Comparison of lactate measurements from earlobe and fingertip capillary blood using Biosen S-Line and lactate scout analyzers.

PURPOSE: This study aimed to compare variations between the earlobe and fingertip sampling sites in exercises dominated by upper body muscle exertion. It also sought to investigate capillary blood lactate differences between Lactate Scout 4 (LS4) and a bench-top analyzer (Biosen S-Line analyzer, BSL) during Double Poling. METHODS: Blood samples were collected from the earlobe and fingertip immediately before exercise, at the end of each of five stages, and at 1-, 3-, 5-, and 7-min post-exercise. Forty healthy university students participated as volunteers. During the study, they performed double poling on a ski ergometer with progressively increasing load. Lactate levels were measured using both the BSL and LS4 analyzers. RESULTS: Fingertip Bla values were significantly higher than earlobe values, with a mean bias of -0.66 mmol/L, reaching -0.86 mmol/L in the 4-8 mmol/L range. At the earlobe, the highest CCC between BSL and LS4-a was 0.84 (> 8 mmol/L), and for BSL and LS4-b, it was 0.85 (> 8 mmol/L). At the fingertip, the highest CCC between BSL and LS4-c was 0.68 (> 8 mmol/L), and for BSL and LS4-d, it was 0.52 (> 8 mmol/L). Comparing LS4-a and LS4-b at the earlobe, the highest CCC was 0.83 (0-4 mmol/L). At the fingertip, comparing LS4-c and LS4-d, the highest CCC was 0.68 (> 8 mmol/L). CONCLUSIONS: Blood lactate concentrations are higher at the fingertip than the earlobe during SkiErg double poling. The LS4 is less reliable, especially at the fingertip, so using the earlobe with the BSL analyzer is recommended for accurate measurements.

Associations between objective measures of performance-related characteristics and perceived stress in young cross-country skiers during pre-season training.

Monitoring performance-related characteristics of athletes can reveal changes that facilitate training adaptations. Here, we examine the relationships between submaximal running, maximal jump performance (CMJ), concentrations of blood lactate, sleep duration (SD) and latency (SL), and perceived stress (PSS) in junior cross-country skiers during pre-season training. These parameters were monitored in 15 male and 14 females (17 ± 1 years) for the 12-weeks prior to the competition season, and the data was analysed using linear and mixed-effect models. An increase in SD exerted a decrease in both PSS (B = -2.79, p ≤ 0.01) and blood lactate concentrations during submaximal running (B = -0.623, p ≤ 0.05). In addition, there was a negative relationship between SL and CMJ (B = -0.09, p = 0.08). Compared to males, females exhibited higher PSS scores and little or no change in performance-related tests. A significant interaction between time and sex was present in CMJ with males displaying an effect of time on CMJ performance. For all athletes, lower PSS appeared to be associated with longer overnight sleep. Since the females experienced higher levels of stress, monitoring of their PSS might be beneficial. These findings have implications for the preparation of young athletes' competition season.

Comparative Analysis of the Diagonal Stride Technique during Roller Skiing and On-Snow Skiing in Youth Cross-Country Skiers.

Roller skiing is one primary form of training method as it is an off-snow equivalent to cross-country (XC) skiing during the dry land preseason training, but the results could only be applied to on-snow skiing with appropriate caution. The aim of this present study was to investigate the similarities and differences in roller skiing and on-snow skiing with the diagonal stride (DS) technique. Six youth (age: 14.3 ± 2.9 years) skiers participated in this study. Two high-definition video camcorders and FastMove 3D Motion 2.23.3.3101 were used to obtain the three-dimensional kinematic data. The cycle characteristics and joint angle ROM of the DS technique while skiing on different surfaces were similar. Almost all joint angle-time curves that were obtained from roller skiing showed a moderate-to-high degree of similarity to the angle-time curves obtained from on-snow skiing, except the hip adduction-abduction angle. The differences between roller skiing and on-snow skiing were mainly found in the body and calf anteversion angles, and the joint angles at critical instants. DS roller skiing can simulate DS on-snow skiing to a large extent in youth athletes. The hip movement, knee flexion, and calf anteversion at ski/roller ski touchdown and take-off, pole inclination at pole touchdown, body anteversion angle, and trunk anteversion angle at pole touchdown were the points that required caution when transferring preseason practice roller skiing to on-snow skiing.

