RESUMEN
Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal antibody. In study 1, hepatic BROD, EROD, and PROD activities were significantly induced in DCCO orally exposed to 20ml/kg body weight (bw) oil as a single dose or daily for 5 days. Western blot analysis revealed hepatic CYP1A protein induction in both treatment groups. In study 2 (5ml/kg bw oil or 10ml/kg bw oil, 21day exposure), all four hepatic ARODs were significantly induced. Western blots showed an increase in hepatic CYP1A expression in both treatment groups with a significant induction in birds exposed to 10ml/kg oil. Significant correlations were detected among all 4 AROD activities in both studies and between CYP1A protein expression and both MROD and PROD activities in study 2. EROD activity was highest for both treatment groups in both studies while BROD activity had the greatest fold-induction. While PROD activity values were consistently low, the fold-induction was high, usually 2nd highest to BROD activity. The observed induced AROD profiles detected in the present studies suggest both CYP1A4/1A5 DCCO isoforms are being induced after MC252 oil ingestion. A review of the literature on avian CYP1A AROD activity levels and protein expression after exposure to CYP1A inducers highlights the need for species-specific studies to accurately evaluate avian exposure to oil.
RESUMEN
Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal antibody. In study 1, hepatic BROD, EROD, and PROD activities were significantly induced in DCCO orally exposed to 20ml/kg body weight (bw) oil as a single dose or daily for 5 days. Western blot analysis revealed hepatic CYP1A protein induction in both treatment groups. In study 2 (5ml/kg bw oil or 10ml/kg bw oil, 21day exposure), all four hepatic ARODs were significantly induced. Western blots showed an increase in hepatic CYP1A expression in both treatment groups with a significant induction in birds exposed to 10ml/kg oil. Significant correlations were detected among all 4 AROD activities in both studies and between CYP1A protein expression and both MROD and PROD activities in study 2. EROD activity was highest for both treatment groups in both studies while BROD activity had the greatest fold-induction. While PROD activity values were consistently low, the fold-induction was high, usually 2nd highest to BROD activity. The observed induced AROD profiles detected in the present studies suggest both CYP1A4/1A5 DCCO isoforms are being induced after MC252 oil ingestion. A review of the literature on avian CYP1A AROD activity levels and protein expression after exposure to CYP1A inducers highlights the need for species-specific studies to accurately evaluate avian exposure to oil.
RESUMEN
From September to November 2000, United States Fish and Wildlife Service biologists investigated incidents involving 221 bird deaths at 3 mine sites located in New Mexico and Arizona. These bird deaths primarily involved passerine and waterfowl species and were assumed to be linked to consumption of acid metalliferous water (AMW). Because all of the carcasses were found in or near pregnant leach solution ponds, tailings ponds, and associated lakes or storm water retention basins, an acute-toxicity study was undertaken using a synthetic AMW (SAMW) formulation based on the contaminant profile of a representative pond believed to be responsible for avian mortalities. An acute oral-toxicity trial was performed with a mixed-sex group of mallards (Anas platyrhynchos). After a 24-h pretreatment food and water fast, gorge drinking was evident in both SAMW treatment and control groups, with water consumption rates greatest during the initial drinking periods. Seven of nine treated mallards were killed in extremis within 12 h after the initiation of dose. Total lethal doses of SAMW ranged from 69.8 to 270.1 mL/kg (mean ± SE 127.9 ± 27.1). Lethal doses of SAMW were consumed in as few as 20 to 40 min after first exposure. Clinical signs of SAMW toxicity included increased serum uric acid, aspartate aminotransferase, creatine kinase, potassium, and P levels. PCV values of SAMW-treated birds were also increased compared with control mallards. Histopathological lesions were observed in the esophagus, proventriculus, ventriculus, and duodenum of SAMW-treated mallards, with the most distinctive being erosion and ulceration of the kaolin of the ventriculus, ventricular hemorrhage and/or congestion, and duodenal hemorrhage. Clinical, pathological, and tissue-residue results from this study are consistent with literature documenting acute metal toxicosis, especially copper (Cu), in avian species and provide useful diagnostic profiles for AMW toxicity or mortality events. Blood and kidney Cu concentrations were 23- and 6-fold greater, respectively, in SAMW mortalities compared with controls, whereas Cu concentrations in liver were not nearly as increased, suggesting that blood and kidney concentrations may be more useful than liver concentrations for diagnosing Cu toxicosis in wild birds. Based on these findings and other reports of AMW toxicity events in wild birds, we conclude that AMW bodies pose a significant hazard to wildlife that come in contact with them.
