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1.
Nature ; 535(7612): 367-75, 2016 07 21.
Artículo en Inglés | MEDLINE | ID: mdl-27409810

RESUMEN

The transcriptional underpinnings of brain development remain poorly understood, particularly in humans and closely related non-human primates. We describe a high-resolution transcriptional atlas of rhesus monkey (Macaca mulatta) brain development that combines dense temporal sampling of prenatal and postnatal periods with fine anatomical division of cortical and subcortical regions associated with human neuropsychiatric disease. Gene expression changes more rapidly before birth, both in progenitor cells and maturing neurons. Cortical layers and areas acquire adult-like molecular profiles surprisingly late in postnatal development. Disparate cell populations exhibit distinct developmental timing of gene expression, but also unexpected synchrony of processes underlying neural circuit construction including cell projection and adhesion. Candidate risk genes for neurodevelopmental disorders including primary microcephaly, autism spectrum disorder, intellectual disability, and schizophrenia show disease-specific spatiotemporal enrichment within developing neocortex. Human developmental expression trajectories are more similar to monkey than rodent, although approximately 9% of genes show human-specific regulation with evidence for prolonged maturation or neoteny compared to monkey.


Asunto(s)
Encéfalo/crecimiento & desarrollo , Encéfalo/metabolismo , Macaca mulatta/genética , Transcriptoma , Envejecimiento/genética , Animales , Trastorno del Espectro Autista/genética , Encéfalo/citología , Encéfalo/embriología , Adhesión Celular , Secuencia Conservada , Femenino , Humanos , Discapacidad Intelectual/genética , Masculino , Microcefalia/genética , Neocórtex/embriología , Neocórtex/crecimiento & desarrollo , Neocórtex/metabolismo , Trastornos del Neurodesarrollo/genética , Neurogénesis/genética , Factores de Riesgo , Esquizofrenia/genética , Análisis Espacio-Temporal , Especificidad de la Especie , Transcripción Genética/genética
2.
Cell Stem Cell ; 20(1): 120-134, 2017 01 05.
Artículo en Inglés | MEDLINE | ID: mdl-28094016

RESUMEN

During human brain development, multiple signaling pathways generate diverse cell types with varied regional identities. Here, we integrate single-cell RNA sequencing and clonal analyses to reveal lineage trees and molecular signals underlying early forebrain and mid/hindbrain cell differentiation from human embryonic stem cells (hESCs). Clustering single-cell transcriptomic data identified 41 distinct populations of progenitor, neuronal, and non-neural cells across our differentiation time course. Comparisons with primary mouse and human gene expression data demonstrated rostral and caudal progenitor and neuronal identities from early brain development. Bayesian analyses inferred a unified cell-type lineage tree that bifurcates between cortical and mid/hindbrain cell types. Two methods of clonal analyses confirmed these findings and further revealed the importance of Wnt/ß-catenin signaling in controlling this lineage decision. Together, these findings provide a rich transcriptome-based lineage map for studying human brain development and modeling developmental disorders.


Asunto(s)
Encéfalo/embriología , Linaje de la Célula , Desarrollo Embrionario , Células Madre Embrionarias Humanas/citología , Análisis de la Célula Individual/métodos , Animales , Encéfalo/metabolismo , Línea Celular , Linaje de la Célula/genética , Células Clonales , Desarrollo Embrionario/genética , Humanos , Ratones , Modelos Biológicos , Neuronas/citología , Neuronas/metabolismo , Reproducibilidad de los Resultados , Análisis de Secuencia de ARN , Factores de Transcripción/metabolismo , Transcriptoma/genética , Vía de Señalización Wnt/genética
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