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Microsc Res Tech ; 75(2): 133-7, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-21761494

RESUMEN

Osteoporosis is a global public health that affects postmenopausal women due to the deficiency of estrogen, a hormone that plays an important role in the microarchitecture of bone tissue. Osteoporosis predisposes to pathological bone fracture that can be repaired by conventional methods. However, depending on the severity and quantity of bone loss, the use of autogenous grafts or biomaterials such as hydroxyapatite might be necessary. The latter has received increasing attention in the medical field because of its good biological properties such as osteoconductivity and biocompatibility with bone tissue. The objective of this study was to evaluate using histologic and radiographic analyses, the osteogenic capacity of hydroxyapatite implanted into the femur of rats with ovariectomy-induced osteoporosis. Eighteen rats were divided into three groups with six animals in each: group nonovariectomized, bilaterally ovariectomized not receiving estrogen replacement therapy, and bilaterally ovariectomized submitted to estrogen replacement therapy. Defects were created experimentally in the distal epiphysis of the femur with a surgical drill and filled with porous hydroxyapatite granules. The animals were sacrificed 8 weeks after surgery. The volume of newly formed bone in the implant area was quantified by morphometrical methods. The results were analyzed by ANOVA followed by the Tukey test (P < 0.05). The hydroxyapatite granules showed good radiopacity. Histological analysis revealed less quantity of newly formed bone in the ovariectomized group not submitted to hormone replacement therapy. In conclusion, bone neoformation can be expected even in bones compromised by estrogen deficiency, but the quantity and velocity of bone formation are lower.


Asunto(s)
Regeneración Ósea , Durapatita/administración & dosificación , Terapia de Reemplazo de Estrógeno , Fémur/efectos de los fármacos , Osteoporosis/tratamiento farmacológico , Análisis de Varianza , Animales , Durapatita/farmacología , Epífisis/diagnóstico por imagen , Epífisis/efectos de los fármacos , Epífisis/lesiones , Epífisis/patología , Estradiol/farmacología , Femenino , Fémur/diagnóstico por imagen , Fémur/lesiones , Fémur/patología , Osteogénesis/efectos de los fármacos , Osteoporosis/patología , Ovariectomía , Radiografía , Ratas
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