Asunto(s)
Patos , Metales Pesados/toxicidad , Metales Ligeros/toxicidad , Contaminantes Químicos del Agua/toxicidad , Administración Oral , Animales , Conducta Animal/efectos de los fármacos , Ingestión de Líquidos/efectos de los fármacos , Conducta de Ingestión de Líquido/efectos de los fármacos , Monitoreo del Ambiente/métodos , Femenino , Tracto Gastrointestinal/efectos de los fármacos , Tracto Gastrointestinal/patología , Concentración de Iones de Hidrógeno , Riñón/efectos de los fármacos , Riñón/patología , Longevidad/efectos de los fármacos , Masculino , Metales Pesados/química , Metales Ligeros/química , Ácido Nítrico/química , Úlcera Gástrica/inducido químicamente , Úlcera Gástrica/patología , Pruebas de Toxicidad AgudaRESUMEN
Sensitivities of a wildlife species, deer mice, to oxidants were evaluated. A single dose (1589 mg/kg body weight by intraperitoneal injection) of carbon tetrachloride, a typical hepatotoxicant, caused changes in GCL activity and GSH content in multiple organs of deer mice. Hepatic GCL activity and GSH content were depleted substantially (P<0.01), renal GCL activity increased (P<0.05). Blood, brain and heart GCL activities increased (P<0.05), whereas GSH contents decreased significantly. Deer mice were exposed to Pb, or Pb together with Cu and Zn via drinking water for 4 weeks. GCL activities were not significantly affected by treatments. GSH contents were increased significantly by Pb alone, Pb with medium and high concentrations of Cu and Zn. Effects of multi-metal-contaminated soil were investigated via lactational, juvenile and lifelong exposure to feed supplemented with soils. Metal-contaminated soils did not lead to significant effects in pups via lactation, 50-day exposure altered GSH content marginally, while 100-day exposure resulted in marked GCL activity depletion. After 100-day exposure, GCL activities of the medium soil-, high soil- and Pb-treated deer mice were only 53%, 40% and 46% of the control, respectively (P<0.0001).
Asunto(s)
Tetracloruro de Carbono/toxicidad , Glutamato-Cisteína Ligasa/metabolismo , Glutatión/metabolismo , Metales Pesados/toxicidad , Oxidantes/toxicidad , Estrés Oxidativo/efectos de los fármacos , Contaminantes del Suelo/toxicidad , Envejecimiento , Animales , Encéfalo/efectos de los fármacos , Encéfalo/enzimología , Tetracloruro de Carbono/administración & dosificación , Cobre/toxicidad , Femenino , Glutamato-Cisteína Ligasa/sangre , Glutatión/sangre , Inyecciones Intraperitoneales , Riñón/efectos de los fármacos , Riñón/enzimología , Plomo/toxicidad , Hígado/efectos de los fármacos , Hígado/enzimología , Masculino , Miocardio/enzimología , Oxidantes/administración & dosificación , Peromyscus , Medición de Riesgo , Zinc/toxicidadRESUMEN
Glutamate cysteine ligase (GCL), synthesizing gamma-glutamylcysteine from glutamate and cysteine, is the rate-limiting enzyme in glutathione (GSH) biosynthesis. GCL activity measurement was optimized in tissues from deer mice, Sprague Dawley rats, and mallard ducks. Varying glutamic acid concentrations from 5 to 80 mM did not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influenced GCL activities substantially. Optimal cysteine concentrations for deer mouse, Sprague Dawley rat, and mallard duck (respectively) were 30, 30, and 20 mM in liver, 10, 10, and 20 mM in kidney, 20, 20, and 30 mM in brain, and 30 mM in heart for all three species. Responses of mallard duck GCL activity to acid metalliferous water were evaluated. After subacute exposure, low doses increased GCL activity and GSH content in liver by 48.3% and 54.4%, respectively. High doses reduced GCL activities significantly in liver and kidney to 31.2% and 43.0% of the control, respectively.
Asunto(s)
Animales Salvajes/metabolismo , Monitoreo del Ambiente/métodos , Contaminantes Ambientales/toxicidad , Glutamato-Cisteína Ligasa/metabolismo , Metales/toxicidad , Animales , Relación Dosis-Respuesta a Droga , Patos , Contaminantes Ambientales/farmacocinética , Glutatión/biosíntesis , Metales/farmacocinética , Ratones , Ratas , Ratas Sprague-Dawley , Distribución TisularRESUMEN
Perchlorate, the oxidizer component in most solid rocket propellant formulations, is known to inhibit the uptake of iodide into the thyroid gland, thereby reducing production of the thyroid hormones, triiodothyronine and thyroxine (T4). Thyroid hormones regulate metabolism in endothermic organisms and are responsible for maintenance of homeothermic body temperatures. Little is known about the effects of perchlorate on metabolic capacity. The objectives of the present study were to determine if subchronic (51 d; 0, 1, and 10 mg/kg/d) and chronic (180 d; 0.75 mg/kg/d) perchlorate exposure in adult male prairie voles (Microtus ochrogaster) would alter resting metabolic rates as a result of decreased circulating thyroid hormone concentrations and to determine if perchlorate exposure disrupts thermogenesis in mammals exposed to cold stress. Voles exposed to perchlorate for 51 or 180 d experienced no significant alterations in resting metabolic rates at any point during the exposure period. Additionally, the treatment had no effect on peak metabolic rates or plasma thyroid hormone concentrations. However, thyroid gland T4 concentrations were significantly lower in perchlorate-exposed voles than in controls, indicating that thyroid gland T4 content may be a more sensitive endpoint than other thyroid variables for assessing perchlorate exposure. Overall, the present study did not provide evidence for energetic alterations associated with perchlorate exposure at concentrations that are higher than those typically found in groundwater or surface water in the environment.