The magnitude and time-course of physiological responses to 9 weeks of incremental ramp testing.

PURPOSE: The aims of this study were to assess (1) the day-to-day variability in, and (2) the magnitude and time-course of adaptation of physiological parameters (i.e., maximal oxygen uptake [VO2 max], heart rate [HR], blood lactate concentration, respiratory exchange ratio [RER], ratings of perceived exertion [RPE], and time-to-exhaustion [TTE]) in response to an intervention involving three incremental ramp tests per week for 9 weeks. METHODS: Twelve participants (25 ± 4 yrs, VO2 max, 47.8 ± 5.2 mL∙min-1 ∙kg-1 (means ± SD)) completed the entire experimental procedure. The tests comprised a 5-min constant workload to obtain submaximal parameters followed by an incremental protocol until exhaustion. RESULTS: The mean day-to-day variability for the maximal value of VO2 was 2.8%, 1.1% for HR, 18.1% for blood lactate concentration, 2.1% for RER, 1.1% for RPE, and 5.0% for TTE. The values for the corresponding submaximal variables were 3.8% for VO2 , 2.1% for HR, 15.6% for blood lactate concentration, 2.6% for RER and 6.0% for RPE. VO2 max (+4.7% ± 3.5%), TTE (+17.9% ± 8.6%), and submaximal HR (-3.2 ± 3.5%) improved significantly. Except for RPE (p < 0.01), there were no alterations in the coefficient of variation for any parameter. On the group level, the first changes greater than the day-to-day variability in VO2 max, TTE, and submaximal HR were observed after 21, 12, and 9 training sessions, respectively. CONCLUSION: Based on our findings, we recommend that training studies include assessment of the reliability of the measurements, for example, the CVs in the specific laboratory to be able to judge if the changes detected are actually physiological.

The Validity of a Three-Dimensional Motion Capture System and the Garmin Running Dynamics Pod in Connection with an Assessment of Ground Contact Time While Running in Place.

A three-dimensional motion capture system (MoCap) and the Garmin Running Dynamics Pod can be utilised to monitor a variety of dynamic parameters during running. The present investigation was designed to examine the validity of these two systems for determining ground contact times while running in place by comparing the values obtained with those provided by the bilateral force plate (gold standard). Eleven subjects completed three 20-s runs in place at self-selected rates, starting slowly, continuing at an intermediate pace, and finishing rapidly. The ground contact times obtained with both systems differed significantly from the gold standard at all three rates, as well as for all the rates combined (p < 0.001 in all cases), with the smallest mean bias at the fastest step rate for both (11.5 ± 14.4 ms for MoCap and -81.5 ± 18.4 ms for Garmin). This algorithm was developed for the determination of ground contact times during normal running and was adapted here for the assessment of running in place by the MoCap, which could be one explanation for its lack of validity. In conclusion, the wearables developed for monitoring normal running cannot be assumed to be suitable for determining ground contact times while running in place.

A Comparison of a Novel Stretchable Smart Patch for Measuring Runner's Step Rates with Existing Measuring Technologies.

A novel wearable smart patch can monitor various aspects of physical activity, including the dynamics of running, but like any new device developed for such applications, it must first be tested for validity. Here, we compare the step rate while running in place as measured by this smart patch to the corresponding values obtained utilizing ''gold standard'' MEMS accelerometers in combination with bilateral force plates equipped with HBM load cells, as well as the values provided by a three-dimensional motion capture system and the Garmin Dynamics Running Pod. The 15 healthy, physically active volunteers (age = 23 ± 3 years; body mass = 74 ± 17 kg, height = 176 ± 10 cm) completed three consecutive 20-s bouts of running in place, starting at low, followed by medium, and finally at high intensity, all self-chosen. Our major findings are that the rates of running in place provided by all four systems were valid, with the notable exception of the fast step rate as measured by the Garmin Running Pod. The lowest mean bias and LoA for these measurements at all rates were associated consistently with the smart patch.

