Extra-phosphoric effects of phytase with and without xylanase in corn-soybean meal-based diets fed to broilers.

Two experiments were conducted to evaluate the extra-phosphoric effects of phytase on amino acid (AA) and energy digestibility (experiments 1 and 2) and growth performance (experiment 2) of broilers fed diets adequate in Ca and nonphytate P supplemented with xylanase. Ross × Ross 708 broiler chicks (864 males in experiment 1 and 1,152 females in experiment 2) were randomly distributed into battery cages (6 replicate cages per treatment) with 12 birds per cage at 1 d of age. In both experiments, factorial arrangements of treatments were evaluated consisting of 6 phytase [0, 1,000, 2,000, 4,000, 8,000, or 16,000 phytase units (FTU)/kg] and 2 xylanase [0 or 16,000 birch xylan units (BXU)/kg] concentrations in experiment 1 and 4 phytase (0, 500, 1,000 or 2,000 FTU/kg) and 4 xylanase (0, 8,000, 16,000, or 32,000 BXU/kg) concentrations in experiment 2. Treatments were provided from 27 to 32 d of age in experiment 1 and from 1 to 32 d of age in experiment 2. Digesta contents of the terminal ileum were collected at 32 d of age (experiment 1 and 2), and growth performance was measured at 1, 14, and 25 d of age in experiment 2. There was no interaction of phytase and xylanase; only main effects of phytase were observed. In experiment 1, broilers fed diets supplemented with phytase at 1,000 FTU/kg had increased (P < 0.05) apparent ileal digestibility (AID) of all AA with the exception of Ala and Met. Diets fed to broilers supplemented with higher concentrations of phytase did not further increase AID of any AA (P > 0.05) above the addition of 1,000 FTU/kg of phytase. Phytase supplementation did not affect ileal digestible energy (P > 0.05). For all variables measured, significant log-linear or log-quadratic effects of phytase (P > 0.05) were not observed. In contrast, broilers fed diets supplemented with phytase in experiment 2 exhibited log-linear (P < 0.05) increases in AID of AA but not apparent ileal digestible energy. However, supplementation with 2,000 FTU/kg of phytase increased (P = 0.05) ileal digestible energy by 36 kcal/kg compared with the basal diet. Broilers fed diets with 1,000 FTU/kg of phytase had improved growth performance (P < 0.05) in comparison with broilers fed diets containing 0 or 500 FTU/kg of phytase. The addition of 500 FTU/kg of phytase in diets fed to broilers did not affect ileal digestibility or growth performance (P > 0.05). Data obtained from these experiments demonstrated extra-phosphoric effects in broilers fed diets supplemented with 1,000 FTU/kg of phytase and diminishing returns with higher concentrations.

Interactive effects of phytase and xylanase supplementation with extractable salt-soluble protein content of corn in diets with adequate calcium and nonphytate phosphorus fed to broilers.

The objective was to determine the effects of extractable salt-soluble protein content of corn (PS) and exogenous enzyme supplementation on N, starch, and energy digestibility in broilers fed diets adequate in Ca and nonphytate P. Broilers were randomly distributed into floor pens (6 replicate pens per treatment) with 28 birds per pen at 1 d of age. Treatments consisting of 4 sources of corn varying in PS (A, 58.1; B, 54.2; C, 53.7; and D, 30.6 mg of BSA equivalent values) with or without phytase (0 and 1,000 phytase units/kg) and xylanase (0 and 16,000 units of xylanase activity/kg) were randomly assigned to each pen. Different sources of corn were provided from 1 to 9 and 24 to 29 d of age. However, enzyme treatments were provided throughout the experiment. From 1 to 9 d of age, no interactions were observed. Apparent ileal N digestibility (AIND) and apparent ileal digestible energy (IDE) of diets with the lowest PS (based on corn D) were lower (P ≤ 0.05) than those of diets with a higher PS. Phytase increased (P ≤ 0.01) AIND and IDE by 5 and 16%, respectively, and xylanase exerted the opposite effect (P ≤ 0.03). From 24 to 29 d of age, phytase and xylanase in combination resulted in reduced (P ≤ 0.05) AIND of diets with a low PS (based on corn D) compared with the basal diet in broilers. Broilers fed diets with the highest or lowest PS (based on corn A or D) had lower (3-way interaction; P ≤ 0.05) IDE when phytase and xylanase were supplemented in combination compared with either enzyme alone. In conclusion, responses to exogenous enzyme supplementation are not constant and are influenced by the source of ingredients as well as the age of broilers. The magnitudes of the responses to phytase on nutrient and energy digestibility were greater at 9 compared with 29 d of age.

