RESUMEN
BACKGROUND/AIMS: Junctional adhesion molecules (JAMs) are known as integral constituents of cellular tight junctions. However, the functions of JAMs in cancer tissues are controversial and the function of JAM-A in gastric cancer is unclear. Acordingly, we investigated the function of JAM-A in gastric epithelial and gastric cancer cell proliferation, invasion and apoptosis. METHODOLOGY: A normal rat gastric mucosa-derived cell line (RGM1), a rat gastric cancer-like cell line established from RGM1 (RGK1), and a human gastric cancer cell line (NCI-N87) were used in this study. To examine the expression of junctional proteins, immunoblotting and immunofluorescent staining were performed with specific antibodies (JAM-A, claudins, occludin and ZO-1). JAM-A was knocked down by small interfering RNA. RESULTS: RGM1 and RGK1 expressed JAM-A, occludin and ZO-1 but not claudins. RGK1 were significantly more invasive than RGM1. JAM-A knock-down significantly decreased the proliferation and the invasion of RGK1 but not of RGM1. JAM-A knock-down significantly decreased the proliferation of NCI-N87 cells and significantly decreased expression of the anti-apoptotic protein Bcl-xL but not the expression of AKT or Mcl-1. CONCLUSIONS: JAM-A promotes proliferation and inhibits apoptosis of gastric cancer, suggesting that it has a pivotal role in gastric cancer progression.
Asunto(s)
Apoptosis , Moléculas de Adhesión Celular/metabolismo , Proliferación Celular , Mucosa Gástrica/metabolismo , Receptores de Superficie Celular/metabolismo , Neoplasias Gástricas/metabolismo , Animales , Moléculas de Adhesión Celular/genética , Línea Celular Tumoral , Movimiento Celular , Claudinas/metabolismo , Mucosa Gástrica/patología , Humanos , Invasividad Neoplásica , Ocludina/metabolismo , Interferencia de ARN , Ratas , Receptores de Superficie Celular/genética , Transducción de Señal , Neoplasias Gástricas/genética , Neoplasias Gástricas/patología , Transfección , Proteína de la Zonula Occludens-1/metabolismo , Proteína bcl-X/metabolismoRESUMEN
BACKGROUND: With the increase in endoscopic procedures, endoscopists are spending more time creating reports. Although medical reports have largely become electronic, most of the current reporting systems require manual operation. This study aimed to evaluate the efficacy of a novel endoscopic reporting system that uses voice recognition (VR) technology. METHODS: We retrospectively reviewed consecutive patients who underwent esophagogastroduodenoscopy between September 2019 and March 2020 at a general hospital in Japan. The novel reporting system, used during endoscopic procedures, is equipped with VR and provides automatic responses by playing back recognized words. Differences in total time spent on the endoscopic procedure and report preparation between the manual entry (ME) and VR groups were evaluated using a propensity score matching method. RESULTS: We enrolled 356 patients: 226 and 130 patients in the ME and VR groups, respectively. Propensity score matching created 101 matched pairs. After matching, the median report preparation time (311 vs. 383 s, P = 0.009) and median total time (765 vs. 842 s, P = 0.053) in the VR group were shorter than those in the ME group. The VR system independently shortened the total and report preparation times by 156 s (95% confidence interval, - 274 to - 37 s; P = 0.009) and 118 s (95% confidence interval, - 220 to - 15 s; P = 0.023), respectively, on multiple linear regression analysis. CONCLUSIONS: The VR system could save the report preparation time and the total time. This novel system may improve the efficiency of endoscopy-related tasks.
Asunto(s)
Endoscopía Gastrointestinal , Reconocimiento de Voz , Humanos , Japón , Puntaje de Propensión , Estudios Retrospectivos , Resultado del TratamientoAsunto(s)
Neoplasias de los Conductos Biliares , Carcinoma , Colitis Ulcerosa , Enfermedad de Crohn , Infecciones por Virus de Epstein-Barr , Humanos , Colitis Ulcerosa/complicaciones , Colitis Ulcerosa/patología , Herpesvirus Humano 4 , Enfermedad de Crohn/complicaciones , Infecciones por Virus de Epstein-Barr/complicaciones , Neoplasias de los Conductos Biliares/complicaciones , Conductos Biliares/patología , Carcinoma/complicacionesRESUMEN
A 47-year-old woman was diagnosed as having advanced gastric cancer with malignant ascites. Despite chemotherapy, recurrent peritoneal dissemination was seen 1.5 years after operation. A computed tomography scan revealed rectal stenosis due to Schnitzler's metastasis. When the distance from the distal end of the obstruction to the anal verge is less than 5 cm, stent replacement has been said to be contraindicated due to the development of anal pain and foreign body sensation. Although the distance from the distal end of the obstruction to the anal verge was 4 cm in this case, a WallFlex(TM) colonic stent could be placed. She stayed home, and luminal patency remained until she died 270 days after stent insertion. This report demonstrates that rectal obstruction located less than 5 cm from the anal verge due to Schnitzler's metastasis could be treated by stenting without any symptomatic or technical complications.