Apidima Cave fossils provide earliest evidence of Homo sapiens in Eurasia.

Two fossilized human crania (Apidima 1 and Apidima 2) from Apidima Cave, southern Greece, were discovered in the late 1970s but have remained enigmatic owing to their incomplete nature, taphonomic distortion and lack of archaeological context and chronology. Here we virtually reconstruct both crania, provide detailed comparative descriptions and analyses, and date them using U-series radiometric methods. Apidima 2 dates to more than 170 thousand years ago and has a Neanderthal-like morphological pattern. By contrast, Apidima 1 dates to more than 210 thousand years ago and presents a mixture of modern human and primitive features. These results suggest that two late Middle Pleistocene human groups were present at this site-an early Homo sapiens population, followed by a Neanderthal population. Our findings support multiple dispersals of early modern humans out of Africa, and highlight the complex demographic processes that characterized Pleistocene human evolution and modern human presence in southeast Europe.

Humans thrived in South Africa through the Toba eruption about 74,000 years ago.

Approximately 74 thousand years ago (ka), the Toba caldera erupted in Sumatra. Since the magnitude of this eruption was first established, its effects on climate, environment and humans have been debated. Here we describe the discovery of microscopic glass shards characteristic of the Youngest Toba Tuff-ashfall from the Toba eruption-in two archaeological sites on the south coast of South Africa, a region in which there is evidence for early human behavioural complexity. An independently derived dating model supports a date of approximately 74 ka for the sediments containing the Youngest Toba Tuff glass shards. By defining the input of shards at both sites, which are located nine kilometres apart, we are able to establish a close temporal correlation between them. Our high-resolution excavation and sampling technique enable exact comparisons between the input of Youngest Toba Tuff glass shards and the evidence for human occupation. Humans in this region thrived through the Toba event and the ensuing full glacial conditions, perhaps as a combined result of the uniquely rich resource base of the region and fully evolved modern human adaptation.

An early and enduring advanced technology originating 71,000 years ago in South Africa.

There is consensus that the modern human lineage appeared in Africa before 100,000 years ago. But there is debate as to when cultural and cognitive characteristics typical of modern humans first appeared, and the role that these had in the expansion of modern humans out of Africa. Scientists rely on symbolically specific proxies, such as artistic expression, to document the origins of complex cognition. Advanced technologies with elaborate chains of production are also proxies, as these often demand high-fidelity transmission and thus language. Some argue that advanced technologies in Africa appear and disappear and thus do not indicate complex cognition exclusive to early modern humans in Africa. The origins of composite tools and advanced projectile weapons figure prominently in modern human evolution research, and the latter have been argued to have been in the exclusive possession of modern humans. Here we describe a previously unrecognized advanced stone tool technology from Pinnacle Point Site 5-6 on the south coast of South Africa, originating approximately 71,000 years ago. This technology is dominated by the production of small bladelets (microliths) primarily from heat-treated stone. There is agreement that microlithic technology was used to create composite tool components as part of advanced projectile weapons. Microliths were common worldwide by the mid-Holocene epoch, but have a patchy pattern of first appearance that is rarely earlier than 40,000 years ago, and were thought to appear briefly between 65,000 and 60,000 years ago in South Africa and then disappear. Our research extends this record to ~71,000 years, shows that microlithic technology originated early in South Africa, evolved over a vast time span (~11,000 years), and was typically coupled to complex heat treatment that persisted for nearly 100,000 years. Advanced technologies in Africa were early and enduring; a small sample of excavated sites in Africa is the best explanation for any perceived 'flickering' pattern.

Interpreting human behavior from depositional rates and combustion features through the study of sedimentary microfacies at site Pinnacle Point 5-6, South Africa.