A Systematic Review of the Effects of Strength and Power Training on Performance in Cross-Country Skiers.

To identify and evaluate current scientific literature concerning the effect of strength, power and speed training on relevant physiological and biomechanical characteristics and performance of competitive cross-country skiers (XCS), the databases Scopus and PubMed were searched systematically for original articles in peer-reviewed journals. Of the 599 studies retrieved, 12 met the inclusion criteria (i.e., assessment of outcome measures with relevance for XCS performance; involvement of traditional resistance training; application of external resistance to the body; intervention longer than 4 weeks; randomized controlled trial). The methodological rigor of each study was assessed using the PEDro scale, which were mostly poor-to-fair, with good methodological quality in only two articles. All of the strength/power/speed interventions improved 1RM (0.8-6.8 ES), but findings with respect to jump performance, ability to generate force rapidly and body composition were mixed. Interventions demonstrated moderate-to-high ES on XCS specific performance compared with control (mean ES = 0.56), but the pattern observed was not consistent. None of the interventions changed anaerobic capacity, while in most studies VO2max was either unchanged or increased. Work economy or efficiency was enhanced by most of the interventions. In conclusion, present research indicates that strength training improves general strength, with moderate effects on XCS performance, and inconclusive effects on work economy and VO2max/VO2peak. Strength training with high loads, explosive strength training, or sprint interval training seem to be promising tools for modern XCS training. Future investigations should include long-term (e.g., >6 months) strength training to allow sufficient time for increased strength and speed to influence actual XCS performance. Moreover, they should include both sexes, as well as upper- and lower-body muscles (trained separately and together) and employ free weights and core training. Methodological differences and limitations highlighted here may explain discrepancies in findings and should be taken into consideration in future research in this area.

Glycogen supercompensation is due to increased number, not size, of glycogen particles in human skeletal muscle.

NEW FINDINGS: What is the central question of this study? Glycogen supercompensation after glycogen-depleting exercise can be achieved by consuming a carbohydrate-enriched diet, but the associated effects on the size, number and localization of intramuscular glycogen particles are unknown. What is the main finding and its importance? Using transmission electron microscopy to inspect individual glycogen particles visually, we show that glycogen supercompensation is achieved by increasing the number of particles while keeping them at submaximal sizes. This might be a strategy to ensure that glycogen particles can be used fast, because particles that are too large might impair utilization rate. ABSTRACT: Glycogen supercompensation after glycogen-depleting exercise can be achieved by consuming a carbohydrate-enriched diet, but the associated effects on the size, number and localization of intramuscular glycogen particles are unknown. We investigated how a glycogen-loading protocol affects fibre type-specific glycogen volume density, particle diameter and numerical density in three subcellular pools: between (intermyofibrillar) or within (intramyofibrillar) the myofibrils or beneath the sarcolemma (subsarcolemmal). Resting muscle biopsies from 11 physically active men were analysed using transmission electron microscopy after mixed (MIX), LOW or HIGH carbohydrate consumption separated by glycogen-lowering cycling at 75% of maximal oxygen consumption until exhaustion. After HIGH, the total volumetric glycogen content was 40% [95% confidence interval 16, 68] higher than after MIX in type I fibres (P < 0.001), with little to no difference in type II fibres (9% [95% confidence interval -9, 27]). Median particle diameter was 22.5 (interquartile range 20.8-24.7) nm across glycogen pools and fibre types, and the numerical density was 61% [25, 107] and 40% [9, 80] higher in the subsarcolemmal (P < 0.001) and intermyofibrillar (P < 0.01) pools of type I fibres, respectively, with little to no difference in the intramyofibrillar pool (3% [-20, 32]). In LOW, total glycogen was in the range of 21-23% lower, relative to MIX, in both fibre types, reflected in a 21-46% lower numerical density across pools. In comparison to MIX, particle diameter was unaffected by other diets ([-1.4, 1.3] nm). In conclusion, glycogen supercompensation after prolonged cycling is exclusive to type I fibres, predominantly in the subsarcolemmal pool, and involves an increase in the numerical density rather than the size of existing glycogen particles.

