Yeast derivatives and wheat germ in the adult diet modulates fecundity in a tephritid pest.

Anastrepha fraterculus (Wiedemann), a pest of great economic importance in South America, needs urgently to be controlled by environmentally friendly methods such as the sterile insect technique for which mass rearing of insects is required. Because oogenesis takes place during the adult stage, mass-rearing facilities should provide the females a diet that maximizes egg production at the lowest cost. Accordingly, we investigated the effect of artificial protein sources in the adult diet (yeast derivatives of different cost but with similar amino acids profiles, and the addition of wheat germ) on fecundity. Additionally, we evaluated different ratios of yeast derivatives or wheat germ on ovary maturation, fecundity, and fertility as well as their association with the nutrient content of females. Females fed hydrolyzed yeast and yeast extract attained the highest fecundity level, and those fed brewer's yeast the lowest. Reducing the amount of hydrolyzed yeast, an expensive protein source, in the diet negatively affected fecundity and ovary maturation. Increasing the amount of brewer's yeast, a low-cost protein source, did not favor fecundity. The addition of wheat germ in the adult diet improved fecundity regardless of the yeast derivate considered. Percentage of egg hatch was not affected by the diet. Nutrient content of A. fraterculus females varied according to the adult diet provided and mating status. Our findings provide novel baseline information to understand the role of nutrition on reproductive performance of A. fraterculus females and are discussed in the context of resource allocation. They also provide valuable advances in the search for cost-effective adult diets at fruit fly mass rearing facilities.

Impact of natural control agents of the citrus leafminer Phyllocnistis citrella on lemon trees varies among seasons.

Studies on insect natural enemies and their effects on host populations are of immense practical value in pest management. Predation and parasitism on a citrus pest, the leafminer Phyllocnistis citrella Stainton, were evaluated by sampling over 3 years in four locations within a world leading lemon producing area in Northwest Argentina. Both mortality factors showed seasonal trends consistent across locations, with predation exerting earlier and more sustained pressure than parasitism, which showed wider seasonal variations. The dominant parasitoids, native Cirrospilus neotropicus and introduced Ageniaspis citricola, showed different seasonal trends: C. neotropicus was dominant in spring whereas A. citricola superseded it in autumn and winter. Although parasitism rates were relatively low, the native C. neotropicus revealed favourable features as potential control agent, by showing density-dependence, parasitism rates comparable with those of the specific A. citricola during part of the cycle, and earlier synchronization with the host. The study provides highly relevant information for a sustainable management of this worldwide pest, for which biological control is considered the best long-term option.

Spatial and temporal variation in Chaetanaphothrips orchidii Moulton (Thysanoptera: Thripidae) population and its damage on lemon.

Chaetanaphothrips orchidii Moulton has recently been detected in lemon (Citrus limon) orchards in northwest Argentina, causing high levels of damage on fruits. Severe damage results in the rejection of fruit for export, which must then be sold in the industry. However, the restrictions imposed by the citrus industry on insecticide residues sometimes also result in fruit rejection. Here, we studied the ecology and behavior of C. orchidii in order to propose a pest management strategy that could meet both export and industry demands. Seasonal occurrence and canopy distribution of C. orchidii in lemon orchards were evaluated, and field experimental manipulations of thrips populations were performed to analyze how the length (45, 100, 130, and 200 days) and timing (January, February, or March) of C. orchidii activity related with fruit damage. Lemons harvested during summer showed lower infestation levels (∼0.64 individual per fruit) than those harvested in winter (∼1.88 individuals per fruit). Higher proportions of damaged fruits were recorded in the lower part of the tree. Changes in the population levels of C. orchidii were closely associated with fruit phenology. The longer the lemon fruits were exposed to the thrips, the higher was the damage. However, the time of infestations did not affect fruit damage. Our data provide a first step towards understanding the factors that determine the severity of fruit damage caused by C. orchidii in northwest Argentina.