Using fine and coarse resolution geoarchaeological studies at the Middle Stone Age site of PP5-6 at Pinnacle Point, Mossel Bay, South Africa, we discovered different patterns of anthropogenic input and changes in behavior through time. Through the microfacies approach, we documented the various geogenic and anthropogenic processes that formed the deposits of the site. By deciphering large scale rate differences in the production of these microfacies we estimated anthropogenic input rates and therefore gained understanding of occupational duration and intensity. The PP5-6 sediments document occupations characterized by small groups and short visits during Marine Isotope Stage (MIS) 5. This part of the sequence is characterized by numerous single (and mostly intact) hearth structures in a roofspall-rich matrix. During this time the sea was very close to the site and the people were focused on exploiting the rocky shores. With the advent of the glacial conditions of MIS4, the occupation of the site became much more intense. The occurrence of thick palimpsests of burnt remains, sometimes disturbed by small-scale sedimentary gravity processes, supports this conclusion. As sea level dropped and the coastline retreated, the geogenic input shifted to predominately aeolian sediments, implying an exposed shelf probably associated with a rich but more distant coastal environment. The occupants of PP5-6 turned their preference to silcrete as a raw material and they began to make microlithic stone tools. Since sites dating to MIS4 are abundant in the Cape, we suggest that populations during MIS4 responded to glacial conditions with either demographic stability or growth as well as technological change.

Volcanic ash layers illuminate the resilience of Neanderthals and early modern humans to natural hazards.

Marked changes in human dispersal and development during the Middle to Upper Paleolithic transition have been attributed to massive volcanic eruption and/or severe climatic deterioration. We test this concept using records of volcanic ash layers of the Campanian Ignimbrite eruption dated to ca. 40,000 y ago (40 ka B.P.). The distribution of the Campanian Ignimbrite has been enhanced by the discovery of cryptotephra deposits (volcanic ash layers that are not visible to the naked eye) in archaeological cave sequences. They enable us to synchronize archaeological and paleoclimatic records through the period of transition from Neanderthal to the earliest anatomically modern human populations in Europe. Our results confirm that the combined effects of a major volcanic eruption and severe climatic cooling failed to have lasting impacts on Neanderthals or early modern humans in Europe. We infer that modern humans proved a greater competitive threat to indigenous populations than natural disasters.

On the chronology of the Uluzzian.

The Uluzzian, one of Europe's 'transitional' technocomplexes, has gained particular significance over the past three years when the only human remains associated with it were attributed to modern humans, instead of Neanderthals as previously thought. The position of the Uluzzian at stratified sequences, always overlying late Mousterian layers and underlying early Upper Palaeolithic ones, highlights its significance in understanding the passage from the Middle to Upper Palaeolithic, as well as the replacement of Neanderthals by modern humans in southeastern Mediterranean Europe. Despite several studies investigating aspects of its lithic techno-typology, taxonomy and material culture, the Uluzzian chronology has remained extremely poorly-known, based on a handful of dubious chronometric determinations. Here we aim to elucidate the chronological aspect of the technocomplex by presenting an integrated synthesis of new radiocarbon results and a Bayesian statistical approach from four stratified Uluzzian cave sequences in Italy and Greece (Cavallo, Fumane, Castelcivita and Klissoura 1). In addition to building a reliable chronological framework for the Uluzzian, we examine its appearance, tempo-spatial spread and correlation to previous and later Palaeolithic assemblages (Mousterian, Protoaurignacian) at the relevant regions. We conclude that the Uluzzian arrived in Italy and Greece shortly before 45,000 years ago and its final stages are placed at ∼39,500 years ago, its end synchronous (if not slightly earlier) with the Campanian Ignimbrite eruption.

Life-history of Palaeoloxodon antiquus reveals Middle Pleistocene glacial refugium in the Megalopolis basin, Greece.

