High-standard predictive equations for estimating body composition using bioelectrical impedance analysis: a systematic review.

The appropriate use of predictive equations in estimating body composition through bioelectrical impedance analysis (BIA) depends on the device used and the subject's age, geographical ancestry, healthy status, physical activity level and sex. However, the presence of many isolated predictive equations in the literature makes the correct choice challenging, since the user may not distinguish its appropriateness. Therefore, the present systematic review aimed to classify each predictive equation in accordance with the independent parameters used. Sixty-four studies published between 1988 and 2023 were identified through a systematic search of international electronic databases. We included studies providing predictive equations derived from criterion methods, such as multi-compartment models for fat, fat-free and lean soft mass, dilution techniques for total-body water and extracellular water, total-body potassium for body cell mass, and magnetic resonance imaging or computerized tomography for skeletal muscle mass. The studies were excluded if non-criterion methods were employed or if the developed predictive equations involved mixed populations without specific codes or variables in the regression model. A total of 106 predictive equations were retrieved; 86 predictive equations were based on foot-to-hand and 20 on segmental technology, with no equations used the hand-to-hand and leg-to-leg. Classifying the subject's characteristics, 19 were for underaged, 26 for adults, 19 for athletes, 26 for elderly and 16 for individuals with diseases, encompassing both sexes. Practitioners now have an updated list of predictive equations for assessing body composition using BIA. Researchers are encouraged to generate novel predictive equations for scenarios not covered by the current literature.Registration code in PROSPERO: CRD42023467894.

Monitoring Internal Load in Women's Basketball via Subjective and Device-Based Methods: A Systematic Review.

The monitoring of internal load in basketball can be used to understand the effects and potential physiological adaptations caused by external load. The main aim of this systematic review was to identify the methods and variables used to quantify internal load in female basketball. The studies included different populations and events: youth athletes, elite, and amateur players. Subjective methods included using the rating of perceived exertion (RPE) method, and sensor-based methods included monitoring the cardiac response to exercise, using heart rate (HR) as the primary metric. The results showed that the HRAvg exhibited a wider range of values during training than during competition, and different metrics were used to evaluate internal load, such as HRMax, HRmin, %HRMax, total time and % of time spent in different HR zones (2-8 zones), Banister's TRIMP, and summated HR zones. RPE and HR metrics were the most commonly used methods. However, the use of multiple metrics with little standardization resulted in significant heterogeneity among studies, limiting meaningful comparisons. The review provides a reference for current research on female basketball. Future research could address this limitation by adopting more consistent measurement protocols standardizing the use of metrics.

The effect of training schedule and playing positions on training loads and game demands in professional handball players.

In this research, we aimed to (1) describe the differences in internal and external load between playing positions and (2) characterize the training demands of the days before competitive events for professional handball players. Fifteen players (5 wings, 2 centre backs, 4 backs, and 2 pivots) were equipped with a local positioning system device during training and 11 official matches. External (total distance, high-speed running, player load) and internal loads (rating of perceived exertion) were computed. Substantial differences were recorded between the external load variables depending on each playing position and depending on whether it was a training day (high-speed running: effect size (ES) ≥ 2.07; player load: ES ≥ 1.89) or a match (total distance: ES ≥ 1.27; high-speed running: ES ≥ 1.42; player load: ES ≥ 1.33). Differences in internal load were not substantial. The rating of perceived exertion, at this competitive level, does not seem to discriminate the differences registered in the external load, probably due to the degree of adaptation to the specific effort of these players. The large differences observed in external load variables should be used to tailor practices and better adjust the training demands in professional handball settings.

Monitoring external load in elite male handball players depending on playing positions.

