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Inside sperm flagella, there are nine doublet microtubules composed of A and B tubules. In this issue of Cell, Leung et al. and Zhou et al. present high-resolution cryo-EM structures of doublet microtubules from mammalian sperms and show unprecedented structures of the A tubules, which are almost entirely occupied with tektin bundles.
Asunto(s)
Microtúbulos , Semen , Animales , Masculino , Microtúbulos/química , Proteínas de Microtúbulos/química , Cola del Espermatozoide/química , Flagelos , MamíferosRESUMEN
The dynamical evolution of light in asymmetric microcavities is of primary interest for broadband optical coupling and enhanced light-matter interaction. Here, we propose and demonstrate that the chaos-assisted photon transport can be engineered by regular periodic orbits in the momentum-position phase space of an asymmetric microcavity. Remarkably, light at different initial states experiences different evolution pathways, following either regular-chaotic channels or pure chaotic channels. Experimentally, we develop a nanofiber technique to accurately control the excitation position of light in the phase space. We find that the coupling to high-Q whispering gallery modes depends strongly on excitation in islands or chaotic sea, showing a good agreement with the theoretical prediction. The engineered chaotic photon transport has potential in light manipulation, broadband photonic devices, and phase-space reconstruction.
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We propose and demonstrate the localization of resonant modes in a Limaçon optical microcavity with layered phase space involving both major and minor partial barriers. By regulating the openness of the cavity through the refractive index control, the minor partial barriers, which do not directly confine the long-lived resonant modes, are submerged successively into the leaky region. During the invalidation process of the minor partial barriers, it is found that the quality factor and the conjugate momentum of the resonant modes exhibit changes with the emergence of turning points. Such phenomena are attributed to the joint confinement effect by the minor partial barriers together with the major one in the layered phase space. This paper helps to improve the understanding of complex dynamics, and sheds light on the fine design of photonic devices with high performance.
RESUMEN
OBJECTIVE: The aim of the study was to investigate the role of microRNA-21 (miR-21) in cardiomyocyte apoptosis and to determine a possible mechanism. METHODS: H9c2 embryonic rat heart-derived cells were used in the study. Cell viability was determined using the 3-(4.5-dimethyl-2-thiazolyl)-2,5- diphenyl-2-H-tetrazolium bromide (MTT) assay, and flow cytometry was used to evaluate cell apoptosis. Reverse transcription-polymerase chain reaction and western blot assays were used to detect mRNA and protein expression of the apoptosis-related proteins and miR-21. ELISA was used to detect reactive oxygen species (ROS). RESULTS: Palmitate exposure greatly reduced miR-21 expression in cardiomyocytes. Apoptosis increased when miR-21 was inhibited with or without palmitate exposure. Consistently, reduced apoptosis was observed when miR-21 was overexpressed in cardiomyocytes. Caspase-3 activity was reduced after palmitate exposure. Bcl-2 protein expression was increased in H9c2 cells when transfected with the miR-21 mimic. MiR-21 overexpression alone did not induce ROS or DNA fragmentation; however, in conjunction with palmitate exposure, miR-21 mimic reduced ROS and DNA fragmentation. Moreover, palmitate administration overcame the antioxidant effect of 3 mM N-acetylcysteine to significantly inhibit apoptosis, DNA fragmentation, and caspase-3 activity. The exposure to palmitate greatly reduced p65 and p-p38 expression in the nucleus. A p38 inhibitor had no effect on the expression of Bcl-2 and cleaved caspase-3 in H9c2 cells alone; however, when combined with exposure to palmitate the p38 inhibitor induced Bcl-2 expression and inhibited caspase-3 activity. The p38 inhibitor by itself did not induce apoptosis, ROS production, or DNA fragmentation in H9c2 cells, but when palmitate was included with the p38 inhibitor, apoptosis, ROS production, and DNA fragmentation were reduced. CONCLUSION: miR-21 protects cardiomyocytes from apoptosis that is induced by palmitate through the caspase-3/NF-κB signal pathways.