Ultrafast mid-infrared spectrochronography of dispersion near molecular absorption bands.

Quantitative characterization of dispersion near molecular resonances is difficult both conceptually and technically, as it requires systematic measurements of rapidly varying dispersion profiles across the edges of molecular absorption bands. Here, we show that this challenge can be confronted with an ultrafast spectrochronography technique that combines time-resolved four-wave mixing and cross-correlation frequency-resolved optical gating. We demonstrate that, with the spectrum of an ultrashort mid-infrared laser probe stretching across the edge of a molecular absorption band, the Wigner chronocyclic maps of such a probe reveal universal signatures of anomalous dispersion and help quantify such dispersion anomalies.

[A mathematical model of hemodynamic processes for distal pulse wave formation].

A mathematical model of the formation of distal arterial pulse wave signal in the blood vessels of the upper limbs was considered. The formation of distal arterial pulse wave is represented as a composition of forward and reverse pulse waves propagating along the human arterial system. The system of formal analogy between pulse waves propagation along the human arterial system and the propagation of electrical oscillations in electrical transmission lines with distributed parameters was proposed. Dependencies of pulse wave propagation along the human arterial system were obtained by solving the one-dimensional Navier-Stokes equations for a few special cases.

[Evaluation of various personality characteristics and professional training of interns--internal medicine specialists]. / Otsenka nekotorykh lichnostnykh kachestv i professional'noi podgotovki internov-terapevtov.

On the basis of the anonymous questionnaires filled by 131 therapeutic interns from 18 higher medical establishments young specialists distinguished certain personality traits which, in their opinions, should be inherent in a doctor; they evaluated training in the institute and during internship and pointed out its shortcomings. The information obtained is important for further improvement of the medical training.

[HPLC determination of plasma/serum homocysteine and cysteine with UV detection and solid-phase extraction on a polymeric sorbent].

Isocratic HPLC determination of plasma/serum homocysteine and cysteine with separation on reversed-phase column and UV detection at 330 nm is proposed. The mobile phase consist of acetonitrile - 0.05 M citrate-phosphate buffer with pH 2.4 - isopropanol (15:85:1, v/v/v). Full separation of cysteine, cysteamine (IS), glutathione and homocysteine was achieved within less than 10 minutes. Reduction of thiols from disulfides was performed by 1,4-dithioerithreitol, and derivatization by with Ellman's reagent [5'5-dithiobis-(2-nitrobenzoic acid)]. After that plasma/serum, containing derivatives of thiols, is cleared and concentrated on cartridge packed with 10 mg of hypercross-linked polystyrene (Purosep-200). Elution from cartridge is made with water-organic solvent (without evaporation and concentration, but without dilution), as well as waterless solvents (with evaporation and concentration). Simplicity, reproducibility in combination with high cleanliness of extracts and sufficient sensitivity (0.4 ng for homocysteine, 2 ng for glutathione and 0.2 ng for cysteine and cysteamine at a signal/noise ratio > 3), make this method suitable for routine clinical application.