Monitoring workload is critical for elite training and competition, as well as preventing potential sports injuries. The assessment of external load in team sports has been provided with new technologies that help coaches to individualize training and optimize their team's playing system. In this study we characterized the physical demands of an elite handball team during an entire sports season. Novel data are reported for each playing position of this highly strenuous body-contact team sport. Sixteen world top players (5 wings, 2 centre backs, 6 backs, 3 line players) were equipped with a local positioning system (WIMU PRO) during fourteen official Spanish first league matches. Playing time, total distance covered at different running speeds, and acceleration variables were monitored. During a handball match, wings cover the greater distance by high-speed running (> 5.0 m·s-1): 410.3 ± 193.2 m, and by sprint (> 6.7 m·s-1): 98.0 ± 75.4 m. Centre backs perform the following playing position that supports the highest speed intensities during the matches: high-speed running: 243.2 ± 130.2 m; sprint: 62.0 ± 54.2 m. Centre backs also register the largest number of high-intensity decelerations (n = 142.7 ± 59.5) compared to wings (n = 112.9 ± 56.0), backs (n = 105.2 ± 49.2) and line players: 99.6 ± 28.9). This study provides helpful information for professional coaches and their technical staff to optimize training load and individualize the physical demands of their elite male handball players depending on each playing position.

The effects of COVID-19 lockdown on jumping performance and aerobic capacity in elite handball players.

The aim of this research was to analyse the capacity of a home-based training programme to preserve aerobic capacity and jumping performance in top-level handball players during the COVID-19 lockdown. Eleven top-level male handball players from the same team participated in the study. A submaximal shuttle run test and a counter-movement jump test were used to measure the players' aerobic fitness and lower limb explosive strength, respectively. A 9-week home-based training programme was followed during lockdown. Pre-test measurements were assessed before the pandemic on 29 January 2020 and ended on 18 May 2020. Moderate significant mean heart rate increases were found in the late stages of the submaximal shuttle run test after the lockdown (stage 5, 8.6%, P = 0.015; ES = 0.873; stage 6, 7.7%, P = 0.020; ES = 0.886; stage 7, 6.4%, P = 0.019; ES = 0.827). Moderate significant blood lactate increases were observed immediately after the submaximal shuttle run test following the lockdown (30.1%, P = 0.016; ES = 0.670). In contrast, no changes were found in jump performance. A structured home-based training programme during the COVID-19 lockdown preserved lower limb explosive strength but was an insufficient stimulus to maintain aerobic capacity in top-level handball players.

Preliminary Results of an Exercise Program After Laparoscopic Resective Colorectal Cancer Surgery in Non-Metastatic Adenocarcinoma: A Pilot Study of a Randomized Control Trial.

Performing physical exercise after a colorectal cancer diagnosis is associated with lower mortality related to the tumor itself. In order to improve physical recovery after elective surgery, there are no specific exercise protocols after discharge from the hospital. The purpose of this study is to show the preliminary results of an exercise program after colorectal cancer surgery. Six patients with non-metastatic colorectal adenocarcinoma addressed to respective laparoscopic were randomly assigned to a mixed supervised/home-based exercise program for six months and compared to a control group without exercise. To assess the effectiveness of the program, functional and body composition parameters were evaluated. Three months after surgery, the exercise group increased flexibility (p < 0.01, ES = 0.33), strength of lower limbs (p < 0.01, ES = 0.42) and aerobic capacity (p < 0.01, ES = 0.28). After surgery, the six patients experienced a significant reduction in body mass index (BMI) and free fat mass. More specifically, fat mass reached the lowest values, with a concomitant increase in cell mass after six months (p < 0.01, ES = 0.33). This did not occur in the control group. Colorectal cancer treatment induces a reduction in physical function, particularly during the first six months after treatment. A mixed exercise approach appears promising in countering this process after colorectal cancer surgery.

Bioelectrical Impedance Vector Analysis (BIVA) and Body Mass Changes in an Ultra-Endurance Triathlon Event.

