Absence of the Min System Does Not Cause Major Cell Division Defects in Agrobacterium tumefaciens.

In A. tumefaciens, the essential FtsZ protein is located at the growth pole before shifting to the mid-cell right before division. Loss of FtsZ causes a halt in cell separation and lysis of cells. To understand how FtsZ polymerization is regulated to properly localize the FtsZ ring at the mid-cell, we have conducted a systematic characterization of the Min system in A. tumefaciens. Our findings indicate that the Min system is not required for cell survival. Yet, we find that the deletion of either minE or minCDE results in a broad cell size distribution, including an increase in the proportion of short and long cells. We observe that the site of constriction is misplaced in the minE or minCDE deletion strains allowing for short cells to arise from sites of constriction near the cell poles. Remarkably, the short cells are viable and contain DNA. In order to observe chromosome replication and segregation in these strains, YFP-ParB is used as a proxy to track the origin of replication as cells elongate and divide. In the absence of the Min proteins, duplication and segregation of the origin of replication is frequently delayed. Taken together, our data suggest that the Min system contributes to the proper regulation of FtsZ placement and subsequent cell division. Furthermore, the failure to precisely place FtsZ rings at mid-cell in the min mutants impacts other cell cycle features including chromosome segregation.

Implementing computerized provider order entry with an existing clinical information system.

BACKGROUND: There are numerous barriers to successfully implementing computerized provider order entry (CPOE), and it is not entirely clear to what degree the proposed benefits extend to older, commercially available systems in place at most hospitals. METHODS: In 2000, Loyola University Health System leadership chartered a project to implement CPOE for hospitalized patients' medications. The impact of CPOE on workflow was analyzed before implementation. Hardware availability was ensured and input screens were customized for users when possible. A formal education and communication plan was implemented to help reduce resistance to change. RESULTS: Full implementation took 20 months. Transcription-related errors per month decreased by 97% from 72.4 to 2.2 per month. During the pilot period, prescribing-related errors increased by 22% from 150 per month to 184 per month-and subsequently decreased to an average of 80 per month, a 47% reduction compared with the baseline error rate. Pharmacist time saved was estimated at 23 hours per month. DISCUSSION: Using an existing CPOE system can provide an affordable, intermediate step on the journey toward implementing a new, state-of-the-art system that provides advanced clinical decision support.