Lacticaseibacillus parahuelsenbergensis sp. nov., Lacticaseibacillus styriensis sp. nov. and Lacticaseibacillus zeae subsp. silagei subsp. nov., isolated from different grass and corn silage.

Four rod-shaped, non-motile, non-spore-forming, facultative anaerobic, Gram-stain-positive lactic acid bacteria, designated as EB0058T, SCR0080, LD0937T and SCR0063T, were isolated from different corn and grass silage samples. The isolated strains were characterized using a polyphasic approach and EB0058T and SCR0080 were identified as Lacticaseibacillus zeae by 16S rRNA gene sequence analysis. Based on whole-genome sequence-based characterization, EB0058T and SCR0080 were separated into a distinct clade from Lacticaseibacillus zeae DSM 20178T, together with CECT9104 and UD2202, whose genomic sequences are available from NCBI GenBank. The average nucleotide identity (ANI) values within the new subgroup are 99.9 % and the digital DNA-DNA hybridization (dDDH) values are 99.3-99.9 %, respectively. In contrast, comparison of the new subgroup with publicly available genomic sequences of L. zeae strains, including the type strain DSM 20178T, revealed dDDH values of 70.2-72.5 % and ANI values of 96.2-96.6 %. Based on their chemotaxonomic, phenotypic and phylogenetic characteristics, EB0058T and SCR0080 represent a new subspecies of L. zeae. The name Lacticaseibacillus zeae subsp. silagei subsp. nov. is proposed with the type strain EB0058T (=DSM 116376T=NCIMB 15474T). According to the results of 16S rRNA gene sequencing, LD0937T and SCR0063T are members of the Lacticaseibacillus group. The dDDH value between the isolates LD0937T and SCR0063T was 67.6 %, which is below the species threshold of 70 %, clearly showing that these two isolates belong to different species. For both strains, whole genome-sequencing revealed that the closest relatives within the Lacticaseibacillus group were Lacticaseibacillus huelsenbergensis DSM 115425 (dDDH 66.5 and 65.9 %) and Lacticaseibacillus casei DSM 20011T (dDDH 64.1 and 64.9 %). Based on the genomic, chemotaxonomic and morphological data obtained in this study, two novel species, Lacticaseibacillus parahuelsenbergensis sp. nov. and Lacticaseibacillus styriensis sp. nov. are proposed and the type strains are LD0937T (=DSM 116105T=NCIMB 15471T) and SCR0063T (=DSM 116297T=NCIMB 15473T), respectively.

Deconvolution of 1D NMR spectra: A deep learning-based approach.

The analysis of nuclear magnetic resonance (NMR) spectra to detect peaks and characterize their parameters, often referred to as deconvolution, is a crucial step in the quantification, elucidation, and verification of the structure of molecular systems. However, deconvolution of 1D NMR spectra is a challenge for both experts and machines. We propose a robust, expert-level quality deep learning-based deconvolution algorithm for 1D experimental NMR spectra. The algorithm is based on a neural network trained on synthetic spectra. Our customized pre-processing and labeling of the synthetic spectra enable the estimation of critical peak parameters. Furthermore, the neural network model transfers well to the experimental spectra and demonstrates low fitting errors and sparse peak lists in challenging scenarios such as crowded, high dynamic range, shoulder peak regions as well as broad peaks. We demonstrate in challenging spectra that the proposed algorithm is superior to expert results.

[Prophylactic effect of isosorbide dinitrate on postoperative cardiac complications (author's transl)]. / Prophylaktische Wirkung von Isosorbiddinitrat auf postoperative kardiale Komplikationen

Isosorbide dinitrate (ISD) was administered before, during and after 178 operations performed on 127 patients with arterial occlusive disease. Its influence on postoperative myocardial infarction, heart failure and mortality was tested by comparison with 188 operations performed on 140 patients with hypertension and/or old myocardial infarction receiving no ISD prophylaxis. Risk of cardiac complications was similar in both groups. Mortality in the ISD-treated group was significantly lowered as compared with the control group and was about half of the overall mortality in patients with arterial occlusive disease operated on at our hospital over the past 10 years. This difference depended partly on the influence of ISD on cardiac complications. Post-operative myocardial infarction during ISD prophylaxis occurred in 0.6% of cases as compared with 3.7% in the control group (p less than 0.05), whilst the respective values for postoperative heart failure were 5.7% and 18.2% (p less than 0.001). Both complications are related to absolute or relative hypoxia during the post-operative stress period. ISD is effective by lowering cardiac preload and afterload and thereby diminishing myocardial oxygen demand. ISD is the drug of choice for surgical patients since it provides a steady and long-lasting effect after sublingual absorption. ISD prophylaxis during the perioperative period is indicated in cases with coronary artery disease and with increased cardiac preload or afterload.

