Fresnel lens optical fiber tweezers to evaluate the vitality of single algae cells.

Dunaliella salina algae are trapped and studied using dual-fiber optical tweezers based on nano-imprinted Fresnel lenses. Different forms of cyclic motion of living algae inside the optical trap are observed and analyzed. A characteristic periodic motion in the 0-35 Hz frequency region reflects the algal flagella activity and is used to estimate the algal vitality, by photomovement. The trap stiffness and optical forces are measured for the case of a dead algal cell. It is shown that the dual-fiber optical tweezers can be used to study the vitality (or viability) property of single cells, a property that is essential and can be scaled up to other applications, such as sperm analysis for fertility tests.

Excitation of whispering gallery modes with a "point-and-play," fiber-based, optical nano-antenna.

We demonstrate the excitation and detection of whispering gallery modes in optical microresonators using a "point-and-play," fiber-based, optical nano-antenna. The coupling mechanism is based on cavity-enhanced Rayleigh scattering. Collected spectra exhibit Lorentzian dips, Fano shapes, or Lorentzian peaks, with a coupling efficiency around 13%. The spectra are characterized by the coupling gap, polarization, and fiber tip position. The coupling method is simple, low-cost and, most importantly, the Q-factor can be maintained at 108 over a wide coupling range, thereby making it suitable for metrology, sensing, or cavity quantum electrodynamics experiments.

Out-of-equilibrium force measurements of dual-fiber optical tweezers.

Optical trapping of micron-size dielectric particles in a dual-fiber tip configuration is presented. Trap oscillation and suspension flow experiments are performed to investigate the linearity of the optical forces. These measurements are completed by standard methods such as Boltzmann statistics or power spectra evaluation. Strong trapping efficiencies of 0.25 and 1.8 pN·µm-1 have been found in the axial and transverse directions, respectively. The values obtained by the different approaches are in good agreement. The measurements show that the optical trapping potential is harmonic over the experimentally attainable distances, i.e., 2.5 and 0.6 µm in the transverse and axial directions, respectively.

Single and dual fiber nano-tip optical tweezers: trapping and analysis.

An original optical tweezers using one or two chemically etched fiber nano-tips is developed. We demonstrate optical trapping of 1 micrometer polystyrene spheres at optical powers down to 2 mW. Harmonic trap potentials were found in the case of dual fiber tweezers by analyzing the trapped particle position fluctuations. The trap stiffness was deduced using three different models. Consistent values of up to 1 fN/nm were found. The stiffness linearly decreases with decreasing light intensity and increasing fiber tip-to-tip distance.

Transmission and reflection characteristics of metal-coated optical fiber tip pairs.

The optical transmission and reflection in between two metalized optical fiber tips is studied in the optical near-field and far-field domains. In addition to aluminum-coated tips for near-field scanning optical microscopy (NSOM), specifically developed gold-coated fiber tips cut by focused ion beam are investigated. Transverse transmission maps of subwavelength width clearly indicate optical near-field coupling between the tips for short tip distances and become essentially Gaussian-shaped for larger distances in the far-field regime. Moreover, concentric reflection fringes observed for NSOM-type tips illustrate the influence of the receiving fiber tip on the emission pattern of the source tip.

Living cell imaging by far-field fibered interference scanning optical microscopy.

We report on the imaging of biological cells including living neurons by a dedicated fibered interferometric scanning optical microscope. The topography and surface roughness of mouse fibroblasts and hippocampal neurons are clearly revealed. This straightforward far-field technique allows fast, high resolution observation of samples in liquids without lengthy alignment procedures or costly components.

Surface plasmon-mediated far-field emission of laser dye solutions.

Angle-resolved reflection and emission spectra of metal gratings consisting of subwavelength grooves and immersed into rhodamine B and rhodamine 19 solutions are presented. The measured reflection and emission dispersion diagrams reveal the surface plasmon polaritons positions and strong plasmon-mediated emission enhancement, respectively. The same grating could be easily reused for the characterization of different dye molecules.

Y0.9 Er0.1 Al3(BO3)4 thin films prepared by the polymeric precursor method for integrated optics.

This work reports on the optimization of Yo.9 Er0.1 Al3(BO3)4 thin films for integrated optics. The films were deposited on silica and silicon substrates using the spin-coating technique involving solutions previously prepared by the polymeric precursor method. These deposits, 400-800 nm thick, were prepared by a 5-10 multi-layer process and heat treatments at different temperatures from glass transition to crystallization temperature, using heating rates of 2 or 5 degrees C/min. The structural characterizations were performed using grazing incidence X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR). Water and/or hydroxyl contents were also evaluated from FT-IR spectra. Microstructural evolution in term of annealing temperatures was analyzed by high resolution scanning electronic microscopy and atomic force microscopy. Optical transmission spectra were used to determine the refractive index and thickness through the envelope method of the films. Finally, the film guiding and optical properties were studied by m-line spectroscopy. The best film showed a good waveguiding with high light-coupling efficiency close to the theoretical limit.

Luminescent nanoparticle trapping with far-field optical fiber-tip tweezers.

We report stable and reproducible trapping of luminescent dielectric YAG:Ce(3+) nanoparticles with sizes down to 60 nm using far-field dual fiber tip optical tweezers. The particles are synthesized by a specific glycothermal route followed by an original protected annealing step, resulting in significantly enhanced photostability. The tweezers properties are analyzed by studying the trapped particles residual Brownian motion using video or reflected signal records. The trapping potential is harmonic in the transverse direction to the fiber axis, but reveals interference fringes in the axial direction. Large trapping stiffness of 35 and 2 pN µm(-1) W(-1) is measured for a fiber tip-to-tip distance of 3 µm and 300 nm and 60 nm particles, respectively. The forces acting on the nanoparticles are discussed within the dipolar approximation (gradient and scattering force contributions) or exact calculations using the Maxwell Stress Tensor formalism. Prospects for trapping even smaller particles are discussed.