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1.
Int J Med Sci ; 18(3): 652-659, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33437200

RESUMEN

Objective: Different anesthetics have distinct effects on the interstitial fluid (ISF) drainage in the extracellular space (ECS) of the superficial rat brain, while their effects on ISF drainage in the ECS of the deep rat brain still remain unknown. Herein, we attempt to investigate and compare the effects of propofol and isoflurane on ECS structure and ISF drainage in the caudate-putamen (CPu) and thalamus (Tha) of the deep rat brain. Methods: Adult Sprague-Dawley rats were anesthetized with propofol or isoflurane, respectively. Twenty-four anesthetized rats were randomly divided into the propofol-CPu, isoflurane-CPu, propofol-Tha, and isoflurane-Tha groups. Tracer-based magnetic resonance imaging (MRI) and fluorescent-labeled tracer assay were utilized to quantify ISF drainage in the deep brain. Results: The half-life of ISF in the propofol-CPu and propofol-Tha groups was shorter than that in the isoflurane-CPu and isoflurane-Tha groups, respectively. The ECS volume fraction in the propofol-CPu and propofol-Tha groups was much higher than that in the isoflurane-CPu and isoflurane-Tha groups, respectively. However, the ECS tortuosity in the propofol-CPu and propofol-Tha groups was much smaller than that in isoflurane-CPu and isoflurane-Tha groups, respectively. Conclusions: Our results demonstrate that propofol rather than isoflurane accelerates the ISF drainage in the deep rat brain, which provides novel insights into the selective control of ISF drainage and guides selection of anesthetic agents in different clinical settings, and unravels the mechanism of how general anesthetics function.


Asunto(s)
Anestésicos Generales/administración & dosificación , Núcleo Caudado/efectos de los fármacos , Líquido Extracelular/metabolismo , Putamen/efectos de los fármacos , Tálamo/efectos de los fármacos , Administración por Inhalación , Animales , Núcleo Caudado/citología , Núcleo Caudado/diagnóstico por imagen , Núcleo Caudado/metabolismo , Espacio Extracelular/efectos de los fármacos , Espacio Extracelular/metabolismo , Gadolinio DTPA/administración & dosificación , Infusiones Parenterales , Isoflurano/administración & dosificación , Imagen por Resonancia Magnética/métodos , Modelos Animales , Propofol/administración & dosificación , Putamen/citología , Putamen/diagnóstico por imagen , Putamen/metabolismo , Ratas , Ratas Sprague-Dawley , Tálamo/citología , Tálamo/diagnóstico por imagen , Tálamo/metabolismo
2.
J Cell Biochem ; 120(3): 2859-2868, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-29232010

RESUMEN

OBJECTIVE: To clarify the mechanism of heat shock protein 27 (HSP27) as a diagnostic biomarker in coronary heart disease (CHD) and atherosclerosis (AS). METHOD: Expressions of HSP27 in patients with CHD and healthy controls were determined by enzyme-linked immunosorbent assay and the expressions of HSP27 in aortas of patients with CHD and healthy controls were measured by immunohistochemistry. Receiver operating characteristic curve was applied to assess the diagnostic performance of HSP27 in CHD. ApoE-/- mice were included and accordingly grouped. The expressions of HSP27 in AS plaque were measured by quantitative real-time polymerase chain reaction, immunohistochemistry, and Western blot analysis. AS plaque was observed using hematoxylin and eosin staining. DHE was used to detect reactive oxygen species (ROS) levels in aortas. The expressions of mitochondrial apoptosis-related proteins were measured by Western blot analysis. Cell apoptosis was determined by TUNEL staining. RESULTS: HSP27 was highly expressed in patients with CHD than in healthy controls ( P < 0.01). In comparison to the normal group, the model group had increased the relative positive area of HSP27 and higher expressions of HSP27, Bax, caspase-3, and apoptosis index (AI) but decreased Bcl-2 expression in AS plaque, as well as larger plaque areas and elevated ROS levels in the aorta (all P < 0.05). The HSP27-small interfering RNA group had increased expressions of Bax, caspase-3, and AI but decreased Bcl-2 and HSP27 expressions in AS plaque, as well as larger plaque areas, the relative positive area of HSP27 and higher ROS levels in aorta when compared with those in the model group (all P < 0.05). CONCLUSION: HSP27 exerts its protective role by suppressing ROS and AS progression by inhibiting mitochondria apoptosis pathway in CHD.


