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1.
Antimicrob Agents Chemother ; : e0034124, 2024 May 14.
Artículo en Inglés | MEDLINE | ID: mdl-38742905

RESUMEN

Cell culture-based screening of a chemical library identified diphenoxylate as an antiviral agent against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The observed 50% effective concentrations ranged between 1.4 and 4.9 µM against the original wild-type strain and its variants. Time-of-addition experiments indicated that diphenoxylate is an entry blocker targeting a host factor involved in viral infection. Fluorescence microscopic analysis visualized that diphenoxylate prevented SARS-CoV-2 particles from penetrating the cell membrane and also impaired endo-lysosomal acidification. Diphenoxylate exhibited a synergistic inhibitory effect on SARS-CoV-2 infection in human lung epithelial Calu-3 cells when combined with a transmembrane serine protease 2 (TMPRSS2) inhibitor, nafamostat. This synergy suggested that efficient antiviral activity is achieved by blocking both TMPRSS2-mediated early and endosome-mediated late SARS-CoV-2 entry pathways. The antiviral efficacy of diphenoxylate against SARS-CoV-2 was reproducible in a human tonsil organoids system. In a transgenic mouse model expressing the obligate SARS-CoV-2 receptor, human angiotensin-converting enzyme 2, intranasal administration of diphenoxylate (10 mg/kg/day) significantly reduced the viral RNA copy number in the lungs by 70% on day 3. This study underscores that diphenoxylate represents a promising core scaffold, warranting further exploration for chemical modifications aimed at developing a new class of clinically effective antiviral drugs against SARS-CoV-2.

2.
Analyst ; 149(1): 254, 2023 Dec 18.
Artículo en Inglés | MEDLINE | ID: mdl-38047466

RESUMEN

Correction for 'Quantitative assessment of cardiomyocyte mechanobiology through high-throughput cantilever-based functional well plate systems' by Jongyun Kim et al., Analyst, 2023, 148, 5133-5143, https://doi.org/10.1039/D3AN01286G.

3.
Analyst ; 148(20): 5133-5143, 2023 Oct 05.
Artículo en Inglés | MEDLINE | ID: mdl-37695027

RESUMEN

Proper regulation of the in vitro cell culture environment is essential for disease modelling and drug toxicity screening. The main limitation of well plates used for cell culture is that they cannot accurately maintain energy sources and compounds needed during cell growth. Herein, to understand the importance of perfusion in cardiomyocyte culture, changes in contractile force and heart rate during cardiomyocyte growth are systematically investigated, and the results are compared with those of a perfusion-free system. The proposed perfusion system consists of a Peltier refrigerator, a peristaltic pump, and a functional well plate. A functional well plate with 12 wells is made through injection moulding, with two tubes integrated in the cover for each well to continuously circulate the culture medium. The contractile force of cardiomyocytes growing on the cantilever surface is analysed through changes in cantilever displacement. The maturation of cardiomyocytes is evaluated through fluorescence staining and western blot; cardiomyocytes cultured in the perfusion system show greater maturity than those cultured in a manually replaced culture medium. The pH of the culture medium manually replaced at intervals of 3 days decreases to 6.8, resulting in an abnormal heartbeat, while cardiomyocytes cultured in the perfusion system maintained at pH 7.4 show improved contractility and a uniform heart rate. Two well-known ion channel blockers, verapamil and quinidine, are used to measure changes in the contractile force of cardiomyocytes from the two systems. Cardiomyocytes in the perfusion system show greater stability during drug toxicity screening, proving that the perfusion system provides a better environment for cell growth.


Asunto(s)
Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos , Miocitos Cardíacos , Humanos , Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos/metabolismo , Técnicas de Cultivo de Célula , Verapamilo/farmacología , Evaluación Preclínica de Medicamentos , Células Cultivadas
4.
Int J Phytoremediation ; 25(2): 146-160, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-35475946