This study aimed to provide the first description of the whole-body bioimpedance vector of nine non-professional triathletes, and to assess body mass (BM) and vector variations evoked by an ultra-endurance triathlon event. Anthropometric and bioelectrical assessments were performed before (PRE), after (POST), and 48 hours following the race (POST48h). Bioimpedance vector analysis (BIVA) showed triathletes' vectors placed to the left of the major axis and mostly outside the 50% tolerance ellipse of the reference population. Vector migration in POST indicated dehydration, paralleled by a decrease in BM (p = 0.0001). Increased hydration status from POST to POST48h was suggested by a reversed vector migration and increased BM (p = 0.0001). Compared to PRE, POST48h values reflected fluid retention by changes in BIVA, while BM was still lower (p = 0.0001). Racing time was positively related to basal resistance -R/h- (r = 0.68; p = 0.04) and bioimpedance -Z/h- (r = 0.68; p = 0.045). Besides, basal R/h and Z/h were positively related to PRE-to-POST changes of R/h and Z/h (r = 0.80; p = 0.009). PRE-to-POST changes of R/h and Z/h were positively related to racing time (r = 0.80, p = 0.01) and internal workload (r = 0.80, p ≤ 0.02). Notwithstanding the lack of significant correlation between BM and bioelectrical parameters, the vector's behavior was explained from a multifactorial perspective (including BM variations) by using multiple regression analysis. On the other hand, BM changes were not related to racing time, internal workload or energy deficit (ranges: r = - 0.46 to 0.65; p = 0.06 to 0.98). In conclusion, these triathletes exhibit a specific bioelectrical distribution. Furthermore, vector migration was consistent with fluid loss induced by the event. Finally, vector analysis seems to provide additional information about hydration changes 48h after the event in comparison with BM alone.

Effects of acute beetroot juice intake on performance, maximal oxygen uptake, and ventilatory efficiency in well-trained master rowers: a randomized, double-blinded crossover study.

BACKGROUND: Beetroot juice (BRJ) intake has been considered a practical nutritional strategy among well-trained athletes. This study aimed to assess the effects of BRJ intake on performance, cardiorespiratory and metabolic variables during a simulated 2000-meter rowing ergometer test in well-trained master rowers. METHOD: Ten well-trained male master rowers (30-48 years) participated in a randomized, double-blind, crossover design for 3 weeks. In the first week, a researcher explained all the experimental procedures to the participants. In the next two weeks, the participants were tested in 2 rowing ergometer sessions, separated from each other by a 7-day washout period. In both strictly identical sessions, the participants randomly drank BRJ or placebo (PL) 3 hours before the start of the tests. Subsequently, the participants carried out the 2000-meter rowing ergometer tests. Oxygen saturation and blood lactate measurements were performed before starting (pretest) and at the end of the test (posttest). Performance parameters and cardiorespiratory variables were recorded during the rowing ergometer test. RESULTS: An improvement in time trial performance was observed, with a mean difference of 4 seconds (90% confidence limits ± 3.10; p ≤ 0.05) compared to PL. Relative and absolute maximaloxygenuptakeV˙O2max increased (mean difference of 2.10 mL·kg-1·min-1, 90% confidence limits ± 1.80; mean difference of 0.16 L·min-1 90% confidence limits ± 0.11, respectively; p ≤ 0.05) compared to PL. No ergogenic effect was observed on ventilatory efficiency and blood lactate concentrations after BRJ intake. CONCLUSION: Acute BRJ intake may improve time trial performance as well as V˙O2max in well-trained master rowers. However, BRJ does not appear to improve ventilatory efficiency.

Bioelectrical, Anthropometric, and Hematological Analysis to Assess Body Fluids and Muscle Changes in Elite Cyclists during the Giro d'Italia.

This study aimed to characterize and monitor the body fluid and muscle changes during the Giro d'Italia in nine elite cyclists via bioelectrical (whole-body and muscle-localized) anthropometric and hematological analysis. There were three checkpoint assessments: at the beginning, middle, and end of the race. The Wilcoxon signed-rank test was used to compare the data at baseline and follow up. The Spearman correlation was used to explore relationships between variables. Hotelling's T2 test was used to determine bioelectrical differences in the complex vector. Bodyweight did not change during the competition, despite bioelectrical and hematological data indicating that at the first half of the race, there was a fluid gain, and in the second half a fluid loss occurred, reaching baseline values. These changes were especially prevalent in the extracellular water compartment. Significant correlations between whole-body bioelectrical vector changes and red blood cell parameter changes were reported. The muscle group most sensitive to changes were the calves. Quadriceps, hamstrings, and calves reported a PhA decrease trend during the first half of the race, and an increase during the second half. Bioelectrical impedance vector analysis appears to be sensitive enough to detect hydration and cellular integrity adaptions induced by competitions as demanding as the Giro d'Italia.

Physiological demands and motion analysis of elite foil fencing.

