Microparticles manipulation and enhancement of their separation in pinched flow fractionation by insulator-based dielectrophoresis.

The separation and manipulation of microparticles in lab on a chip devices have importance in point of care diagnostic tools and analytical applications. The separation and sorting of particles from biological and clinical samples can be performed using active and passive techniques. In passive techniques, no external force is applied while in active techniques by applying external force (e.g. electrical), higher separation efficiency is obtained. In this article, passive (pinched flow fractionation) and active (insulator-based dielectrophoresis) methods were combined to increase the separation efficiency at lower voltages. First by simulation, appropriate values of geometry and applied voltages for better focusing, separation, and lower Joule heating were obtained. Separation of 1.5 and 6 µm polystyrene microparticles was experimentally obtained at optimized geometry and low total applied voltage (25 V). Also, the trajectory of 1.5 µm microparticles was controlled by adjusting the total applied voltage.

Focusing and continuous separation of microparticles by insulator-based dielectrophoresis (iDEP) in stair-shaped microchannel.

Focusing and separation of microparticles in a complex mixture have had wide applications in chemistry, biology, medicine, etc. This work presents a numerical and experimental investigation on focusing and continuous separation of microparticles in a geometrically optimized arrangement of steps in the form of a staircase using insulator-based dielectrophoresis (iDEP) mechanism. First, a detailed finite element analysis was performed on important parameters in the focusing and separation of microparticles, such as geometry of stair-shaped microchannel, total voltage, and voltage difference applied to reservoirs. The optimum parameters obtained from numerical analysis were used for experimental work. Theoretically, predicted microparticle trajectories are in good agreement with experimentally observed ones. Experimental and numerical results show that the performance of focusing of microparticles enhances with growth of the total voltage (in a constant voltage difference) and decreases with voltage difference. The fabricated iDEP microchip enhances the performance of focusing and separation of microparticles due to its stair-shaped microchannel and therefore operates at low DC total applied voltages of 90-110 V.