RESUMEN

In this study, we used a simple and low-toxicity chemical treatment to make a carboxylate-functionalized dragon fruit peel powder (CF-DFPP) from dragon fruit peel to improve its capacity for adsorbing Rhodamine B (RhB) from an aqueous medium. Field Emission-Scanning Electron Microscopy/Energy-Dispersive X-ray (FE-SEM/EDX), point of zero charges (pHPZC), Brunauer-Emmett-Teller (BET), and Fourier Transform Infrared (FT-IR) analyses were performed to characterize the adsorbent materials. The adsorption performance and mechanism for the removal of RhB were examined. The kinetic, isotherm and thermodynamic parameters were employed to evaluate the adsorption mechanism. Compared to other models, the Langmuir isotherm and PSO kinetic models better defined the experimental data. CF-DFPP adsorbent exhibited a maximum adsorption efficiency of 228.7 mg/g at 298 K for RhB adsorption. Thermodynamic analysis revealed that the adsorption of RhB by CF-DFPP was spontaneous (ΔGo < 0) and exothermic (ΔHo < 0) nature of the process. Different eluting agents were used in desorption tests, and NaOH was revealed to have greater desorption efficiency (96.8%). Furthermore, regeneration examinations revealed that the biosorbent could effectively retain RhB, even after six adsorption/desorption cycles. These findings demonstrated that the CF-DFPP might be a novel material for removing RhB from an aqueous medium.


Asunto(s)
Frutas , Contaminantes Químicos del Agua , Polvos/análisis , Frutas/química , Adsorción , Espectroscopía Infrarroja por Transformada de Fourier , Biodegradación Ambiental , Termodinámica , Cinética , Contaminantes Químicos del Agua/química , Concentración de Iones de Hidrógeno
5.
Analyst ; 147(21): 4793-4803, 2022 Oct 24.
Artículo en Inglés | MEDLINE | ID: mdl-36189885

RESUMEN

To date, several smart stents have been proposed to continuously detect biological cues, which is essential for tracking patients' critical vital signs and therapy. However, the proposed smart stent fabrication techniques rely on conventional laser micro-cutting or 3D printing technologies. The sensors are then integrated into the stent structure using an adhesive, conductive epoxy, or laser micro-welding process. The sensor packaging method using additional fabrication processes can cause electrical noise, and there is a possibility of sensor detachment from the sent structure after implantation, which may pose a significant risk to patients. Herein, we are demonstrating for the first time a single-step fabrication method to develop a smart stent with an integrated sensor for detecting in-stent restenosis and assessing the functional dynamics of the heart. The smart stent is fabricated using a microelectromechanical system (MEMS)-based micromachining technology. The proposed smart stent can detect biological cues without additional power and wirelessly transmit the signal to the network analyzer. The cytocompatibility of the smart stent is confirmed through a cytotoxicity test by monitoring the cell growth, proliferation, and viability of the cultured cardiomyocytes. The capacitance of the smart stent exhibits an excellent linear relationship with the applied pressure. The exceptional sensitivity of the pressure sensor enabled the proposed smart stent to detect biological cues during in vivo analysis. The preliminary findings confirmed the proposed smart stent's higher level of structural integrity, durability and repeatability. Finally, the practical feasibility of the smart stent is demonstrated by monitoring diastole and systole at various beat rates using a phantom. The results of the phantom study showed a similar pattern to the human model, indicating the potential use of the proposed multifunctional smart stent for real-time applications.


Asunto(s)
Reestenosis Coronaria , Sistemas Microelectromecánicos , Humanos , Reestenosis Coronaria/etiología , Stents
6.
Analyst ; 146(23): 7160-7167, 2021 Nov 22.
Artículo en Inglés | MEDLINE | ID: mdl-34698735

RESUMEN

In this study, we developed a multi-layered functional cantilever for real-time force measurement of cardiomyocytes in cell culture media. The functional cantilever with a full-bridge circuit configuration was composed of one polydimethylsiloxane (PDMS) and two polyimide (PI) layers, forming two resistive sensors on each upper side of the two PI layers. The PI layers were chemically bonded using an oxygen plasma treatment, with a thin composite layer consisting of Cr/SiO2/PDMS. These greatly improved the force sensitivity and the long-term reliability of the integrated strain sensor operating in liquids. The nanogrooved PDMS top layer bonded on the upper PI layer was employed to further improve the growth of cardiomyocytes on the functional cantilever. The difference in resistance changes and response characteristics was confirmed by evaluating the characteristics of the multi-layered polymer cantilevers with half-bridge and full-bridge circuit configurations. We also employed the cantilever devices to measure the contraction force of cardiomyocytes for 16 days and side effects in real time in human-induced pluripotent stem cells treated with the cardiovascular drug verapamil. The sensor-integrated cantilever devices are expected to be utilized as a novel biomedical sensor for evaluating the mechanobiology of cardiomyocytes, as well as in drug screening tests.


