Levels, Patterns, and Biomagnification Potential of Perfluoroalkyl Substances in a Terrestrial Food Chain in a Nordic Skiing Area.

Perfluoroalkyl substances (PFASs) are used in a wide range of consumer products, including ski products, such as ski waxes. However, there is limited knowledge on the release of PFASs from such products into the environment and the resultant uptake in biota and transport in food webs. We investigated levels, patterns, and biomagnification of PFASs in soil, earthworms (Eisenia fetida), and Bank voles (Myodes glareolus) from a skiing area in Trondheim, Norway. In general, there was higher PFAS levels in the skiing area compared to the reference area with no skiing activities. The skiing area was dominated by long-chained perfluorocarboxylic acids (PFCAs, ≥70%), while the reference area was dominated by short-chained PFCAs (>60%). The soil PFAS pattern in the skiing area was comparable to analyzed ski waxes, indicating that ski products are important sources of PFASs in the skiing area. A biomagnification factor (BMF) > 1 was detected for Bank volewhole/earthwormwhole for perfluorooctansulfonate in the skiing area. All other PFASs showed a BMF < 1. However, it should be noted that these organisms represent the base of the terrestrial food web, and PFASs originating from ski wax may result to higher exposure in organisms at the top of the food chain.

Effects of complex organohalogen contaminant mixtures on thyroid homeostasis in hooded seal (Cystophora cristata) mother-pup pairs.

Many lipid-soluble and phenolic compounds present in the complex mixture of orgaohalogen contaminants (OHCs) that arctic wildlife is exposed to have the ability to interfere with the thyroid hormone (TH) system. The aim of this study was to identify compounds that might interfere with thyroid homeostasis in 14 nursing hooded seal (Cystophora cristata) mothers and their pups (1-4d old) sampled in the West Ice in March 2008. Multivariate modelling was used to assess the potential effects of measured plasma levels of OHCs on circulating TH levels of the measured free (F) and total (T) levels of triidothyrine (T3) and thyroxine (T4). Biological factors were important in all models (e.g. age and sex). In both mothers and pups, TT3:FT3 ratios were associated with α- and ß-hexachlorocyclohexane (HCH), ortho-PCBs, chlordanes and DDTs. The similarities between the modelled TT3:FT3 responses to OHC levels in hooded seal mothers and pups most probably reflects similar exposure patterns, but could also indicate interconnected TH responses. There were some differences in the modelled TH responses of mothers and pups. Most importantly, the negative relationships between many OH-PCBs (particularly 3'-OH-CB138) and TT3:FT3 ratio and the positive relationships between TT4:FT4 ratios and polybrominated diphenyl ether [PBDE]-99, -100 and 4-OH-CB107 in pups, which was not found in mothers. Although statistical associations are not evidence per se of biological cause-effect relationships, the results suggest that thyroid homeostasis is affected in hooded seals, and that the inclusion of the fullest possible OHC mixture is important when assessing TH related effects in wildlife.

Levels and patterns of hydroxylated polychlorinated biphenyls (OH-PCBs) and their associations with thyroid hormones in hooded seal (Cystophora cristata) mother-pup pairs.

Blood (plasma/serum) samples from 14 adult female and their pups (1-4 days old) captured in the West Ice, east of Greenland were analysed for concentrations of total and free thyroxine and triiodothyronine (TT4, FT4, TT3, FT3), and hydroxylated polychlorinated biphenyls (OH-PCBs). The levels of all thyroid hormones (THs) were significantly higher in pups than in mothers. Sum OH-PCB levels (ΣOH-PCBs: 4-OH-CB107, 3'-OH-CB138, 4-OH-CB146, 4'-OH-CB172, 4-OH-CB187) were significantly higher in mothers (3.98 ± 1.55 pmol/g; 1.40 ± 0.54 ng/g wet weight) as compared to pups (1.95 ± 0.78 pmol/g; 0.68 ± 0.28 ng/g wet weight). Plasma levels of TT4 and FT4 in mothers increased as a function of pup age, as did levels of individual OH-PCBs in both mothers and pups. The pattern of OH-PCBs in the pups was similar to their mothers. We suggest that OH-PCBs found in pups are transferred from their mothers during gestation and that the transfer also continues after parturition via milk. Principal component analysis (PCA) showed that in pups, 4-OH-CB107 and 3'-OH-CB138 were negatively associated with FT4:FT3 and TT3:FT3 ratios, respectively. These relationships were confirmed by partial correlation analysis correcting for pup age. PCA suggested that 4'-OH-CB172 and 4-OH-CB187 were negatively associated with TT3 in mothers. However, this was not confirmed by correlation tests. Although statistical relationships should be interpreted with caution, the study indicates that young developing seals are more sensitive compared to adults with respect to TH-related effects of OH-PCBs.