RESUMEN
AIMS: To develop an efficient and facile expression system supply of high purity and stable activity of rFip-fve for oral administration, medicinal study and applications. METHODS AND RESULTS: A recombinant virus that contained the chimera gene, encoding a bombyxin signal peptide sequence fused to a Fip-fve-6His sequence, was constructed. The rFip-fve was purified from the supernatant of the infected Sf21 cells using a nickel-chelated affinity column, and was verified by Western blot and MALDI-MS (matrix-assisted laser desorption ionization mass spectrometry) analyses. Results showed that a glycosylated mature rFip-fve was produced and secreted into the infected cell supernatant. The immunomodulatory activity of rFip-fve was evaluated by measuring the amount of interleukin-2 released from murine splenocytes. CONCLUSIONS: A reliable scheme to express and purify active rFip-fve in a baculovirus/insect cell system for medicinal applications and genetic study is a feasible means of solving potential problems related to the production and activity of rFip-fve protein. SIGNIFICANCE AND IMPACT OF THE STUDY: The rFip-fve expressed in insect cells was processed and modified in a manner more similar to that of its native counterpart than that in bacterial cells. Therefore, the potential applications of rFip-fve that is generated in Sf21 cells can be more effectively evaluated that produced in Escherichia coli.
Asunto(s)
Baculoviridae/fisiología , Proteínas Fúngicas/aislamiento & purificación , Factores Inmunológicos/aislamiento & purificación , Microbiología Industrial , Lectinas/aislamiento & purificación , Animales , Reactores Biológicos , Línea Celular , Células Cultivadas , Escherichia coli , Proteínas Fúngicas/farmacología , Factores Inmunológicos/farmacología , Interleucina-2/biosíntesis , Lectinas/farmacología , Ratones , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/farmacología , Bazo/efectos de los fármacos , Bazo/inmunología , Spodoptera/virologíaRESUMEN
Mastoparan B, a cationic toxin, is the major peptide component in the venom of Vespa basalis. Molecular cloning of its cDNA fragment revealed that this toxin was initially synthesized as a precursor polypeptide, containing an N-terminal signal sequence, a prosequence, the mature toxin, and an appendix glycine at C-terminus. Sequence alignment between precursors of mastoparan B and melittin from honeybee venom showed a significant conservation in prosequence. Alternate positions existing in both prosequences were either proline or alanine known as the potential cleaving sites for dipeptidyl peptidase IV. Subsequently, a putative dipeptidyl peptidase IV cDNA fragment was cloned from Vespa basalis venom gland. The prosequence may possibly be removed via sequential liberation of dipeptides during the processing of mastoparan B.