Comparison of metabolic kinetics during high and low solids anaerobic digestion of fecal sludge.

High solids anaerobic digestion (HS-AD) is an attractive energy-producing technology; however, high total solids (TS) content may inhibit methanogens due to high volatile fatty acid (VFA) and total ammonia nitrogen concentrations. The objective of this paper is to quantify rate-limiting metabolic kinetic parameters to determine the influence of TS content during anaerobic digestion of fecal sludge. Two TS content: 11% and 17% microcosms were analyzed. Good performance was observed in both systems, with volatile solid (VS) removal greater than 50%, CH4 yield between 0.44 and 0.56 m3 CH4 /g VS added and cumulative CH4 production between 1.78 and 2.03 m3 CH4 /m3 digester-day. At 11% TS VFA consumption and VS removal had a positive correlation to CH4 production while the 17% TS microcosm had a negative correlation with both. This is the first study to determine the kinetic parameters for hydrolysis, VFA consumption, and methanogenesis during digestion of fecal sludge. These kinetic parameters are necessary in the design and operation of anaerobic digestion systems treating fecal sludge.

Controlling the COD removal of an A-stage pilot study with instrumentation and automatic process control.

The pursuit of fully autotrophic nitrogen removal via the anaerobic ammonium oxidation (anammox) pathway has led to an increased interest in carbon removal technologies, particularly the A-stage of the adsorption/bio-oxidation (A/B) process. The high-rate operation of the A-stage and lack of automatic process control often results in wide variations of chemical oxygen demand (COD) removal that can ultimately impact nitrogen removal in the downstream B-stage process. This study evaluated the use dissolved oxygen (DO) and mixed liquor suspended solids (MLSS) based automatic control strategies through the use of in situ on-line sensors in the A-stage of an A/B pilot study. The objective of using these control strategies was to reduce the variability of COD removal by the A-stage and thus the variability of the effluent C/N. The use of cascade DO control in the A-stage did not impact COD removal at the conditions tested in this study, likely because the bulk DO concentration (>0.5 mg/L) was maintained above the half saturation coefficient of heterotrophic organisms for DO. MLSS-based solids retention time (SRT) control, where MLSS was used as a surrogate for SRT, did not significantly reduce the effluent C/N variability but it was able to reduce COD removal variation in the A-stage by 90%.

Does the use of tubular digesters to treat livestock waste lower the risk of infection from Cryptosporidium parvum and Giardia lamblia?

Worldwide, high incidences of cryptosporidiosis and giardiasis are attributed to livestock waste. Quantitative microbial risk assessment can be used to estimate the risk of livestock related infections from Cryptosporidium parvum and Giardia lamblia. The objective of this paper was to assess the occupational and public health risks associated with management of raw and anaerobically digested livestock waste in two rural communities in Costa Rica based on fomite, soil and crop contamination and livestock waste management exposure pathways. Risks related to cattle waste were greater than swine waste due to cattle shedding more (oo)cysts. Cryptosporidium parvum also posed a greater risk than Giardia lamblia in all exposure pathways due to livestock shedding high loads of Cryptosporidium parvum oocysts and oocysts' lower inactivation rates during anaerobic digestion compared with Giardia lamblia cysts. The risk of infection from exposure to contaminated soil and crops was significantly lower for a community using tubular anaerobic digesters to treat livestock waste compared to a community where the untreated waste was applied to soil. The results indicate that treatment of livestock waste in small-scale tubular anaerobic digesters has the potential to significantly decrease the risk of infection below the World Health Organization's acceptable individual annual risk of infection (10-4).

Developing occupational and health susceptibility personas for wastewater personnel in the United States in the age of COVID-19.

Compared with the public, wastewater personnel, are at an increased risk of infection and illness from wastewater pathogens due to work-related tasks. Unfortunately, current risk assessment approaches do not consider individual personnel factors (e.g., age and health conditions) that may influence their susceptibility to a health effect. The objective of this study is to establish a baseline level of occupational and health factors among the wastewater personnel population, quantify these factors using a susceptibility evaluation scoring system, and examine relevant susceptibility features using the concept of "Personas." Using survey data from 246 respondents and public health risk data on COVID-19 from the CDC, personnel clustered into three persona groups: "low susceptibility," "high occupational susceptibility," and "high health susceptibility." Results highlight the intersectionality between gender, age, underlying health conditions, job tasks, and level of exposure to wastewater and provide context for incorporating individual variables into risk assessment methodologies with the goal of protecting this essential workforce. PRACTITIONER POINTS: A risk assessment framework that combines health and occupational susceptibility factors was developed for wastewater treatment plant personnel. Wastewater personnel clustered into three persona groups: "low susceptibility," "high occupational susceptibility," and "high health susceptibility." The intersectionality between job related activities and individual health provides a holistic approach to risk assessment for wastewater personnel.