The aim of this study was to determine the physiological demands and motion analysis of a simulated fencing competition. Eighteen fencers each completed 5 'poule' (5 touches) and 'direct elimination (DE)' (15 touches) fights. Expired gases were measured during 1 poule and DE fight. Heart rate (HR), ratings of perceived exertion (RPE) and movement data were recorded throughout all fights. Motion analysis was undertaken using the software LINCE PLUS. Differences between poule and DE fights were determined by either a paired t-test or a one-way repeated measures ANOVA. HR and RPE were significantly greater during DE compared to poule (170 ±10 vs 163 ±13 beats·min-1; P<0.05). A greater distance was covered during a DE fight compared to a poule fight (459.9 ± 117.7 m vs 162.6 ± 74.2 m; P<0.05). The average values of [Formula: see text] were 42.5 ±5.6 ml·kg-1·min-1 in men and 34.4 ±3.2 ml·kg-1·min-1 in women. Work-to-rest ratios reduced during the DE fights along with a lower average speed and increased max speed (11.7 ± 2.8 km∙h-1 vs 9.6 ± 1.6 km∙h-1; P<0.05). In conclusion, there is an increased physiological demand during a DE fight accompanied by a reduction in average speed and decreased work-to-rest ratio.

Body fluids and muscle changes in trail runners of various distances.

Background: This study aims to investigate body fluids and muscle changes evoked by different trail races using anthropometric, bioelectrical, and creatine kinase (CK) measurements. Methods: A total of 92 subjects (55 men, 37 women) participating in three different races of 14, 35, and 52 km were evaluated before (PRE) and after (POST) the races. Classic bioelectrical impedance vector analysis was applied at the whole-body level (WB-BIVA). Additionally, muscle-localized bioelectrical assessments (ML-BIVA) were performed in a subgroup of 11 men (in the quadriceps, hamstrings, and calves). PRE-POST differences and correlations between bioelectrical values and CK, running time and race distance were tested. Results: Changes in whole-body vectors and phase angles disclosed an inclination towards dehydration among men in the 14, 35, and 52 km groups (p < 0.001), as well as among women in the 35 and 52 km groups (p < 0.001). PRE Z/H was negatively correlated with running time in the 35 km men group and 14 km women group (r = -0.377, p = 0.048; r = -0.751, p = 0.001; respectively). POST Z/H was negatively correlated with running time in the 14 km women group (r = -0.593, p = 0.02). CK was positively correlated with distance in men and women (p < 0.001) and negatively correlated with reactance and vector length in the 14 km men group (p < 0.05). ML-BIVA echoed the same tendency as the WB-BIVA in the 35 and 52 km runners, with the most notable changes occurring in the calves (p < 0.001). Conclusions: WB-BIVA and CK measurements underscored a conspicuous trend towards post-race dehydration and muscle damage, displaying a weak association with performance. Notably, ML-BIVA detected substantial alterations primarily in the calves. The study underscores the utility of BIVA as a technique to assess athlete's body composition changes.

Correction: Cebrián-Ponce et al. Bioelectrical, Anthropometric, and Hematological Analysis to Assess Body Fluids and Muscle Changes in Elite Cyclists during the Giro d'Italia. Biology 2023, 12, 450.

In the original publication [...].

The bioelectrical impedance analysis (BIA) international database: aims, scope, and call for data.

BACKGROUND: Bioelectrical impedance analysis (BIA) is a technique widely used for estimating body composition and health-related parameters. The technology is relatively simple, quick, and non-invasive, and is currently used globally in diverse settings, including private clinicians' offices, sports and health clubs, and hospitals, and across a spectrum of age, body weight, and disease states. BIA parameters can be used to estimate body composition (fat, fat-free mass, total-body water and its compartments). Moreover, raw measurements including resistance, reactance, phase angle, and impedance vector length can also be used to track health-related markers, including hydration and malnutrition, and disease-prognostic, athletic and general health status. Body composition shows profound variability in association with age, sex, race and ethnicity, geographic ancestry, lifestyle, and health status. To advance understanding of this variability, we propose to develop a large and diverse multi-country dataset of BIA raw measures and derived body components. The aim of this paper is to describe the 'BIA International Database' project and encourage researchers to join the consortium. METHODS: The Exercise and Health Laboratory of the Faculty of Human Kinetics, University of Lisbon has agreed to host the database using an online portal. At present, the database contains 277,922 measures from individuals ranging from 11 months to 102 years, along with additional data on these participants. CONCLUSION: The BIA International Database represents a key resource for research on body composition.