Asunto(s)
Polímeros , Dióxido de Silicio , Humanos , Fenómenos Mecánicos , Contracción Miocárdica , Reproducibilidad de los Resultados
7.
Bioorg Chem ; 113: 105027, 2021 08.
Artículo en Inglés | MEDLINE | ID: mdl-34098398

RESUMEN

Psiguadial B (8), and its fluoro- (8a), chloro- (8b), and bromo- (8c) derivatives were synthesized using a sodium acetate-catalyzed single step coupling of three components: ß-caryophyllene (5), diformylphloroglucinol (11), and benzaldehyde (12). These compounds efficiently and dose-dependently decreased H2O2-induced cell death, a quantitative marker of cell death, in primary cultures of mouse cortical neurons. Psiguadial B also decreased neuronal death and accumulation of ROS induced by FeCl2 in cortical cultures. The in vitro effects of these compounds in lipopolysaccharide (LPS)-induced expression of nitric oxide (NO), and TNF-α and IL-6 by suppressing the NF-κB pathway in immune cells demonstrated their antioxidative and anti-inflammatory activity. The present findings warrant further research on the development of psiguadial B-based neuroprotective agents for the treatment of neurodegenerative diseases, acute brain injuries and immunological disorders.


Asunto(s)
Antiinflamatorios/química , Antioxidantes/química , Fármacos Neuroprotectores/química , Terpenos/química , Animales , Antiinflamatorios/síntesis química , Antiinflamatorios/farmacología , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Corteza Cerebral/citología , Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/metabolismo , Compuestos Ferrosos/farmacología , Halogenación , Peróxido de Hidrógeno/farmacología , Lipopolisacáridos/farmacología , Ratones , Ratones Endogámicos ICR , FN-kappa B/metabolismo , Fármacos Neuroprotectores/síntesis química , Fármacos Neuroprotectores/farmacología , Óxido Nítrico/metabolismo , Psidium/química , Psidium/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo
8.
Sensors (Basel) ; 21(7)2021 Mar 31.
Artículo en Inglés | MEDLINE | ID: mdl-33807293

RESUMEN

Fluvial remote sensing has been used to monitor diverse riverine properties through processes such as river bathymetry and visual detection of suspended sediment, algal blooms, and bed materials more efficiently than laborious and expensive in-situ measurements. Red-green-blue (RGB) optical sensors have been widely used in traditional fluvial remote sensing. However, owing to their three confined bands, they rely on visual inspection for qualitative assessments and are limited to performing quantitative and accurate monitoring. Recent advances in hyperspectral imaging in the fluvial domain have enabled hyperspectral images to be geared with more than 150 spectral bands. Thus, various riverine properties can be quantitatively characterized using sensors in low-altitude unmanned aerial vehicles (UAVs) with a high spatial resolution. Many efforts are ongoing to take full advantage of hyperspectral band information in fluvial research. Although geo-referenced hyperspectral images can be acquired for satellites and manned airplanes, few attempts have been made using UAVs. This is mainly because the synthesis of line-scanned images on top of image registration using UAVs is more difficult owing to the highly sensitive and heavy image driven by dense spatial resolution. Therefore, in this study, we propose a practical technique for achieving high spatial accuracy in UAV-based fluvial hyperspectral imaging through efficient image registration using an optical flow algorithm. Template matching algorithms are the most common image registration technique in RGB-based remote sensing; however, they require many calculations and can be error-prone depending on the user, as decisions regarding various parameters are required. Furthermore, the spatial accuracy of this technique needs to be verified, as it has not been widely applied to hyperspectral imagery. The proposed technique resulted in an average reduction of spatial errors by 91.9%, compared to the case where the image registration technique was not applied, and by 78.7% compared to template matching.

9.
Chem Rev ; 117(13): 8977-9015, 2017 Jul 12.
Artículo en Inglés | MEDLINE | ID: mdl-28060495

RESUMEN

Transition-metal-catalyzed activation of C-H and C-C bonds is a challenging area in synthetic organic chemistry. Among various methods to accomplish these processes, the approach using metal-organic cooperative catalytic systems is one of the most promising. In this protocol, organic molecules as well as transition metals act as catalysts to bring about reactions, which proceed with high efficiencies and selectivities. Various metal-organic cooperative catalytic systems developed for C-H and C-C bond activation reactions are discussed in this review. Also discussed are how each metal-organic cooperative catalyst affects the reaction mechanism and what kinds of substrates can be applied in each of the catalytic processes.