Angiotensin-Converting Enzyme 2 (SARS-CoV-2 receptor) expression in human skeletal muscle.

The study aimed to determine the levels of skeletal muscle angiotensin-converting enzyme 2 (ACE2, the SARS-CoV-2 receptor) protein expression in men and women and assess whether ACE2 expression in skeletal muscle is associated with cardiorespiratory fitness and adiposity. The level of ACE2 in vastus lateralis muscle biopsies collected in previous studies from 170 men (age: 19-65 years, weight: 56-137 kg, BMI: 23-44) and 69 women (age: 18-55 years, weight: 41-126 kg, BMI: 22-39) was analyzed in duplicate by western blot. VO2 max was determined by ergospirometry and body composition by DXA. ACE2 protein expression was 1.8-fold higher in women than men (p = 0.001, n = 239). This sex difference disappeared after accounting for the percentage of body fat (fat %), VO2 max per kg of legs lean mass (VO2 max-LLM) and age (p = 0.47). Multiple regression analysis showed that the fat % (ß = 0.47) is the main predictor of the variability in ACE2 protein expression in skeletal muscle, explaining 5.2% of the variance. VO2 max-LLM had also predictive value (ß = 0.09). There was a significant fat % by VO2 max-LLM interaction, such that for subjects with low fat %, VO2 max-LLM was positively associated with ACE2 expression while as fat % increased the slope of the positive association between VO2 max-LLM and ACE2 was reduced. In conclusion, women express higher amounts of ACE2 in their skeletal muscles than men. This sexual dimorphism is mainly explained by sex differences in fat % and cardiorespiratory fitness. The percentage of body fat is the main predictor of the variability in ACE2 protein expression in human skeletal muscle.

Reliability and Validity of the CORE Sensor to Assess Core Body Temperature during Cycling Exercise.

Monitoring core body temperature (Tc) during training and competitions, especially in a hot environment, can help enhance an athlete's performance, as well as lower the risk for heat stroke. Accordingly, a noninvasive sensor that allows reliable monitoring of Tc would be highly beneficial in this context. One such novel non-invasive sensor was recently introduced onto the market (CORE, greenTEG, Rümlang, Switzerland), but, to our knowledge, a validation study of this device has not yet been reported. Therefore, the purpose of this study was to evaluate the validity and reliability of the CORE sensor. In Study I, 12 males were subjected to a low-to-moderate heat load by performing, on two separate occasions several days apart, two identical 60-min bouts of steady-state cycling in the laboratory at 19 °C and 30% relative humidity. In Study II, 13 males were subjected to moderate-to-high heat load by performing 90 min of cycling in the laboratory at 31 °C and 39% relative humidity. In both cases the core body temperatures indicated by the CORE sensor were compared to the corresponding values obtained using a rectal sensor (Trec). The first major finding was that the reliability of the CORE sensor is acceptable, since the mean bias between the two identical trials of exercise (0.02 °C) was not statistically significant. However, under both levels of heat load, the body temperature indicated by the CORE sensor did not agree well with Trec, with approximately 50% of all paired measurements differing by more than the predefined threshold for validity of ≤0.3 °C. In conclusion, the results obtained do not support the manufacturer's claim that the CORE sensor provides a valid measure of core body temperature.

Relationships between Heart Rate Variability, Sleep Duration, Cortisol and Physical Training in Young Athletes.