Physical Fitness and Performance in Talented & Untalented Young Chinese Soccer Players.

Sports performance is a complex process that involves many factors, including ethnic and racial differences. China's youth soccer is in a process of constant development, although information about the characteristics of its players and their methodological systems is scarce. The aim of this retrospective study was to characterize the physical fitness and the competitive performance of 722 Chinese players of three sports categories (8.0-9.9, 10.0-11.9 and 12.0-13.9 years), who were classified by their coaches as talented (n = 204) or untalented (n = 518). Players were assessed for anthropometry (body height, body mass, body mass index), lung capacity (Forced Vital Capacity), jumping performance (Squat Jump, Countermovement Jump and Abalakov tests), sprinting performance (10 m and 30 m Sprint tests), agility performance (Repeated Side-Step test) and flexibility (Sit & Reach test). A descriptive, comparative, correlational and multivariate analysis was performed. Competitive ranking was created in order to act as dependent variable in multiple linear regression analysis. Results indicate that Chinese players classified as talented have better motor performance than untalented ones. However, these differences are neither related nor determine the competitive performance of one group or the other.

Segmental body composition estimated by specific BIVA and dual-energy X-ray absorptiometry.

AIMS: The aim of this study was to analyse the association between specific bioelectric impedance vector analysis (BIVA) and dual-energy X-ray absorptiometry (DXA) to assess segmental body composition using DXA as the reference technique. METHODS: The sample comprised 50 young active students who practised or played different sports (25 men, age: 24.37 ± 4.79 y; 25 women, age: 24.32 ± 4.43 y) from the National Institute of Physical Education of Catalonia (INEFC). Anthropometric data (height, weight, arm, waist, and calf circumferences) and bioelectrical measurements (R, ohm; Xc, ohm) were recorded. Body composition was analysed with specific BIVA. DXA was used as the reference method to assess body composition of the whole-body, the trunk, and the limbs. The percentage of fat mass (%FMDXA) and fat-free mass index (FFMIDXA = FFM/length2) were calculated. The agreement between specific BIVA and DXA was evaluated by a depth-depth analysis, two-way ANOVA, and Pearson's correlations. RESULTS: The depth-depth analysis showed a good agreement between DXA and BIVA (F = 14.89, p < 0.001) in both sexes and all body segments. Specific vector length (Zsp; i.e. indicative of %FM) was correlated with %FMDXA in the whole body and all body segments, and the phase angle was correlated with FFMIDXA, with he trunk in women as the only exception. Specific BIVA demonstrated to balance the effect of body size on bioelectrical measurements in both whole and segmental approaches. CONCLUSIONS: Segmental specific BIVA and DXA provided a consistent evaluation of body composition in both sexes, of the whole body and each body segment. The indices %FM and FFMI obtained with DXA were correlated to vector length and phase angle in each segment, respectively. Specific BIVA represents a promising technique for monitoring segmental body composition changes in sport science and clinical applications.

Electrical Impedance Myography in Health and Physical Exercise: A Systematic Review and Future Perspectives.

Background: Electrical impedance myography (EIM) is a non-invasive method that provides information about muscle health and changes that occur within it. EIM is based on the analysis of three impedance variables: resistance, reactance, and the phase angle. This systematic review of the literature provides a deeper insight into the scope and range of applications of EIM in health and physical exercise. The main goal of this work was to systematically review the studies on the applications of EIM in health and physical exercise in order to summarize the current knowledge on this method and outline future perspectives in this growing area, including a proposal for a research agenda. Furthermore, some basic assessment principles are provided. Methods: Systematic literature searches on PubMed, Scopus, SPORTDiscus and Web of Science up to September 2020 were conducted on any empirical investigations using localized bioimpedance devices to perform EIM within health and physical exercise contexts. The search included healthy individuals, elite soccer players with skeletal muscle injury, and subjects with primary sarcopenia. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist was used to develop the systematic review protocol. The quality and risk of bias of the studies included were assessed with the AQUA tool. Results: Nineteen eligible original articles were included in this review, which were separated into three tables according to the nature of the study. The first table includes six studies on the bioelectrical characterization of muscle. The second table includes five studies analyzing muscle changes in injured elite soccer players. The third table includes studies on the short-, medium-, and long-term bioelectrical adaptations to physical exercise. Conclusions: EIM has been used for the evaluation of the muscle condition in the clinical field over the last few years, especially in different neuromuscular diseases. It can also play an important role in other contexts as an alternative to complex and expensive methods such as magnetic resonance imaging. However, further research is needed. The main step in establishing EIM as a valid tool in the scientific field is to standardize the protocol for performing impedance assessments.