Effects of corn source on the relationship between in vitro assays and ileal nutrient digestibility.

An experiment was conducted to determine the relationship between in vitro assays to estimate quality and ileal nutrient and energy digestibility of various corn sources. Twelve samples of corn were analyzed for gross energy (GE), N, moisture, crude fat (CF), salt-soluble protein content (SSP), and vitreousness. Six of the 12 sources of corn had similar proximate composition but ranged in pairs as having low, moderate, or high quality based on protein solubility that varied from 25.7 to 49.2%. Experimental diets consisted of corn sources with 0.50% TiO2. In total, 504 (12 per pen; 0.039 m2 per bird) Ross×Ross 708 male broiler chicks were randomly distributed to 42 pens (7 replicates per treatment) at 1 d of age. Broilers were fed common starter and grower diets from 1 to 27 d of age and experimental diets from 28 to 30 d of age. At 30 d of age, 8 birds per cage were euthanized for digesta collection from 4 to 30 cm proximal to the ileocecal junction. Feed and digesta were analyzed for TiO2, GE, N, CF, and starch content. Ileal digestibility of N (apparent), CF, and starch did not differ (P>0.05) among sources of corn. Apparent ileal digestible energy (IDE) of the 6 corns averaged 3,323 kcal/kg. Salt-soluble protein concentration was correlated with IDE among the corns (r=0.5; P<0.001). Ileal N and fat digestibility were correlated with IDE (r=0.4 and 0.3, respectively; P<0.05). Apparent MEn ranged from 3,262 to 3,342 kcal/kg and was correlated with SSP (r=0.8; P<0.001) and IDE (r=0.36; P<0.05). These results indicated that sources of corn with similar proximate composition may vary in their digestible energy content, and in such a situation, SSP may be used to differentiate those with wide-ranging IDE or AMEn. However, further research is required to investigate the relationship between SSP and growth performance of broilers.

Apparent metabolizable energy needs of male and female broilers from 36 to 47 days of age.

Two experiments were conducted to examine AME(n) responses of Ross × Ross 708 male and female broilers from 36 to 47 d of age. In each experiment, 1,440 male and female broilers were randomly distributed into 96 floor pens (15 birds/pen; 8 replicate pens/treatment) sexed separately and were fed 6 levels of AME(n) ranging from 3,140 to 3,240 kcal/kg in increments of 20 kcal of AME(n)/kg, resulting in a 6 × 2 factorial arrangement of treatments. Body weight gain, feed intake, feed conversion, AME(n) intake, AME(n) intake per BW gain, plasma 5-triiodothyronine and thyroxine concentrations, mortality, and meat yields were evaluated during experimentation. Average temperature and RH were 20.3°C and 49.0% for experiment 1 and 26.1°C and 66.6% for experiment 2. In experiment 1, broilers fed progressive additions of AME(n) had lower (P ≤ 0.02) feed intake, feed conversion, and caloric conversion. Progressive increments of AME(n) did not influence plasma 5-triiodothyronine and thyroxine concentrations, carcass yield, or breast meat yield. No AME(n) × sex interactions were observed in experiment 1. In experiment 2, AME(n) × sex linear interactions (P ≤ 0.04) were observed for BW gain, caloric conversion, carcass weight, and total breast weight. Male broilers responded more to higher AME(n) levels than did female broilers. These data indicate that AME(n) needed for growth performance of broilers from 36 to 47 d of age differed between experiments.