10.
Ecotoxicol Environ Saf ; 167: 385-395, 2019 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-30366272

RESUMEN

Heterogeneous Fenton-like catalyst and its industrial application are increasingly given importance for its non-selective mineralization of organic pollutants in broad pH range. Current study, utilized an aromatic hydroxamic acid derivative 2-hydroxypyridine-N-oxide (HpO), for the construction of iron-Hpo ligand catalyst supported on granular activated carbon (GAC). 8-Hydroxyquinoline and citric acid as non-hydroxamic aromatic and aliphatic Fenton-like catalysts were used for comparative evaluation of the efficiency with targeted catalyst (iron-HpO-GAC). This novel catalyst iron-HpO-GAC exhibits excellent efficiency in Acid Red 88 dye removal in the presence of hydrogen peroxide as oxidant at acidic, basic as well as at neutral conditions. Operational conditions for the catalytic oxidation including temperature, dye concentration, pH and catalyst dosage were systematically investigated and analyzed through kinetic studies. Thermodynamic analysis of the catalytic dye removal revealed that the system could oxidize pollutants faster with less activation energy requirement. Higher level of recyclability and stability of the catalyst with less iron leaching was achieved. Finally, the real time application of the catalyst was investigated through successful repeated treatment for actual industrial wastewater. The phytotoxicity assay (with respect to plant Phaseolus mungo) revealed that the degradation of Acid Red 88 and dye wastewater produced nontoxic metabolites which increases its potential application. This study emphasizes the viability of hydroxamate mediated efficient Fenton-like oxidation as a novel approach in designing economically viable pollutant removal technology.


Asunto(s)
Compuestos Azo/química , Ácidos Hidroxámicos/química , Aguas Residuales/química , Contaminantes Químicos del Agua/química , Compuestos Azo/análisis , Compuestos Azo/toxicidad , Catálisis , Carbón Orgánico/química , Compuestos Férricos/química , Peróxido de Hidrógeno/química , Cinética , Oxidación-Reducción , Textiles , Vigna/efectos de los fármacos , Aguas Residuales/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad
11.
Bioorg Med Chem ; 26(15): 4382-4389, 2018 08 15.
Artículo en Inglés | MEDLINE | ID: mdl-30054191

RESUMEN

In this study, we designed and synthesized several novel "Y"-shaped biaryl PPARδ agonists. Structure-activity relationship (SAR) studies demonstrated that compound 3a was the most active agonist with an EC50 of 2.6 nM. We also synthesized and evaluated enantiospecific R and S isomers of compound 3a to confirm that R isomer (EC50 = 0.7 nM) shows much more potent activity than S isomer (EC50 = 6.1 nM). Molecular docking studies between the PPAR ligand binding domain and enantiospecific R and S isomers of compound 3a were performed. In vitro absorption, distribution, metabolism, excretion, and toxicity (ADMET) and in vivo PK profiles show that compound 3a possesses superior drug-like properties including good bioavailability. Our overall results clearly demonstrate that this orally administrable PPARδ agonist 3a is a viable drug candidate for the treatment of various PPARδ-related disorders.


Asunto(s)
Acetatos/química , Compuestos de Bifenilo/síntesis química , PPAR delta/agonistas , Acetatos/síntesis química , Acetatos/farmacocinética , Administración Oral , Animales , Sitios de Unión , Compuestos de Bifenilo/química , Compuestos de Bifenilo/farmacología , Cristalografía por Rayos X , Diseño de Fármacos , Semivida , Humanos , Concentración 50 Inhibidora , Ratones , Microsomas/metabolismo , Simulación del Acoplamiento Molecular , PPAR delta/metabolismo , Estructura Terciaria de Proteína , Ratas , Estereoisomerismo , Relación Estructura-Actividad , Tiazoles/química , Tiazoles/metabolismo
12.
Ecotoxicol Environ Saf ; 148: 601-607, 2018 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-29127823