The aims of the current study were to examine the relationships between heart rate variability (HRV), salivary cortisol, sleep duration and training in young athletes. Eight athletes (16 ± 1 years) were monitored for 7 weeks during training and competition seasons. Subjects were training for endurance-based winter sports (cross-country skiing and biathlon). Training was divided into two zones (K1, easy training and K2, hard training). Heart rate and blood lactate during submaximal running tests (SRT), as well as cortisol, sleep duration and nocturnal HRV (RMSSD), were determined every other week. HRV and cortisol levels were correlated throughout the 7-week period (r = -0.552, P = 0.01), with the strongest correlation during week 7 (r = -0.879, P = 0.01). The relative changes in K1 and HRV showed a positive correlation from weeks 1-3 (r = 0.863, P = 0.006) and a negative correlation during weeks 3-5 (r = -0.760, P = 0.029). The relative change in sleep during weeks 1-3 were negatively correlated with cortisol (r = -0.762, P = 0.028) and K2 (r = -0.762, P = 0.028). In conclusion, HRV appears to reflect the recovery of young athletes during high loads of physical and/or physiological stress. Cortisol levels also reflected this recovery, but significant change required a longer period than HRV, suggesting that cortisol may be less sensitive to stress than HRV. Moreover, our results indicated that during the competition season, recovery for young endurance athletes increased in duration and additional sleep may be beneficial.

Heterogeneity in subcellular muscle glycogen utilisation during exercise impacts endurance capacity in men.

KEY POINTS: When muscle biopsies first began to be used routinely in research on exercise physiology five decades ago, it soon become clear that the muscle content of glycogen is an important determinant of exercise performance. Glycogen particles are stored in distinct pools within the muscles, but the role of each pool during exercise and how this is affected by diet is unknown. Here, the effects of diet and exercise on these pools, as well as their relation to endurance during prolonged cycling were examined. We demonstrate here that an improved endurance capacity with high carbohydrate loading is associated with a temporal shift in the utilisation of the distinct stores of glycogen pools and is closely linked to the content of the glycogen pool closest to actin and myosin (intramyofibrillar glycogen). These findings highlight the functional importance of distinguishing between different subcellular microcompartments of glycogen in individual muscle fibres. ABSTRACT: In muscle cells, glycogen is stored in three distinct subcellular pools: between or within myofibrils (inter- and intramyofibrillar glycogen, respectively) or beneath the sarcolemma (subsarcolemmal glycogen) and these pools may well have different functions. Here, we investigated the effect of diet and exercise on the content of these distinct pools and their relation to endurance capacity in type 1 and 2 muscle fibres. Following consumption of three different diets (normal, mixed diet = MIX, high in carbohydrate = HIGH, or low in carbohydrate = LOW) for 72 h, 11 men cycled at 75% of V̇O2 max until exhaustion. The volumetric content of the glycogen pools in muscle biopsies obtained before, during, and after exercise were quantified by transmission electron micrographs. The mean (SD) time to exhaustion was 150 (30), 112 (22), and 69 (18) minutes in the HIGH, MIX and LOW trials, respectively (P < 0.001). As shown by multiple regression analyses, the intramyofibrillar glycogen content in type 1 fibres, particularly after 60 min of exercise, correlated most strongly with time to exhaustion. In the HIGH trial, intramyofibrillar glycogen was spared during the initial 60 min of exercise, which was associated with levels and utilisation of subsarcolemmal glycogen above normal. In all trials, utilisation of subsarcolemmal and intramyofibrillar glycogen was more pronounced than that of intermyofibrillar glycogen in relative terms. In conclusion, the muscle pool of intramyofibrillar glycogen appears to be the most important for endurance capacity in humans. In addition, a local abundance of subsarcolemmal glycogen reduces the utilisation of intramyofibrillar glycogen during exercise.

Elevated plasma lactate levels via exogenous lactate infusion do not alter resistance exercise-induced signaling or protein synthesis in human skeletal muscle.