[The implementation of a computerized documentation system for the evaluation of spa therapy in patients with rheumatoid arthritis (author's transl)]. / Der Einsatz einer EDVA in der Erfolgsbeurteilung von Kuren bei Patienten mit chronischer Polyarthritis

A previously-described documentation system for articular examination was applied to evaluate the results of spa treatment in 20 patients with rheumatoid arthritis during 3 separate treatment periods. Physical joint findings were documented and indices were compiled by means of a computer before, during and after treatment with thermal water the first year, normal water the second year (or vice versa) and without baths at all in the third year. These indices were compared statistically. Each kind of treatment produced a statistically-significant improvement in the disease, there being no significant difference in the results achieved by the 3 therapeutic regimens.

[Documentation of obstetric case reports by means of video-terminals as a part of the Vienna General medical information system "WAMIS" (author's transl)]. / Die Dokumentation geburtshilflicher Krankengeschichten mit Hilfe von Bildschirmterminals im Rahmen des Informationssystems WAMIS

A report is given on the introduction of a complete documentation system of obstetric case reports by means of video-terminals and printer-terminals in both departments of Obstetrics and Gynaecology of the 1niversity of Vienna. The special routine for the admission of patients, which produces the heading of the case report is demonstrated, as well as the sheets of collecting the data in respect to case history, antenatal examinations, labour room reports and details of obstetric operations. The computer prints the case history using the collected data. These computer-printed case reports replace the customary handwritten reports. Laboratory data, therapeutic measures and final diagnosis are computed in the same way. The advantages of this form of organization of data collection for clinical purposes and research work are discussed.

[A retrospective study of carcinomas of the oral cavity: a preliminary report (author's transl)]. / Karzinome der Mundhöhle. Vorläufiger Bericht über eine retrospektive Studie.

UNLABELLED: The German-Austrian-Swiss Research Team on Maxillofacial Tumours is investigating a series of 585 carcinomas of the oral cavity, lips and oropharynx by biostatistical methods. The aim of the study is the determination of relevant prognostic symptoms of oral cavity carcinomas, classification of the series on the basis of these symptoms, the determination of therapeutic efficacy and the assessment of collective and individual prognosis. PRELIMINARY RESULTS: 1. Factors of prognostic relevance are probably only tumour size and intensity of regional metastases. 2. The classification of the series becomes problematic due to the lack of relevant prognostic factors.

Keep breathing! Common motion helps multi-modal mapping.

We propose an unconventional approach for transferring of information between multi-modal images. It exploits the temporal commonality of multi-modal images acquired from the same organ during free-breathing. Strikingly there is no need for capturing the same region by the modalities. The method is based on extracting a low-dimensional description of the image sequences, selecting the common cause signal (breathing) for both modalities and finding the most similar sub-sequences for predicting image feature location. The approach was evaluated for 3 volunteers on sequences of 2D MRI and 2D US images of the liver acquired at different locations. Simultaneous acquisition of these images allowed for quantitative evaluation (predicted versus ground truth MRI feature locations). The best performance was achieved with signal extraction by slow feature analysis resulting in an average error of 2.6 mm (4.2 mm) for sequences acquired at the same (a different) time.

[The Vienna General Medical Information System (WAMIS)]. / Wiener Allgemeines Medizinsches Informationssystem (WAMIS)

The Vienna General Medical Information System is applied at the university clinics of the medical faculty of Vienna. The system consists of several components: 1. Program complex for the support of daily routine work, 2. documentation system, 3. Vienna laboratory system, 4. differential diagnosis and screening system, 5. medical information system, 6. medical evaluation system. The central part of the system is the patient-orientated data bank.

Approach to a hospital-based application of a medical expert system.

CADIAG-2 is a data-driven fuzzy medical expert system built for computer-based consultation in internal medicine. It has been integrated into the medical information system WAMIS of the Vienna General Hospital. Through the integration, CADIAG-2 is able to access patient data and laboratory test results already collected in the central patient database of WAMIS. CADIAG-2 operates in two subsequent phases: (1) as an automatic screening procedure for detecting pathological states in the patient, for generating diagnostic hypotheses, and for proposing further useful examinations; and (2) as an on-line consultation system for the clinician to assist him in clarifying patients' disorders completely and in great detail. At present, CADIAG-2 is subject to extended clinical trials. There are four application areas: rheumatic diseases, pancreatic diseases, gall bladder and bile duct diseases and colon diseases. First results on the performance of CADIAG-2 based on the evaluation of about 500 cases were obtained. In this paper, the principal goals, main components and concepts of CADIAG-2, and the experience gained until now are discussed.

