RESUMEN
Suppressor of cytokine signaling (SOCS) family members are inhibitors of cytokine signaling pathways and key regulators of immunological homeostasis. They have been extensively studied in mammalian models, but systematic analyses of SOCS in fish are limited. In the current study, a total of eight SOCS genes from tongue sole (Cynoglossus semilaevis) were characterized. All eight CsSOCS exhibit conserved structures of SOCS and were phylogenetically grouped together with the respective SCOS members known in mammalian and teleost species. Under normal physiological conditions, the expressions of the eight CsSOCS genes were detected at varied levels in nine major tissues, with most CsSOCS highly expressed in kidney. Following challenge with intracellular and extracellular bacterial pathogens, the majority of CsSOCS genes exhibited distinctly different expression profiles in a time-, tissue-, and pathogen-dependent manner. In general, intracellular pathogen caused wider and higher levels of CsSOCS expressions than extracellular pathogen. These results suggest that different members of SOCSs in teleost may play different roles in the infection processes of different bacterial pathogens.
Asunto(s)
Infecciones por Enterobacteriaceae/veterinaria , Enfermedades de los Peces/genética , Proteínas de Peces/genética , Peces Planos , Expresión Génica , Proteínas Supresoras de la Señalización de Citocinas/genética , Vibriosis/veterinaria , Animales , Regulación hacia Abajo , Edwardsiella tarda/fisiología , Infecciones por Enterobacteriaceae/genética , Infecciones por Enterobacteriaceae/inmunología , Infecciones por Enterobacteriaceae/microbiología , Enfermedades de los Peces/inmunología , Enfermedades de los Peces/microbiología , Proteínas de Peces/química , Proteínas de Peces/metabolismo , Filogenia , ARN Mensajero/genética , ARN Mensajero/metabolismo , Análisis de Secuencia de Proteína , Proteínas Supresoras de la Señalización de Citocinas/química , Proteínas Supresoras de la Señalización de Citocinas/metabolismo , Regulación hacia Arriba , Vibrio/fisiología , Vibriosis/genética , Vibriosis/inmunología , Vibriosis/microbiologíaRESUMEN
Chemokines are a large, diverse group of small cytokines that can be classified into several families, including the CC chemokine family, which plays a pivotal role in host defense by inducing leukocyte chemotaxis under physiological and inflammatory conditions. Here we studied 9 CC chemokines from half-smooth tongue sole (Cynoglossus semilaevis). Phylogenetic analysis divided these chemokines into four groups. The tissue specific expression patterns of the 9 chemokines under normal physiological conditions varied much, with most chemokines highly expressed in immune organs, while some other chemokines showing high expression levels in non-immune organs. In addition, the 9 chemokines exhibited similar or distinctly different expression profiles in response to the challenge of virus and intracellular and extracellular bacterial pathogens. These results indicate that in tongue sole, CC chemokines may be involved in different immune responses as homeostatic or inflammatory chemokines.