Asunto(s)
Aterosclerosis/metabolismo , Cardiotónicos/metabolismo , Enfermedad Coronaria/metabolismo , Proteínas de Choque Térmico HSP27/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Animales , Apoptosis , Aterosclerosis/sangre , Estudios de Casos y Controles , Enfermedad Coronaria/sangre , Enfermedad Coronaria/diagnóstico , Femenino , Proteínas de Choque Térmico HSP27/sangre , Humanos , Masculino , Ratones , Persona de Mediana Edad , Mitocondrias/metabolismo , Placa Aterosclerótica/sangre , Placa Aterosclerótica/patología , Curva ROC
3.
J Dairy Sci ; 102(7): 5922-5932, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31030915

RESUMEN

Exopolysaccharide (EPS) of Lactobacillus plantarum WLPL04 and its sulfated EPS were systematically investigated for their antioxidant activities and effects on protecting the oxidative damage of Caco-2 cells from H2O2. Exopolysaccharide was successfully sulfonated from purified EPS as confirmed by Fourier-transform infrared spectroscopy, and the degree of sulfonation was 0.30. Both EPS and sulfated EPS showed antioxidant activities in vitro determined by 1,1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl radical scavenging tests, and those activities of sulfated EPS were significantly enhanced at 1,000 µg/mL. Cell viabilities of Caco-2 in the range of 1 to 100 µg/mL of EPS and sulfated EPS showed no significant difference. In H2O2-damaged Caco-2 cells models, EPS and sulfated EPS significantly inhibited the enhancement of reactive oxygen species and malondialdehyde levels, and sulfated EPS enhanced the effects by 40.86% and 61.11% when compared with the purified EPS at the same concentration of 100 µg/mL, respectively. For the activities of antioxidant-related enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and expression of genes (SOD2, GPX2, MT1M) on Caco-2 cells, strong protection abilities against the oxidative stress were displayed from both EPS and sulfated EPS, and sulfated EPS exhibited significant enhancement as compared with either EPS or control groups. In summary, sulfonation is an effective strategy for improving the antioxidant activities of EPS from L. plantarum WLPL04 in vitro and on Caco-2 cells.


Asunto(s)
Antioxidantes/farmacología , Lactobacillus plantarum/química , Estrés Oxidativo/efectos de los fármacos , Polisacáridos Bacterianos/farmacología , Sustancias Protectoras/farmacología , Antioxidantes/química , Compuestos de Bifenilo/química , Células CACO-2 , Supervivencia Celular/efectos de los fármacos , Humanos , Radical Hidroxilo/química , Mucosa Intestinal/efectos de los fármacos , Lactobacillus plantarum/aislamiento & purificación , Picratos/química , Polisacáridos Bacterianos/química , Sustancias Protectoras/química , Superóxidos/química
4.
Zhonghua Yi Xue Za Zhi ; 92(6): 408-10, 2012 Feb 14.
Artículo en Zh | MEDLINE | ID: mdl-22490903

RESUMEN

OBJECTIVE: To evaluate the efficacy of a 300 mg loading dose of clopidogrel followed by 150 mg as maintenance dose in patients with percutaneous coronary intervention (PCI). METHODS: A total of 108 consecutive patients undergoing elective PCI were recruited from our hospital from July 2007 to July 2008. A 300 mg loading dose was administered prior to PCI. Then they were randomized to receive clopidogrel 75 mg (n = 55) or 150 mg (n = 46) daily for 30 days. From Day 30 to Month 6 post-operation, all of them received 75 mg/d clopidogrel and were followed up for a mean period of 6 months. RESULTS: Thirty days after PCI, the platelet inhibition of the 150 mg group was significantly higher than the 75 mg group (64.2% ± 13.3% vs 52.6% ± 14.3%, P = 0.00). The ratios of fatal or non-fatal myocardial infarction (MI) (1(1.8%) vs 3 (6.5%), P = 0.405) and target vessel revascularization (TVR) (4(7.2%) vs 6 (13.0%), P = 0.714) were significantly lower in the 150 mg group than those in the 75 mg group. So the overall incidence of MACE including death, MI and TVR was obviously lower in the 150 mg group than that in the 75 mg group (13.0% vs 20.2%, absolute risk reduction 7.3%). CONCLUSION: A high clopidogrel maintenance dose of 150 mg daily for the first month after PCI reduces the risk of long-term adverse events in patients with elective percutaneous coronary intervention.