Lactate has been implicated as a potential signaling molecule. In myotubes, lactate incubation increases mechanistic target of rapamycin complex 1 (mTORC1)- and ERK-signaling and induces hypertrophy, indicating that lactate could be a mediator of muscle adaptations to resistance exercise. However, the potential signaling properties of lactate, at rest or with exercise, have not been explored in human tissue. In a crossover design study, 8 men and 8 women performed one-legged resistance exercise while receiving venous infusion of saline or sodium lactate. Blood was sampled repeatedly, and muscle biopsies were collected at rest and at 0, 90, and 180 min and 24 h after exercise. The primary outcomes examined were intracellular signaling, fractional protein synthesis rate (FSR), and blood/muscle levels of lactate and pH. Postexercise blood lactate concentrations were 130% higher in the Lactate trial (3.0 vs. 7.0 mmol/L, P < 0.001), whereas muscle levels were only marginally higher (27 vs. 32 mmol/kg dry wt, P = 0.003) compared with the Saline trial. Postexercise blood pH was higher in the Lactate trial (7.34 vs. 7.44, P < 0.001), with no differences in intramuscular pH. Exercise increased the phosphorylation of mTORS2448 (∼40%), S6K1T389 (∼3-fold), and p44T202/T204 (∼80%) during recovery, without any differences between trials. FSR over the 24-h recovery period did not differ between the Saline (0.067%/h) and Lactate (0.062%/h) trials. This study does not support the hypothesis that blood lactate levels can modulate anabolic signaling in contracted human muscle. Further in vivo research investigating the impact of exercised versus rested muscle and the role of intramuscular lactate is needed to elucidate its potential signaling properties.

Predefined vs data-guided training prescription based on autonomic nervous system variation: A systematic review.

Monitoring variations in the functioning of the autonomic nervous system may help personalize training of runners and provide more pronounced physiological adaptations and performance improvements. We systematically reviewed the scientific literature comparing physiological adaptations and/or improvements in performance following training based on responses of the autonomic nervous system (ie, changes in heart rate variability) and predefined training. PubMed, SPORTDiscus, and Web of Science were searched systematically in July 2019. Keywords related to endurance, running, autonomic nervous system, and training. Studies were included if they (a) involved interventions consisting predominantly of running training; (b) lasted at least 3 weeks; (c) reported pre- and post-intervention assessment of running performance and/or physiological parameters; (d) included an experimental group performing training adjusted continuously on the basis of alterations in HRV and a control group; and (e) involved healthy runners. Five studies involving six interventions and 166 participants fulfilled our inclusion criteria. Four HRV-based interventions reduced the amount of moderate- and/or high-intensity training significantly. In five interventions, improvements in performance parameters (3000 m, 5000 m, Loadmax, Tlim) were more pronounced following HRV-based training. Peak oxygen uptake ( V ˙ O 2 peak ) and submaximal running parameters (eg, LT1, LT2) improved following both HRV-based and predefined training, with no clear difference in the extent of improvement in V ˙ O 2 peak . Submaximal running parameters tended to improve more following HRV-based training. Research findings to date have been limited and inconsistent. Both HRV-based and predefined training improve running performance and certain submaximal physiological adaptations, with effects of the former training tending to be greater.

Sarcolipin expression in human skeletal muscle: Influence of energy balance and exercise.

Sarcolipin (SLN) is a SERCA uncoupling protein associated with exercise performance and lower adiposity in mice. To determine SLN protein expression in human skeletal muscle and its relationship with adiposity, resting energy expenditure (REE), and performance, SLN was assessed by Western blot in 199 biopsies from two previous studies. In one study, 15 overweight volunteers underwent a pretest followed by 4 days of caloric restriction and exercise (45-minute one-arm cranking + 8-hour walking), and 3 days on a control diet. Muscle biopsies were obtained from the trained and non-exercised deltoid, and vastus lateralis (VL). In another study, 16 men performed seven sessions of 4-6 × 30-sec all-out sprints on the cycle ergometer with both limbs, and their VL and triceps brachii biopsied pre- and post-training. SLN expression was twofold and 44% higher in the VL than in the deltoids and triceps brachii, respectively. SLN was associated with neither adiposity nor REE, and was not altered by a severe energy deficit (5500 kcal/day). SLN and cortisol changes after the energy deficit were correlated (r = .38, P = .039). SLN was not altered by low-intensity exercise in the overweight subjects, whereas it was reduced after sprint training in the other group. The changes in SLN with sprint training were inversely associated with the changes in gross efficiency (r = -.59, P = .016). No association was observed between aerobic or anaerobic performance and SLN expression. In conclusion, sarcolipin appears to play no role in regulating the fat mass of men. Sprint training reduces sarcolipin expression, which may improve muscle efficiency.