Bioelectrical impedance vector analysis (BIVA) in renal transplant recipients during an unsupervised physical exercise program.

BACKGROUND: Muscle weakness, incorrect body water distribution and reduced exercise tolerance are the main characteristics found in renal transplant recipients after surgical treatment. Regular physical supervised exercise programs, at moderate intensity, have been promoted to contrast these aspects, while few data are available for long-term unsupervised mixed exercise plans. Bioelectrical impedance vector analysis (BIVA) provides a semi-quantitative evaluation of body cell mass and body water. This study aims to approach the role of the BIVA analysis in the follow-up of Renal Transplant Group (RTG) and analyze the impact of unsupervised exercise program. METHODS: Thirteen male RTG and ten healthy subjects, adherent to a tailored exercise program, at moderate intensity and prescribed in an unsupervised way, have been followed up for one year. Every six months all the subjects have been submitted to the ergometric test, echocardiographic exam and an analysis of body composition by bioimpedance. They were compared to a healthy control group (HG). RESULTS: A significant reduction of the BMI was observed at the end of the study in the RTG group (T0 24.8±3.2, T12 24.2±3.2 kg/m2; P<0.05). BIVA has shown a lower right quadrant for RTG. All echocardiographic parameters were in a normal range, and no differences were found over time. CONCLUSIONS: Unsupervised tailored and mixed exercise intervention reduces some cardiovascular risks factors. However, it does not modify the frailty of RTG. BIVA analysis seems to have appropriate sensitivity to highlight this aspect.

Bioelectrical impedance vector analysis (BIVA) in sport and exercise: Systematic review and future perspectives.

BACKGROUND: Bioelectrical impedance vector analysis (BIVA) is a general concept that includes all methodologies used in the analysis of the bioelectrical vector, whereas the "classic" BIVA is a patented methodology included among these methods of analysis. Once this was clarified, the systematic review of the literature provides a deeper insight into the scope and range of application of BIVA in sport and exercise. OBJECTIVE: The main goal of this work was to systematically review the sources on the applications of BIVA in sport and exercise and to examine its usefulness and suitability as a technique for the evaluation of body composition, hydration status, and other physiological and clinical relevant characteristics, ultimately to trace future perspectives in this growing area, including a proposal for a research agenda. METHODS: Systematic literature searches in PubMed, SPORTDiscus and Scopus databases up to July, 2017 were conducted on any empirical investigations using phase-sensitive bioimpedance instruments to perform BIVA within exercise and sport contexts. The search included healthy sedentary individuals, physically active subjects and athletes. RESULT: Nineteen eligible papers were included and classified as sixteen original articles and three scientific conference communications. Three studies analysed short-term variations in the hydration status evoked by exercise/training through whole-body measurements, eleven assessed whole-body body composition changes induced by long-term exercise, four compared athletic groups or populations using the whole-body assessment, and two analysed bioelectrical patterns of athletic injuries or muscle damage through localised bioimpedance measurements. CONCLUSIONS: BIVA is a relatively new technique that has potential in sport and exercise, especially for the assessment of soft-tissue injury. On the other hand, the current tolerance ellipses of "classic" BIVA are not a valid method to identify dehydration in individual athletes and a new approach is needed. "Specific" BIVA, a method which proposes a correction of bioelectrical values for body geometry, emerges as the key to overcome "classic" BIVA limitations regarding the body composition assessment. Further research establishing standardised testing procedures and investigating the relationship between physiology and the bioelectrical signal in sport and exercise is needed.

Assessing subcutaneous adipose tissue by simple and portable field instruments: Skinfolds versus A-mode ultrasound measurements.