Asunto(s)
Enfermedad de la Arteria Coronaria/tratamiento farmacológico , Inhibidores de Agregación Plaquetaria/administración & dosificación , Ticlopidina/análogos & derivados , Anciano , Clopidogrel , Enfermedad de la Arteria Coronaria/terapia , Femenino , Humanos , Masculino , Persona de Mediana Edad , Intervención Coronaria Percutánea , Inhibidores de Agregación Plaquetaria/uso terapéutico , Estudios Prospectivos , Ticlopidina/administración & dosificación , Ticlopidina/uso terapéutico , Resultado del Tratamiento
5.
Foods ; 11(21)2022 Nov 07.
Artículo en Inglés | MEDLINE | ID: mdl-36360150

RESUMEN

Exopolysaccharide (EPS) from probiotic Enterococcus hirae WEHI01 was isolated and purified by anion exchange chromatography and gel chromatography, the results of which show that the EPS consists of four fractions, namely I01-1, I01-2, I01-3, and I01-4. As the main purification components, I01-2 and I01-4 were preliminarily characterized for their structure and their immunomodulatory activity was explored. The molecular weight of I01-2 was 2.28 × 104 Da, which consists mainly of galactose, and a few other sugars including glucose, arabinose, mannose, xylose, fucose, and rhamnose, while the I01-4 was composed of galactose only and has a molecular weight of 2.59 × 104 Da. Furthermore, the results of an evaluation of immunomodulatory activity revealed that I01-2 and I01-4 could improve the viability of macrophage cells, improve phagocytosis, boost NO generation, and encourage the release of cytokines including TNF-α and IL-6 in RAW 264.7 macrophages. These results imply that I01-2 and I01-4 could improve macrophage-mediated immune responses and might be useful in the production of functional food and medications.

6.
Int J Biol Macromol ; 163: 985-995, 2020 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-32629060

RESUMEN

Two exopolysaccharides, NPS and APS, were isolated from L. plantarum WLPL09 and purified by ion-exchange chromatography. The structural analyses showed that molecular weight of NPS and APS were 72.60 kDa and 33.22 kDa, respectively. NPS was mainly composed of mannose and glucose, in molar ratio of 85.35:14.65, while APS was composed of mannose, glucose and galactose, in molar ratio of 89.69:8.65:1.66. In in vitro antitumor assays, APS displayed strong anti-proliferative effect against HepG2 hepatocellular carcinoma cells and HCT-8 colon adenocarcinoma cells in a dose-dependent manner. Morphological and flow cytometry analyses revealed that APS strongly induced apoptosis of HepG2 and HCT-8, especially for HCT-8. Furthermore, APS significantly up-regulated the mRNA level of apoptosis-related genes in cancer cells, and remarkably improved the activities of caspase-3, -8 and -9 in HepG2, caspase-3 and -8 in HCT-8. These results suggest APS might be explored as a potential, natural antitumor agent for functional food.


Asunto(s)
Antineoplásicos/química , Antineoplásicos/farmacología , Lactobacillus plantarum/química , Leche Humana/química , Polisacáridos Bacterianos/química , Polisacáridos Bacterianos/farmacología , Antineoplásicos/aislamiento & purificación , Proteínas Reguladoras de la Apoptosis/genética , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Células Hep G2 , Humanos , Peso Molecular , Monosacáridos , Polisacáridos Bacterianos/aislamiento & purificación , Reacción en Cadena en Tiempo Real de la Polimerasa , Espectroscopía Infrarroja por Transformada de Fourier
7.
Zhonghua Xin Xue Guan Bing Za Zhi ; 37(8): 725-8, 2009 Aug.
Artículo en Zh | MEDLINE | ID: mdl-20021927

RESUMEN

OBJECTIVE: To observe the effect of glycoprotein receptor blockade tirofiban in acute anterior myocardial infarction patients without ST segment resolution after primary percutaneous coronary intervention (PCI). METHODS: From April 2006 to April 2008, 157 acute anterior myocardial infarction patients without ST segment resolution after PCI were randomly allocated to tirofiban (intravenous bolus 10 microg/kg followed by intravenous infusion of 0.15 microgxkg(-1)xmin(-1) for 48 h, n = 80) or equal volume saline (control group, n = 77). Baseline characteristics, PCI features and clinical outcomes during hospitalization, left ventricular ejection fractions (LVEF) and major adverse cardiac events (MACE, including death, re-infarction and target vessel revascularization) at 30 and 180 days after discharge were compared between the two groups. RESULTS: The baseline clinical characteristics were comparable between the two groups. Compared to control group, the MACE rates and re-infarction rates at 30 days (6.3% vs.18.2%, P < 0.05; 1.3% vs.9.1%, P < 0.05, respectively) and 180 days (10.0% vs.23.4%, P < 0.05; 2.5% vs.10.4%, P < 0.05, respectively) were significantly reduced in tirofiban group. LVEF value was significantly higher in tirofiban group at 30 days and 180 days compared with those in control group [(51 +/- 6)% vs. (46 +/- 8)%, P < 0.05; (57 +/- 7)% vs. (50 +/- 9)%, P < 0.05]. Hemorrhagic complications were similar between the two groups. CONCLUSION: Use of tirofiban for acute anterior myocardial infarction patients without ST segment resolution after PCI is safe and can significantly improve 30 and 180 days clinical outcomes after discharge.