RESUMEN

The adsorption characteristics of Reactive Black 5 (RB5) and Cong Red (CR) onto Banana Peel Powder (BPP) from aqueous solution were investigated as a function of pH, contact time, initial dye concentration and temperature. The BPP was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) analysis. FTIR results revealed that hydroxyl (-OH), amine (-NH) and carboxyl (-CË­O) functional groups present on the surface of BPP. The SEM results show that BPP has an irregular and porous surface morphology which is adequate for dye adsorption. The equilibrium data were analyzed using Langmuir and Freundlich isotherm models. Experimental results were best represented by the Langmuir isotherm model. The adjustments of models were confirmed by the Chi-square (χ2) test and the correlation coefficients (R2). The maximum monolayer adsorption capacities of RB5 and CR on BPP calculated from Langmuir isotherm model were 49.2 and 164.6mg/g at pH 3.0 and 298K. Experimental data were also tested in terms of adsorption kinetics using pseudo-first-order and pseudo-second-order kinetic models. The results showed that the adsorption processes of both RB5 and CR followed well pseudo-second-order kinetic models. The calculated thermodynamic parameters ΔG°, ΔH° and ΔS° showed that the adsorption of RB5 and CR onto BPP was feasible, spontaneous and endothermic in the temperature range 298-318K. The RB5 and CR were desorbed from BPP using 0.1M NaOH. The recovery for both anionic dyes was found to be higher than 90%. Based on these it can be concluded that BPP can be used as an effective, low cost, and eco-friendly adsorbent for CR removal than RB5 from aqueous solution.


Asunto(s)
Colorantes/análisis , Rojo Congo/análisis , Musa/química , Naftalenosulfonatos/análisis , Contaminantes Químicos del Agua/análisis , Purificación del Agua/métodos , Adsorción , Frutas/química , Cinética , Modelos Teóricos , Polvos , Soluciones , Propiedades de Superficie
13.
Ecotoxicol Environ Saf ; 141: 226-234, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-28349874

RESUMEN

The present study is concerned with the batch adsorption of congo red (CR) from an aqueous solution using calcium alginate beads impregnated with nano-goethite (CABI nano-goethite) as an adsorbent. The optimum conditions for CR removal were determined by studying operational variables viz. pH, adsorbent dose, contact time, initial dye ion concentration and temperature. The CABI nano-goethite was characterized by Fourier transform infrared spectroscopy (FTIR), X- ray diffraction (XRD) and Scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) analysis. The CR sorption data onto CABI nano-goethite were described using Langmuir, Freundlich, Dubinin-Radushkevich and Temkin isotherm models. The results show that the best fit was achieved with the Langmuir isotherm model. The maximum adsorption capacity (181.1mg/g) of CR was occurred at pH 3.0. Kinetic studies showed that the adsorption followed a pseudo-second-order model. Desorption experiments were carried out to explore the feasibility of regenerating the adsorbent and the adsorbed CR from CABI nano-goethite. The best desorbing agent was 0.1M NaOH with an efficiency of 94% recovery. The thermodynamic parameters ΔG°, ΔH°, and ΔS° for the CR adsorption were determined by using adsorption capacities at five different temperatures (293, 303, 313, 323 and 303K). Results show that the adsorption process was endothermic and favoured at high temperature.


Asunto(s)
Alginatos/química , Rojo Congo/análisis , Compuestos de Hierro/química , Minerales/química , Contaminantes del Agua/análisis , Adsorción , Ácido Glucurónico/química , Ácidos Hexurónicos/química , Concentración de Iones de Hidrógeno , Cinética , Modelos Teóricos , Soluciones , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie , Temperatura , Termodinámica
14.
Chemistry ; 22(18): 6234-8, 2016 Apr 25.
Artículo en Inglés | MEDLINE | ID: mdl-26918409

RESUMEN

A carbonylative esterification reaction between aryl bromides and alcohols, promoted by Pd/C and NaF in the presence of oxiranes, has been developed. In this process, oxiranes serve as sources of carbon monoxide by their conversion to aldehydes through a palladium-promoted Meinwald rearrangement pathway. Intramolecular versions of this process serve as methods for the synthesis of lactones and phthalimides.


