RESUMEN
We collected 312 samples from hospitalized patients in two hospitals in Timisoara between September-December 2003. We isolated 83 strains with nosocomial potential. Identification of the germs was performed using the automatic API system, and the susceptibility tests were performed using disc-diffusion and the agar dilution test. By analyzing the extended antibiograms we categorized the germs considering their phenotypes of resistance and we remarked a high percentage of E. coli, Klebsiella pneumoniae pneumoniae and S. aureus with multiple resistance to antibiotics.
Asunto(s)
Infecciones Bacterianas/microbiología , Infección Hospitalaria/microbiología , Escherichia coli/aislamiento & purificación , Klebsiella pneumoniae/aislamiento & purificación , Staphylococcus aureus/aislamiento & purificación , Antibacterianos/uso terapéutico , Infecciones Bacterianas/tratamiento farmacológico , Moco del Cuello Uterino/microbiología , Infección Hospitalaria/tratamiento farmacológico , Resistencia a Múltiples Medicamentos , Escherichia coli/efectos de los fármacos , Femenino , Humanos , Klebsiella pneumoniae/efectos de los fármacos , Masculino , Pruebas de Sensibilidad Microbiana , Servicio de Ginecología y Obstetricia en Hospital , Estudios Retrospectivos , Rumanía , Staphylococcus aureus/efectos de los fármacos , Orina/microbiología , Servicio de Urología en HospitalRESUMEN
The study was designed to determine the chemical composition and antimicrobial properties of the essential oil of Thymus vulgaris cultivated in Romania. The essential oil was isolated in a yield of 1.25% by steam distillation from the aerial part of the plant and subsequently analyzed by GC-MS. The major components were p-cymene (8.41%), γ-terpinene (30.90%) and thymol (47.59%). Its antimicrobial activity was evaluated on 7 common food-related bacteria and fungus by using the disk diffusion method. The results demonstrate that the Thymus vulgaris essential oil tested possesses strong antimicrobial properties, and may in the future represent a new source of natural antiseptics with applications in the pharmaceutical and food industry.