RESUMEN
A cytogenetic analysis of 14 primary testicular nonseminomatous germ cell tumors has been carried out after short term tissue culture. The modal chromosome numbers ranged from 53 to 113, in agreement with flow cytometric determination of the DNA content of the tumors. At least one copy of an i(12p) was present in 12 tumors. Two tumors, however, lacked that marker. Some chromosomes are apparently overrepresented, whereas others are underrepresented, although some differences between seminomas and nonseminomas were noticed.
Asunto(s)
Aberraciones Cromosómicas/patología , Neoplasias de Células Germinales y Embrionarias/genética , Neoplasias Testiculares/genética , Análisis de Varianza , Aneuploidia , Trastornos de los Cromosomas , Humanos , Cariotipificación , Masculino , Neoplasias de Células Germinales y Embrionarias/patología , PloidiasRESUMEN
A cytogenetic analysis of 13 mature residual teratomas following chemotherapy revealed modal chromosome numbers ranging from 52 to 85, in agreement with the flow cytometric determination of the DNA content of the tumors. At least one copy of an i(12p) was present in 12 tumors. One tumor, however, lacked that marker. The comparison between the chromosomal abnormalities found in mature residual teratomas following chemotherapy and those from primary testicular nonseminomas suggests that residual teratomas result from selection of clones from the primary tumor with a less abnormal karyotype.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Aberraciones Cromosómicas , Trastornos de los Cromosomas , Teratoma/genética , Neoplasias Testiculares/genética , Adulto , Citometría de Flujo , Humanos , Cariotipificación , Masculino , Persona de Mediana Edad , Teratoma/tratamiento farmacológico , Neoplasias Testiculares/tratamiento farmacológicoRESUMEN
Lymphocyte cultures from the Chinese hamster were made with lymphocytes isolated with Ficoll-Isopaque or with Haemaccel. In some experiments the red blood cells (r.b.c.) that remained after lymphocyte isolation were lysed. Optimum phytohaemagglutinin (PHA) concentration for lymphocyte proliferation depended upon the manner in which the lymphocytes were obtained. Lower PHA concentrations were needed for optimum stimulation of lymphocytes isolated with Ficoll-Isopaque than for lymphocytes isolated with Haemaccel. Lysis of the r.b.c. remaining after isolation of lymphocytes with Haemaccel resulted in a decrease of optimum PHA concentration. The optimum PHA concentration depended strongly on r.b.c. contamination, the higher the r.b.c. contamination the higher the optimum PHA concentration. However, only part of the differences found in optimum PHA concentrations can be attributed to r.b.c. contamination.
Asunto(s)
Eritrocitos , Activación de Linfocitos , Linfocitos/inmunología , Fitohemaglutininas , Animales , Células Cultivadas , Cricetinae , Medios de Cultivo , Técnicas de Cultivo/métodos , Relación Dosis-Respuesta a DrogaRESUMEN
Chinese hamster lymphocyte cultures were supplemented with foetal calf serum (FCS) or hose serum (HS). Addition of 2-mercaptoethanol (2-ME) to cultures supplemented with these sera resulted in a marked increase lymphocyte proliferation. The PHA concentrations necessary for optimal lymphocyte stimulation were much lower in cultures supplemented with HS than with FCS. Phytohaemagglutinin (PHA) concentrations optimal for stimulation of a given number of lymphocytes in cultures supplemented with FCS often gave inhibition of similar cultures supplemented with HS.
Asunto(s)
Sangre , Sangre Fetal , Activación de Linfocitos , Fitohemaglutininas/farmacología , Animales , Bovinos , Células Cultivadas , Cricetinae , Cricetulus , Medios de Cultivo , Relación Dosis-Respuesta Inmunológica , Femenino , Caballos , Técnicas Inmunológicas , Mercaptoetanol/farmacología , EmbarazoRESUMEN
Cytogenetic analysis was performed of three seminomas, two primary nonseminomas, and two mature residual teratomas following chemotherapy, all lacking i(12p). Testicular germ cell tumors without an i(12p) may represent a subgroup of germ cell tumors, also in their clinical course, compared with those having i(12p).
Asunto(s)
Aberraciones Cromosómicas , Cromosomas Humanos Par 12 , Neoplasias de Células Germinales y Embrionarias/genética , Neoplasias Testiculares/genética , Adulto , Bandeo Cromosómico , Marcadores Genéticos , Humanos , Cariotipificación , Masculino , Persona de Mediana Edad , Neoplasias de Células Germinales y Embrionarias/clasificación , Teratoma/genética , Neoplasias Testiculares/clasificaciónRESUMEN
The cytogenetics of 12 cases of renal adenocarcinoma are presented. Clonal abnormalities were found in nine patients. Worth mentioning are abnormalities of the X chromosome (1 X) and the chromosomes #1(3 X), #3(3 X), #7(4 X), #8(4 X), and #14(2 X). From our data and data from the literature it appeared that the chromosomal regions 3p11-p21, 1q21, 14q22, and Xp11 are important for the oncogenesis of renal cell carcinoma as well as increased dosage of chromosome #7 and loss or abnormalities of chromosomes #8 and #14.
Asunto(s)
Carcinoma de Células Renales/genética , Aberraciones Cromosómicas , Neoplasias Renales/genética , Adolescente , Adulto , Anciano , Niño , Preescolar , Bandeo Cromosómico , Femenino , Marcadores Genéticos , Humanos , Cariotipificación , Masculino , Persona de Mediana EdadAsunto(s)
Linfocitos B/fisiología , Cadenas kappa de Inmunoglobulina/genética , Leucemia/genética , Animales , Anticuerpos Monoclonales , Antígenos de Superficie/análisis , Linfocitos B/patología , Diferenciación Celular , Citoplasma/inmunología , Cadenas kappa de Inmunoglobulina/análisis , Cadenas mu de Inmunoglobulina/análisis , Cariotipificación , Leucemia/inmunología , Leucemia/patología , Ratas , Receptores de Antígenos de Linfocitos B/análisis , Recombinación GenéticaRESUMEN
Cell hybridization was done between a monolayer of B14-150 Chinese hamster cells and a suspension of either mouse leukemia cells or normal human lymphocytes. Cell contact was obtained by centrifugation of the suspension cells onto the monolayer cells in a culture plate. Cell fusion was done by means of polyethylene glycol (PEG). The optimum time for PEG exposure as well as the yield of hybrid cells differed markedly with the different combinations.
Asunto(s)
Fusión Celular , Polietilenglicoles , Animales , Línea Celular , Técnicas de Cultivo/métodos , Humanos , Leucemia Experimental , Linfocitos , Factores de TiempoRESUMEN
Seventeen soft tissue sarcomas were karyotyped and the chromosomal findings were compared with those in the literature. Some cases were easily classified (immuno)histologically, whereas others defied classification. In four cases with unequivocal histology, a "characteristic" chromosomal abnormality was found. In one case a diagnosis that was doubtful on histologic grounds was supported by the chromosomal findings, whereas in three other cases, the chromosomal pattern led to reconsideration of the histologic diagnosis. The remaining cases either showed chromosomal abnormalities with hitherto undescribed breakpoints or breakpoints described in tumors with different histology. The results extend the knowledge of chromosomal abnormalities in soft tissue sarcomas. Most importantly, the present approach demonstrates that close collaboration between the surgical pathologist and the cytogeneticist may improve the classification of soft tissue sarcomas.