[Therapy evaluation in rheumatology with an electronic data processing system]. / Therapiebeurteilung in der Rheumatologie mit einem elektronischen Datenverarbeitungssystem

A system for the evaluation of therapeutical effects in rheumatic diseases is presented. This system permits a calculation of two indices: i.e. rheuma-number and "Masszahl" (= only measured values such as range of motion in degrees) which lend themselves to statistical treatment. The result of standardized physical joint examination of patients is recorded on two mark-pages, specially developed for documentation of joint status. The data recorded on the two-mark-pages are then entered into the computer by means of a mark-page-reader. On the basis of these data, the computer, upon the basis of a program specially developed for this purpose, automatically calculates the corresponding amount of negative-points, which parallels the severity of the joint changes, i.e. the rheuma-number ("Rheumazahl"). Separately listed at the end of the status-outprint is the "Masszahl", which is part of the rheuma-number and comprises the measured values (for example, the range of motion measured in degrees). The practicability of this system is demonstrated on the basis of the results of a double-blind-comparison of two antirheumatic-corticosteroid-combinations and an open study with immuno-suppressives in patinets with rheumatoid arthritis.

[Risk factors in postoperative myocardial infarction]. / Risikofaktoren des postoperativen Myokardinfarktes

Myocardial infarction after major surgery occurred 25 times in 214 patients who had previously sustained infarcts. Analysis of data obtained before, during and after 335 operations in these patients revealed the following pathogenetic factors in the infarction: (1) The patient with the highest coronary risk had arterial hypertension of at least 160/95 mmHg and advanced arteriosclerosis combined with coronary arterial, peripheral arterial, cerebrovascular, and renovascular disease. (2) Myocardial necrosis occurred when oxygen supply was reduced, as evidenced from a fall in systolic blood pressure to 70 mmHg or less during operation or anaemia (RBC smaller than or equal to 3,5 times 10-6/mul) early after operation. (3) Risk of infarction was highest during the early postoperative stress period with elevated plasma catecholamine levels and thus an increased myocardial oxygen demand.

[X-ray documentation of the anamnesis and physical data in patients with rheumatism within the scope of the medical information system WAMIS]. / Bildschirmdokumentation von Anamnese und physikalischem Befund bei Rheumakranken Im Rahmen des medizinischen Informationssystems WAMIS

A computerized on-line system of evaluating of symptoms and physical data in rheumatology is presented. Case history, previous treatment, and physical examination are documented. The documentation of the latter may be performed in two ways: one records only joint symptoms, the other includes joint measurements. In comparison to the former off-line method, all data are much better available. Moreover, we believe it to be considerably timesaving. This system should also allow an electronic differential diagnosis of rheumatic diseases, after completing laboratry and X-ray documentation.

[Diagnostic key for intensive care patients: combination of clinical parameters with laboratory findings]. / Diagnoseschlüssel für Intensivpatienten: Kombination klinischer Befunde mit Laboratoriumsbefunden.

A new diagnostic key has been established based on the reports of 3845 critically ill patients in our medical intensive care unit. The clinical diagnoses in these patients were classified in 22 different groups according to different organs or etiological entities (diseases of the liver, infectious diseases, intoxications etc.). 42 different laboratory parameters were selected for classification of metabolic or organ-related complications. Combining of clinical diagnoses with laboratory values characterizes the seriously ill patient. Our new system proved to be practicable in describing the degree, complications and prognosis of disorders in intensive care medicine. The results are demonstrated in patients with hepatic coma.

[Use of the computer in clinical medicine]. / Computereinsatz in der klinischen Medizin.

This article describes the application of computers in clinical medicine and the experience gained by the Institute of Medical Computer Science when introducing computer systems into the clinics of the University of Vienna Medical School in the last 20 years. It is shown what dramatic development has taken place in these years. The medical information system WAMIS with its central patient database is described as well as the medical record keeping documentation and retrieval system WAREL, which is destined to analyze medical natural language data. A further chapter deals with computers in clinical laboratories. At the end it is tried to point out future trends in applying computers in clinical medicine.