RESUMEN
OBJECTIVE: To present the first reported case of synchronous sperm retrieval followed by sperm washing before an intracytoplasmic sperm injection (ICSI) cycle in an HIV-positive azoospermic man. DESIGN: Case report. SETTING: Assisted reproduction center. PATIENT(S): A 40-year-old HIV-positive man with obstructive azoospermia due to vasal aplasia. INTERVENTION(S): Synchronous sperm retrieval, sperm washing, nucleic acid-based sequence amplification testing, and intracytoplasmic sperm injection. MAIN OUTCOME MEASURE(S): Successful sperm retrieval sufficient for sperm washing and fertilization. RESULT(S): Sufficient quantity of spermatozoa for washing was obtained at epididymal aspiration. After the wash, HIV ribonucleic acid (RNA) was undetectable with nucleic acid-based sequence amplification testing, enabling injection of oocytes collected after routine gonadotropin superovulation. Of seven oocytes collected from the 39-year-old woman partner, six were injected and five fertilized (83%). Three embryos were transferred on day 2. The pregnancy test was negative on this occasion. CONCLUSION(S): This case demonstrates that sperm washing can be applied in cases of sperm retrieval where sperm volume and density is low, allowing the treatment of azoospermic HIV-positive men.
Asunto(s)
Seropositividad para VIH , Oligospermia/virología , Inyecciones de Esperma Intracitoplasmáticas , Espermatozoides , Irrigación Terapéutica , Recolección de Tejidos y Órganos , Adulto , Femenino , VIH/genética , Seropositividad para VIH/virología , Humanos , Masculino , ARN Viral/análisis , Espermatozoides/virología , Factores de Tiempo , Conducto Deferente/anomalíasAsunto(s)
Dispareunia/diagnóstico , Adolescente , Adulto , Anciano , Dispareunia/psicología , Dispareunia/terapia , Femenino , Humanos , Anamnesis , Persona de Mediana Edad , Examen Físico , Adulto JovenRESUMEN
OBJECTIVE: To collect data on the demand and provision of fertility care in HIV-infected couples in the United Kingdom and data on the etiology of subfertility in this population. DESIGN: A postal questionnaire survey and audit of causes of infertility in HIV-infected women. SETTING: Seventy-four Human Embryology and Fertilisation Authority-registered assisted conception units (ACUs) and 294 genitourinary medicine (GUM) clinics in the United Kingdom were sent questionnaires. PATIENT(S): Sixty-five HIV-infected women attending the Research Clinic at the Chelsea and Westminster ACU. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Number of ACUs treating HIV-infected patients and number of GUM clinics receiving requests for referral, as well as the etiology of subfertility in HIV-infected women attending our clinic. RESULT(S): Response rates from ACUs and GUM clinics were 93% and 63%, respectively. Fourteen ACUs (20%) were treating HIV-infected men; of these, seven (10%) performed sperm washing, but only two (3%) tested sperm for HIV after processing, before use. Nine units (13%) treated HIV-infected women, but only three ACUs (4%) had separate laboratories for handling potentially infected gametes or embryos. Of the 15,211 patients registered in 81 GUM clinics, 4% of the men and 16% of the women had requested advice on conceiving. An audit of the Chelsea and Westminster HIV fertility clinic demonstrated a 40% prevalence of tubal factor infertility in HIV-infected women. CONCLUSION(S): Demand is high, and set to increase, but current suboptimal practice in some centers is placing unaffected partners and the unborn child at risk of seroconversion.