Adsorption of perfluorooctanoic acid from water by pH-modulated Brönsted acid and base sites in mesoporous hafnium oxide ceramics.

Per- and polyfluoroalkyl substances (PFAS) are increasingly appearing in drinking water sources globally. Our work focuses specifically on the adsorption of the legacy perfluorooctanoic acid (PFOA) using mesoporous hafnium oxide (MHO) ceramic synthesized via a sol-gel process. Experiments were performed at varying pH to determine the effect of surface charge on adsorption capacity of PFOA by MHO, and to postulate adsorption behavior. At pH 2.3, the adsorption capacity of PFOA on MHO was 20.9 mg/g, whereas at a higher pH of 6.3, it was much lower at 9.2 mg/g. This was due to increased coulombic attractions at lower pH between the positively charged conjugate acid active sites on MHO surface and negatively charged deprotonated PFOA anion in solution. After adsorption, the solid MHO was regenerated via calcination, reducing the amount of toxic solid waste to be disposed since the adsorbent is regenerated, and the PFOA is completely removed.

Viability and fate of Cryptosporidium parvum and Giardia lamblia in tubular anaerobic digesters.

In many developing countries where pathogenic diseases of animal waste origin, such as giardiasis and cryptosporidiosis, are often prevalent, facilities are limited to treat livestock waste. However, household-scale anaerobic digesters are currently being promoted for bioenergy production from livestock manure. Since the effluent is often used as a fertilizer for food crops, it is critical to understand the effect of environmental conditions within household-scale digesters on the viability of Cryptosporidium parvum oocysts and Giardia lamblia cysts. In this study, key environmental parameters affecting (oo)cyst inactivation were measured in four tubular anaerobic digesters, which are a type of household-scale digester promoted for treatment of swine waste in rural Costa Rica. Interviews and participant observations were used to understand digester operation and maintenance procedures. Ambient temperatures (21-24°C), near-neutral pH, total ammonia nitrogen (TAN) concentrations<250 mg/L and hydraulic retention times (HRTs) between 23 and 180 days were observed. Laboratory (oo)cysts inactivation studies were performed in bench-scale digesters, which were maintained under conditions similar to those observed in the field. Apparent first-order inactivation rate coefficients for Giardia lamblia and Cryptosporidium parvum were 0.155 ± 0.041 and 0.054 ± 0.006 day(-1), respectively. Temperature and volatile fatty acids were the main factors contributing to Cryptosporidium parvum and Giardia lamblia inactivation. A mathematical model was developed that predicts the concentration of (oo)cysts in the liquid effluent of tubular digesters like those observed in Costa Rica. A mathematical model was developed that predicts the concentration of (oo)cysts in the liquid effluent of tubular digesters like those observed in Costa Rica. Two dimensionless groups can be used to predict the performance of the digesters for inactivating pathogens; both dimensionless groups depend upon the average HRT in the digester. This is the first study to combine mathematical modeling with qualitative analysis, field and laboratory studies to predict the concentrations of (oo)cysts in tubular digester effluents.

Effect of solids retention time on the bioavailability of organic carbon in anaerobically digested swine waste.

Anaerobic digestion (AD) can be used to stabilize and produce energy from livestock waste; however, digester effluents may require further treatment to remove nitrogen. This paper quantifies the effects of varying solids retention time (SRT) methane yield, volatile solids (VS) reduction and organic carbon bioavailability for denitrification during swine waste AD. Four bench-scale anaerobic digesters, with SRTs of 14, 21, 28 and 42 days, operated with swine waste feed. Effluent organic carbon bioavailability was measured using anoxic microcosms and respirometry. Excellent performance was observed for all four digesters, with >60% VS removal and CH4 yields between 0.1 and 0.3(m(3)CH4)/(kg VS added). Organic carbon in the centrate as an internal organic carbon source for denitrification supported maximum specific denitrification rates between 47 and 56(mg NO3(-)-N)/(g VSS h). The digester with the 21-day SRT had the highest CH4 yield and maximum specific denitrification rates.