Intra-individual physiological response of recreational runners to different training mesocycles: a randomized cross-over study.

PURPOSE: Pronounced differences in individual physiological adaptation may occur following various training mesocycles in runners. Here we aimed to assess the individual changes in performance and physiological adaptation of recreational runners performing mesocycles with different intensity, duration and frequency. METHODS: Employing a randomized cross-over design, the intra-individual physiological responses [i.e., peak ([Formula: see text]) and submaximal ([Formula: see text]) oxygen uptake, velocity at lactate thresholds (V2, V4)] and performance (time-to-exhaustion (TTE)) of 13 recreational runners who performed three 3-week sessions of high-intensity interval training (HIIT), high-volume low-intensity training (HVLIT) or more but shorter sessions of HVLIT (high-frequency training; HFT) were assessed. RESULTS: [Formula: see text], V2, V4 and TTE were not altered by HIIT, HVLIT or HFT (p > 0.05). [Formula: see text] improved to the same extent following HVLIT (p = 0.045) and HFT (p = 0.02). The number of moderately negative responders was higher following HIIT (15.4%); and HFT (15.4%) than HVLIT (7.6%). The number of very positive responders was higher following HVLIT (38.5%) than HFT (23%) or HIIT (7.7%). 46% of the runners responded positively to two mesocycles, while 23% did not respond to any. CONCLUSION: On a group level, none of the interventions altered [Formula: see text], V2, V4 or TTE, while HVLIT and HFT improved [Formula: see text]. The mean adaptation index indicated similar numbers of positive, negative and non-responders to HIIT, HVLIT and HFT, but more very positive responders to HVLIT than HFT or HIIT. 46% responded positively to two mesocycles, while 23% did not respond to any. These findings indicate that the magnitude of responses to HIIT, HVLIT and HFT is highly individual and no pattern was apparent.

Biomechanical determinants of cross-country skiing performance: A systematic review.

Cross-country skiing is a complex endurance sport requiring technical skills, in addition to considerable physiological and tactical abilities. This review aims to identify biomechanical factors that influence the performance of cross-country skiers. Four electronic databases were searched systematically for original articles in peer-reviewed journals addressing the relationship between biomechanical factors (including kinematics, kinetics, and muscle activation) and performance while skiing on snow or roller skiing. Of the 46 articles included, 22 focused exclusively on the classical technique, 18 on the skating technique, and six on both. The indicators of performance were: results from actual or simulated races (9 articles); speed on specific tracts (6 articles); maximal or peak speed (11 articles); skiing economy or efficiency (11 articles); and grouping on the basis of performance or level of skill (12 articles). The main findings were that i) cycle length, most often considered as a major determinant of skiing speed, is also related to skiing economy and level of performance; ii) higher cycle rate related with maximal speed capacity, while self-selected cycle rate improves skiing economy at sub-maximal speeds; iii) cross-country skiing performance appears to be improved by joint, whole-body, ski, and pole kinematics that promote forward propulsion while minimizing unnecessary movement.

Protein synthesis signaling in skeletal muscle is refractory to whey protein ingestion during a severe energy deficit evoked by prolonged exercise and caloric restriction.