Asunto(s)
Alcoholes/química , Monóxido de Carbono/química , Compuestos Epoxi/química , Halógenos/química , Paladio/química , Ftalimidas/química , Catálisis , Esterificación , Estructura Molecular , Estereoisomerismo
15.
Ecotoxicol Environ Saf ; 128: 109-17, 2016 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-26921544

RESUMEN

Present research discussed the utilization of aminated pumpkin seed powder (APSP) as an adsorbent for methyl orange (MO) removal from aqueous solution. Batch sorption experiments were carried to evaluate the influence of pH, initial dye concentration, contact time, and temperature. The APSP was characterized by using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The experimental equilibrium adsorption data were fitted using two two-parameter models (Langmuir and Freundlich) and two three-parameter models (Sips and Toth). Langmuir and Sips isotherms provided the best model for MO adsorption data. The maximum monolayer sorption capacity was found to be 200.3mg/g based on the Langmuir isotherm model. The pseudo-first-order and pseudo-second-order model equations were used to analyze the kinetic data of the adsorption process and the data was fitted well with the pseudo-second-order kinetic model (R(2)>0.97). The calculated thermodynamic parameters such as ΔG(0), ΔH(0) and ΔS(0) from experimental data showed that the sorption of MO onto APSP was feasible, spontaneous and endothermic in the temperature range 298-318 K. The FTIR results revealed that amine and carboxyl functional groups present on the surface of APSP. The SEM results show that APSP has an irregular and porous surface which is adequate morphology for dye adsorption. Desorption experiments were carried to explore the feasibility of adsorbent regeneration and the adsorbed MO from APSP was desorbed using 0.1M NaOH with an efficiency of 93.5%. Findings of the present study indicated that APSP can be successfully used for removal of MO from aqueous solution.


Asunto(s)
Compuestos Azo/análisis , Cucurbita/química , Etanolamina/química , Semillas/química , Contaminantes Químicos del Agua/análisis , Adsorción , Concentración de Iones de Hidrógeno , Cinética , Microscopía Electrónica de Rastreo , Modelos Teóricos , Polvos , Soluciones , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie , Temperatura , Termodinámica
16.
BMC Complement Altern Med ; 15: 355, 2015 Oct 14.
Artículo en Inglés | MEDLINE | ID: mdl-26467297

RESUMEN

BACKGROUND: Satisfaction with medical doctor (MD) has been studied as a possible motivation for trying complementary medicine. This study aimed to explore the relationship between Korean outpatients' satisfaction with their MDs and their use of traditional Korean medicine (KM). METHODS: Data were drawn from the 2011 annual Korea Health Panel, a national representative sample. We analyzed the relationship between outpatients' use of KM and outpatients' satisfaction with MDs by using the responses of 9,753 outpatients, including 1,946 KM outpatients. The Andersen behavior model was applied to select the variables. The validity and reliability of the questionnaires were tested by Factor Analysis and Cronbach's alpha. Multiple logistic regression was used to evaluate five MD satisfaction indicators (patient's trust in MD, MD's careful listening, MD's sufficient explanation, MD's consultation time, and MD's respect for patient) and the overall satisfaction with the MD. RESULTS: There was no significant difference between the MD satisfaction of KM users and that of nonusers in any of the 5 indicators of MD satisfaction. When we controlled for all independent variables from the Anderson behavior model, however, the patients' overall dissatisfaction with MDs was associated with their use of KM (OR = 0.87,0.76-0.99). In addition, the more a patient was dissatisfied with the consultation time of their MD, the more they used KM (OR = 0.82, 072-0.94). CONCLUSIONS: Patients who were dissatisfied with their MD were more likely to use KM; the main indicator affecting MD dissatisfaction was the relatively short time of MD consultations. This could be one reason why KM plays a complementary role with conventional medicine in Korea.


Asunto(s)
Medicina Tradicional Coreana , Pacientes Ambulatorios/psicología , Satisfacción del Paciente , Relaciones Médico-Paciente , Adulto , Anciano , Terapias Complementarias/estadística & datos numéricos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Reproducibilidad de los Resultados , República de Corea , Encuestas y Cuestionarios , Factores de Tiempo
17.
Korean J Parasitol ; 53(6): 737-43, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26797442

RESUMEN

In order to examine the prevalence of Cryptosporidium infection in wild rodents and insectivores of South Korea and to assess their potential role as a source of human cryptosporidiosis, a total of 199 wild rodents and insectivore specimens were collected from 10 regions of South Korea and screened for Cryptosporidium infection over a period of 2 years (2012-2013). A nested-PCR amplification of Cryptosporidium oocyst wall protein (COWP) gene fragment revealed an overall prevalence of 34.2% (68/199). The sequence analysis of 18S rRNA gene locus of Cryptosporidium was performed from the fecal and cecum samples that tested positive by COWP amplification PCR. As a result, we identified 4 species/genotypes; chipmunk genotype I, cervine genotype I, C. muris, and a new genotype which is closely related to the bear genotype. The new genotype isolated from 12 Apodemus agrarius and 2 Apodemus chejuensis was not previously identified as known species or genotype, and therefore, it is supposed to be a novel genotype. In addition, the host spectrum of Cryptosporidium was extended to A. agrarius and Crosidura lasiura, which had not been reported before. In this study, we found that the Korean wild rodents and insectivores were infected with various Cryptosporidium spp. with large intra-genotypic variationa, indicating that they may function as potential reservoirs transmitting zoonotic Cryptosporidium to livestock and humans.