The Balkans are considered a major glacial refugium where flora and fauna survived glacial periods and repopulated the rest of Europe during interglacials. While it is also thought to have harboured Pleistocene human populations, evidence linking human activity, paleoenvironmental indicators and a secure temporal placement to glacial periods is scant. Here, we present the first intra-tooth multi-isotope analysis for the European straight-tusked elephant Palaeoloxodon antiquus, on an adult male individual excavated in association with lithic artefacts at the MIS 12 site Marathousa 1 (Megalopolis basin, Greece). The studied find also exhibits anthropogenic modifications, providing direct evidence of hominin presence. We employed strontium, carbon and oxygen isotope analysis on enamel bioapatite to investigate its foraging and mobility behaviour, using a sequential sampling strategy along the tooth growth axis of the third upper molar, to assess ecological changes during the last decade of life. We found a geographically restricted range, in a C3-dominated open woodland environment, and relatively stable conditions over the examined timeframe. Our results show that, despite the severity of the MIS 12 glacial, the Megalopolis basin sustained a mesic habitat, sufficient plant cover and limited seasonal fluctuations in resource availability, pointing to its role as a glacial refugium for both fauna and hominins.

Early human use of marine resources and pigment in South Africa during the Middle Pleistocene.

Genetic and anatomical evidence suggests that Homo sapiens arose in Africa between 200 and 100 thousand years (kyr) ago, and recent evidence indicates symbolic behaviour may have appeared approximately 135-75 kyr ago. From 195-130 kyr ago, the world was in a fluctuating but predominantly glacial stage (marine isotope stage MIS6); much of Africa was cooler and drier, and dated archaeological sites are rare. Here we show that by approximately 164 kyr ago (+/-12 kyr) at Pinnacle Point (on the south coast of South Africa) humans expanded their diet to include marine resources, perhaps as a response to these harsh environmental conditions. The earliest previous evidence for human use of marine resources and coastal habitats was dated to approximately 125 kyr ago. Coincident with this diet and habitat expansion is an early use and modification of pigment, probably for symbolic behaviour, as well as the production of bladelet stone tool technology, previously dated to post-70 kyr ago. Shellfish may have been crucial to the survival of these early humans as they expanded their home ranges to include coastlines and followed the shifting position of the coast when sea level fluctuated over the length of MIS6.

Ancient DNA reveals admixture history and endogamy in the prehistoric Aegean.

The Neolithic and Bronze Ages were highly transformative periods for the genetic history of Europe but for the Aegean-a region fundamental to Europe's prehistory-the biological dimensions of cultural transitions have been elucidated only to a limited extent so far. We have analysed newly generated genome-wide data from 102 ancient individuals from Crete, the Greek mainland and the Aegean Islands, spanning from the Neolithic to the Iron Age. We found that the early farmers from Crete shared the same ancestry as other contemporaneous Neolithic Aegeans. In contrast, the end of the Neolithic period and the following Early Bronze Age were marked by 'eastern' gene flow, which was predominantly of Anatolian origin in Crete. Confirming previous findings for additional Central/Eastern European ancestry in the Greek mainland by the Middle Bronze Age, we additionally show that such genetic signatures appeared in Crete gradually from the seventeenth to twelfth centuries BC, a period when the influence of the mainland over the island intensified. Biological and cultural connectedness within the Aegean is also supported by the finding of consanguineous endogamy practiced at high frequencies, unprecedented in the global ancient DNA record. Our results highlight the potential of archaeogenomic approaches in the Aegean for unravelling the interplay of genetic admixture, marital and other cultural practices.

Site formation processes at Pinnacle Point Cave 13B (Mossel Bay, Western Cape Province, South Africa): resolving stratigraphic and depositional complexities with micromorphology.

Site PP13B is a cave located on the steep cliffs of Pinnacle Point near Mossel Bay in Western Cape Province, South Africa. The depositional sequence of the cave, predating Marine Isotopic Stage 11 (MIS 11) and continuing to present, is in the form of isolated sediment exposures with different depositional facies and vertical and lateral variations. Micromorphological analysis demonstrated that a suite of natural sedimentation processes operated during the development of the sequence ranging from water action to aeolian activity, and from speleothem formations to plant colonization and root encrustation. At the same time, anthropogenic sediments that are mainly in the form of burnt remains from combustion features (e.g., wood ash, charcoal, and burnt bone) were accumulating. Several erosional episodes have resulted in a complicated stratigraphy, as discerned from different depositional and post-depositional features. The cave is associated with a fluctuating coastal environment, frequent changes in sea level and climate controlled patterns of sedimentation, and the presence or absence of humans.