Asunto(s)
Infarto de la Pared Anterior del Miocardio/diagnóstico , Infarto de la Pared Anterior del Miocardio/tratamiento farmacológico , Inhibidores de Agregación Plaquetaria/uso terapéutico , Tirosina/análogos & derivados , Anciano , Angioplastia Coronaria con Balón , Infarto de la Pared Anterior del Miocardio/terapia , Electrocardiografía , Femenino , Humanos , Masculino , Persona de Mediana Edad , Pronóstico , Tirofibán , Resultado del Tratamiento , Tirosina/uso terapéutico
8.
Int J Biol Macromol ; 128: 710-717, 2019 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-30594615

RESUMEN

In this study exopolysaccharide (EPS) of Enterococcus faecium WEFA23 from healthy infant's feces was yielded as high as 130 mg/L by fermentation. By purification the EPS was further fractioned into A23-1, A23-2, A23-3 and A23-4 on HiTrap Q HP and Superdex G-200 column. As the major purified fractions, A23-2 and A23-4 were analyzed for the preliminary structures and investigated for the biological properties in vitro. The molecular weight of A23-2 and A23-4 was 2.50 × 104 Da and 3.23 × 104 Da, respectively. A23-2 was composed of mannose, glucose and galactose with a ratio of 1.38:1.00:1.42, while A23-4 consisted of only mannose. The antioxidant ability of A23-2 was significantly higher than that of A23-4, as proved by scavenging test of DPPH radical, hydroxyl radical and superoxide radical. Both A23-2 and A23-4 exhibited strong inhibition against the adhesion of L. monocytogenes CMCC54007 on HT-29 cells no matter for the styles of competition, displacement and exclusion. Notably, for exclusion they had a maximal inhibition rate of 89.85 ±â€¯0.46% and 90.81 ±â€¯2.29%, respectively, at 200 µg/mL.


Asunto(s)
Enterococcus faecium/química , Polisacáridos Bacterianos/química , Polisacáridos Bacterianos/farmacología , Adhesión Bacteriana/efectos de los fármacos , Compuestos de Bifenilo/química , Heces/microbiología , Células HT29 , Humanos , Lactante , Listeria monocytogenes/efectos de los fármacos , Listeria monocytogenes/fisiología , Peso Molecular , Monosacáridos/análisis , Picratos/química
9.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 34(2): 97-104, 2018 Feb.
Artículo en Zh | MEDLINE | ID: mdl-29673450

RESUMEN

Objective To establish a multiple-indirect immunofluorescence staining that can be used to detect several proteins in the same frozen section of hepatocellular carcinoma (HCC) tissues. Methods Laser scanning confocal microscopy (LSCM) was applied to scan the first group of antibodies against four proteins stained on the HCC tissues by the indirect immunofluorescence technique. The first group of antibodies and residual dye were removed by homologous species IgG Fab antibody blocking combined with chemical strip reagents (2-mercaptoethanol/dodecyl benzene sulfonic acid sodium/guanidine hydrochloride/sodium borohydride, ß-ME/SDS/GnHCl/NaBH4). The second group of antibodies against four proteins were stained in the same section and imaged with LSCM again. Results The antibodies against eight proteins were co-stained and observed in the same section by LSCM. The antibodies of IgG Fab could close non-specific dying. After the first round of residual antibody was cleared by strip reagents, the second round of antibodies was successfully stained in the same section. Conclusion The experiment established the multiple-indirect immunofluorescence techniques for detecting multiple markers in the same tumor tissues as expected.


Asunto(s)
Biomarcadores de Tumor/química , Carcinoma Hepatocelular/química , Técnica del Anticuerpo Fluorescente Indirecta/métodos , Neoplasias Hepáticas/química , Carcinoma Hepatocelular/patología , Secciones por Congelación , Humanos , Neoplasias Hepáticas/patología , Microscopía Confocal
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