Asunto(s)
Animales Salvajes/parasitología , Criptosporidiosis/parasitología , Cryptosporidium/genética , Cryptosporidium/aislamiento & purificación , Eulipotyphla/parasitología , Enfermedades de los Roedores/parasitología , Animales , Cryptosporidium/clasificación , Heces/parasitología , Genotipo , Datos de Secuencia Molecular , Murinae , Filogenia , República de Corea
18.
J Org Chem ; 79(24): 12191-6, 2014 Dec 19.
Artículo en Inglés | MEDLINE | ID: mdl-25184856

RESUMEN

A chelation-assisted hydroesterification reaction of alkenes with sodium formate and alcohols that involves cooperative catalysis by Ru3(CO)12 and 2-pyridinemethanol is described. In this three-component coupling reaction, sodium formate serves as the carbon monoxide source.

19.
Heliyon ; 10(11): e32060, 2024 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-38882263

RESUMEN

Background: South Korea's Ministry of Health and Welfare has developed clinical pathways for Korean Medicine (KM-CPs). As part of this initiative, a panel comprising Korean Medicine doctors (KMD) was assembled. This implementation study aimed to preliminarily explore how KM-CP implementation affects the appropriateness and efficiency of the clinical process and its relation to attitude. Methods: Through random sampling, 311 KMDs were recruited as panelists to participate in two surveys. The surveys included information regarding the KM clinical environment and KM-CP implementation. A panel management program and educational materials were provided to KMDs between the two survey periods. Only 262 KMDs who responded to both surveys were included in the analysis. Three analyses were conducted: 1) descriptive analysis of the study variables, 2) panel analysis using the ordered logit regression model to elucidate the impact of KM-CP on the appropriateness and efficiency of the clinical process, and 3) ordered logit regression analysis of the association between KM-CP implementation and attitude. Results: More than two-thirds of the KMDs attempted to adopt KM-CP, with mostly positive perception expressed by these doctors. However, expectations and concerns coexist with the standardization of KM-CP. Cases in which KM-CPs were partially and mostly implemented respectively had negative and positive effects on the appropriateness and efficiency of the clinical process compared to those in which KM-CPs were not implemented. Compared to neutral attitude, positive and very positive attitudes tended to be associated with increased implementation of KM-CP. However, statistical significances were not observed. Conclusions: The impact of KM-CP on the clinical process and its association with attitude were found to be statistically unclear or inconsistent. Considering the study limitations and implications, we suggest a policy and academic strategies aimed at fostering improvement to enhance its utility.

20.
Chemosphere ; 353: 141524, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38403122

RESUMEN

The public and society have increasingly recognized numerous grave environmental issues, including water pollution, attributed to the rapid expansion of industrialization and agriculture. Renewable energy-driven catalytic advanced oxidation processes (AOPs) represent a green, sustainable, and environmentally friendly approach to meet the demands of environmental remediation. In this context, 2D transition metal dichalcogenides (TMDCs) piezoelectric materials, with their non-centrosymmetric crystal structure, exhibit unique features. They create dipole polarization, inducing a built-in electric field that generates polarized holes and electrons and triggers redox reactions, thereby facilitating the generation of reactive oxygen species for wastewater pollutant remediation. A broad spectrum of 2D TMDCs piezoelectric materials have been explored in self-integrated Fenton-like processes and persulfate activation processes. These materials offer a more simplistic and practical method than traditional approaches. Consequently, this review highlights recent advancements in 2D TMDCs piezoelectric catalysts and their roles in wastewater pollutant remediation through piezocatalytic-driven AOPs, such as Fenton-like processes and sulfate radicals-based oxidation processes.


Asunto(s)
Contaminantes Ambientales , Contaminantes Químicos del Agua , Aguas Residuales , Contaminantes Químicos del Agua/química , Metales , Oxidación-Reducción
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