolute oxygen saturation. The technique was applied to measure the changes in these parameters occurring during the course of the tuberculin reaction in human skin. The results are compared with those from laser Doppler flowmetry and transcutaneous oxygen measurements which were carried out concurrently. Divergence between the intracapillary and tissue oxygenation during the course of the reaction provides evidence for the existence of increased diffusion resistance for oxygen; a model is proposed. The study demonstrates the potential clinical usefulness of light-guide spectrophotometry for the non-invasive investigation of tissue oxygen supply.