The stratigraphy of the Middle Stone Age sediments at Pinnacle Point Cave 13B (Mossel Bay, Western Cape Province, South Africa).

Pinnacle Point Cave 13B (PP13B) has provided the earliest archaeological evidence for the exploitation of marine shellfish, along with very early evidence for use and modification of pigments and the production of bladelets, all dated to approximately 164 ka (Marean et al., 2007). This makes PP13B a key site in studies of the origins of modern humans, one of a handful of sites in Africa dating to Marine Isotope Stage 6 (MIS 6), and the only site on the coast of South Africa with human occupation confidently dated to MIS 6. Along with this MIS 6 occupation there are rich archaeological sediments dated to MIS 5, and together these sediments are differentially preserved in three different areas of the cave. The sediments represent a complex palimpsest of geogenic, biogenic, and anthropogenic input and alteration that are described and interpreted through the use of a variety of macrostratigraphic, micromorphologic, and geochemical techniques. Three independent dating techniques allow us to constrain the age range of these sediments and together provide the stratigraphic context for the analyses of the material that follow in this special issue.

Earliest occupation of the Central Aegean (Naxos), Greece: Implications for hominin and Homo sapiens' behavior and dispersals.

We present evidence of Middle Pleistocene activity in the central Aegean Basin at the chert extraction and reduction complex of Stelida (Naxos, Greece). Luminescence dating places ~9000 artifacts in a stratigraphic sequence from ~13 to 200 thousand years ago (ka ago). These artifacts include Mousterian products, which arguably provide first evidence for Neanderthals in the region. This dated material attests to a much earlier history of regional exploration than previously believed, opening the possibility of alternative routes into Southeast Europe from Anatolia (and Africa) for (i) hominins, potentially during sea level lowstands (e.g., Marine Isotope Stage 8) permitting terrestrial crossings across the Aegean, and (ii) Homo sapiens of the Early Upper Paleolithic (Aurignacian), conceivably by sea.

Phytoliths as an indicator of early modern humans plant gathering strategies, fire fuel and site occupation intensity during the Middle Stone Age at Pinnacle Point 5-6 (south coast, South Africa).

The study of plant remains in archaeological sites, along with a better understanding of the use of plants by prehistoric populations, can help us shed light on changes in survival strategies of hunter-gatherers and consequent impacts on modern human cognition, social organization, and technology. The archaeological locality of Pinnacle Point (Mossel Bay, South Africa) includes a series of coastal caves, rock-shelters, and open-air sites with human occupations spanning the Acheulian through Middle Stone Age (MSA) and Later Stone Age (LSA). These sites have provided some of the earliest evidence for complex human behaviour and technology during the MSA. We used phytoliths-amorphous silica particles that are deposited in cells of plants-as a proxy for the reconstruction of past human plant foraging strategies on the south coast of South Africa during the Middle and Late Pleistocene, emphasizing the use and control of fire as well as other possible plant uses. We analysed sediment samples from the different occupation periods at the rock shelter Pinnacle Point 5-6 North (PP5-6N). We also present an overview of the taphonomic processes affecting phytolith preservation in this site that will be critical to conduct a more reliable interpretation of the original plant use in the rock shelter. Our study reports the first evidence of the intentional gathering and introduction into living areas of plants from the Restionaceae family by MSA hunter-gatherers inhabiting the south coast of South Africa. We suggest that humans inhabiting Pinnacle Point during short-term occupation events during Marine Isotope Stage (MIS) 5 built fast fires using mainly grasses with some wood from trees and/or shrubs for specific purposes, perhaps for shellfish cooking. With the onset of MIS 4 we observed a change in the plant gathering strategies towards the intentional and intensive exploitation of dry wood to improve, we hypothesise, combustion for heating silcrete. This human behaviour is associated with changes in stone tool technology, site occupation intensity and climate change.