PURPOSE: This study compared subcutaneous adipose tissue (SAT) measurements using a skinfold caliper and Renco Lean-Meater Series 12 A-mode portable ultrasound scanner (A-US). It aimed to assess their inter- and intra-rater reliability and measure the agreement between both methods. METHODS: Eighty-four volunteers of different fitness levels were divided into three groups by Æ©6 skinfolds: G1 ≤ 55 mm (n = 33 males); G2 > 55 mm (n = 32 males); G3 = 98.0 ± 52.3 mm (n = 19 females). Triceps, subscapular, biceps, iliac crest, supraspinal, abdominal, front thigh and medial calf were assessed by ultrasound and skinfolds. Two technicians for both tools performed triplicate measures. Intraclass correlation (ICC), technical error of measurement (TEM) and coefficients of variation (CVs) were applied for test-retest and inter-rater reliability. Non-Parametric statistics were used in order to establish possible statistical differences and correlation between skinfolds thickness and uncompressed subcutaneous adipose tissue thickness from ultrasound. The amount of agreement between both methods was assessed with Lin's coefficient and a scatterplot of all site locations. A Bland-Altman plot was constructed to establish limits of agreement between groups and regression analysis was employed to assess the ability of skinfolds to explain the variance of ultrasound. RESULTS: Test-retest ICC for skinfolds and ultrasound were higher than 0.989 and 0.793, respectively. Inter-rater ICC for skinfolds was 0.999 with a 95% CI of 0.995 to 0.999 and for ultrasound was 0.755 with a much larger 95% CI of 0.622 to 0.841. TEMs (> 8.50%) and CVs (> 6.72%) compromised ultrasound reliability. Statistical differences were found in most of the analysed anatomical sites (p < 0.001) except in biceps G2 (Z = -1.150, p = 0.25) and G3 (Z = -1.309, p = 0.19). Good correlations (r > 0.7, p ≤ 0.05) were reported at almost all anatomical sites and groups except for biceps (G1: Rho = 0.26, p = 0.140) and abdominal (G2: Rho = -0.16, p = 0.38; G3: Rho = 0.43, p = 0.068). Lin's concordance correlation coefficient registered low values of agreement between skinfolds and A-mode ultrasound (ranged from-0.009-0.646). The scatterplot and the estimated regression line drawn through the midst of all anatomical sites of the whole sample had a slope of 0.51 and R2 adjusted = 0.62 was obtained. The combined analysis between the Bland-Altman plot and the linear regression showed that specifically in the G2 and G3 groups, as the SAT increases the differences between skinfolds and ultrasounds measurements also increases. CONCLUSIONS: The Renco Lean-Meater ultrasound is not interchangeable with skinfold measures. Its utility is questionable, particularly for assessing SAT in active adult populations. Its poor test-retest and inter-rater reliability as well as the lack of agreement when compared to the skinfolds would exclude the free use of the A-mode ultrasound scanner in its hypothetical replacing of the classical calipers.

Differences between the sexes in athletes' body composition and lower limb bioimpedance values.

INTRODUCTION: The differences between the sexes in body composition are well established, although sports activity at an elite level seems to reduce them. The aim of this study is a comparison of the sexes in terms of athletes' body composition with a three-compartment model and a localized bioimpedance analysis in elite soccer players. METHODS: 18 female and 18 male elite soccer players of the same age were matched (female=26.2±2.4, male 26.9±2.5; p=0.87). An assessment of body composition was performed through the integration of anthropometrics parameters, skinfold thickness and bioimpedance data. The evaluations were carried out in the morning on a group of athletes who were in a rested condition, having not exercised in the previous 12 hours and having fasted for breakfast. RESULTS: A body composition assessment shows higher values in females for hip circumference/ height (female: 0.55±0.03, male: 0.52±0.02; p<0.01) and fat mass index (female: 3.7±0.7 kg/m2, male: 2.4±0.4 kg/m2; p<0.001), while there is no difference between the genders in the extra cellular mass index (female: 7.1±1.2 kg/m2, male: 7.6±0.4 kg/m2; p=0.11). A localized bioimpedance analysis describes well-defined differences in the thighs, while in the calves these differences are reversed for the reactance values. CONCLUSION: Sports activity seems to slightly reduce differences in the whole body. The different adaption at the same physical effort appears to be mainly related to cellular mass. This study shows for the first time localized bioimpedance values in female athletes. LEVEL OF EVIDENCE: II b.