RESUMEN
Deletion of the biosynthetic 4,6-dehydratase gene, jadT, present in the angucycline jadomycin dideoxysugar biosynthetic pathway, led to the isolation of a novel C12 glucosylated jadomycin. JadS was identified as the catalyst responsible for glucosylation due to a loss of production of the glucosylated natural product in a ΔjadSΔjadT deletion strain. This study demonstrates that a 2,6-dideoxy-l-sugar glycosyltransferase is able to transfer d-glucose, exemplifying remarkable substrate tolerance.
Asunto(s)
Productos Biológicos/metabolismo , Glicosiltransferasas/metabolismo , Hidroliasas/genética , Isoquinolinas/metabolismo , Productos Biológicos/química , Productos Biológicos/aislamiento & purificación , Eliminación de Gen , Glicosilación , Hidroliasas/metabolismo , Isoquinolinas/química , Isoquinolinas/aislamiento & purificación , Conformación Molecular , Especificidad por SustratoRESUMEN
Cps2L, a thymidylytransferase, is the first enzyme in Streptococcus pneumoniae L-rhamnose biosynthesis and an antibacterial target. We herein report the evaluation of six sugar phosphate analogues selected to further probe Cps2L substrate tolerance. A modified continuous spectrophotometric assay was employed for facile detection of pyrophosphate (PPi) released from nucleotidylyltransfase-catalysed condensation of sugar 1-phosphates and nucleoside triphosphates to produce sugar nucleotides. Additionally, experiments using waterLOGSY NMR spectroscopy were investigated as a complimentary method to evaluate binding affinity to Cps2L.