Of cattle and feasts: Multi-isotope investigation of animal husbandry and communal feasting at Neolithic Makriyalos, northern Greece.

The aim of this study is to investigate livestock husbandry and its relationship to the mobilization of domestic animals for slaughter at large communal feasting events, in Late Neolithic Makriyalos, northern Greece. A multi-isotope approach is built that integrates analysis of: δ13C and δ15N values of human and animal bone collagen for understanding long-term dietary behavior,Incremental δ13C and δ18O values of domestic animal tooth enamel carbonate for assessing seasonal patterns in grazing habits and mobility, and87Sr/86Sr ratios of cattle tooth enamel for examining the possibility that some of the animals consumed at the site were born outside the local environment. The findings indicate that cattle had isotopically more variable diets than sheep, which may reflect grazing over a wider catchment area in the local landscape. Cattle products did not make a significant contribution to the long-term dietary protein intake of the humans, which may indicate that they were primarily consumed during episodic feasting events. There is no indication that pasturing of livestock was pre-determined by their eventual context of slaughter (i.e. large-scale feasting vs. more routine consumption events). Two non-local cattle identified among those deposited in a feasting context may have been brought to the site as contributions to these feasts. The evidence presented provides a more detailed insight into local land use and into the role of livestock and feasting in forging social relationships within the regional human population.

Evidence for habitual use of fire at the end of the Lower Paleolithic: site-formation processes at Qesem Cave, Israel.

The Amudian (late Lower Paleolithic) site of Qesem Cave in Israel represents one of the earliest examples of habitual use of fire by middle Pleistocene hominids. The Paleolithic layers in this cave were studied using a suite of mineralogical and chemical techniques and a contextual sedimentological analysis (i.e., micromorphology). We show that the lower ca. 3m of the stratigraphic sequence are dominated by clastic sediments deposited within a closed karstic environment. The deposits were formed by small-scale, concentrated mud slurries (infiltrated terra rosa soil) and debris flows. A few intervening lenses of mostly in situ burnt remains were also identified. The main part of the upper ca. 4.5 m consists of anthropogenic sediment with only moderate amounts of clastic geogenic inputs. The deposits are strongly cemented with calcite that precipitated from dripping water. The anthropogenic component is characterized by completely combusted, mostly reworked wood ash with only rare remnants of charred material. Micromorphological and isotopic evidence indicates recrystallization of the wood ash. Large quantities of burnt bone, defined by a combination of microscopic and macroscopic criteria, and moderately heated soil lumps are closely associated with the wood-ash remains. The frequent presence of microscopic calcified rootlets indicates that the upper sequence formed in the vicinity of the former cave entrance. Burnt remains in the sediments are associated with systematic blade production and faunas that are dominated by the remains of fallow deer. Use-wear damage on blades and blade tools in conjunction with numerous cut marks on bones indicate an emphasis on butchering and prey-defleshing activities in the vicinity of fireplaces.

Integrated analytical techniques for the study of ancient Greek polychromy.

The materials used in the decoration of three painted astragaloi (knucklebones) from the Koroneia cave (Greece) were investigated by means of sequential application of non-destructive and destructive techniques: optical microscopy, environmental scanning electron microscopy coupled with X-ray microanalysis (ESEM-EDX), Fourier transform infrared spectroscopy (FTIR) with micro-attenuated total reflection (mu-ATR) technique, high performance liquid chromatography (HPLC) coupled with UV-fluorescence and gas chromatography-mass spectrometry (GC-MS) were used. The main results highlighted that the three astragaloi were prepared with a ground of ochre or iron clay and painted with a proteinaceous matter such as binder egg tempera. Both FTIR and GC-MS agree in the detection of lipids that can be related to egg. Organic dyestuffs identified as madder lake and shellfish purple were used together with inorganic pigments.