BACKGROUND: Exercise and protein ingestion preserve muscle mass during moderate energy deficits. OBJECTIVE: To determine the molecular mechanisms by which exercise and protein ingestion may spare muscle mass during severe energy deficit (5500 kcal/day). DESIGN: Fifteen overweight, but otherwise healthy men, underwent a pre-test (PRE), caloric restriction (3.2 kcals/kg body weight/day) + exercise (45 min one-arm cranking + 8 h walking) for 4 days (CRE), followed by a control diet (CD) for 3 days, with a caloric content similar to pre-intervention while exercise was reduced to less than 10,000 steps per day. During CRE, participants ingested either whey protein (PRO, n = 8) or sucrose (SU, n = 7) (0.8 g/kg body weight/day). Muscle biopsies were obtained from the trained and untrained deltoid, and vastus lateralis. RESULTS: Following CRE and CD, serum concentrations of leptin, insulin, and testosterone were reduced, whereas cortisol and the catabolic index (cortisol/total testosterone) increased. The Akt/mTor/p70S6K pathway and total eIF2α were unchanged, while total 4E-BP1 and Thr37/464E-BP1 were higher. After CRE, plasma BCAA and EAA were elevated, with a greater response in PRO group, and total GSK3ß, pSer9GSK3ß, pSer51eIF2α, and pSer51eIF2α/total eIF2α were reduced, with a greater response of pSer9GSK3ß in the PRO group. The changes in signaling were associated with the changes in leptin, insulin, amino acids, cortisol, cortisol/total testosterone, and lean mass. CONCLUSIONS: During severe energy deficit, pSer9GSK3ß levels are reduced and human skeletal muscle becomes refractory to the anabolic effects of whey protein ingestion, regardless of contractile activity. These effects are associated with the changes in lean mass and serum insulin, testosterone, and cortisol concentrations.

The impact of start strategy on start performance in alpine skiing exists on flat, but not on steep inclines.

Here, we explored the relationship between incline and start strategy during alpine skiing. Eight FIS skiers performed starts on a flat (3°) and steep (21°) incline employing five different strategies. Their times, trajectories and velocities were monitored with a GNSS system and video. A significant interaction was observed between slope incline and start strategy with respect to the skier's exit velocity (p < 0.001, ƞ2p = 0.716), but not for the start section time (p = 0.732, ƞ2p = 0.037). On the almost flat incline, both section time (p = 0.022, ƞ2p = 0.438) and exit velocity (p < 0.001, ƞ2p = 0.786) were influenced significantly by start strategy, with four V2 skate-pushes being optimal. On the steep incline, neither section time nor exit velocity was affected significantly by start strategy, the fastest section time and exit velocity being attained with four and two V2 skate-pushes, respectively. In conclusion, these findings demonstrate that the start strategy exerts considerable impact on start performance on almost flat inclines, with strategies involving three or more V2 skate-pushes being optimal. In contrast, start performance on the steep incline was not influenced by strategy.

Integrated Framework of Load Monitoring by a Combination of Smartphone Applications, Wearables and Point-of-Care Testing Provides Feedback that Allows Individual Responsive Adjustments to Activities of Daily Living.

Athletes schedule their training and recovery in periods, often utilizing a pre-defined strategy. To avoid underperformance and/or compromised health, the external load during training should take into account the individual's physiological and perceptual responses. No single variable provides an adequate basis for planning, but continuous monitoring of a combination of several indicators of internal and external load during training, recovery and off-training as well may allow individual responsive adjustments of a training program in an effective manner. From a practical perspective, including that of coaches, monitoring of potential changes in health and performance should ideally be valid, reliable and sensitive, as well as time-efficient, easily applicable, non-fatiguing and as non-invasive as possible. Accordingly, smartphone applications, wearable sensors and point-of-care testing appear to offer a suitable monitoring framework allowing responsive adjustments to exercise prescription. Here, we outline 24-h monitoring of selected parameters by these technologies that (i) allows responsive adjustments of exercise programs, (ii) enhances performance and/or (iii) reduces the risk for overuse, injury and/or illness.